ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd25f13
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1NEP2_NEPGR (Q766C2) Aspartic proteinase nepenthesin-2 precursor ... 65 1e-10
2NEP1_NEPGR (Q766C3) Aspartic proteinase nepenthesin-1 precursor ... 59 6e-09
3NEP2_NEPDI (P69477) Aspartic proteinase nepenthesin-2 (EC 3.4.23... 41 0.002
4SGS3_DROME (P02840) Salivary glue protein Sgs-3 precursor 33 0.60
5AROA_CORGL (Q9Z470) 3-phosphoshikimate 1-carboxyvinyltransferase... 33 0.60
6YHU3_YEAST (P38844) Protein YHR143W precursor 29 8.6

>NEP2_NEPGR (Q766C2) Aspartic proteinase nepenthesin-2 precursor (EC 3.4.23.-)|
           (Nepenthesin-II)
          Length = 438

 Score = 64.7 bits (156), Expect = 1e-10
 Identities = 43/129 (33%), Positives = 61/129 (47%), Gaps = 6/129 (4%)
 Frame = -2

Query: 513 SAGSVMDSGTIITRLPPTAYTALKTAFKDGMKQYPTAPARSILDTCFDF-SGQDNVTIPT 337
           + G ++DSGT +T LP  AY A+  AF D +       + S L TCF   S    V +P 
Sbjct: 309 TGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTVQVPE 368

Query: 336 VALVFDGGKVVDLDANGIIFGS-----CLAFXXXXXXXXXGIIGNVQQRTLEVLYDVGQS 172
           +++ FDGG V++L    I+        CLA           I GN+QQ+  +VLYD+   
Sbjct: 369 ISMQFDGG-VLNLGEQNILISPAEGVICLAM-GSSSQLGISIFGNIQQQETQVLYDLQNL 426

Query: 171 VFGFKSNAC 145
              F    C
Sbjct: 427 AVSFVPTQC 435



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>NEP1_NEPGR (Q766C3) Aspartic proteinase nepenthesin-1 precursor (EC 3.4.23.-)|
           (Nepenthesin-I)
          Length = 437

 Score = 59.3 bits (142), Expect = 6e-09
 Identities = 42/128 (32%), Positives = 58/128 (45%), Gaps = 5/128 (3%)
 Frame = -2

Query: 513 SAGSVMDSGTIITRLPPTAYTALKTAFKDGMKQYPTAPARSILDTCFDF-SGQDNVTIPT 337
           + G ++DSGT +T     AY +++  F   +       + S  D CF   S   N+ IPT
Sbjct: 309 TGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPT 368

Query: 336 VALVFDGGKVVDLDANGIIFGS----CLAFXXXXXXXXXGIIGNVQQRTLEVLYDVGQSV 169
             + FDGG +     N  I  S    CLA           I GN+QQ+ + V+YD G SV
Sbjct: 369 FVMHFDGGDLELPSENYFISPSNGLICLAMGSSSQGMS--IFGNIQQQNMLVVYDTGNSV 426

Query: 168 FGFKSNAC 145
             F S  C
Sbjct: 427 VSFASAQC 434



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>NEP2_NEPDI (P69477) Aspartic proteinase nepenthesin-2 (EC 3.4.23.-)|
           (Nepenthesin-II) (Fragments)
          Length = 178

 Score = 41.2 bits (95), Expect = 0.002
 Identities = 27/75 (36%), Positives = 39/75 (52%), Gaps = 1/75 (1%)
 Frame = -2

Query: 498 MDSGTIITRLPPTAYTALKTAFKDGMKQYPTAPARSILDTCF-DFSGQDNVTIPTVALVF 322
           +DSGT +T LP  AY A+  AF D +       + S L TCF + S    V +P +++  
Sbjct: 97  IDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQEPSDGSTVQVPEISMQ- 155

Query: 321 DGGKVVDLDANGIIF 277
           DGG + DL    + F
Sbjct: 156 DGGVLNDLQNLAVSF 170



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>SGS3_DROME (P02840) Salivary glue protein Sgs-3 precursor|
          Length = 307

 Score = 32.7 bits (73), Expect = 0.60
 Identities = 21/72 (29%), Positives = 30/72 (41%)
 Frame = +2

Query: 179 PTS*STSRVLCCTXXXXXXXXXXXXXXNARQLPNMIPLASRSTTLPPSNTKATVGMVTLS 358
           PT  ST++  C T                 QLP   P  +++TT  P+ TKAT    T +
Sbjct: 58  PTQQSTTQPPCTTSKPTTPKQTTT------QLPCTTPTTTKATTTKPTTTKATTTKATTT 111

Query: 359 WPLKSKHVSRML 394
            P  +K  +  L
Sbjct: 112 KPTTTKQTTTQL 123



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>AROA_CORGL (Q9Z470) 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19)|
           (5-enolpyruvylshikimate-3-phosphate synthase) (EPSP
           synthase) (EPSPS)
          Length = 430

 Score = 32.7 bits (73), Expect = 0.60
 Identities = 31/82 (37%), Positives = 40/82 (48%), Gaps = 6/82 (7%)
 Frame = -2

Query: 513 SAGSVMD--SGTIITRLPPTAYTALKTAFKDGMKQYPTAPARSILDTCFDFSGQ-DNVTI 343
           SAGSV    +GT++  LPP A  A      DG  Q    P  SILD       + DN  +
Sbjct: 91  SAGSVECGLAGTVMRFLPPVAAFADGPVHFDGDPQARVRPMTSILDALRSLGVEVDNNNL 150

Query: 342 P---TVALVFDGGKVVDLDANG 286
           P       V +GG VV++DA+G
Sbjct: 151 PFTVNAGEVPEGG-VVEIDASG 171



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>YHU3_YEAST (P38844) Protein YHR143W precursor|
          Length = 325

 Score = 28.9 bits (63), Expect = 8.6
 Identities = 13/45 (28%), Positives = 25/45 (55%)
 Frame = +2

Query: 290 LASRSTTLPPSNTKATVGMVTLSWPLKSKHVSRMLLAGAVGYCFM 424
           +A  ++TL PS++  T    T S PL+S     + ++ + G C++
Sbjct: 243 IARTTSTLVPSSSVDTTSRATTSMPLESSSTQSISVSSSDGTCYV 287


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52,248,410
Number of Sequences: 219361
Number of extensions: 867706
Number of successful extensions: 2807
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 2759
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2804
length of database: 80,573,946
effective HSP length: 104
effective length of database: 57,760,402
effective search space used: 3812186532
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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