ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日：2016年5月1日

Clone Information

BLAST Search Result

Clone Name basd2i14
Clone Library Name barley_pub
No. Definition Score
(bits)		 E
Value
1gb|AC137744.2| Oryza sativa (japonica cultivar-group) chromosome... 410 e-111
2dbj|AP008217.1| Oryza sativa (japonica cultivar-group) genomic D... 410 e-111
3dbj|AK066319.1| Oryza sativa (japonica cultivar-group) cDNA clon... 410 e-111
4gb|DP000010.1| Oryza sativa (japonica cultivar-group) chromosome... 410 e-111
5dbj|AP006712.1| Lotus japonicus genomic DNA, chromosome 2, clone... 80 6e-12
6dbj|AB120535.2| Nicotiana tabacum NtCAPL mRNA, complete cds 76 9e-11
7gb|BT013845.1| Lycopersicon esculentum clone 132802F, mRNA sequence 68 2e-08
8gb|AC136451.9| Medicago truncatula clone mth2-17d19, complete se... 62 1e-06
9gb|AC097500.3| Homo sapiens BAC clone RP11-335G13 from 2, comple... 44 0.33
10dbj|AK132982.1| Mus musculus adult male testis cDNA, RIKEN full-... 42 1.3
11gb|AC160415.4| Mus musculus 10 BAC RP24-410I24 (Roswell Park Can... 42 1.3
12gb|AC129935.8| Mus musculus chromosome 15, clone RP23-269P8, com... 42 1.3
13gb|AC106834.9| Mus musculus chromosome 19, clone RP24-132H7, com... 40 5.2
14gb|AC162612.2| Mus musculus chromosome 19, clone RP24-334K20, co... 40 5.2
15gb|AC110090.6| Mus musculus BAC clone RP23-27L4 from chromosome ... 40 5.2
16gb|AC015658.11| Mus musculus chromosome 1, clone RP23-130F18, co... 40 5.2
17gb|AC131565.1| Homo sapiens chromosome 5 clone RP11-1273L8, comp... 40 5.2
18gb|AC114959.2| Homo sapiens chromosome 5 clone RP11-170L13, comp... 40 5.2
19gb|AC011368.5| Homo sapiens chromosome 5 clone CTB-104K23, compl... 40 5.2
20gb|AC022410.5| Homo sapiens chromosome 5 clone CTB-17K15, comple... 40 5.2
21gb|AC140677.1| Homo sapiens chromosome 5 clone CTC-778L3, comple... 40 5.2
22emb|BX119975.7| Zebrafish DNA sequence from clone CH211-200A16 i... 40 5.2
23gb|AC116475.7| Mus musculus chromosome 8, clone RP23-386N2, comp... 40 5.2 
>gb|AC137744.2| Oryza sativa (japonica cultivar-group) chromosome 11 BAC clone
             OSJNBa0074L11, complete sequence
          Length = 180703

 Score =  410 bits (207), Expect = e-111
 Identities = 342/387 (88%)
 Strand = Plus / Minus

                                                                         
Query: 1     agacttatgctgacatcaccccagttaggatttctgacaattcggttacggcgttcgtgt 60
             |||| |||||||||||||||||||||||||| ||||||||||| || || || || || |
Sbjct: 61879 agacgtatgctgacatcaccccagttaggatctctgacaattcagtgaccgcatttgtat 61820

                                                                         
Query: 61    cagttatgaggggttgtaacaatatgtgctcgttttgcattgttcccttcactagaggca 120
             || ||||||||||||| |||||||||||||| ||||| || ||||| ||||||||||| |
Sbjct: 61819 caattatgaggggttgcaacaatatgtgctccttttgtatcgttcctttcactagaggta 61760

                                                                         
Query: 121   gggagaggtcacgcccagtatcttctgttgtccgagaagttggtgagctctggtatgctg 180
             |||| ||||| || ||||| || ||  |||||||||||||||| ||||||||| | || |
Sbjct: 61759 gggaaaggtcccgtccagtgtcctcaattgtccgagaagttggggagctctggaaagcag 61700

                                                                         
Query: 181   gcgtaaaagaagtaatgcttcttggtcagaatgtaaacagttataatgatacttctgaag 240
             |||| || ||||||||||||||||||||||| |||||||||||||| || ||||||||||
Sbjct: 61699 gcgtgaaggaagtaatgcttcttggtcagaacgtaaacagttataacgacacttctgaag 61640

                                                                         
Query: 241   ttgaggagttagagcctggtaaaaactggcagctcagcgaaggattttccagcaggtgca 300
             ||||||| ||||| ||||||||||||||| ||||||| ||||| ||||| |||| |||||
Sbjct: 61639 ttgaggaattagaacctggtaaaaactgggagctcagtgaagggttttctagcatgtgca 61580

                                                                         
Query: 301   aagtgaagaatacggggttgcgctttgccgatctcctggatcagttgtctctggaatacc 360
             | |||||||||| ||||||||| ||||| ||||||||||||||||| ||| |||||||||
Sbjct: 61579 aggtgaagaatatggggttgcgttttgctgatctcctggatcagttatctttggaatacc 61520

                                        
Query: 361   ctgagatgcgattcaggtttacctctc 387
             |||||||||| ||||||||||||||||
Sbjct: 61519 ctgagatgcggttcaggtttacctctc 61493
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>dbj|AP008217.1| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 11, complete
                sequence
          Length = 28386948

 Score =  410 bits (207), Expect = e-111
 Identities = 342/387 (88%)
 Strand = Plus / Minus

                                                                            
Query: 1        agacttatgctgacatcaccccagttaggatttctgacaattcggttacggcgttcgtgt 60
                |||| |||||||||||||||||||||||||| ||||||||||| || || || || || |
Sbjct: 21709826 agacgtatgctgacatcaccccagttaggatctctgacaattcagtgaccgcatttgtat 21709767

                                                                            
Query: 61       cagttatgaggggttgtaacaatatgtgctcgttttgcattgttcccttcactagaggca 120
                || ||||||||||||| |||||||||||||| ||||| || ||||| ||||||||||| |
Sbjct: 21709766 caattatgaggggttgcaacaatatgtgctccttttgtatcgttcctttcactagaggta 21709707

                                                                            
Query: 121      gggagaggtcacgcccagtatcttctgttgtccgagaagttggtgagctctggtatgctg 180
                |||| ||||| || ||||| || ||  |||||||||||||||| ||||||||| | || |
Sbjct: 21709706 gggaaaggtcccgtccagtgtcctcaattgtccgagaagttggggagctctggaaagcag 21709647

                                                                            
Query: 181      gcgtaaaagaagtaatgcttcttggtcagaatgtaaacagttataatgatacttctgaag 240
                |||| || ||||||||||||||||||||||| |||||||||||||| || ||||||||||
Sbjct: 21709646 gcgtgaaggaagtaatgcttcttggtcagaacgtaaacagttataacgacacttctgaag 21709587

                                                                            
Query: 241      ttgaggagttagagcctggtaaaaactggcagctcagcgaaggattttccagcaggtgca 300
                ||||||| ||||| ||||||||||||||| ||||||| ||||| ||||| |||| |||||
Sbjct: 21709586 ttgaggaattagaacctggtaaaaactgggagctcagtgaagggttttctagcatgtgca 21709527

                                                                            
Query: 301      aagtgaagaatacggggttgcgctttgccgatctcctggatcagttgtctctggaatacc 360
                | |||||||||| ||||||||| ||||| ||||||||||||||||| ||| |||||||||
Sbjct: 21709526 aggtgaagaatatggggttgcgttttgctgatctcctggatcagttatctttggaatacc 21709467

                                           
Query: 361      ctgagatgcgattcaggtttacctctc 387
                |||||||||| ||||||||||||||||
Sbjct: 21709466 ctgagatgcggttcaggtttacctctc 21709440
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>dbj|AK066319.1| Oryza sativa (japonica cultivar-group) cDNA clone:J013067A17, full
            insert sequence
          Length = 2156

 Score =  410 bits (207), Expect = e-111
 Identities = 342/387 (88%)
 Strand = Plus / Plus

                                                                        
Query: 1    agacttatgctgacatcaccccagttaggatttctgacaattcggttacggcgttcgtgt 60
            |||| |||||||||||||||||||||||||| ||||||||||| || || || || || |
Sbjct: 714  agacgtatgctgacatcaccccagttaggatctctgacaattcagtgaccgcatttgtat 773

                                                                        
Query: 61   cagttatgaggggttgtaacaatatgtgctcgttttgcattgttcccttcactagaggca 120
            || ||||||||||||| |||||||||||||| ||||| || ||||| ||||||||||| |
Sbjct: 774  caattatgaggggttgcaacaatatgtgctccttttgtatcgttcctttcactagaggta 833

                                                                        
Query: 121  gggagaggtcacgcccagtatcttctgttgtccgagaagttggtgagctctggtatgctg 180
            |||| ||||| || ||||| || ||  |||||||||||||||| ||||||||| | || |
Sbjct: 834  gggaaaggtcccgtccagtgtcctcaattgtccgagaagttggggagctctggaaagcag 893

                                                                        
Query: 181  gcgtaaaagaagtaatgcttcttggtcagaatgtaaacagttataatgatacttctgaag 240
            |||| || ||||||||||||||||||||||| |||||||||||||| || ||||||||||
Sbjct: 894  gcgtgaaggaagtaatgcttcttggtcagaacgtaaacagttataacgacacttctgaag 953

                                                                        
Query: 241  ttgaggagttagagcctggtaaaaactggcagctcagcgaaggattttccagcaggtgca 300
            ||||||| ||||| ||||||||||||||| ||||||| ||||| ||||| |||| |||||
Sbjct: 954  ttgaggaattagaacctggtaaaaactgggagctcagtgaagggttttctagcatgtgca 1013

                                                                        
Query: 301  aagtgaagaatacggggttgcgctttgccgatctcctggatcagttgtctctggaatacc 360
            | |||||||||| ||||||||| ||||| ||||||||||||||||| ||| |||||||||
Sbjct: 1014 aggtgaagaatatggggttgcgttttgctgatctcctggatcagttatctttggaatacc 1073

                                       
Query: 361  ctgagatgcgattcaggtttacctctc 387
            |||||||||| ||||||||||||||||
Sbjct: 1074 ctgagatgcggttcaggtttacctctc 1100
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>gb|DP000010.1| Oryza sativa (japonica cultivar-group) chromosome 11, complete sequence
          Length = 28369397

 Score =  410 bits (207), Expect = e-111
 Identities = 342/387 (88%)
 Strand = Plus / Minus

                                                                            
Query: 1        agacttatgctgacatcaccccagttaggatttctgacaattcggttacggcgttcgtgt 60
                |||| |||||||||||||||||||||||||| ||||||||||| || || || || || |
Sbjct: 22020025 agacgtatgctgacatcaccccagttaggatctctgacaattcagtgaccgcatttgtat 22019966

                                                                            
Query: 61       cagttatgaggggttgtaacaatatgtgctcgttttgcattgttcccttcactagaggca 120
                || ||||||||||||| |||||||||||||| ||||| || ||||| ||||||||||| |
Sbjct: 22019965 caattatgaggggttgcaacaatatgtgctccttttgtatcgttcctttcactagaggta 22019906

                                                                            
Query: 121      gggagaggtcacgcccagtatcttctgttgtccgagaagttggtgagctctggtatgctg 180
                |||| ||||| || ||||| || ||  |||||||||||||||| ||||||||| | || |
Sbjct: 22019905 gggaaaggtcccgtccagtgtcctcaattgtccgagaagttggggagctctggaaagcag 22019846

                                                                            
Query: 181      gcgtaaaagaagtaatgcttcttggtcagaatgtaaacagttataatgatacttctgaag 240
                |||| || ||||||||||||||||||||||| |||||||||||||| || ||||||||||
Sbjct: 22019845 gcgtgaaggaagtaatgcttcttggtcagaacgtaaacagttataacgacacttctgaag 22019786

                                                                            
Query: 241      ttgaggagttagagcctggtaaaaactggcagctcagcgaaggattttccagcaggtgca 300
                ||||||| ||||| ||||||||||||||| ||||||| ||||| ||||| |||| |||||
Sbjct: 22019785 ttgaggaattagaacctggtaaaaactgggagctcagtgaagggttttctagcatgtgca 22019726

                                                                            
Query: 301      aagtgaagaatacggggttgcgctttgccgatctcctggatcagttgtctctggaatacc 360
                | |||||||||| ||||||||| ||||| ||||||||||||||||| ||| |||||||||
Sbjct: 22019725 aggtgaagaatatggggttgcgttttgctgatctcctggatcagttatctttggaatacc 22019666

                                           
Query: 361      ctgagatgcgattcaggtttacctctc 387
                |||||||||| ||||||||||||||||
Sbjct: 22019665 ctgagatgcggttcaggtttacctctc 22019639
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>dbj|AP006712.1| Lotus japonicus genomic DNA, chromosome 2, clone:LjT17E09, TM0627,
             complete sequence
          Length = 101220

 Score = 79.8 bits (40), Expect = 6e-12
 Identities = 61/68 (89%)
 Strand = Plus / Minus

                                                                         
Query: 66    atgaggggttgtaacaatatgtgctcgttttgcattgttcccttcactagaggcagggag 125
             ||||||||||| |||||||||||||| ||||| ||||| || |||||||||||||| |||
Sbjct: 84981 atgaggggttgcaacaatatgtgctcattttgtattgtacctttcactagaggcagagag 84922

                     
Query: 126   aggtcacg 133
              |||||||
Sbjct: 84921 cggtcacg 84914



 Score = 48.1 bits (24), Expect = 0.021
 Identities = 45/52 (86%)
 Strand = Plus / Minus

                                                                 
Query: 186   aaagaagtaatgcttcttggtcagaatgtaaacagttataatgatacttctg 237
             ||||||||||  |||||||| ||||| || ||||| ||||||||||| ||||
Sbjct: 84861 aaagaagtaacacttcttggccagaacgtgaacagctataatgatacatctg 84810
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>dbj|AB120535.2| Nicotiana tabacum NtCAPL mRNA, complete cds
          Length = 2160

 Score = 75.8 bits (38), Expect = 9e-11
 Identities = 65/74 (87%)
 Strand = Plus / Plus

                                                                       
Query: 66  atgaggggttgtaacaatatgtgctcgttttgcattgttcccttcactagaggcagggag 125
           ||||||||||| || ||||||||||| || ||||||||||||||||||||||| || || 
Sbjct: 891 atgaggggttgcaataatatgtgctcattctgcattgttcccttcactagagggagagaa 950

                         
Query: 126 aggtcacgcccagt 139
            |||| ||||||||
Sbjct: 951 cggtctcgcccagt 964



 Score = 54.0 bits (27), Expect = 3e-04
 Identities = 42/47 (89%)
 Strand = Plus / Plus

                                                           
Query: 198  cttcttggtcagaatgtaaacagttataatgatacttctgaagttga 244
            |||||||| ||||||||||| || ||||||||||| |||| ||||||
Sbjct: 1023 cttcttggccagaatgtaaatagctataatgatacatctggagttga 1069
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>gb|BT013845.1| Lycopersicon esculentum clone 132802F, mRNA sequence
          Length = 2181

 Score = 67.9 bits (34), Expect = 2e-08
 Identities = 64/74 (86%)
 Strand = Plus / Plus

                                                                       
Query: 66  atgaggggttgtaacaatatgtgctcgttttgcattgttcccttcactagaggcagggag 125
           ||||||||||| || |||||||| || || ||||||||||||||||||||||| || || 
Sbjct: 891 atgaggggttgcaataatatgtgttcattctgcattgttcccttcactagagggagagaa 950

                         
Query: 126 aggtcacgcccagt 139
            |||| ||||||||
Sbjct: 951 cggtctcgcccagt 964



 Score = 40.1 bits (20), Expect = 5.2
 Identities = 41/48 (85%)
 Strand = Plus / Plus

                                                            
Query: 197  gcttcttggtcagaatgtaaacagttataatgatacttctgaagttga 244
            |||||| || || |||||||| || ||||||||||| |||| ||||||
Sbjct: 1022 gcttctcggccaaaatgtaaatagctataatgatacatctggagttga 1069
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>gb|AC136451.9| Medicago truncatula clone mth2-17d19, complete sequence
          Length = 132473

 Score = 61.9 bits (31), Expect = 1e-06
 Identities = 52/59 (88%)
 Strand = Plus / Minus

                                                                        
Query: 60    tcagttatgaggggttgtaacaatatgtgctcgttttgcattgttcccttcactagagg 118
             ||||| ||||||||||| ||||||||||| |||||||| ||||| || || ||||||||
Sbjct: 30446 tcagtgatgaggggttgcaacaatatgtgttcgttttgtattgtaccttttactagagg 30388
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>gb|AC097500.3| Homo sapiens BAC clone RP11-335G13 from 2, complete sequence
          Length = 180215

 Score = 44.1 bits (22), Expect = 0.33
 Identities = 22/22 (100%)
 Strand = Plus / Minus

                                    
Query: 191    agtaatgcttcttggtcagaat 212
              ||||||||||||||||||||||
Sbjct: 167239 agtaatgcttcttggtcagaat 167218
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>dbj|AK132982.1| Mus musculus adult male testis cDNA, RIKEN full-length enriched
            library, clone:4930542I17 product:unclassifiable, full
            insert sequence
          Length = 2311

 Score = 42.1 bits (21), Expect = 1.3
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                                 
Query: 219  agttataatgatacttctgaa 239
            |||||||||||||||||||||
Sbjct: 2082 agttataatgatacttctgaa 2102
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>gb|AC160415.4| Mus musculus 10 BAC RP24-410I24 (Roswell Park Cancer Institute
             (C57BL/6J Male) Mouse BAC Library) complete sequence
          Length = 144561

 Score = 42.1 bits (21), Expect = 1.3
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                                  
Query: 195   atgcttcttggtcagaatgta 215
             |||||||||||||||||||||
Sbjct: 67431 atgcttcttggtcagaatgta 67451
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>gb|AC129935.8| Mus musculus chromosome 15, clone RP23-269P8, complete sequence
          Length = 183894

 Score = 42.1 bits (21), Expect = 1.3
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                                   
Query: 219    agttataatgatacttctgaa 239
              |||||||||||||||||||||
Sbjct: 122075 agttataatgatacttctgaa 122095
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>gb|AC106834.9| Mus musculus chromosome 19, clone RP24-132H7, complete sequence
          Length = 177277

 Score = 40.1 bits (20), Expect = 5.2
 Identities = 23/24 (95%)
 Strand = Plus / Minus

                                     
Query: 219   agttataatgatacttctgaagtt 242
             ||||||||||||| ||||||||||
Sbjct: 14585 agttataatgataattctgaagtt 14562
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>gb|AC162612.2| Mus musculus chromosome 19, clone RP24-334K20, complete sequence
          Length = 145561

 Score = 40.1 bits (20), Expect = 5.2
 Identities = 23/24 (95%)
 Strand = Plus / Minus

                                      
Query: 219    agttataatgatacttctgaagtt 242
              ||||||||||||| ||||||||||
Sbjct: 118399 agttataatgataattctgaagtt 118376
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>gb|AC110090.6| Mus musculus BAC clone RP23-27L4 from chromosome 1, complete sequence
          Length = 218518

 Score = 40.1 bits (20), Expect = 5.2
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                  
Query: 207    cagaatgtaaacagttataa 226
              ||||||||||||||||||||
Sbjct: 192008 cagaatgtaaacagttataa 192027
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>gb|AC015658.11| Mus musculus chromosome 1, clone RP23-130F18, complete sequence
          Length = 177016

 Score = 40.1 bits (20), Expect = 5.2
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                  
Query: 207    cagaatgtaaacagttataa 226
              ||||||||||||||||||||
Sbjct: 170668 cagaatgtaaacagttataa 170649
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>gb|AC131565.1| Homo sapiens chromosome 5 clone RP11-1273L8, complete sequence
          Length = 181283

 Score = 40.1 bits (20), Expect = 5.2
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                  
Query: 219    agttataatgatacttctga 238
              ||||||||||||||||||||
Sbjct: 126931 agttataatgatacttctga 126950
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>gb|AC114959.2| Homo sapiens chromosome 5 clone RP11-170L13, complete sequence
          Length = 163805

 Score = 40.1 bits (20), Expect = 5.2
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 219   agttataatgatacttctga 238
             ||||||||||||||||||||
Sbjct: 55535 agttataatgatacttctga 55516
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>gb|AC011368.5| Homo sapiens chromosome 5 clone CTB-104K23, complete sequence
          Length = 113582

 Score = 40.1 bits (20), Expect = 5.2
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                
Query: 219  agttataatgatacttctga 238
            ||||||||||||||||||||
Sbjct: 6693 agttataatgatacttctga 6674
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>gb|AC022410.5| Homo sapiens chromosome 5 clone CTB-17K15, complete sequence
          Length = 68234

 Score = 40.1 bits (20), Expect = 5.2
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                 
Query: 219   agttataatgatacttctga 238
             ||||||||||||||||||||
Sbjct: 35063 agttataatgatacttctga 35082
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>gb|AC140677.1| Homo sapiens chromosome 5 clone CTC-778L3, complete sequence
          Length = 121010

 Score = 40.1 bits (20), Expect = 5.2
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 219   agttataatgatacttctga 238
             ||||||||||||||||||||
Sbjct: 56472 agttataatgatacttctga 56453
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>emb|BX119975.7| Zebrafish DNA sequence from clone CH211-200A16 in linkage group 8,
              complete sequence
          Length = 154904

 Score = 40.1 bits (20), Expect = 5.2
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                  
Query: 255    cctggtaaaaactggcagct 274
              ||||||||||||||||||||
Sbjct: 130888 cctggtaaaaactggcagct 130869
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>gb|AC116475.7| Mus musculus chromosome 8, clone RP23-386N2, complete sequence
          Length = 184741

 Score = 40.1 bits (20), Expect = 5.2
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                 
Query: 78    aacaatatgtgctcgttttg 97
             ||||||||||||||||||||
Sbjct: 84163 aacaatatgtgctcgttttg 84182


  Database: nt
    Posted date:  May 29, 2006 11:10 AM
  Number of letters in database: 3,984,495,279
  Number of sequences in database:  917,343
  
  Database: /shigen/export/home/twatanab/db/nt/nt.01
    Posted date:  May 29, 2006 11:16 AM
  Number of letters in database: 3,988,174,986
  Number of sequences in database:  835,257
  
  Database: /shigen/export/home/twatanab/db/nt/nt.02
    Posted date:  May 29, 2006 11:21 AM
  Number of letters in database: 3,991,246,324
  Number of sequences in database:  771,481
  
  Database: /shigen/export/home/twatanab/db/nt/nt.03
    Posted date:  May 29, 2006 11:27 AM
  Number of letters in database: 3,990,718,311
  Number of sequences in database:  977,174
  
  Database: /shigen/export/home/twatanab/db/nt/nt.04
    Posted date:  May 29, 2006 11:29 AM
  Number of letters in database: 1,278,410,368
  Number of sequences in database:  400,813
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 3,004,384
Number of Sequences: 3902068
Number of extensions: 3004384
Number of successful extensions: 53978
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 23
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 53919
Number of HSP's gapped (non-prelim): 59
length of query: 387
length of database: 17,233,045,268
effective HSP length: 22
effective length of query: 365
effective length of database: 17,147,199,772
effective search space: 6258727916780
effective search space used: 6258727916780
T: 0
A: 0
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)
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