ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | rbasd25m22 |
|---|---|
| Clone Library Name | barley_pub |
>PGLR_ACTCH (P35336) Polygalacturonase precursor (EC 3.2.1.15) (PG) (Pectinase)| Length = 467 Score = 41.6 bits (96), Expect = 0.002 Identities = 20/53 (37%), Positives = 29/53 (54%) Frame = -2 Query: 632 ISEVRAEGLRIVNSLHGIRIKTAPGRGGYVRNVYIADVSMHNVSMAIRITGNY 474 +S+V G ++ + +G+RIKT G G N+ +V MHNV I I NY Sbjct: 322 VSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNY 374
>PGLR_AGRTU (P27644) Polygalacturonase (EC 3.2.1.15) (Pectinase) (PGL)| Length = 312 Score = 40.0 bits (92), Expect = 0.006 Identities = 36/129 (27%), Positives = 56/129 (43%), Gaps = 10/129 (7%) Frame = -2 Query: 644 MSGGISEVRAEGLRIVNSLHGIRIKT-APGRGGYVRNVYIADVSMHNVSMAIRITGNY-- 474 MSGG+ +V E ++ + G+R+KT A GG V N+ + V + V A+ +Y Sbjct: 139 MSGGVHDVTVEDCDMIGTDRGLRLKTGARSGGGMVGNITMRRVLLDGVQTALSANAHYHC 198 Query: 473 --GEHPDSKVLRATTSVAYASPM--SLSAYDPDIHGIAH---VSKGIPTP*PQSRASNSK 315 H D R V +P ++ D +I +AH V G+P + S + Sbjct: 199 DADGHDDWVQSRNPAPVNDGTPFVDGITVEDVEIRNLAHAAGVFLGLPDVPSATSLSATS 258 Query: 314 QIVKRHQSA 288 IV SA Sbjct: 259 PIVSHDPSA 267
>PGLR_GOSHI (Q39786) Polygalacturonase precursor (EC 3.2.1.15) (PG) (Pectinase)| Length = 407 Score = 39.3 bits (90), Expect = 0.010 Identities = 20/54 (37%), Positives = 30/54 (55%), Gaps = 1/54 (1%) Frame = -2 Query: 632 ISEVRAEGLRIVNSLHGIRIKTAPGR-GGYVRNVYIADVSMHNVSMAIRITGNY 474 + ++ I N+ +G RIKT PG GG V ++ D++M+NVS I I Y Sbjct: 260 VEGIKISNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQY 313
>PGLR_GOSBA (Q39766) Polygalacturonase precursor (EC 3.2.1.15) (PG) (Pectinase)| Length = 407 Score = 39.3 bits (90), Expect = 0.010 Identities = 20/54 (37%), Positives = 30/54 (55%), Gaps = 1/54 (1%) Frame = -2 Query: 632 ISEVRAEGLRIVNSLHGIRIKTAPGR-GGYVRNVYIADVSMHNVSMAIRITGNY 474 + ++ I N+ +G RIKT PG GG V ++ D++M+NVS I I Y Sbjct: 260 VEGIKISNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQY 313
>PGLR_PERAE (Q02096) Polygalacturonase precursor (EC 3.2.1.15) (PG) (Pectinase)| Length = 462 Score = 38.9 bits (89), Expect = 0.013 Identities = 24/82 (29%), Positives = 42/82 (51%), Gaps = 4/82 (4%) Frame = -2 Query: 632 ISEVRAEGLRIVNSLHGIRIKTAPGRGGYVRNVYIADVSMHNVSMAIRITGNYGEH---- 465 +S V +G + ++ +G+RIKT G G +N+ ++ MHNV+ I I Y + Sbjct: 317 VSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYCDSKDPC 376 Query: 464 PDSKVLRATTSVAYASPMSLSA 399 P+ + ++VAY + SA Sbjct: 377 PEQESAVKVSNVAYMNIRGTSA 398
>PGLR_MALDO (P48978) Polygalacturonase precursor (EC 3.2.1.15) (PG) (Pectinase)| Length = 460 Score = 37.7 bits (86), Expect = 0.029 Identities = 19/56 (33%), Positives = 31/56 (55%) Frame = -2 Query: 632 ISEVRAEGLRIVNSLHGIRIKTAPGRGGYVRNVYIADVSMHNVSMAIRITGNYGEH 465 +S V G ++ + +G+RIKT G G N+ +V M++V+ I I NY +H Sbjct: 331 VSGVFVNGAKLSGTSNGLRIKTWKGGSGSATNIVFQNVQMNDVTNPIIIDQNYCDH 386
>Y750_METJA (Q58160) Hypothetical protein MJ0750| Length = 238 Score = 37.0 bits (84), Expect = 0.050 Identities = 24/79 (30%), Positives = 37/79 (46%), Gaps = 3/79 (3%) Frame = -2 Query: 242 IISLVATYY*ILYCICSRCNVGRYRCLLFVAKGLCIMGCVLLVMY---ISFLHQFCLDCS 72 I+SLV TYY +Y C C +G L L I+ L + + ++F FC + Sbjct: 110 ILSLVLTYYLSIYAFCQVCPIGFLTNLYGTVISLIILIFFLSLSFFVPMAFCRYFCPLGA 169 Query: 71 FLHVYSXEPGLXLLPXSIC 15 FL ++S +P L + C Sbjct: 170 FLSIFSIKPFFQLKTNNNC 188
>PGLR_LYCES (P05117) Polygalacturonase-2 precursor (EC 3.2.1.15) (PG) (PG-2A)| (PG-2B) (Pectinase) Length = 457 Score = 35.8 bits (81), Expect = 0.11 Identities = 18/53 (33%), Positives = 27/53 (50%) Frame = -2 Query: 632 ISEVRAEGLRIVNSLHGIRIKTAPGRGGYVRNVYIADVSMHNVSMAIRITGNY 474 +S V +I+ + +G+RIKT G G N+ +V M +V I I NY Sbjct: 309 VSNVTVNEAKIIGAENGVRIKTWQGGSGQASNIKFLNVEMQDVKYPIIIDQNY 361
>PGLR_ASPPA (P49575) Polygalacturonase precursor (EC 3.2.1.15) (Pectinase)| (PGL) Length = 363 Score = 35.4 bits (80), Expect = 0.15 Identities = 15/44 (34%), Positives = 26/44 (59%) Frame = -2 Query: 632 ISEVRAEGLRIVNSLHGIRIKTAPGRGGYVRNVYIADVSMHNVS 501 + +V ++VNS +G+RIKT G G V NV D+++ ++ Sbjct: 239 VEDVTISNSKVVNSQNGVRIKTVYGATGTVSNVKFEDITLSGIT 282
>PGLR2_ARATH (P49063) Exopolygalacturonase clone GBGA483 precursor (EC 3.2.1.67)| (ExoPG) (Pectinase) (Galacturan 1,4-alpha-galacturonidase) Length = 444 Score = 34.7 bits (78), Expect = 0.25 Identities = 18/44 (40%), Positives = 26/44 (59%), Gaps = 1/44 (2%) Frame = -2 Query: 602 IVNSLHGIRIKTAPGR-GGYVRNVYIADVSMHNVSMAIRITGNY 474 I N+ +G+RIKT PG G N+ D++M NVS+ + I Y Sbjct: 310 IKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEY 353
>PGLR_ASPOR (P35335) Polygalacturonase precursor (EC 3.2.1.15) (PG) (Pectinase)| Length = 363 Score = 32.7 bits (73), Expect = 0.94 Identities = 14/44 (31%), Positives = 25/44 (56%) Frame = -2 Query: 632 ISEVRAEGLRIVNSLHGIRIKTAPGRGGYVRNVYIADVSMHNVS 501 + +V ++VNS +G+RIKT G V NV D+++ ++ Sbjct: 239 VEDVTISNSKVVNSQNGVRIKTVYDATGTVSNVKFEDITLSGIT 282
>PGLRA_ASPFL (P41749) Polygalacturonase A precursor (EC 3.2.1.15) (Pectinase)| (PGL) (P2C) Length = 363 Score = 32.7 bits (73), Expect = 0.94 Identities = 14/44 (31%), Positives = 25/44 (56%) Frame = -2 Query: 632 ISEVRAEGLRIVNSLHGIRIKTAPGRGGYVRNVYIADVSMHNVS 501 + +V ++VNS +G+RIKT G V NV D+++ ++ Sbjct: 239 VEDVTISNSKVVNSQNGVRIKTVYDATGTVSNVKFEDITLSGIT 282
>PGLR_PENEN (O59925) Polygalacturonase precursor (EC 3.2.1.15) (PG) (Pectinase)| Length = 378 Score = 32.3 bits (72), Expect = 1.2 Identities = 15/54 (27%), Positives = 27/54 (50%), Gaps = 1/54 (1%) Frame = -2 Query: 632 ISEVRAEGLRIVNSLHGIRIKTAPGRGGYVRNVYIADVSMHNV-SMAIRITGNY 474 + V ++VNS +G+R+K G G ++ V D+++ + S I I Y Sbjct: 255 VETVHISSTQVVNSQNGVRVKAVSGATGTIKGVTFQDITLSGITSQGITIRQEY 308
>PGLR1_SCLSC (Q12708) Endo-polygalacturonase precursor (EC 3.2.1.15) (PG)| (Pectinase) Length = 380 Score = 32.3 bits (72), Expect = 1.2 Identities = 15/44 (34%), Positives = 25/44 (56%) Frame = -2 Query: 632 ISEVRAEGLRIVNSLHGIRIKTAPGRGGYVRNVYIADVSMHNVS 501 +S+V E + NS +G+RIKT G G V V D+++ ++ Sbjct: 259 VSDVIIESSTVKNSANGVRIKTVLGATGSVSGVTYKDITLSGIT 302
>PGLR_YEAST (P47180) Polygalacturonase precursor (EC 3.2.1.15) (PG) (Pectinase)| (PGase SM) Length = 361 Score = 32.0 bits (71), Expect = 1.6 Identities = 20/49 (40%), Positives = 28/49 (57%), Gaps = 1/49 (2%) Frame = -2 Query: 617 AEGLRIVNSLHGIRIKTAPGRGGYVRNV-YIADVSMHNVSMAIRITGNY 474 AE ++NS +G+RIKT G G V NV +I++ S I I G+Y Sbjct: 242 AENNHVINSDNGLRIKTVEGATGTVTNVNFISNKISGIKSYGIVIEGDY 290
>ATP6_TRYBB (P24499) ATP synthase a chain (EC 3.6.3.14) (ATPase protein 6)| Length = 229 Score = 31.6 bits (70), Expect = 2.1 Identities = 12/35 (34%), Positives = 22/35 (62%) Frame = -2 Query: 173 YRCLLFVAKGLCIMGCVLLVMYISFLHQFCLDCSF 69 Y C+ ++ L ++ C++ ++YI+FL FC C F Sbjct: 73 YYCITYL--NLYLLFCIVFLLYIAFLFLFCFLCDF 105
>PGLR1_COLLN (Q00446) Endopolygalacturonase 1 precursor (EC 3.2.1.15)| (Pectinase) (Clpg1) Length = 363 Score = 31.6 bits (70), Expect = 2.1 Identities = 13/44 (29%), Positives = 26/44 (59%) Frame = -2 Query: 632 ISEVRAEGLRIVNSLHGIRIKTAPGRGGYVRNVYIADVSMHNVS 501 + V +I+NS +G+RIKT G G V ++ + +++ N++ Sbjct: 239 VKSVSITNSKIINSDNGVRIKTVAGATGPVSDITYSGITLSNIA 282
>PGLR_GIBFU (Q07181) Polygalacturonase precursor (EC 3.2.1.15) (PG) (Pectinase)| (FmPG) Length = 373 Score = 31.6 bits (70), Expect = 2.1 Identities = 17/51 (33%), Positives = 30/51 (58%), Gaps = 5/51 (9%) Frame = -2 Query: 638 GGISEVRAEGLR-----IVNSLHGIRIKTAPGRGGYVRNVYIADVSMHNVS 501 GG S+ +G++ +VNS +G RIK+ G G + NV ++++ N+S Sbjct: 242 GGKSDNVVDGVQFLSSQVVNSQNGCRIKSNSGATGTINNVTYQNIALTNIS 292
>PGLR_COCCA (P26215) Polygalacturonase precursor (EC 3.2.1.15) (PG) (Pectinase)| Length = 364 Score = 31.2 bits (69), Expect = 2.7 Identities = 14/44 (31%), Positives = 25/44 (56%) Frame = -2 Query: 632 ISEVRAEGLRIVNSLHGIRIKTAPGRGGYVRNVYIADVSMHNVS 501 + V G I NS +G+RIKT G G V ++ ++++ N++ Sbjct: 240 VKGVVVSGTTIANSDNGVRIKTISGATGSVSDITYENITLKNIA 283
>PGLR2_JUNAS (Q9FY19) Polygalacturonase precursor (PG) (EC 3.2.1.15) (Pectinase)| (Major pollen allergen Jun a 2) Length = 507 Score = 31.2 bits (69), Expect = 2.7 Identities = 16/53 (30%), Positives = 27/53 (50%) Frame = -2 Query: 632 ISEVRAEGLRIVNSLHGIRIKTAPGRGGYVRNVYIADVSMHNVSMAIRITGNY 474 +S V +G + +++ +G+RIKT G G ++ +V M N I I Y Sbjct: 295 VSFVHLDGAKFIDTQNGLRIKTWQGGSGLASHITYENVEMINAENPILINQFY 347
>PGLR2_CRYJA (P43212) Polygalacturonase precursor (EC 3.2.1.15) (PG) (Pectinase)| (Major pollen allergen Cry j 2) (Cry j II) Length = 514 Score = 30.8 bits (68), Expect = 3.6 Identities = 16/53 (30%), Positives = 26/53 (49%) Frame = -2 Query: 632 ISEVRAEGLRIVNSLHGIRIKTAPGRGGYVRNVYIADVSMHNVSMAIRITGNY 474 +S V G + +++ +G+RIKT G G ++ +V M N I I Y Sbjct: 294 VSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFY 346
>PEHX_ERWCH (P15922) Exo-poly-alpha-D-galacturonosidase precursor (EC 3.2.1.82)| (Exo-PG) Length = 602 Score = 30.8 bits (68), Expect = 3.6 Identities = 18/58 (31%), Positives = 32/58 (55%), Gaps = 1/58 (1%) Frame = -2 Query: 635 GISEVRAEGLRIVNSLHGIRIKTAPGRGGYVRNVYIADVSMHNVS-MAIRITGNYGEH 465 GI +V AE I + G+R K+AP GG + + +M N++ A+ +T +Y ++ Sbjct: 440 GIVDVLAENNVITQNDVGLRAKSAPAIGGGAHGIVFRNSAMKNLAKQAVIVTLSYADN 497
>PGLR2_PENOL (Q9Y833) Polygalacturonase 2 precursor (EC 3.2.1.15) (PG 2)| (Pectinase 2) Length = 380 Score = 30.0 bits (66), Expect = 6.1 Identities = 14/44 (31%), Positives = 26/44 (59%) Frame = -2 Query: 632 ISEVRAEGLRIVNSLHGIRIKTAPGRGGYVRNVYIADVSMHNVS 501 ++ V E +I +S +G+RIKT G V+NV D+++ ++ Sbjct: 256 VANVIIENSQIQDSTNGVRIKTVYEATGSVKNVTYKDITLSGIT 299
>CRF1_ASPFU (Q8J0P4) Probable glycosidase crf1 precursor (EC 3.2.-.-) (Crh-like| protein) (Allergen Asp f 9) Length = 395 Score = 30.0 bits (66), Expect = 6.1 Identities = 21/60 (35%), Positives = 29/60 (48%) Frame = -2 Query: 497 AIRITGNYGEHPDSKVLRATTSVAYASPMSLSAYDPDIHGIAHVSKGIPTP*PQSRASNS 318 +I+ G+ S V +TTS A +S S S+ P +A +K PTP S SNS Sbjct: 262 SIKFDGSVDISSSSSVTSSTTSTA-SSASSTSSKTPSTSTLATSTKATPTPSGTSSGSNS 320
>GPX1_PEA (O24296) Phospholipid hydroperoxide glutathione peroxidase,| chloroplast precursor (EC 1.11.1.12) (PHGPx) Length = 236 Score = 29.6 bits (65), Expect = 8.0 Identities = 14/49 (28%), Positives = 28/49 (57%), Gaps = 4/49 (8%) Frame = +1 Query: 484 VILMAIETLCMLTSAIYTFLTYP----PRPGAVLILIPCNEFTMRRPSA 618 ++++ + + C LTS+ YT L++ G ++ PCN+F M+ P + Sbjct: 102 LLIVNVASRCGLTSSNYTELSHLYENFKNKGLEVLAFPCNQFGMQEPGS 150
>PGLRB_ASPFL (P41750) Polygalacturonase B precursor (EC 3.2.1.15) (Pectinase)| (PGL) (P1/P3) Length = 366 Score = 29.6 bits (65), Expect = 8.0 Identities = 14/43 (32%), Positives = 24/43 (55%) Frame = -2 Query: 632 ISEVRAEGLRIVNSLHGIRIKTAPGRGGYVRNVYIADVSMHNV 504 + V+ +V+S +GIRIKT G G V V ++++ N+ Sbjct: 242 VKNVKVADSTVVDSDNGIRIKTISGATGSVSGVTYENITLKNI 284
>PGLR1_ASPAC (O74213) Polygalacturonase 1 precursor (EC 3.2.1.15) (PG-1)| (Pectinase 1) Length = 378 Score = 29.6 bits (65), Expect = 8.0 Identities = 16/46 (34%), Positives = 28/46 (60%), Gaps = 1/46 (2%) Frame = -2 Query: 602 IVNSLHGIRIKTAPGRGGYVRNVYIADVSMHNVS-MAIRITGNYGE 468 I+NS +G+RIKT G V +V D+++ +++ I + NYG+ Sbjct: 266 IINSDNGVRIKTNIDTTGSVSDVTYKDITLTSIAKYGIVVQQNYGD 311
>IDNT_ECOLI (P39344) Gnt-II system L-idonate transporter (L-Ido transporter)| Length = 439 Score = 29.6 bits (65), Expect = 8.0 Identities = 21/60 (35%), Positives = 30/60 (50%), Gaps = 5/60 (8%) Frame = +1 Query: 481 PVILMAIETLCML----TSAIYTFLTYPPRPG-AVLILIPCNEFTMRRPSARTSDMPPDI 645 PVILMAI +C + T+ + F + P A+ I I FT+ R + RT + DI Sbjct: 232 PVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFTLGRRNGRTIEQIMDI 291
>CVP1_PIMHY (Q8T0W5) Cysteine-rich venom protein 1 precursor| Length = 85 Score = 29.6 bits (65), Expect = 8.0 Identities = 19/56 (33%), Positives = 22/56 (39%) Frame = -1 Query: 372 SRIQGYSNSVTPESCEQLKTDCEETPICYNGGSSLAFRSRASIHNKPSGNILLNSL 205 +RI P +CE DC ETP C G F I N GN + SL Sbjct: 32 NRIYKTCGPACPPTCEDPDPDCNETPQCKAG----CFCIPGLIENMKGGNCISPSL 83 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 93,631,497 Number of Sequences: 219361 Number of extensions: 1959019 Number of successful extensions: 4787 Number of sequences better than 10.0: 29 Number of HSP's better than 10.0 without gapping: 4660 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 4784 length of database: 80,573,946 effective HSP length: 108 effective length of database: 56,882,958 effective search space used: 6029593548 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
