ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd22e21
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1MA122_DROME (P53625) Mannosyl-oligosaccharide alpha-1,2-mannosid... 90 6e-18
2MA121_DROME (P53624) Mannosyl-oligosaccharide alpha-1,2-mannosid... 90 6e-18
3MA1A1_PIG (O02773) Mannosyl-oligosaccharide 1,2-alpha-mannosidas... 81 2e-15
4MA1C1_HUMAN (Q9NR34) Mannosyl-oligosaccharide 1,2-alpha-mannosid... 79 8e-15
5MA1A1_MOUSE (P45700) Mannosyl-oligosaccharide 1,2-alpha-mannosid... 78 2e-14
6MA1A1_RABIT (P45701) Mannosyl-oligosaccharide 1,2-alpha-mannosid... 78 2e-14
7MA1A2_MOUSE (P39098) Mannosyl-oligosaccharide 1,2-alpha-mannosid... 78 2e-14
8MA1A2_HUMAN (O60476) Mannosyl-oligosaccharide 1,2-alpha-mannosid... 78 2e-14
9MA1A1_HUMAN (P33908) Mannosyl-oligosaccharide 1,2-alpha-mannosid... 75 1e-13
10MA1B1_HUMAN (Q9UKM7) Endoplasmic reticulum mannosyl-oligosacchar... 68 2e-11
11EDEM3_HUMAN (Q9BZQ6) ER degradation-enhancing alpha-mannosidase-... 64 3e-10
12EDEM2_HUMAN (Q9BV94) ER degradation-enhancing alpha-mannosidase-... 63 6e-10
13YH04_YEAST (P38888) Protein YHR204W 57 5e-08
14EDEM1_MOUSE (Q925U4) ER degradation-enhancing alpha-mannosidase-... 56 8e-08
15EDEM1_HUMAN (Q92611) ER degradation-enhancing alpha-mannosidase-... 54 5e-07
16MNS1_CANAL (Q8J0Q0) Mannosyl-oligosaccharide 1,2-alpha-mannosida... 52 2e-06
17MNS1_YEAST (P32906) Endoplasmic reticulum mannosyl-oligosacchari... 49 9e-06
18YKU1_SCHPO (Q9P7C3) Putative mannosyl-oligosaccharide 1,2-alpha-... 49 2e-05
19MAN12_PENCI (P31723) Mannosyl-oligosaccharide alpha-1,2-mannosid... 46 1e-04

>MA122_DROME (P53625) Mannosyl-oligosaccharide alpha-1,2-mannosidase isoform 2|
           (EC 3.2.1.113) (Man(9)-alpha-mannosidase)
          Length = 643

 Score = 89.7 bits (221), Expect = 6e-18
 Identities = 49/74 (66%), Positives = 53/74 (71%), Gaps = 2/74 (2%)
 Frame = -3

Query: 619 AFEKNSRIASGYVGLRDVNSGE--KDDKMQSFFLAETLKYLYLLFSPPSVVSFDEWVFNT 446
           A EK+ R A GY GLR+V   E  KDD  QSFFLAETLKYLYLLFS  SV+  DEWVFNT
Sbjct: 557 ALEKHCRTAHGYCGLRNVYQQEPQKDDVQQSFFLAETLKYLYLLFSDDSVLPLDEWVFNT 616

Query: 445 EAHPLRIVPTHGSN 404
           EAHPL   P  G+N
Sbjct: 617 EAHPL---PIKGAN 627



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>MA121_DROME (P53624) Mannosyl-oligosaccharide alpha-1,2-mannosidase isoform 1|
           (EC 3.2.1.113) (Man(9)-alpha-mannosidase)
          Length = 667

 Score = 89.7 bits (221), Expect = 6e-18
 Identities = 49/74 (66%), Positives = 53/74 (71%), Gaps = 2/74 (2%)
 Frame = -3

Query: 619 AFEKNSRIASGYVGLRDVNSGE--KDDKMQSFFLAETLKYLYLLFSPPSVVSFDEWVFNT 446
           A EK+ R A GY GLR+V   E  KDD  QSFFLAETLKYLYLLFS  SV+  DEWVFNT
Sbjct: 581 ALEKHCRTAHGYCGLRNVYQQEPQKDDVQQSFFLAETLKYLYLLFSDDSVLPLDEWVFNT 640

Query: 445 EAHPLRIVPTHGSN 404
           EAHPL   P  G+N
Sbjct: 641 EAHPL---PIKGAN 651



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>MA1A1_PIG (O02773) Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (EC|
           3.2.1.113) (Processing alpha-1,2-mannosidase IA)
           (Alpha-1,2-mannosidase IA) (Mannosidase alpha class 1A
           member 1) (Man(9)-alpha-mannosidase) (Man9-mannosidase)
          Length = 659

 Score = 81.3 bits (199), Expect = 2e-15
 Identities = 39/69 (56%), Positives = 50/69 (72%), Gaps = 2/69 (2%)
 Frame = -3

Query: 622 EAFEKNSRIASGYVGLRDV--NSGEKDDKMQSFFLAETLKYLYLLFSPPSVVSFDEWVFN 449
           EA EK+ R+  GY GLRDV  ++   DD  QSFFLAETLKYLYL+FS   ++  + W+FN
Sbjct: 579 EALEKHCRVNGGYSGLRDVYVSAQTYDDVQQSFFLAETLKYLYLIFSDDDLLPLEHWIFN 638

Query: 448 TEAHPLRIV 422
           TEAHPL ++
Sbjct: 639 TEAHPLPVL 647



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>MA1C1_HUMAN (Q9NR34) Mannosyl-oligosaccharide 1,2-alpha-mannosidase IC (EC|
           3.2.1.113) (Processing alpha-1,2-mannosidase IC)
           (Alpha-1,2-mannosidase IC) (Mannosidase alpha class 1C
           member 1) (HMIC)
          Length = 630

 Score = 79.3 bits (194), Expect = 8e-15
 Identities = 39/67 (58%), Positives = 49/67 (73%), Gaps = 2/67 (2%)
 Frame = -3

Query: 619 AFEKNSRIASGYVGLRDVNSG--EKDDKMQSFFLAETLKYLYLLFSPPSVVSFDEWVFNT 446
           A EK  R  +G+ G++DV S     D+K QSFFLAETLKYLYLLFS   ++S ++WVFNT
Sbjct: 551 ALEKYCRTEAGFSGIQDVYSSTPNHDNKQQSFFLAETLKYLYLLFSEDDLLSLEDWVFNT 610

Query: 445 EAHPLRI 425
           EAHPL +
Sbjct: 611 EAHPLPV 617



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>MA1A1_MOUSE (P45700) Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (EC|
           3.2.1.113) (Processing alpha-1,2-mannosidase IA)
           (Alpha-1,2-mannosidase IA) (Mannosidase alpha class 1A
           member 1) (Man(9)-alpha-mannosidase) (Man9-mannosidase)
          Length = 655

 Score = 78.2 bits (191), Expect = 2e-14
 Identities = 38/69 (55%), Positives = 47/69 (68%), Gaps = 2/69 (2%)
 Frame = -3

Query: 622 EAFEKNSRIASGYVGLRDVNSGEK--DDKMQSFFLAETLKYLYLLFSPPSVVSFDEWVFN 449
           EA E + R+  GY GLRDV    +  DD  QSFFLAETLKYLYL+FS   ++  + W+FN
Sbjct: 575 EALESHCRVNGGYSGLRDVYIARESYDDVQQSFFLAETLKYLYLIFSDDDLLPLEHWIFN 634

Query: 448 TEAHPLRIV 422
           TEAHP  I+
Sbjct: 635 TEAHPFPIL 643



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>MA1A1_RABIT (P45701) Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (EC|
           3.2.1.113) (Processing alpha-1,2-mannosidase IA)
           (Alpha-1,2-mannosidase IA) (Mannosidase alpha class 1A
           member 1) (Man(9)-alpha-mannosidase) (Fragment)
          Length = 469

 Score = 78.2 bits (191), Expect = 2e-14
 Identities = 41/71 (57%), Positives = 51/71 (71%), Gaps = 2/71 (2%)
 Frame = -3

Query: 622 EAFEKNSRIASGYVGLRDVN-SGEK-DDKMQSFFLAETLKYLYLLFSPPSVVSFDEWVFN 449
           EA E + R+  GY GLRDV  + EK D+  QSFFLAETLKYLYL+FS   ++  + W+FN
Sbjct: 389 EALESHCRVNGGYSGLRDVYFTHEKYDNVQQSFFLAETLKYLYLIFSDDDLLPLEHWIFN 448

Query: 448 TEAHPLRIVPT 416
           TEAH L I+PT
Sbjct: 449 TEAHLLPILPT 459



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>MA1A2_MOUSE (P39098) Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB (EC|
           3.2.1.113) (Processing alpha-1,2-mannosidase IB)
           (Alpha-1,2-mannosidase IB) (Mannosidase alpha class 1A
           member 2)
          Length = 641

 Score = 78.2 bits (191), Expect = 2e-14
 Identities = 38/68 (55%), Positives = 49/68 (72%), Gaps = 2/68 (2%)
 Frame = -3

Query: 619 AFEKNSRIASGYVGLRDVNSGEK--DDKMQSFFLAETLKYLYLLFSPPSVVSFDEWVFNT 446
           A EK+ R++ G+ G++DV +     DD  QSFFLAETLKYLYLLFS   ++  D WVFNT
Sbjct: 560 AIEKSCRVSGGFSGVKDVYAPTPVHDDVQQSFFLAETLKYLYLLFSGDDLLPLDHWVFNT 619

Query: 445 EAHPLRIV 422
           EAHPL ++
Sbjct: 620 EAHPLPVL 627



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>MA1A2_HUMAN (O60476) Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB (EC|
           3.2.1.113) (Processing alpha-1,2-mannosidase IB)
           (Alpha-1,2-mannosidase IB) (Mannosidase alpha class 1A
           member 2)
          Length = 641

 Score = 77.8 bits (190), Expect = 2e-14
 Identities = 39/68 (57%), Positives = 47/68 (69%), Gaps = 2/68 (2%)
 Frame = -3

Query: 619 AFEKNSRIASGYVGLRDVNSGEK--DDKMQSFFLAETLKYLYLLFSPPSVVSFDEWVFNT 446
           A EK  R+  G+ G++DV S     DD  QSFFLAETLKYLYLLFS   ++  D WVFNT
Sbjct: 560 AIEKYCRVNGGFSGVKDVYSSTPTHDDVQQSFFLAETLKYLYLLFSGDDLLPLDHWVFNT 619

Query: 445 EAHPLRIV 422
           EAHPL ++
Sbjct: 620 EAHPLPVL 627



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>MA1A1_HUMAN (P33908) Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (EC|
           3.2.1.113) (Processing alpha-1,2-mannosidase IA)
           (Alpha-1,2-mannosidase IA) (Mannosidase alpha class 1A
           member 1) (Man(9)-alpha-mannosidase) (Man9-mannosidase)
          Length = 653

 Score = 75.5 bits (184), Expect = 1e-13
 Identities = 38/70 (54%), Positives = 48/70 (68%), Gaps = 2/70 (2%)
 Frame = -3

Query: 622 EAFEKNSRIASGYVGLRDVNSGEK--DDKMQSFFLAETLKYLYLLFSPPSVVSFDEWVFN 449
           EA E + R+  GY GLRDV    +  DD  QSFFLAETLKYLYL+FS   ++  + W+FN
Sbjct: 573 EALENHCRVNGGYSGLRDVYLLHESYDDVQQSFFLAETLKYLYLIFSDDDLLPLEHWIFN 632

Query: 448 TEAHPLRIVP 419
           +EAH L I+P
Sbjct: 633 SEAHLLPILP 642



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>MA1B1_HUMAN (Q9UKM7) Endoplasmic reticulum mannosyl-oligosaccharide|
           1,2-alpha-mannosidase (EC 3.2.1.113) (ER
           alpha-1,2-mannosidase) (Mannosidase alpha class 1B
           member 1) (Man9GlcNAc2-specific-processing
           alpha-mannosidase)
          Length = 699

 Score = 68.2 bits (165), Expect = 2e-11
 Identities = 37/73 (50%), Positives = 52/73 (71%), Gaps = 5/73 (6%)
 Frame = -3

Query: 628 VKEAFEKNSRIASG-YVGLRDVNSGEKD---DKMQSFFLAETLKYLYLLFS-PPSVVSFD 464
           + ++F + +R+ SG Y  + +V   +K    DKM+SFFL ETLKYL+LLFS  P+++S D
Sbjct: 623 ILQSFSRFTRVPSGGYSSINNVQDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSLD 682

Query: 463 EWVFNTEAHPLRI 425
            +VFNTEAHPL I
Sbjct: 683 AYVFNTEAHPLPI 695



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>EDEM3_HUMAN (Q9BZQ6) ER degradation-enhancing alpha-mannosidase-like 3|
          Length = 889

 Score = 64.3 bits (155), Expect = 3e-10
 Identities = 33/76 (43%), Positives = 48/76 (63%), Gaps = 4/76 (5%)
 Frame = -3

Query: 622 EAFEKNSRIASGYVGLRDVNSGEKDDKMQSFFLAETLKYLYLLFSPPSVVSFD--EWVFN 449
           E   K +R+  G+  ++DV +G  +D+M SFFLAE  KYLYLLF+    + FD  +++F 
Sbjct: 388 ENLNKYARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLFADKEDIIFDIEDYIFT 447

Query: 448 TEAH--PLRIVPTHGS 407
           TEAH  PL +  T+ S
Sbjct: 448 TEAHLLPLWLSTTNQS 463



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>EDEM2_HUMAN (Q9BV94) ER degradation-enhancing alpha-mannosidase-like 2|
           precursor
          Length = 578

 Score = 63.2 bits (152), Expect = 6e-10
 Identities = 33/89 (37%), Positives = 51/89 (57%), Gaps = 19/89 (21%)
 Frame = -3

Query: 622 EAFEKNSRIASGYVGLRDVNSGEKDDKMQSFFLAETLKYLYLLFSPPSVV-----SFDE- 461
           E+ EK S++  G+  ++D+   + D++M+SFFLAET+KYLYLLF P + +     +FD  
Sbjct: 398 ESIEKISKVECGFATIKDLRDHKLDNRMESFFLAETVKYLYLLFDPTNFIHNNGSTFDAV 457

Query: 460 -------------WVFNTEAHPLRIVPTH 413
                        ++FNTEAHP+     H
Sbjct: 458 ITPYGECILGAGGYIFNTEAHPIDPAALH 486



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>YH04_YEAST (P38888) Protein YHR204W|
          Length = 796

 Score = 57.0 bits (136), Expect = 5e-08
 Identities = 29/68 (42%), Positives = 46/68 (67%), Gaps = 2/68 (2%)
 Frame = -3

Query: 628 VKEAFEKNSRIASGYVGLRDVNSGEKDDKMQSFFLAETLKYLYLLFSPPSVV--SFDEWV 455
           +K+ F+ N     G+ G ++V +GE  D+M++F L+ETLKYLYLLF   + +  S  + +
Sbjct: 437 LKQRFKSNC----GFAGFQNVITGELQDRMETFVLSETLKYLYLLFDEENELHNSASDVI 492

Query: 454 FNTEAHPL 431
           F+TEAHP+
Sbjct: 493 FSTEAHPM 500



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>EDEM1_MOUSE (Q925U4) ER degradation-enhancing alpha-mannosidase-like 1|
          Length = 652

 Score = 56.2 bits (134), Expect = 8e-08
 Identities = 28/70 (40%), Positives = 43/70 (61%), Gaps = 2/70 (2%)
 Frame = -3

Query: 628 VKEAFEKNSRIASGYVGLRDVNSGEKDDKMQSFFLAETLKYLYLLFSPPSVV--SFDEWV 455
           + ++ EK +++  GY  L  V    K+D+M+SFFL+ET KYLYLLF   + V  S   ++
Sbjct: 512 ILQSLEKYTKVKCGYATLHHVIDKSKEDRMESFFLSETCKYLYLLFDEENPVHKSGTRYM 571

Query: 454 FNTEAHPLRI 425
           F TE H + +
Sbjct: 572 FTTEGHIISV 581



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>EDEM1_HUMAN (Q92611) ER degradation-enhancing alpha-mannosidase-like 1|
          Length = 657

 Score = 53.5 bits (127), Expect = 5e-07
 Identities = 27/66 (40%), Positives = 40/66 (60%), Gaps = 2/66 (3%)
 Frame = -3

Query: 628 VKEAFEKNSRIASGYVGLRDVNSGEKDDKMQSFFLAETLKYLYLLFSPPSVV--SFDEWV 455
           + ++ EK +++  GY  L  V     +D+M+SFFL+ET KYLYLLF   + V  S   ++
Sbjct: 517 ILQSLEKYTKVKCGYATLHHVIDKSTEDRMESFFLSETCKYLYLLFDEDNPVHKSGTRYM 576

Query: 454 FNTEAH 437
           F TE H
Sbjct: 577 FTTEGH 582



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>MNS1_CANAL (Q8J0Q0) Mannosyl-oligosaccharide 1,2-alpha-mannosidase (EC|
           3.2.1.113) (Man(9)-alpha-mannosidase)
          Length = 565

 Score = 51.6 bits (122), Expect = 2e-06
 Identities = 28/64 (43%), Positives = 37/64 (57%), Gaps = 6/64 (9%)
 Frame = -3

Query: 607 NSRIASGYVGLRDVNSGEKD------DKMQSFFLAETLKYLYLLFSPPSVVSFDEWVFNT 446
           NS     +  L DV S + +      D  +SF+ AETLKYLYLLF   + +   ++VFNT
Sbjct: 442 NSEGEVSFSSLSDVTSFDSNGLPKFKDNTESFWWAETLKYLYLLFDDTNKIPLTDYVFNT 501

Query: 445 EAHP 434
           EAHP
Sbjct: 502 EAHP 505



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>MNS1_YEAST (P32906) Endoplasmic reticulum mannosyl-oligosaccharide|
           1,2-alpha-mannosidase (EC 3.2.1.113) (ER
           alpha-1,2-mannosidase) (Man(9)-alpha-mannosidase)
          Length = 549

 Score = 49.3 bits (116), Expect = 9e-06
 Identities = 28/59 (47%), Positives = 38/59 (64%), Gaps = 2/59 (3%)
 Frame = -3

Query: 556 EKDDKMQSFFLAETLKYLYLLFSPPSVVSFD--EWVFNTEAHPLRIVPTHGSNGQSIET 386
           +K + M+SF+LAETLKYLY+LF    +  FD  + VFNTEAHP  ++       QS+ T
Sbjct: 491 KKSNNMESFWLAETLKYLYILF----LDEFDLTKVVFNTEAHPFPVLDEEILKSQSLTT 545



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>YKU1_SCHPO (Q9P7C3) Putative mannosyl-oligosaccharide 1,2-alpha-mannosidase|
           (EC 3.2.1.113) (Man(9)-alpha-mannosidase)
          Length = 521

 Score = 48.5 bits (114), Expect = 2e-05
 Identities = 23/42 (54%), Positives = 30/42 (71%), Gaps = 1/42 (2%)
 Frame = -3

Query: 556 EKDDKMQSFFLAETLKYLYLLFSPP-SVVSFDEWVFNTEAHP 434
           ++ DK +SF+ AETLKYLYLLF    S++    + FNTEAHP
Sbjct: 467 DQRDKTESFWFAETLKYLYLLFEDDFSILPLTNYTFNTEAHP 508



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>MAN12_PENCI (P31723) Mannosyl-oligosaccharide alpha-1,2-mannosidase precursor|
           (EC 3.2.1.113) (Man(9)-alpha-mannosidase)
          Length = 511

 Score = 45.8 bits (107), Expect = 1e-04
 Identities = 26/73 (35%), Positives = 36/73 (49%), Gaps = 8/73 (10%)
 Frame = -3

Query: 619 AFEKNSRIASGYVGLRDVNS---GEKDDKMQSFFLAETLKYLYLLFSPPSVVSFDE---- 461
           A     R  SG+  + DVN    G K D  +SF  AE +KY YL  S  +     +    
Sbjct: 436 AINSTCRTDSGFAAVSDVNKANGGSKYDNQESFLFAEVMKYSYLAHSEDAAWQVQKGGKN 495

Query: 460 -WVFNTEAHPLRI 425
            +V+NTEAHP+ +
Sbjct: 496 TFVYNTEAHPISV 508


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,903,529
Number of Sequences: 219361
Number of extensions: 1669835
Number of successful extensions: 3343
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 3260
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3331
length of database: 80,573,946
effective HSP length: 107
effective length of database: 57,102,319
effective search space used: 5824436538
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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