ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd23o05
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1TRK1_SACBA (P28569) High-affinity potassium transport protein 37 0.042
2PHLB_MYCTU (P95246) Phospholipase C 2 precursor (EC 3.1.4.3) 34 0.36
3PHLC_MYCTU (P95245) Phospholipase C 3 precursor (EC 3.1.4.3) 33 0.47
4NUPL_HUMAN (P52594) Nucleoporin-like protein RIP (HIV-1 Rev-bind... 32 1.4
5PHLN_BURPS (Q9RGS8) Non-hemolytic phospholipase C precursor (EC ... 32 1.4
6UBE3A_MOUSE (O08759) Ubiquitin-protein ligase E3A (EC 6.3.2.-) (... 32 1.8
7PDP_BACSU (P39142) Pyrimidine-nucleoside phosphorylase (EC 2.4.2... 31 2.3
8NUPL_BOVIN (Q2TA45) Nucleoporin-like protein RIP (HIV-1 Rev-bind... 31 3.0
9RPO3B_TOBAC (Q8L6J1) DNA-directed RNA polymerase 3B, chloroplast... 31 3.0
10PHLA_MYCTU (Q04001) Phospholipase C 1 precursor (EC 3.1.4.3) (MT... 31 3.0
11MMP9_MOUSE (P41245) Matrix metalloproteinase-9 precursor (EC 3.4... 30 4.0
12KITH_HAEDU (Q7VLH2) Thymidine kinase (EC 2.7.1.21) 30 4.0
13RPOT3_NICSY (P69242) DNA-directed RNA polymerase 3, chloroplast ... 30 5.2
14RPO3A_TOBAC (P69243) DNA-directed RNA polymerase 3A, chloroplast... 30 5.2
15GSH1_PHOLL (Q7N7A4) Glutamate--cysteine ligase (EC 6.3.2.2) (Gam... 30 6.7
16UCRIC_ANASP (P70758) Cytochrome b6-f complex iron-sulfur subunit... 30 6.7
17WASIP_RAT (Q6IN36) Wiskott-Aldrich syndrome protein-interacting ... 29 8.8
18SCNNA_BOVIN (P55270) Amiloride-sensitive sodium channel alpha-su... 29 8.8
19MYH6_HUMAN (P13533) Myosin heavy chain, cardiac muscle alpha iso... 29 8.8
20GLMM_DESPS (Q6AMQ5) Phosphoglucosamine mutase (EC 5.4.2.10) 29 8.8
21ARGD_BACAN (Q81M98) Acetylornithine aminotransferase (EC 2.6.1.1... 29 8.8
22WASIP_MOUSE (Q8K1I7) Wiskott-Aldrich syndrome protein-interactin... 29 8.8
23CP46A_HUMAN (Q9Y6A2) Cytochrome p450 46A1 (EC 1.14.13.98) (Chole... 29 8.8

>TRK1_SACBA (P28569) High-affinity potassium transport protein|
          Length = 1241

 Score = 37.0 bits (84), Expect = 0.042
 Identities = 17/50 (34%), Positives = 30/50 (60%)
 Frame = -1

Query: 395 EDLQPQI*FPHQKRRMGGHI*AHLHPTRPTKDRLPRDFARSAI*ENNTSE 246
           +DL P+  FPHQK+   G+I  HL   R  + ++ R  +  +I +N++S+
Sbjct: 460 DDLNPRSIFPHQKKSSKGYIMKHLPKARRIRQQIKRRLSTGSIDKNSSSD 509



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>PHLB_MYCTU (P95246) Phospholipase C 2 precursor (EC 3.1.4.3)|
          Length = 521

 Score = 33.9 bits (76), Expect = 0.36
 Identities = 35/142 (24%), Positives = 54/142 (38%), Gaps = 29/142 (20%)
 Frame = -2

Query: 580 VPSPDGLRGPVPFFFKFDRLGVRVPTMMVSPWIKKGMVVGRPPNGPTATSEYEHSSIPAT 401
           VP   G+RGP+        LG RVP +++SP+ +  ++V            ++H+S    
Sbjct: 384 VPGSGGIRGPIG-------LGFRVPCLVISPYSRGPLMV---------HDTFDHTSTLKL 427

Query: 400 IKKIFNLRSDFLTK-RDEWAGTFEHIFT----------QLDQPRTDCPETLPE------- 275
           I+  F +    LT  RD   G     F            LD PR +    LP+       
Sbjct: 428 IRARFGVPVPNLTAWRDATVGDMTSTFNFAAPPNPSKPNLDHPRLNALPKLPQCVPNAVL 487

Query: 274 -----------VPFERTTPAKE 242
                      VPF ++ P +E
Sbjct: 488 GTVTKTAIPYRVPFPQSMPTQE 509



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>PHLC_MYCTU (P95245) Phospholipase C 3 precursor (EC 3.1.4.3)|
          Length = 517

 Score = 33.5 bits (75), Expect = 0.47
 Identities = 35/137 (25%), Positives = 52/137 (37%), Gaps = 22/137 (16%)
 Frame = -2

Query: 580 VPSPDGLRGPVPFFFKFDRLGVRVPTMMVSPWIKKGMVV-------------GRPPNGPT 440
           V    G+RGP+        LG RVP  ++SP+ + G++V             G+    P 
Sbjct: 382 VDGSGGIRGPIG-------LGFRVPCFVISPYSRGGLMVHDRFDHTSQLQLIGKRFGVPV 434

Query: 439 ATSEYEHSSIPATIKKIFNLRSDFLTKRDEWAGTFEHIFTQLDQPRTDCP--------ET 284
                  +S+   +   FN    F    D      +H   QL +     P        E 
Sbjct: 435 PNLTPWRASVTGDMTSAFN----FAAPPDPSPPNLDHPVRQLPKVAKCVPNVVLGFLNEG 490

Query: 283 LP-EVPFERTTPAKEHG 236
           LP  VP+ +TTP +E G
Sbjct: 491 LPYRVPYPQTTPVQESG 507



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>NUPL_HUMAN (P52594) Nucleoporin-like protein RIP (HIV-1 Rev-binding protein)|
           (Rev-interacting protein) (Rev/Rex activation
           domain-binding protein)
          Length = 562

 Score = 32.0 bits (71), Expect = 1.4
 Identities = 28/121 (23%), Positives = 47/121 (38%), Gaps = 9/121 (7%)
 Frame = +3

Query: 252 GVVLSNGTSGKVSGQSVLGWSSWVKMCSNVPAHSSLLVRKSDLRLKIF---LMVAGMEEC 422
           G V+S  +    S       +    + S+    S+     S+    +F    +VA  +  
Sbjct: 358 GSVVSVPSQSSASSDKYAALAELDSVFSSAATSSNAYTSTSNASSNVFGTVPVVASAQTQ 417

Query: 423 SYSDVAVGPFGGRPTTIPFLIHGDTIIVGTLTPSRSNLK-----KNGTGPL-RPSGLGTP 584
             S     PFG  P+T PF+      +  +  P ++N +       GT  +  P+G GTP
Sbjct: 418 PASSSVPAPFGATPSTNPFVAAAGPSVASSTNPFQTNARGATAATFGTASMSMPTGFGTP 477

Query: 585 A 587
           A
Sbjct: 478 A 478



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>PHLN_BURPS (Q9RGS8) Non-hemolytic phospholipase C precursor (EC 3.1.4.3)|
           (PLC-N) (Phosphatidylcholine cholinephosphohydrolase)
           (Phosphatidylcholine-hydrolyzing phospholipase C)
           (PC-PLC)
          Length = 700

 Score = 32.0 bits (71), Expect = 1.4
 Identities = 18/49 (36%), Positives = 26/49 (53%)
 Frame = -2

Query: 523 LGVRVPTMMVSPWIKKGMVVGRPPNGPTATSEYEHSSIPATIKKIFNLR 377
           LG RVP ++VSPW K G V          +  ++H+S+   I+  FN R
Sbjct: 395 LGPRVPMLVVSPWTKGGWV---------CSQTFDHTSLLQFIEARFNDR 434



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>UBE3A_MOUSE (O08759) Ubiquitin-protein ligase E3A (EC 6.3.2.-) (Oncogenic|
           protein-associated protein E6-AP)
          Length = 885

 Score = 31.6 bits (70), Expect = 1.8
 Identities = 14/37 (37%), Positives = 20/37 (54%)
 Frame = +2

Query: 302 PWLVELGEDVLKCARPFVSFGEEIRSEVEDLLDGRRD 412
           P   ELG   L C +P +SF E I   + D+L+  +D
Sbjct: 438 PLETELGVKTLDCRKPLISFEESINEPLNDVLEMDKD 474



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>PDP_BACSU (P39142) Pyrimidine-nucleoside phosphorylase (EC 2.4.2.2) (PYNP)|
          Length = 433

 Score = 31.2 bits (69), Expect = 2.3
 Identities = 14/44 (31%), Positives = 24/44 (54%)
 Frame = -2

Query: 313 DQPRTDCPETLPEVPFERTTPAKEHGILSDFQRELVELASFLNG 182
           D    D P  LP+  ++   PAKE G++S+   + + +A+ L G
Sbjct: 319 DSSIVDDPSKLPQAAYQIDVPAKEAGVVSEIVADEIGVAAMLLG 362



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>NUPL_BOVIN (Q2TA45) Nucleoporin-like protein RIP (HIV-1 Rev-binding protein|
           homolog)
          Length = 562

 Score = 30.8 bits (68), Expect = 3.0
 Identities = 28/121 (23%), Positives = 46/121 (38%), Gaps = 9/121 (7%)
 Frame = +3

Query: 252 GVVLSNGTSGKVSGQSVLGWSSWVKMCSNVPAHSSLLVRKSDLRLKIFLMV---AGMEEC 422
           G V+S  +    S       +    + S+    S+     S+    +F  V   A  +  
Sbjct: 358 GSVVSVPSQSSASSDKYAALAELDSVFSSAATSSNAYTSTSNASSNVFGTVPVGASAQTQ 417

Query: 423 SYSDVAVGPFGGRPTTIPFLIHGDTIIVGTLTPSRSNLK-----KNGTGPL-RPSGLGTP 584
             S     PFG  P+T PF+      +  +  P ++N +       GT  +  P+G GTP
Sbjct: 418 PASSSVPAPFGATPSTNPFVAAAGPSVASSTNPFQTNARGATAATFGTASMSMPAGFGTP 477

Query: 585 A 587
           A
Sbjct: 478 A 478



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>RPO3B_TOBAC (Q8L6J1) DNA-directed RNA polymerase 3B, chloroplast precursor (EC|
           2.7.7.6) (T7 bacteriophage-type single subunit RNA
           polymerase 3B) (NictaRpoT3-tom)
          Length = 977

 Score = 30.8 bits (68), Expect = 3.0
 Identities = 15/41 (36%), Positives = 25/41 (60%)
 Frame = +3

Query: 204 STSSRWKSERIPCSFAGVVLSNGTSGKVSGQSVLGWSSWVK 326
           S +S+W+++++P  F   V+ N   GK+S  S L  SS+ K
Sbjct: 10  SPTSQWRTQKLPKRFNFYVIHNQEFGKLSQSSSLSTSSFPK 50



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>PHLA_MYCTU (Q04001) Phospholipase C 1 precursor (EC 3.1.4.3) (MTP40 antigen)|
          Length = 520

 Score = 30.8 bits (68), Expect = 3.0
 Identities = 30/113 (26%), Positives = 47/113 (41%), Gaps = 9/113 (7%)
 Frame = -2

Query: 580 VPSPDGLRGPVPFFFKFDRLGVRVPTMMVSPWIKKGMVVGRPPNGPTATSE-YEHSSIPA 404
           VP   G+RGP+        LG RVP +++SP+ +          GP   S+ ++H+S   
Sbjct: 380 VPGSGGIRGPLG-------LGFRVPCIVISPYSR----------GPLMVSDTFDHTSQLK 422

Query: 403 TIKKIFNLRSDFLTK-RDEWAGTFEHIFTQLDQPRTDCPE-------TLPEVP 269
            I+  F +    +T  RD   G     F     P +  P         LP++P
Sbjct: 423 LIRARFGVPVPNMTAWRDGVVGDMTSAFNFATPPNSTRPNLSHPLLGALPKLP 475



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>MMP9_MOUSE (P41245) Matrix metalloproteinase-9 precursor (EC 3.4.24.35)|
           (MMP-9) (92 kDa type IV collagenase) (92 kDa gelatinase)
           (Gelatinase B) (GELB)
          Length = 730

 Score = 30.4 bits (67), Expect = 4.0
 Identities = 21/65 (32%), Positives = 28/65 (43%), Gaps = 8/65 (12%)
 Frame = +2

Query: 245 FRWCCSLKWHFWQSLWAICPWLVE--------LGEDVLKCARPFVSFGEEIRSEVEDLLD 400
           +RWC +   +    L+  CP  V+         GE    C  PFV  G++  S      D
Sbjct: 311 YRWCATTANYDQDKLYGFCPTRVDATVVGGNSAGE---LCVFPFVFLGKQYSSCTS---D 364

Query: 401 GRRDG 415
           GRRDG
Sbjct: 365 GRRDG 369



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>KITH_HAEDU (Q7VLH2) Thymidine kinase (EC 2.7.1.21)|
          Length = 198

 Score = 30.4 bits (67), Expect = 4.0
 Identities = 25/90 (27%), Positives = 41/90 (45%), Gaps = 4/90 (4%)
 Frame = -2

Query: 283 LPEVPFERTTPAKEHGILSDFQRELVELASFLNGDYMLTSFAQETQKKMTV----KQADT 116
           L +V  E   P   +G+ +DFQ+EL E      G   L ++A E Q+  T+    K+A  
Sbjct: 100 LTDVVDELKIPVLCYGLRTDFQKELFE------GSQYLLAWADELQELKTICECGKKAHF 153

Query: 115 YVRRAIKGFLHASRQARRLGANESAIVTMR 26
            +R    G    + +  ++G N+  I   R
Sbjct: 154 VIRLNENGETVTTGEQIQIGGNDKYISVCR 183



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>RPOT3_NICSY (P69242) DNA-directed RNA polymerase 3, chloroplast precursor (EC|
           2.7.7.6) (T7 bacteriophage-type single subunit RNA
           polymerase 3) (NsRpoT-C)
          Length = 977

 Score = 30.0 bits (66), Expect = 5.2
 Identities = 15/41 (36%), Positives = 25/41 (60%)
 Frame = +3

Query: 204 STSSRWKSERIPCSFAGVVLSNGTSGKVSGQSVLGWSSWVK 326
           S +S+W+++++P  F   V+ N   GK+S  S L  SS+ K
Sbjct: 10  SPTSQWRTQKLPKRFNFYVIHNQEFGKLSQSSSLPTSSFPK 50



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>RPO3A_TOBAC (P69243) DNA-directed RNA polymerase 3A, chloroplast precursor (EC|
           2.7.7.6) (T7 bacteriophage-type single subunit RNA
           polymerase 3A) (NictaRpoT3-syl)
          Length = 977

 Score = 30.0 bits (66), Expect = 5.2
 Identities = 15/41 (36%), Positives = 25/41 (60%)
 Frame = +3

Query: 204 STSSRWKSERIPCSFAGVVLSNGTSGKVSGQSVLGWSSWVK 326
           S +S+W+++++P  F   V+ N   GK+S  S L  SS+ K
Sbjct: 10  SPTSQWRTQKLPKRFNFYVIHNQEFGKLSQSSSLPTSSFPK 50



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>GSH1_PHOLL (Q7N7A4) Glutamate--cysteine ligase (EC 6.3.2.2)|
           (Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS)
          Length = 519

 Score = 29.6 bits (65), Expect = 6.7
 Identities = 15/51 (29%), Positives = 25/51 (49%)
 Frame = +2

Query: 287 LWAICPWLVELGEDVLKCARPFVSFGEEIRSEVEDLLDGRRDGGVLVLGCG 439
           +W +     E+G D L C R         ++    +L+GR+ G ++ LGCG
Sbjct: 355 IWCVLAEAPEMGSDELDCCR---------KNWNRVILEGRKPGQMIGLGCG 396



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>UCRIC_ANASP (P70758) Cytochrome b6-f complex iron-sulfur subunit 3 (EC|
           1.10.99.1) (Rieske iron-sulfur protein 3)
           (Plastohydroquinone:plastocyanin oxidoreductase
           iron-sulfur protein 3) (ISP 3) (RISP 3)
          Length = 178

 Score = 29.6 bits (65), Expect = 6.7
 Identities = 14/43 (32%), Positives = 24/43 (55%), Gaps = 2/43 (4%)
 Frame = -2

Query: 574 SPDG--LRGPVPFFFKFDRLGVRVPTMMVSPWIKKGMVVGRPP 452
           +PDG  +RGP P   K  ++ V   ++++SPW +     G+ P
Sbjct: 133 APDGSVVRGPAPLPLKIVQVAVIDNSILISPWTETDPRTGKKP 175



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>WASIP_RAT (Q6IN36) Wiskott-Aldrich syndrome protein-interacting protein|
           (WASP-interacting protein)
          Length = 487

 Score = 29.3 bits (64), Expect = 8.8
 Identities = 28/116 (24%), Positives = 43/116 (37%), Gaps = 4/116 (3%)
 Frame = -2

Query: 580 VPSPDGLRGPVPFFFKFDRLGVRVPTMMVSPWIKKGMVVGRPPNGPTATSEYEH--SSIP 407
           V  P G  GP+P     +R G     +  +P +     +  P +GP      +   +  P
Sbjct: 353 VRDPPGRSGPLPPPPPINRNGSTARALPATPQLPSRSGMDSPRSGPRPPLPPDRPGAGAP 412

Query: 406 ATIKKIFNLRSDFLTK--RDEWAGTFEHIFTQLDQPRTDCPETLPEVPFERTTPAK 245
                  ++R+ F      DEW   F         P +D P   P VP  +T P+K
Sbjct: 413 PPPPPSTSVRNGFQDSSCEDEWESRFYF------HPISDLPPPEPYVPTTKTYPSK 462



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>SCNNA_BOVIN (P55270) Amiloride-sensitive sodium channel alpha-subunit|
           (Epithelial Na+ channel alpha subunit) (Alpha ENaC)
           (Nonvoltage-gated sodium channel 1 alpha subunit)
           (SCNEA) (Alpha NaCH)
          Length = 650

 Score = 29.3 bits (64), Expect = 8.8
 Identities = 11/32 (34%), Positives = 19/32 (59%)
 Frame = +3

Query: 39  IADSFAPNRLACLLACRKPLMARLTYVSACFT 134
           + D+F+ +RL C   CRKP    +  +SA ++
Sbjct: 441 LQDAFSSDRLGCFTKCRKPCSVTIYKLSASYS 472



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>MYH6_HUMAN (P13533) Myosin heavy chain, cardiac muscle alpha isoform|
            (MyHC-alpha)
          Length = 1939

 Score = 29.3 bits (64), Expect = 8.8
 Identities = 26/86 (30%), Positives = 37/86 (43%)
 Frame = -2

Query: 397  KKIFNLRSDFLTKRDEWAGTFEHIFTQLDQPRTDCPETLPEVPFERTTPAKEHGILSDFQ 218
            KK F++        DE A   + +  +L + +    E   E+  ERT  AK   + SD  
Sbjct: 1084 KKEFDINQQNSKIEDEQALALQ-LQKKLKENQARIEELEEELEAERTARAKVEKLRSDLS 1142

Query: 217  RELVELASFLNGDYMLTSFAQETQKK 140
            REL E++  L      TS   E  KK
Sbjct: 1143 RELEEISERLEEAGGATSVQIEMNKK 1168



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>GLMM_DESPS (Q6AMQ5) Phosphoglucosamine mutase (EC 5.4.2.10)|
          Length = 450

 Score = 29.3 bits (64), Expect = 8.8
 Identities = 20/67 (29%), Positives = 35/67 (52%), Gaps = 1/67 (1%)
 Frame = +3

Query: 243 SFAGVVLSNGTSGKVSGQSVLGWSSWVKMCS-NVPAHSSLLVRKSDLRLKIFLMVAGMEE 419
           SF  V+ +  T+ K+S  S++G++  VK     +     +LVR S     I +MV G++ 
Sbjct: 371 SFPQVLKNVRTAKKISVDSIVGFADAVKKYEMQLGDTGRILVRPSGTEPVIRVMVEGLDS 430

Query: 420 CSYSDVA 440
              +D+A
Sbjct: 431 AEINDIA 437



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>ARGD_BACAN (Q81M98) Acetylornithine aminotransferase (EC 2.6.1.11) (ACOAT)|
          Length = 386

 Score = 29.3 bits (64), Expect = 8.8
 Identities = 16/33 (48%), Positives = 19/33 (57%)
 Frame = +3

Query: 486 HGDTIIVGTLTPSRSNLKKNGTGPLRPSGLGTP 584
           HG T   GT++ +  N  K G GPL PS L TP
Sbjct: 124 HGRTF--GTMSATGQNKVKEGFGPLLPSFLHTP 154



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>WASIP_MOUSE (Q8K1I7) Wiskott-Aldrich syndrome protein-interacting protein|
           (WASP-interacting protein)
          Length = 493

 Score = 29.3 bits (64), Expect = 8.8
 Identities = 28/116 (24%), Positives = 43/116 (37%), Gaps = 4/116 (3%)
 Frame = -2

Query: 580 VPSPDGLRGPVPFFFKFDRLGVRVPTMMVSPWIKKGMVVGRPPNGPTATSEYEH--SSIP 407
           V  P G  GP+P     +R G     +  +P +     +  P +GP      +   +  P
Sbjct: 359 VRDPPGRSGPLPPPPPINRNGSTARALPATPQLPSRSGMDSPRSGPRPPLPPDRPGAGAP 418

Query: 406 ATIKKIFNLRSDFLTK--RDEWAGTFEHIFTQLDQPRTDCPETLPEVPFERTTPAK 245
                  ++R+ F      DEW   F         P +D P   P VP  +T P+K
Sbjct: 419 PPPPPSTSVRNGFQDSSCEDEWESRFYF------HPISDLPPPEPYVPTTKTYPSK 468



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>CP46A_HUMAN (Q9Y6A2) Cytochrome p450 46A1 (EC 1.14.13.98) (Cholesterol|
           24-hydroxylase) (CH24H)
          Length = 500

 Score = 29.3 bits (64), Expect = 8.8
 Identities = 15/36 (41%), Positives = 19/36 (52%)
 Frame = -2

Query: 583 GVPSPDGLRGPVPFFFKFDRLGVRVPTMMVSPWIKK 476
           G P P  L G +P F+K D +G RV   +   W KK
Sbjct: 35  GPPRPSFLLGHLPCFWKKDEVGGRVLQDVFLDWAKK 70


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,483,245
Number of Sequences: 219361
Number of extensions: 1503506
Number of successful extensions: 5031
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 4831
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5022
length of database: 80,573,946
effective HSP length: 106
effective length of database: 57,321,680
effective search space used: 5101629520
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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