ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | rbasd27h14 |
|---|---|
| Clone Library Name | barley_pub |
>TPP1_SCHPO (P78875) Trehalose-phosphatase (EC 3.1.3.12) (Trehalose 6-phosphate| phosphatase) (TPP) Length = 817 Score = 70.9 bits (172), Expect(2) = 1e-13 Identities = 48/135 (35%), Positives = 75/135 (55%), Gaps = 3/135 (2%) Frame = -1 Query: 653 KDADPDFGSCQAKELHEHLES-VLSNEPVSVKADVNHVEVKPQGVSKGLVAKRMLSAMQA 477 + ADP+ G+ QA E LE V S V + ++EV+P ++KG + K++LS+ Sbjct: 688 RKADPENGAFQALECEALLEELVCSKYDVEIMRGKANLEVRPSSINKGGIVKQILSSYPE 747 Query: 476 RGLLPDFILCIGDDRSDEDMFEVITTAVDGPYLNPEAKVFACTVG--RKPSKAKYYLDDT 303 L P FI C GDDR+DEDMF + +N E FA T+G +K S A + + D Sbjct: 748 DSL-PSFIFCAGDDRTDEDMFRSLH---KNTRINKETS-FAVTIGSDKKLSIADWCIADP 802 Query: 302 ADIVRLIQALANVSD 258 A+++ ++ LAN ++ Sbjct: 803 ANVIDILADLANFTN 817 Score = 25.0 bits (53), Expect(2) = 1e-13 Identities = 10/24 (41%), Positives = 14/24 (58%) Frame = -3 Query: 711 ETTDGSVIEDKETAIVWCYKGRRP 640 + T GS IE+K A+ W Y+ P Sbjct: 669 DRTQGSSIEEKRCAMTWHYRKADP 692
>TPS2_YEAST (P31688) Trehalose-phosphatase (EC 3.1.3.12) (Trehalose 6-phosphate| phosphatase) (TPP) (Trehalose synthase complex catalytic subunit TPS2) Length = 896 Score = 58.9 bits (141), Expect = 1e-08 Identities = 33/95 (34%), Positives = 50/95 (52%), Gaps = 14/95 (14%) Frame = -1 Query: 641 PDFGSCQAKELHEHLESVLSNEPVSVKADVNHVEVKPQGVSKGLVAKRM----------- 495 P+ G AKEL E L S + + V ++EV+P+ V+KG + KR+ Sbjct: 700 PELGEFHAKELKEKLLSFTDDFDLEVMDGKANIEVRPRFVNKGEIVKRLVWHQHGKPQDM 759 Query: 494 ---LSAMQARGLLPDFILCIGDDRSDEDMFEVITT 399 +S + +PDF+LC+GDD +DEDMF + T Sbjct: 760 LKGISEKLPKDEMPDFVLCLGDDFTDEDMFRQLNT 794
>OTSB_SALTY (Q9L894) Trehalose-phosphatase (EC 3.1.3.12) (Trehalose 6-phosphate| phosphatase) (TPP) Length = 267 Score = 35.0 bits (79), Expect = 0.23 Identities = 26/99 (26%), Positives = 43/99 (43%) Frame = -1 Query: 545 VEVKPQGVSKGLVAKRMLSAMQARGLLPDFILCIGDDRSDEDMFEVITTAVDGPYLNPEA 366 VE+KP+G +KG + G +P F +GDD +DE F V+ A Sbjct: 166 VEIKPKGTNKGEAIAAFMQEAPFAGRIPVF---VGDDLTDEAGFGVVN----------HA 212 Query: 365 KVFACTVGRKPSKAKYYLDDTADIVRLIQALANVSDEVQ 249 + VG ++A + L+ D+ R ++ + E Q Sbjct: 213 GGISVKVGVGATQAAWRLESVPDVWRWLEQINYPQQEQQ 251
>SYC_METCA (Q60BG8) Cysteinyl-tRNA synthetase (EC 6.1.1.16) (Cysteine--tRNA| ligase) (CysRS) Length = 465 Score = 35.0 bits (79), Expect = 0.23 Identities = 28/91 (30%), Positives = 43/91 (47%), Gaps = 1/91 (1%) Frame = -1 Query: 476 RGLLPDFILCIGDDRSDEDMFEVITTAVDGPYLNPEAKVFACTVGRKPSKAKYYLD-DTA 300 RGL PD L +G+DR DE A+ + PEA + R+ ++ K A Sbjct: 324 RGLAPDLPLPLGEDR-DEGFSSRFADAMQDDFNTPEAIAVLFDLAREINRHKAAEPAKAA 382 Query: 299 DIVRLIQALANVSDEVQGGPPSYFFACAGTS 207 + +++L NV +Q P +YF + AG S Sbjct: 383 ALGATLKSLGNVLGLLQTDPEAYFKSDAGGS 413
>TPP_ENCCU (Q8SSL0) Probable trehalose-phosphatase (EC 3.1.3.12) (Trehalose| 6-phosphate phosphatase) (TPP) Length = 718 Score = 33.5 bits (75), Expect = 0.66 Identities = 20/62 (32%), Positives = 29/62 (46%) Frame = -1 Query: 458 FILCIGDDRSDEDMFEVITTAVDGPYLNPEAKVFACTVGRKPSKAKYYLDDTADIVRLIQ 279 F+LC GDD +DEDMF+V K + VG + + A Y + D + L+ Sbjct: 668 FVLCAGDDVADEDMFDV-------------CKGYTIKVGDQSTSAAYRVKDPENFRMLLG 714 Query: 278 AL 273 L Sbjct: 715 RL 716
>PYRG_MYCCT (Q48965) CTP synthase (EC 6.3.4.2) (UTP--ammonia ligase) (CTP| synthetase) Length = 532 Score = 32.7 bits (73), Expect = 1.1 Identities = 25/80 (31%), Positives = 39/80 (48%), Gaps = 1/80 (1%) Frame = -1 Query: 494 LSAMQARGLLPDFILCIGDDRSDEDMFEVITTAVDGPYLNPEAKVFACTVGRKP-SKAKY 318 + AM + G+ PD I+C D S +D+ E I+ + P N + ++ R P + AK Sbjct: 196 VKAMASLGIQPDVIVCRSDSSSPKDIKEKISLFCNVPITNIIDAIDQDSIYRVPLALAKQ 255 Query: 317 YLDDTADIVRLIQALANVSD 258 L D I+ +Q AN D Sbjct: 256 NLQDI--IIEQLQLKANAID 273
>OTSB_ECOLI (P31678) Trehalose-phosphatase (EC 3.1.3.12) (Trehalose 6-phosphate| phosphatase) (TPP) Length = 266 Score = 32.3 bits (72), Expect = 1.5 Identities = 18/47 (38%), Positives = 24/47 (51%) Frame = -1 Query: 545 VEVKPQGVSKGLVAKRMLSAMQARGLLPDFILCIGDDRSDEDMFEVI 405 VE+KP+G SKG + G P F +GDD +DE F V+ Sbjct: 166 VEIKPRGTSKGEAIAAFMQEAPFIGRTPVF---LGDDLTDESGFAVV 209
>PALH_CANAL (Q59YK4) pH-response regulator protein palH/RIM21| Length = 529 Score = 31.2 bits (69), Expect = 3.3 Identities = 21/53 (39%), Positives = 26/53 (49%) Frame = -2 Query: 706 NGRVSHRGQGDSNRLVLQRTPTLILDLAKPRSSMNTWRVSFPTSRYRSKLT*T 548 NG H Q S+RL+ +PT KP + NT PTSR+R LT T Sbjct: 382 NGGAHHNSQEQSDRLIGSNSPT-----NKPHNGNNT----DPTSRFRQLLTNT 425
>FA21C_HUMAN (Q9Y4E1) Protein FAM21C| Length = 1320 Score = 31.2 bits (69), Expect = 3.3 Identities = 35/145 (24%), Positives = 57/145 (39%), Gaps = 16/145 (11%) Frame = -1 Query: 542 EVKPQGVSKGLVAKRMLSAMQARGLLPDFILCIGDDRSDEDMFEVITTAVDGPYLNPEAK 363 E + V +G +A+ A+ G P G+D ++E + GP + Sbjct: 1034 EAEDMSVPRGPIAQWADGAISPNGHRPQLRAASGEDSTEEALAAAAAPWEGGPVPGVDTS 1093 Query: 362 VFACTVGRKPSKAKYYLDDTADI---------VRLIQALANVSDE-----VQGGPPSYFF 225 FA ++G +A + D+ DI V + A +++ V G S F Sbjct: 1094 PFAKSLGHSRGEADLF--DSGDIFSTGTGSQSVERTKPKAKIAENPANPPVGGKAKSPMF 1151 Query: 224 ACAG--TSTDDRALN*GGKPAKKTS 156 G +S DD + KPAKKT+ Sbjct: 1152 PALGEASSDDDLFQSAKPKPAKKTN 1176
>THIC_THETN (Q8R9P1) Thiamine biosynthesis protein thiC| Length = 432 Score = 30.4 bits (67), Expect = 5.6 Identities = 15/33 (45%), Positives = 21/33 (63%) Frame = -1 Query: 599 LESVLSNEPVSVKADVNHVEVKPQGVSKGLVAK 501 LE V E V + A+VNH+ + P+G+ KGL K Sbjct: 31 LEEVKKGEIV-IPANVNHINLVPKGIGKGLSTK 62
>CAPP_THET8 (Q5SKL7) Phosphoenolpyruvate carboxylase (EC 4.1.1.31) (PEPCase)| (PEPC) Length = 858 Score = 30.0 bits (66), Expect = 7.3 Identities = 22/55 (40%), Positives = 27/55 (49%) Frame = -1 Query: 281 QALANVSDEVQGGPPSYFFACAGTSTDDRALN*GGKPAKKTSTSTAPA*IRPRFR 117 +AL+ V +EV G P YFF GTST GG PA + S P + R R Sbjct: 547 EALSRVGEEV--GLPVYFFHGRGTSTAR-----GGGPAGRAIASLPPRSVGRRIR 594
>CAPP_THET2 (Q72L05) Phosphoenolpyruvate carboxylase (EC 4.1.1.31) (PEPCase)| (PEPC) Length = 858 Score = 30.0 bits (66), Expect = 7.3 Identities = 22/55 (40%), Positives = 27/55 (49%) Frame = -1 Query: 281 QALANVSDEVQGGPPSYFFACAGTSTDDRALN*GGKPAKKTSTSTAPA*IRPRFR 117 +AL+ V +EV G P YFF GTST GG PA + S P + R R Sbjct: 547 EALSRVGEEV--GLPVYFFHGRGTSTAR-----GGGPAGRAIASLPPRSVGRRIR 594
>FA21C_PONPY (Q5RDC1) Protein FAM21C| Length = 1340 Score = 30.0 bits (66), Expect = 7.3 Identities = 35/147 (23%), Positives = 56/147 (38%), Gaps = 18/147 (12%) Frame = -1 Query: 542 EVKPQGVSKGLVAKRMLSAMQARGLLPDFILCIGDDRSDEDMFEVITTAVDGPYLNPEAK 363 E + V +G +A+ + G P G+D ++E + GP + Sbjct: 1054 EAEDMSVPRGPIAQWADGTISPNGHRPQLRAASGEDSTEEALAAAAAPWEGGPVPGVDRS 1113 Query: 362 VFACTVGRKPSKAKYYLDDTADI----------------VRLIQALANVSDEVQGGPPSY 231 FA ++G +A + D+ DI ++ + LAN V G S Sbjct: 1114 PFAKSLGHSRGEADLF--DSGDIFSTGTGSQSEERTKPKAKIAENLAN--PPVGGKAKSP 1169 Query: 230 FFACAG--TSTDDRALN*GGKPAKKTS 156 F G +S DD + KPAKKT+ Sbjct: 1170 MFPALGEASSDDDLFQSAKPKPAKKTN 1196 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 85,043,122 Number of Sequences: 219361 Number of extensions: 1618558 Number of successful extensions: 4977 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 4861 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 4973 length of database: 80,573,946 effective HSP length: 109 effective length of database: 56,663,597 effective search space used: 7252940416 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
