ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日：2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd22o08
Clone Library Name barley_pub
No. Definition Score
(bits)		 E
Value
1ref|XM_463165.1| Oryza sativa (japonica cultivar-group), mRNA 192 6e-46
2dbj|AK065587.1| Oryza sativa (japonica cultivar-group) cDNA clon... 184 2e-43
3gb|DP000009.1| Oryza sativa (japonica cultivar-group) chromosome 3 172 6e-40
4gb|AC136972.3| Oryza sativa chromosome 3 BAC OSJNBb0007E22 genom... 172 6e-40
5dbj|AP008209.1| Oryza sativa (japonica cultivar-group) genomic D... 172 6e-40
6gb|AE009072.1| Agrobacterium tumefaciens str. C58 circular chrom... 56 1e-04
7gb|AE007869.1| Agrobacterium tumefaciens str. C58, complete genome 56 1e-04
8ref|XM_658407.1| Aspergillus nidulans FGSC A4 hypothetical prote... 44 0.37
9gb|AC144636.4| Artibeus jamaicensis clone 1A13, complete sequence 44 0.37
10gb|DP000001.1| Artibeus jamaicensis target 1 genomic scaffold 44 0.37
11gb|AC016708.11| Homo sapiens BAC clone RP11-223M6 from 2, comple... 42 1.5
12ref|NM_125079.2| Arabidopsis thaliana CKX3 (CYTOKININ OXIDASE 3)... 40 5.8
13ref|XM_638766.1| Dictyostelium discoideum hypothetical protein (... 40 5.8
14gb|AC068607.5| Mus musculus strain C57BL6/J chromosome 6 clone R... 40 5.8
15gb|U91324.1|HUU91324 Homo sapiens Chromosome 16 BAC clone CIT987... 40 5.8
16gb|AY935269.1| Antirrhinum majus plena (PLE) gene, complete sequ... 40 5.8
17emb|AL691420.13| Human DNA sequence from clone RP11-352C6 on chr... 40 5.8
18gb|CP000284.1| Methylobacillus flagellatus KT, complete genome 40 5.8
19emb|AJ300474.1|AHI300474 Antirrhinum hispanicum ORF1, ORF2, ORF3... 40 5.8
20gb|AC090150.9| Homo sapiens chromosome 8, clone RP11-521M14, com... 40 5.8
21gb|AC124649.6| Homo sapiens chromosome 8, clone RP11-23I8, compl... 40 5.8
22gb|AE010300.1| Leptospira interrogans serovar lai str. 56601 chr... 40 5.8
23emb|BX830070.1|CNS0A1GX Arabidopsis thaliana Full-length cDNA Co... 40 5.8
24gb|AC107387.6| Homo sapiens BAC clone RP11-321I11 from 2, comple... 40 5.8
25gb|AC116305.2| Dictyostelium discoideum chromosome 2 map 1005175... 40 5.8
26gb|AF303979.1|AF303979 Arabidopsis thaliana cytokinin oxidase (C... 40 5.8
27dbj|AB024035.1| Arabidopsis thaliana genomic DNA, chromosome 5, ... 40 5.8
28dbj|AP006255.1| Homo sapiens genomic DNA, chromosome 9, clone:RP... 40 5.8
29gb|AY552545.2| Uncultured marine gamma proteobacterium EBAC20E09... 40 5.8
30gb|AE008692.1| Zymomonas mobilis subsp. mobilis ZM4, complete ge... 40 5.8
31emb|AL671881.8| Mouse DNA sequence from clone RP23-206E1 on chro... 40 5.8
32dbj|AB020871.1| Homo sapiens genomic DNA of 9q32 anti-oncogene o... 40 5.8 
>ref|XM_463165.1| Oryza sativa (japonica cultivar-group),  mRNA
          Length = 1618

 Score =  192 bits (97), Expect = 6e-46
 Identities = 183/212 (86%)
 Strand = Plus / Minus

                                                                        
Query: 219  atgtcatcgaggacttgcggcggcagaggccgcggagcgctggtaaatgcatcgatgttc 278
            |||||||| || |||| ||| | || |||||| ||||| |||||||||||||| ||||||
Sbjct: 1332 atgtcatcaagaacttccggtgccaaaggccgtggagcactggtaaatgcatcaatgttc 1273

                                                                        
Query: 279  tccttcaactgatccatggtggtcgctccgatgatggtgctggctgtaaaagggcggtca 338
            |||||||||||||||||||| || ||||||||||| ||||| ||||| || || ||||||
Sbjct: 1272 tccttcaactgatccatggtcgttgctccgatgattgtgctagctgtgaatggacggtca 1213

                                                                        
Query: 339  cgcacgaagccaanggcaagctggacaggtgttagcccatgcttcttggcaagcttgaca 398
            ||||| ||||| |  ||||||||||| || ||||| || |||||||||||||||||||||
Sbjct: 1212 cgcacaaagcccagtgcaagctggactggagttaggccgtgcttcttggcaagcttgaca 1153

                                            
Query: 399  tactcgtctgttgcttctttagccaaagatgc 430
            || || |  ||||||||||||||||| |||||
Sbjct: 1152 tattcattcgttgcttctttagccaacgatgc 1121
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>dbj|AK065587.1| Oryza sativa (japonica cultivar-group) cDNA clone:J013030E14, full
            insert sequence
          Length = 1606

 Score =  184 bits (93), Expect = 2e-43
 Identities = 182/212 (85%)
 Strand = Plus / Minus

                                                                        
Query: 219  atgtcatcgaggacttgcggcggcagaggccgcggagcgctggtaaatgcatcgatgttc 278
            |||||||| || |||| ||| | || |||||| ||||| |||||||||||||| ||||||
Sbjct: 1370 atgtcatcaagaacttccggtgccaaaggccgtggagcactggtaaatgcatcaatgttc 1311

                                                                        
Query: 279  tccttcaactgatccatggtggtcgctccgatgatggtgctggctgtaaaagggcggtca 338
            |||||||||||||||||||| || ||||||||||| ||||| ||||| ||  | ||||||
Sbjct: 1310 tccttcaactgatccatggtcgttgctccgatgattgtgctagctgtgaatcgacggtca 1251

                                                                        
Query: 339  cgcacgaagccaanggcaagctggacaggtgttagcccatgcttcttggcaagcttgaca 398
            ||||| ||||| |  ||||||||||| || ||||| || |||||||||||||||||||||
Sbjct: 1250 cgcacaaagcccagtgcaagctggactggagttaggccgtgcttcttggcaagcttgaca 1191

                                            
Query: 399  tactcgtctgttgcttctttagccaaagatgc 430
            || || |  ||||||||||||||||| |||||
Sbjct: 1190 tattcattcgttgcttctttagccaacgatgc 1159
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>gb|DP000009.1| Oryza sativa (japonica cultivar-group) chromosome 3
          Length = 36102668

 Score =  172 bits (87), Expect = 6e-40
 Identities = 158/182 (86%)
 Strand = Plus / Minus

                                                                            
Query: 219      atgtcatcgaggacttgcggcggcagaggccgcggagcgctggtaaatgcatcgatgttc 278
                |||||||| || |||| ||| | || |||||| ||||| |||||||||||||| ||||||
Sbjct: 22897858 atgtcatcaagaacttccggtgccaaaggccgtggagcactggtaaatgcatcaatgttc 22897799

                                                                            
Query: 279      tccttcaactgatccatggtggtcgctccgatgatggtgctggctgtaaaagggcggtca 338
                |||||||||||||||||||| || ||||||||||| ||||| ||||| || || ||||||
Sbjct: 22897798 tccttcaactgatccatggtcgttgctccgatgattgtgctagctgtgaatggacggtca 22897739

                                                                            
Query: 339      cgcacgaagccaanggcaagctggacaggtgttagcccatgcttcttggcaagcttgaca 398
                ||||| ||||| |  ||||||||||| || ||||| || |||||||||||||||||||||
Sbjct: 22897738 cgcacaaagcccagtgcaagctggactggagttaggccgtgcttcttggcaagcttgaca 22897679

                  
Query: 399      ta 400
                ||
Sbjct: 22897678 ta 22897677
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>gb|AC136972.3| Oryza sativa chromosome 3 BAC OSJNBb0007E22 genomic sequence, complete
              sequence
          Length = 184783

 Score =  172 bits (87), Expect = 6e-40
 Identities = 158/182 (86%)
 Strand = Plus / Minus

                                                                          
Query: 219    atgtcatcgaggacttgcggcggcagaggccgcggagcgctggtaaatgcatcgatgttc 278
              |||||||| || |||| ||| | || |||||| ||||| |||||||||||||| ||||||
Sbjct: 100388 atgtcatcaagaacttccggtgccaaaggccgtggagcactggtaaatgcatcaatgttc 100329

                                                                          
Query: 279    tccttcaactgatccatggtggtcgctccgatgatggtgctggctgtaaaagggcggtca 338
              |||||||||||||||||||| || ||||||||||| ||||| ||||| || || ||||||
Sbjct: 100328 tccttcaactgatccatggtcgttgctccgatgattgtgctagctgtgaatggacggtca 100269

                                                                          
Query: 339    cgcacgaagccaanggcaagctggacaggtgttagcccatgcttcttggcaagcttgaca 398
              ||||| ||||| |  ||||||||||| || ||||| || |||||||||||||||||||||
Sbjct: 100268 cgcacaaagcccagtgcaagctggactggagttaggccgtgcttcttggcaagcttgaca 100209

                
Query: 399    ta 400
              ||
Sbjct: 100208 ta 100207
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>dbj|AP008209.1| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 3, complete
                sequence
          Length = 36192742

 Score =  172 bits (87), Expect = 6e-40
 Identities = 158/182 (86%)
 Strand = Plus / Minus

                                                                            
Query: 219      atgtcatcgaggacttgcggcggcagaggccgcggagcgctggtaaatgcatcgatgttc 278
                |||||||| || |||| ||| | || |||||| ||||| |||||||||||||| ||||||
Sbjct: 22890886 atgtcatcaagaacttccggtgccaaaggccgtggagcactggtaaatgcatcaatgttc 22890827

                                                                            
Query: 279      tccttcaactgatccatggtggtcgctccgatgatggtgctggctgtaaaagggcggtca 338
                |||||||||||||||||||| || ||||||||||| ||||| ||||| || || ||||||
Sbjct: 22890826 tccttcaactgatccatggtcgttgctccgatgattgtgctagctgtgaatggacggtca 22890767

                                                                            
Query: 339      cgcacgaagccaanggcaagctggacaggtgttagcccatgcttcttggcaagcttgaca 398
                ||||| ||||| |  ||||||||||| || ||||| || |||||||||||||||||||||
Sbjct: 22890766 cgcacaaagcccagtgcaagctggactggagttaggccgtgcttcttggcaagcttgaca 22890707

                  
Query: 399      ta 400
                ||
Sbjct: 22890706 ta 22890705
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>gb|AE009072.1| Agrobacterium tumefaciens str. C58 circular chromosome, section 98 of
             256 of the complete sequence
          Length = 11368

 Score = 56.0 bits (28), Expect = 1e-04
 Identities = 34/36 (94%)
 Strand = Plus / Plus

                                                 
Query: 280   ccttcaactgatccatggtggtcgctccgatgatgg 315
             |||||||||| |||||||||||||| ||||||||||
Sbjct: 10300 ccttcaactgttccatggtggtcgcgccgatgatgg 10335
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>gb|AE007869.1| Agrobacterium tumefaciens str. C58, complete genome
          Length = 2841581

 Score = 56.0 bits (28), Expect = 1e-04
 Identities = 34/36 (94%)
 Strand = Plus / Plus

                                                   
Query: 280     ccttcaactgatccatggtggtcgctccgatgatgg 315
               |||||||||| |||||||||||||| ||||||||||
Sbjct: 1082656 ccttcaactgttccatggtggtcgcgccgatgatgg 1082691
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>ref|XM_658407.1| Aspergillus nidulans FGSC A4 hypothetical protein (AN5895.2), mRNA
          Length = 1407

 Score = 44.1 bits (22), Expect = 0.37
 Identities = 25/26 (96%)
 Strand = Plus / Minus

                                     
Query: 197 gtatttcttgaaaagaatctcaatgt 222
           |||||||||||||||| |||||||||
Sbjct: 174 gtatttcttgaaaagagtctcaatgt 149
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>gb|AC144636.4| Artibeus jamaicensis clone 1A13, complete sequence
          Length = 114691

 Score = 44.1 bits (22), Expect = 0.37
 Identities = 22/22 (100%)
 Strand = Plus / Minus

                                   
Query: 9     acatatatttcaaaaaataaac 30
             ||||||||||||||||||||||
Sbjct: 93806 acatatatttcaaaaaataaac 93785
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>gb|DP000001.1| Artibeus jamaicensis target 1 genomic scaffold
          Length = 584809

 Score = 44.1 bits (22), Expect = 0.37
 Identities = 22/22 (100%)
 Strand = Plus / Minus

                                    
Query: 9      acatatatttcaaaaaataaac 30
              ||||||||||||||||||||||
Sbjct: 418365 acatatatttcaaaaaataaac 418344
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>gb|AC016708.11| Homo sapiens BAC clone RP11-223M6 from 2, complete sequence
          Length = 194304

 Score = 42.1 bits (21), Expect = 1.5
 Identities = 21/21 (100%)
 Strand = Plus / Minus

                                 
Query: 196  tgtatttcttgaaaagaatct 216
            |||||||||||||||||||||
Sbjct: 1820 tgtatttcttgaaaagaatct 1800
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>ref|NM_125079.2| Arabidopsis thaliana CKX3 (CYTOKININ OXIDASE 3); cytokinin oxidase
            AT5G56970 (CKX3) mRNA, complete cds
          Length = 1595

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                
Query: 11   atatatttcaaaaaataaac 30
            ||||||||||||||||||||
Sbjct: 1567 atatatttcaaaaaataaac 1586
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>ref|XM_638766.1| Dictyostelium discoideum hypothetical protein (DDB0167375), partial
           mRNA
          Length = 1230

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 29/32 (90%)
 Strand = Plus / Minus

                                           
Query: 191 gtctttgtatttcttgaaaagaatctcaatgt 222
           ||||||| |||| ||||||||||| |||||||
Sbjct: 335 gtctttggatttgttgaaaagaatttcaatgt 304
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>gb|AC068607.5| Mus musculus strain C57BL6/J chromosome 6 clone RP23-392C14, complete
              sequence
          Length = 218081

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                  
Query: 8      cacatatatttcaaaaaata 27
              ||||||||||||||||||||
Sbjct: 127206 cacatatatttcaaaaaata 127187
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>gb|U91324.1|HUU91324 Homo sapiens Chromosome 16 BAC clone CIT987-SKA-100B4  ~complete genomic
              sequence, complete sequence
          Length = 174826

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                  
Query: 134    tgaggattgatgccctttgt 153
              ||||||||||||||||||||
Sbjct: 172802 tgaggattgatgccctttgt 172783
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>gb|AY935269.1| Antirrhinum majus plena (PLE) gene, complete sequence
          Length = 85060

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                 
Query: 6     tgcacatatatttcaaaaaa 25
             ||||||||||||||||||||
Sbjct: 38898 tgcacatatatttcaaaaaa 38917
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>emb|AL691420.13| Human DNA sequence from clone RP11-352C6 on chromosome 9, complete
             sequence
          Length = 175726

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 406   ctgttgcttctttagccaaa 425
             ||||||||||||||||||||
Sbjct: 20964 ctgttgcttctttagccaaa 20945
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>gb|CP000284.1| Methylobacillus flagellatus KT, complete genome
          Length = 2971517

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 23/24 (95%)
 Strand = Plus / Plus

                                      
Query: 298    tggtcgctccgatgatggtgctgg 321
              ||||||| ||||||||||||||||
Sbjct: 409609 tggtcgcgccgatgatggtgctgg 409632
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>emb|AJ300474.1|AHI300474 Antirrhinum hispanicum ORF1, ORF2, ORF3, ORF4, ORF5, ORF6, ORF7, ORF8,
             s2-RNase gene, ORF9 and slf-S2 gene
          Length = 63687

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                 
Query: 11    atatatttcaaaaaataaac 30
             ||||||||||||||||||||
Sbjct: 48634 atatatttcaaaaaataaac 48653
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>gb|AC090150.9| Homo sapiens chromosome 8, clone RP11-521M14, complete sequence
          Length = 210629

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                  
Query: 353    ggcaagctggacaggtgtta 372
              ||||||||||||||||||||
Sbjct: 170753 ggcaagctggacaggtgtta 170734
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>gb|AC124649.6| Homo sapiens chromosome 8, clone RP11-23I8, complete sequence
          Length = 191004

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 353   ggcaagctggacaggtgtta 372
             ||||||||||||||||||||
Sbjct: 57405 ggcaagctggacaggtgtta 57386
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>gb|AE010300.1| Leptospira interrogans serovar lai str. 56601 chromosome I, complete
               sequence
          Length = 4332241

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 23/24 (95%)
 Strand = Plus / Plus

                                       
Query: 41      ttgggaaatatcagttgaaagaaa 64
               ||||||||||| ||||||||||||
Sbjct: 1764894 ttgggaaatattagttgaaagaaa 1764917
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>emb|BX830070.1|CNS0A1GX Arabidopsis thaliana Full-length cDNA Complete sequence from clone
            GSLTFB57ZE08 of Flowers and buds of strain col-0 of
            Arabidopsis thaliana (thale cress)
          Length = 1594

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                
Query: 11   atatatttcaaaaaataaac 30
            ||||||||||||||||||||
Sbjct: 1567 atatatttcaaaaaataaac 1586
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>gb|AC107387.6| Homo sapiens BAC clone RP11-321I11 from 2, complete sequence
          Length = 105842

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                
Query: 34   tacacatttgggaaatatca 53
            ||||||||||||||||||||
Sbjct: 7650 tacacatttgggaaatatca 7669
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>gb|AC116305.2| Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4,
             complete sequence
          Length = 413138

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 29/32 (90%)
 Strand = Plus / Plus

                                             
Query: 191   gtctttgtatttcttgaaaagaatctcaatgt 222
             ||||||| |||| ||||||||||| |||||||
Sbjct: 22534 gtctttggatttgttgaaaagaatttcaatgt 22565
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>gb|AF303979.1|AF303979 Arabidopsis thaliana cytokinin oxidase (CKX3) mRNA, complete cds
          Length = 1572

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                
Query: 11   atatatttcaaaaaataaac 30
            ||||||||||||||||||||
Sbjct: 1544 atatatttcaaaaaataaac 1563
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>dbj|AB024035.1| Arabidopsis thaliana genomic DNA, chromosome 5, P1 clone:MHM17
          Length = 78423

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 11    atatatttcaaaaaataaac 30
             ||||||||||||||||||||
Sbjct: 29443 atatatttcaaaaaataaac 29424
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>dbj|AP006255.1| Homo sapiens genomic DNA, chromosome 9, clone:RP11-361G14, complete
             sequence
          Length = 189176

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 406   ctgttgcttctttagccaaa 425
             ||||||||||||||||||||
Sbjct: 49560 ctgttgcttctttagccaaa 49541
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>gb|AY552545.2| Uncultured marine gamma proteobacterium EBAC20E09 clone EBAC20E09,
             complete sequence
          Length = 144407

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 11    atatatttcaaaaaataaac 30
             ||||||||||||||||||||
Sbjct: 64348 atatatttcaaaaaataaac 64329
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>gb|AE008692.1| Zymomonas mobilis subsp. mobilis ZM4, complete genome
          Length = 2056416

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 26/28 (92%)
 Strand = Plus / Minus

                                          
Query: 12     tatatttcaaaaaataaacgcatacaca 39
              ||||||||||||||| |||||| |||||
Sbjct: 339170 tatatttcaaaaaattaacgcagacaca 339143
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>emb|AL671881.8| Mouse DNA sequence from clone RP23-206E1 on chromosome X, complete
              sequence
          Length = 195413

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                  
Query: 4      tgtgcacatatatttcaaaa 23
              ||||||||||||||||||||
Sbjct: 179900 tgtgcacatatatttcaaaa 179881
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>dbj|AB020871.1| Homo sapiens genomic DNA of 9q32 anti-oncogene of flat epitherium
             cancer , segment 3/10
          Length = 100000

 Score = 40.1 bits (20), Expect = 5.8
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 406   ctgttgcttctttagccaaa 425
             ||||||||||||||||||||
Sbjct: 70780 ctgttgcttctttagccaaa 70761


  Database: nt
    Posted date:  May 29, 2006 11:10 AM
  Number of letters in database: 3,984,495,279
  Number of sequences in database:  917,343
  
  Database: /shigen/export/home/twatanab/db/nt/nt.01
    Posted date:  May 29, 2006 11:16 AM
  Number of letters in database: 3,988,174,986
  Number of sequences in database:  835,257
  
  Database: /shigen/export/home/twatanab/db/nt/nt.02
    Posted date:  May 29, 2006 11:21 AM
  Number of letters in database: 3,991,246,324
  Number of sequences in database:  771,481
  
  Database: /shigen/export/home/twatanab/db/nt/nt.03
    Posted date:  May 29, 2006 11:27 AM
  Number of letters in database: 3,990,718,311
  Number of sequences in database:  977,174
  
  Database: /shigen/export/home/twatanab/db/nt/nt.04
    Posted date:  May 29, 2006 11:29 AM
  Number of letters in database: 1,278,410,368
  Number of sequences in database:  400,813
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 4,395,785
Number of Sequences: 3902068
Number of extensions: 4395785
Number of successful extensions: 100393
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 32
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 100223
Number of HSP's gapped (non-prelim): 170
length of query: 430
length of database: 17,233,045,268
effective HSP length: 22
effective length of query: 408
effective length of database: 17,147,199,772
effective search space: 6996057506976
effective search space used: 6996057506976
T: 0
A: 0
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)
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