ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd22k20
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1YCH5_YEAST (P25601) Hypothetical transposon Ty5-1 protein YCL075W 38 0.022
2POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from tran... 36 0.11
3RAD52_NEUCR (Q9HGI2) DNA repair and recombination protein mus-11... 32 2.1
4SPRL1_RAT (P24054) SPARC-like protein 1 precursor (Matrix glycop... 30 6.0
5KLEG1_ECOLI (Q52282) Putative protein kleG 30 7.9
6ISPH_BACHK (Q6HDN0) 4-hydroxy-3-methylbut-2-enyl diphosphate red... 30 7.9
7ISPH_BACCZ (Q634Q0) 4-hydroxy-3-methylbut-2-enyl diphosphate red... 30 7.9
8ISPH_BACC1 (Q730P8) 4-hydroxy-3-methylbut-2-enyl diphosphate red... 30 7.9
9ISPH_BACAN (Q81LU9) 4-hydroxy-3-methylbut-2-enyl diphosphate red... 30 7.9
10APAG_BDEBA (Q6MK56) Protein apaG 30 7.9

>YCH5_YEAST (P25601) Hypothetical transposon Ty5-1 protein YCL075W|
          Length = 146

 Score = 38.1 bits (87), Expect = 0.022
 Identities = 23/65 (35%), Positives = 30/65 (46%)
 Frame = -2

Query: 634 TAATQHATGNRDLLSGLTPDQPGRFVLSADGAAPMQVVAHGCVITNTVVLPNVLYVPGLT 455
           T  T H   +R + S  T      FV    G+ P  ++  G V   TV L +V YVP L 
Sbjct: 83  TGCTSHMCHDRSIFSSFTRSSRKDFVRGVGGSIP--IMGSGTVNIGTVQLHDVSYVPDLP 140

Query: 454 ANLVS 440
            NL+S
Sbjct: 141 VNLIS 145



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>POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from transposon TNT|
           1-94 [Includes: Protease (EC 3.4.23.-); Reverse
           transcriptase (EC 2.7.7.49); Endonuclease]
          Length = 1328

 Score = 35.8 bits (81), Expect = 0.11
 Identities = 26/82 (31%), Positives = 37/82 (45%), Gaps = 3/82 (3%)
 Frame = -2

Query: 634 TAATQHATGNRDLLSGLTPDQPGRFVLSADGAAPMQVVAHGCVITN---TVVLPNVLYVP 464
           TAA+ HAT  RDL         G   +     + +  +   C+ TN   T+VL +V +VP
Sbjct: 298 TAASHHATPVRDLFCRYVAGDFGTVKMGNTSYSKIAGIGDICIKTNVGCTLVLKDVRHVP 357

Query: 463 GLTANLVSASQLVELNYTLEFS 398
            L  NL+S   L    Y   F+
Sbjct: 358 DLRMNLISGIALDRDGYESYFA 379



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>RAD52_NEUCR (Q9HGI2) DNA repair and recombination protein mus-11 (RAD52|
           homolog)
          Length = 600

 Score = 31.6 bits (70), Expect = 2.1
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = -1

Query: 620 ARDGEP*PPIRFNARSTGPLRSLGRRSGAHAGRRP 516
           AR+G+  PP +       P  SL R +GAHA  RP
Sbjct: 418 ARNGQHVPPAKTTETEAEPSTSLSRPAGAHAASRP 452



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>SPRL1_RAT (P24054) SPARC-like protein 1 precursor (Matrix glycoprotein Sc1)|
          Length = 634

 Score = 30.0 bits (66), Expect = 6.0
 Identities = 27/89 (30%), Positives = 36/89 (40%), Gaps = 7/89 (7%)
 Frame = +2

Query: 290 RQDFQEVQLEQTTFSNNTGFADDGAIGGTPDMTRTTAK-LEGVVELDKLTGRDE------ 448
           + DF++    Q   SN  G  +D A G    +  T  +  EG   LD +  R +      
Sbjct: 245 KNDFEQGSQGQEGDSNAEG--EDKAAGSKEHLPHTEWQGQEGRAGLDAIGNRKDTDEEKA 302

Query: 449 VSREPWDVQHVGENHRVCDDTAVGDDLHG 535
           VS EP D   V  NH   D+    D  HG
Sbjct: 303 VSTEPTDAAVVPRNHGASDNGGGDDSKHG 331



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>KLEG1_ECOLI (Q52282) Putative protein kleG|
          Length = 87

 Score = 29.6 bits (65), Expect = 7.9
 Identities = 9/20 (45%), Positives = 14/20 (70%)
 Frame = -2

Query: 178 AWACS*LVWPNAASYWACMP 119
           +W CS   WP++ + WAC+P
Sbjct: 12  SWPCSAPPWPSSRTGWACLP 31



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>ISPH_BACHK (Q6HDN0) 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC|
           1.17.1.2)
          Length = 316

 Score = 29.6 bits (65), Expect = 7.9
 Identities = 14/49 (28%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
 Frame = +2

Query: 356 DGAIGGTPDMTRTTAKLEGVVEL----DKLTGRDEVSREPWDVQHVGEN 490
           +GA+G  PD+     + + +  L    DK+   ++ +   WDVQH+ E+
Sbjct: 135 EGAVGIAPDIVHLIERADDLKTLEIPTDKILVTNQTTMSQWDVQHLMED 183



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>ISPH_BACCZ (Q634Q0) 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC|
           1.17.1.2)
          Length = 316

 Score = 29.6 bits (65), Expect = 7.9
 Identities = 14/49 (28%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
 Frame = +2

Query: 356 DGAIGGTPDMTRTTAKLEGVVEL----DKLTGRDEVSREPWDVQHVGEN 490
           +GA+G  PD+     + + +  L    DK+   ++ +   WDVQH+ E+
Sbjct: 135 EGAVGIAPDIVHLIERADDLKTLEIPTDKILVTNQTTMSQWDVQHLMED 183



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>ISPH_BACC1 (Q730P8) 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC|
           1.17.1.2)
          Length = 316

 Score = 29.6 bits (65), Expect = 7.9
 Identities = 14/49 (28%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
 Frame = +2

Query: 356 DGAIGGTPDMTRTTAKLEGVVEL----DKLTGRDEVSREPWDVQHVGEN 490
           +GA+G  PD+     + + +  L    DK+   ++ +   WDVQH+ E+
Sbjct: 135 EGAVGIAPDIVHLIERADDLKTLEIPTDKILVTNQTTMSQWDVQHLMED 183



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>ISPH_BACAN (Q81LU9) 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC|
           1.17.1.2)
          Length = 316

 Score = 29.6 bits (65), Expect = 7.9
 Identities = 14/49 (28%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
 Frame = +2

Query: 356 DGAIGGTPDMTRTTAKLEGVVEL----DKLTGRDEVSREPWDVQHVGEN 490
           +GA+G  PD+     + + +  L    DK+   ++ +   WDVQH+ E+
Sbjct: 135 EGAVGIAPDIVHLIERADDLKTLEIPTDKILVTNQTTMSQWDVQHLMED 183



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>APAG_BDEBA (Q6MK56) Protein apaG|
          Length = 127

 Score = 29.6 bits (65), Expect = 7.9
 Identities = 21/81 (25%), Positives = 41/81 (50%)
 Frame = -2

Query: 565 RFVLSADGAAPMQVVAHGCVITNTVVLPNVLYVPGLTANLVSASQLVELNYTLEFSRGAC 386
           +  ++  G+ P Q+++   VIT+ +     +  PG+    V     ++   T E+   AC
Sbjct: 34  KITITNTGSTPAQLMSRHWVITDALGKKEEVRGPGV----VGLQPKIQPGQTFEYD-SAC 88

Query: 385 HIRSAADGTIVGKASVVGESG 323
            + ++  G++VG+   VGESG
Sbjct: 89  PLTTST-GSMVGRYFFVGESG 108


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 94,139,881
Number of Sequences: 219361
Number of extensions: 2012798
Number of successful extensions: 4989
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4773
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4982
length of database: 80,573,946
effective HSP length: 107
effective length of database: 57,102,319
effective search space used: 5938641176
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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