ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd24i22
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1EBM_ARATH (Q75W54) Mannosylglycoprotein endo-beta-mannosidase (E... 98 1e-20
2EBM_LILLO (Q5H7P5) Mannosylglycoprotein endo-beta-mannosidase (E... 96 7e-20
3PO2F1_CHICK (P15143) POU domain, class 2, transcription factor 1... 30 3.7
4PROA_PSEPF (Q3K693) Gamma-glutamyl phosphate reductase (GPR) (EC... 30 4.8
5GSPN_PECCC (P31710) General secretion pathway protein N (Pectic ... 29 6.3
6PO2F1_PIG (Q29076) POU domain, class 2, transcription factor 1 (... 29 8.2
7PO2F1_RAT (P31503) POU domain, class 2, transcription factor 1 (... 29 8.2

>EBM_ARATH (Q75W54) Mannosylglycoprotein endo-beta-mannosidase (EC 3.2.1.152)|
            (Endo-beta-mannosidase) (AtEBM) [Contains:
            Mannosylglycoprotein endo-beta-mannosidase 31 kDa
            subunit; Mannosylglycoprotein endo-beta-mannosidase 28
            kDa subunit; Mannosylglycopro
          Length = 943

 Score = 97.8 bits (242), Expect = 1e-20
 Identities = 49/77 (63%), Positives = 58/77 (75%), Gaps = 2/77 (2%)
 Frame = -2

Query: 477  LRVNGTDSGVAFFLHFSVHTSESSIGENYRDTRILPVHYSDNYFSLTPGEKMTTDISFEA 298
            + + G+DSGVAFFL FSVH +E+      +DTRILPVHYSDNYFSL PGE M+  ISF A
Sbjct: 863  VEMKGSDSGVAFFLRFSVHNAETE----KQDTRILPVHYSDNYFSLVPGESMSFKISFAA 918

Query: 297  PEGSK--PRVILRGWNY 253
            P G K  PRV+L+GWNY
Sbjct: 919  PTGMKKSPRVMLQGWNY 935



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>EBM_LILLO (Q5H7P5) Mannosylglycoprotein endo-beta-mannosidase (EC 3.2.1.152)|
            (Endo-beta-mannosidase) [Contains: Mannosylglycoprotein
            endo-beta-mannosidase 31 kDa subunit;
            Mannosylglycoprotein endo-beta-mannosidase 28 kDa
            subunit; Mannosylglycoprotein end
          Length = 952

 Score = 95.5 bits (236), Expect = 7e-20
 Identities = 48/79 (60%), Positives = 50/79 (63%)
 Frame = -2

Query: 477  LRVNGTDSGVAFFLHFSVHTSESSIGENYRDTRILPVHYSDNYFSLTPGEKMTTDISFEA 298
            +   GT SGVAFFLHFSVH  +    E   D RILPVHYSDNYFSL PGE     ISFE 
Sbjct: 871  VETKGTGSGVAFFLHFSVHAVKKDENE-IEDLRILPVHYSDNYFSLVPGETTNISISFEV 929

Query: 297  PEGSKPRVILRGWNYHLNH 241
            P G  PRV LRGWN    H
Sbjct: 930  PHGVTPRVSLRGWNCSEEH 948



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>PO2F1_CHICK (P15143) POU domain, class 2, transcription factor 1|
           (Octamer-binding transcription factor 1) (Oct-1) (OTF-1)
           (NF-A1)
          Length = 739

 Score = 30.0 bits (66), Expect = 3.7
 Identities = 20/58 (34%), Positives = 27/58 (46%)
 Frame = -3

Query: 395 ITGTLESFRYTTQTTIFRSPPGRR*QLTSPSRHPKDPSPGSF*EAGTII*TMLSQCES 222
           +TGT E+    T T I  +PP     +TSPS  P   +  S  EA +   T  +Q  S
Sbjct: 490 VTGTTETTSNNTATVISTAPPASS-AVTSPSLSPSPSASASISEASSASETSTTQTTS 546



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>PROA_PSEPF (Q3K693) Gamma-glutamyl phosphate reductase (GPR) (EC 1.2.1.41)|
           (Glutamate-5-semialdehyde dehydrogenase)
           (Glutamyl-gamma-semialdehyde dehydrogenase) (GSA
           dehydrogenase)
          Length = 423

 Score = 29.6 bits (65), Expect = 4.8
 Identities = 12/31 (38%), Positives = 17/31 (54%)
 Frame = -2

Query: 471 VNGTDSGVAFFLHFSVHTSESSIGENYRDTR 379
           V+G D  +    HF  H ++S + EN  DTR
Sbjct: 325 VDGLDQAIEHINHFGSHHTDSIVSENLADTR 355



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>GSPN_PECCC (P31710) General secretion pathway protein N (Pectic enzymes|
           secretion protein OUTN)
          Length = 248

 Score = 29.3 bits (64), Expect = 6.3
 Identities = 15/46 (32%), Positives = 24/46 (52%)
 Frame = -2

Query: 396 NYRDTRILPVHYSDNYFSLTPGEKMTTDISFEAPEGSKPRVILRGW 259
           ++R   +  VH++  +    P      DI+F+ PEG   R I+RGW
Sbjct: 57  SWRTLTLDDVHWNITFSDFMPA----LDIAFKNPEGIAGRGIIRGW 98



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>PO2F1_PIG (Q29076) POU domain, class 2, transcription factor 1|
           (Octamer-binding transcription factor 1) (Oct-1) (OTF-1)
           (NF-A1)
          Length = 745

 Score = 28.9 bits (63), Expect = 8.2
 Identities = 19/58 (32%), Positives = 27/58 (46%)
 Frame = -3

Query: 395 ITGTLESFRYTTQTTIFRSPPGRR*QLTSPSRHPKDPSPGSF*EAGTII*TMLSQCES 222
           +TGT ++    T T I  +PP     +TSPS  P   + GS  E  +   T  +Q  S
Sbjct: 496 VTGTTDTTSNNTATVISTAPPASS-AVTSPSLSPSPSASGSTSEVSSASETSTTQTTS 552



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>PO2F1_RAT (P31503) POU domain, class 2, transcription factor 1|
           (Octamer-binding transcription factor 1) (Oct-1) (OTF-1)
           (NF-A1) (Fragment)
          Length = 632

 Score = 28.9 bits (63), Expect = 8.2
 Identities = 20/58 (34%), Positives = 27/58 (46%)
 Frame = -3

Query: 395 ITGTLESFRYTTQTTIFRSPPGRR*QLTSPSRHPKDPSPGSF*EAGTII*TMLSQCES 222
           +TGT +S    T T I  +PP     +TSPS  P   +  S  EA +   T  +Q  S
Sbjct: 383 LTGTTDSTSNNTATVISTAPPASS-AVTSPSLSPSPSASASTSEASSASETSTTQTTS 439


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 72,711,884
Number of Sequences: 219361
Number of extensions: 1516287
Number of successful extensions: 3174
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 3028
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3171
length of database: 80,573,946
effective HSP length: 104
effective length of database: 57,760,402
effective search space used: 3638905326
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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