ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd21m23
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1RF1_ORYSA (Q76C99) Rf1 protein, mitochondrial precursor (PPR pro... 53 6e-07
2Y4791_ARATH (P0C043) Hypothetical PPR-repeat protein At4g17915 34 0.28
3GCSP_SYNEL (Q8DII3) Glycine dehydrogenase [decarboxylating] (EC ... 32 0.82
4Y1991_PASMU (Q9CJL2) Hypothetical zinc metalloprotease PM1991 (E... 30 3.1
5SOX14_DROME (P40656) Putative transcription factor SOX-14 30 3.1
6PKHA4_HUMAN (Q9H4M7) Pleckstrin homology domain-containing famil... 30 4.1
7UNC52_CAEEL (Q06561) Basement membrane proteoglycan precursor (P... 30 5.3
8SALM_DROME (P39770) Homeotic protein spalt-major 29 7.0
9NOL6_HUMAN (Q9H6R4) Nucleolar protein 6 (Nucleolar RNA-associate... 29 7.0
10GLSA_SALTY (Q8ZPI2) Glutaminase (EC 3.5.1.2) 29 7.0
11BAR1_CHITE (P02849) Balbiani ring protein 1 (Giant secretory pro... 29 7.0
12LEU1_AQUAE (O67862) 2-isopropylmalate synthase (EC 2.3.3.13) (Al... 29 7.0
13CLCC_ARATH (Q96282) Chloride channel protein CLC-c (AtCLC-c) 29 9.1
14ZNF80_CERAE (P51502) Zinc finger protein 80 29 9.1
15CH60_CLOTM (P48212) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 29 9.1

>RF1_ORYSA (Q76C99) Rf1 protein, mitochondrial precursor (PPR protein)|
           (Fertility restorer) (Restorer for CMS)
          Length = 791

 Score = 52.8 bits (125), Expect = 6e-07
 Identities = 37/148 (25%), Positives = 66/148 (44%), Gaps = 7/148 (4%)
 Frame = -1

Query: 484 VVAAGHEPVSYTYCLMAQALARGGEVDAAVAILDDMVRKGYALRKRAYTDVVRALCDRSR 305
           +V  G  P  Y + ++  A A+ G+VD A+ +   M ++G       Y  V+  LC   R
Sbjct: 362 MVRNGIHPDHYVFSILICAYAKQGKVDQAMLVFSKMRQQGLNPNAVTYGAVIGILCKSGR 421

Query: 304 TYDALRVLAVVIAKDFVPGRNAFDALLEELSRQGRWPDAMAVYAAAVKRGVVVS------ 143
             DA+     +I +   PG   +++L+  L    +W  A  +    + RG+ ++      
Sbjct: 422 VEDAMLYFEQMIDEGLSPGNIVYNSLIHGLCTCNKWERAEELILEMLDRGICLNTIFFNS 481

Query: 142 -LKRHVKEALARDQEKEEAWESPAQLGV 62
            +  H KE   R  E E+ +E   ++GV
Sbjct: 482 IIDSHCKE--GRVIESEKLFELMVRIGV 507



 Score = 44.7 bits (104), Expect = 2e-04
 Identities = 28/107 (26%), Positives = 56/107 (52%), Gaps = 4/107 (3%)
 Frame = -1

Query: 493 FGKVVAAGHEPVS---YTYCLMAQALARGGEVDAAVAILDDMVRKGYALRKRAYTDVVRA 323
           + ++  AG + V+    TY ++     R G +D   A L ++++KG+ +   A+T +++ 
Sbjct: 72  YNRMARAGADEVTPDLCTYGILIGCCCRAGRLDLGFAALGNVIKKGFRVDAIAFTPLLKG 131

Query: 322 LCDRSRTYDALR-VLAVVIAKDFVPGRNAFDALLEELSRQGRWPDAM 185
           LC   RT DA+  VL  +     +P   +++ LL+ L  + R  +A+
Sbjct: 132 LCADKRTSDAMDIVLRRMTELGCIPNVFSYNILLKGLCDENRSQEAL 178



 Score = 43.9 bits (102), Expect = 3e-04
 Identities = 29/125 (23%), Positives = 56/125 (44%)
 Frame = -1

Query: 523 ISVLREAKEVFGKVVAAGHEPVSYTYCLMAQALARGGEVDAAVAILDDMVRKGYALRKRA 344
           IS + +A  +F ++ ++G  P   TY ++ Q L +     AA  +   +   G  +    
Sbjct: 559 ISRMEDALVLFKEMESSGVSPDIITYNIILQGLFQTRRTAAAKELYVRITESGTQIELST 618

Query: 343 YTDVVRALCDRSRTYDALRVLAVVIAKDFVPGRNAFDALLEELSRQGRWPDAMAVYAAAV 164
           Y  ++  LC    T DAL++   +   D       F+ +++ L + GR  +A  ++ A  
Sbjct: 619 YNIILHGLCKNKLTDDALQMFQNLCLMDLKLEARTFNIMIDALLKVGRNDEAKDLFVAFS 678

Query: 163 KRGVV 149
             G+V
Sbjct: 679 SNGLV 683



 Score = 42.7 bits (99), Expect = 6e-04
 Identities = 28/119 (23%), Positives = 53/119 (44%)
 Frame = -1

Query: 508 EAKEVFGKVVAAGHEPVSYTYCLMAQALARGGEVDAAVAILDDMVRKGYALRKRAYTDVV 329
           EA+++F  +   G +P   TY  + Q  A  G +     +LD MVR G       ++ ++
Sbjct: 319 EARKIFDSMTKRGLKPEITTYGTLLQGYATKGALVEMHGLLDLMVRNGIHPDHYVFSILI 378

Query: 328 RALCDRSRTYDALRVLAVVIAKDFVPGRNAFDALLEELSRQGRWPDAMAVYAAAVKRGV 152
            A   + +   A+ V + +  +   P    + A++  L + GR  DAM  +   +  G+
Sbjct: 379 CAYAKQGKVDQAMLVFSKMRQQGLNPNAVTYGAVIGILCKSGRVEDAMLYFEQMIDEGL 437



 Score = 40.8 bits (94), Expect = 0.002
 Identities = 24/119 (20%), Positives = 56/119 (47%)
 Frame = -1

Query: 508 EAKEVFGKVVAAGHEPVSYTYCLMAQALARGGEVDAAVAILDDMVRKGYALRKRAYTDVV 329
           +A  VF K+   G  P + TY  +   L + G V+ A+   + M+ +G +     Y  ++
Sbjct: 389 QAMLVFSKMRQQGLNPNAVTYGAVIGILCKSGRVEDAMLYFEQMIDEGLSPGNIVYNSLI 448

Query: 328 RALCDRSRTYDALRVLAVVIAKDFVPGRNAFDALLEELSRQGRWPDAMAVYAAAVKRGV 152
             LC  ++   A  ++  ++ +        F+++++   ++GR  ++  ++   V+ GV
Sbjct: 449 HGLCTCNKWERAEELILEMLDRGICLNTIFFNSIIDSHCKEGRVIESEKLFELMVRIGV 507



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>Y4791_ARATH (P0C043) Hypothetical PPR-repeat protein At4g17915|
          Length = 463

 Score = 33.9 bits (76), Expect = 0.28
 Identities = 25/117 (21%), Positives = 51/117 (43%)
 Frame = -1

Query: 505 AKEVFGKVVAAGHEPVSYTYCLMAQALARGGEVDAAVAILDDMVRKGYALRKRAYTDVVR 326
           A E+F K + +  +P   TY ++   L +   V  A  +L ++ + GY      YT +++
Sbjct: 173 ALELF-KEMQSRFKPELMTYNILINGLCKSRRVGTAKWMLTELKKSGYTPNAVTYTTILK 231

Query: 325 ALCDRSRTYDALRVLAVVIAKDFVPGRNAFDALLEELSRQGRWPDAMAVYAAAVKRG 155
                 R    L++   +  + +     A+ A++  L + GR  +A       V++G
Sbjct: 232 LYFKTRRIRRGLQLFLEMKREGYTYDGYAYFAVVSALIKTGRTKEAYEYMQELVRKG 288



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>GCSP_SYNEL (Q8DII3) Glycine dehydrogenase [decarboxylating] (EC 1.4.4.2)|
           (Glycine decarboxylase) (Glycine cleavage system
           P-protein)
          Length = 954

 Score = 32.3 bits (72), Expect = 0.82
 Identities = 30/110 (27%), Positives = 46/110 (41%), Gaps = 9/110 (8%)
 Frame = -1

Query: 442 LMAQALARGGEVDAAVAILDDMVRKGYALRKRAYTDVVRALCDRSRTYDALRVLAVVIAK 263
           L   A+A    +D A A  + M     A R+R     + A     +T   LR  A+ +  
Sbjct: 142 LTGLAIANASLLDEATAAAEAMTLSFNACRQRGANRFLVAQDCHPQTLAVLRTRALPLGI 201

Query: 262 DFVP---------GRNAFDALLEELSRQGRWPDAMAVYAAAVKRGVVVSL 140
             VP           NAF  LL+  +  G      A+ AAA +RG++V++
Sbjct: 202 QIVPIDPIAGELPWENAFGLLLQYPASDGAVRSPQALIAAAHERGLLVTV 251



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>Y1991_PASMU (Q9CJL2) Hypothetical zinc metalloprotease PM1991 (EC 3.4.24.-)|
          Length = 442

 Score = 30.4 bits (67), Expect = 3.1
 Identities = 18/51 (35%), Positives = 27/51 (52%)
 Frame = -1

Query: 250 GRNAFDALLEELSRQGRWPDAMAVYAAAVKRGVVVSLKRHVKEALARDQEK 98
           G+ +F  L  EL+++GRW   +A  AA +       LK  + EAL +  EK
Sbjct: 275 GQFSFVVLTPELNKKGRWYVGIAPTAAPISDIYRTELKYGILEALQKGVEK 325



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>SOX14_DROME (P40656) Putative transcription factor SOX-14|
          Length = 472

 Score = 30.4 bits (67), Expect = 3.1
 Identities = 23/68 (33%), Positives = 29/68 (42%), Gaps = 2/68 (2%)
 Frame = +2

Query: 131 VTLQRHNDAPLHRSGVHC-HGVGPSPLPAELFEQRVERVPAGHEVLRDHHGQHPESVVSP 307
           +T+QR + AP   S  H      PSP PA        + P   +        HP +V SP
Sbjct: 35  ITIQRRHPAPKADSTPHTLPPFSPSPSPASSPSPAPAQTPGAQKTQSQAAITHPAAVASP 94

Query: 308 -APVAQRA 328
            APVA  A
Sbjct: 95  SAPVAAAA 102



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>PKHA4_HUMAN (Q9H4M7) Pleckstrin homology domain-containing family A member 4|
           (Phosphoinositol 3-phosphate-binding protein 1) (PEPP-1)
          Length = 779

 Score = 30.0 bits (66), Expect = 4.1
 Identities = 12/22 (54%), Positives = 15/22 (68%)
 Frame = -2

Query: 249 AGTRSTRCSKSSAGRGDGPTPW 184
           A + ST  S+  +GRG GPTPW
Sbjct: 727 ANSGSTGFSRRGSGRGGGPTPW 748



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>UNC52_CAEEL (Q06561) Basement membrane proteoglycan precursor (Perlecan homolog)|
            (Uncoordinated protein 52)
          Length = 3375

 Score = 29.6 bits (65), Expect = 5.3
 Identities = 14/34 (41%), Positives = 16/34 (47%)
 Frame = -2

Query: 246  GTRSTRCSKSSAGRGDGPTPWQCTPLR*SGASLC 145
            GT   RC K   G     +P+ CTP    GAS C
Sbjct: 1036 GTHCERCKKGYYGDATKGSPYDCTPCPCPGASDC 1069



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>SALM_DROME (P39770) Homeotic protein spalt-major|
          Length = 1365

 Score = 29.3 bits (64), Expect = 7.0
 Identities = 17/53 (32%), Positives = 24/53 (45%)
 Frame = +2

Query: 182 CHGVGPSPLPAELFEQRVERVPAGHEVLRDHHGQHPESVVSPAPVAQRAHHVR 340
           C    P PLP E+   + ERV    +V ++ H   P    SP+   +  HH R
Sbjct: 626 CSEPEPEPLPLEV-RIKEERVEEQEQVKQEDHRIEPRRTPSPSSEHRSPHHHR 677



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>NOL6_HUMAN (Q9H6R4) Nucleolar protein 6 (Nucleolar RNA-associated protein)|
            (Nrap)
          Length = 1146

 Score = 29.3 bits (64), Expect = 7.0
 Identities = 27/91 (29%), Positives = 34/91 (37%), Gaps = 5/91 (5%)
 Frame = +2

Query: 179  HCHGVGPSPLPAELFEQRVERVPAGH-----EVLRDHHGQHPESVVSPAPVAQRAHHVRV 343
            H  G G  P  AE     V+RV A       E+L   HG    +  +   V +     R+
Sbjct: 740  HLEGSGQWPQDAEA----VQRVRAAFQLRLAELLTQQHGLQCRATATHTDVLKDGFVFRI 795

Query: 344  RALPQRVPLAHHVVQYGHGRVHLAAACQSLR 436
            R   QR P     VQ   G + L     SLR
Sbjct: 796  RVAYQREPQILKEVQSPEGMISLRDTAASLR 826



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>GLSA_SALTY (Q8ZPI2) Glutaminase (EC 3.5.1.2)|
          Length = 308

 Score = 29.3 bits (64), Expect = 7.0
 Identities = 17/50 (34%), Positives = 28/50 (56%), Gaps = 1/50 (2%)
 Frame = -1

Query: 439 MAQALARGGEVDAAVAILDDMVRKGYALRKRAYTDVVRALCDRSR-TYDA 293
           M Q + R   ++A   ++ DM++   +  ++   +VVRALC  S  TYDA
Sbjct: 106 MEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRALCGVSDITYDA 155



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>BAR1_CHITE (P02849) Balbiani ring protein 1 (Giant secretory protein I-A)|
           (GSP-IA) (Fragment)
          Length = 174

 Score = 29.3 bits (64), Expect = 7.0
 Identities = 20/60 (33%), Positives = 27/60 (45%)
 Frame = -2

Query: 369 RGTRCGSARTRTWCARCATGAGLTTLSGCWPW*SRRTSCPAGTRSTRCSKSSAGRGDGPT 190
           R  RCGSA  +    +CA   G    S C     R TS    +R++  SK S  R + P+
Sbjct: 1   RPERCGSAMRKAEAEKCARRNGRFNASKC-----RCTSAGKPSRNSEPSKGSKPRPEKPS 55



 Score = 28.9 bits (63), Expect = 9.1
 Identities = 20/60 (33%), Positives = 26/60 (43%)
 Frame = -2

Query: 369 RGTRCGSARTRTWCARCATGAGLTTLSGCWPW*SRRTSCPAGTRSTRCSKSSAGRGDGPT 190
           R  RCGSA  +    +CA   G    S C     R TS    +R +  SK S  R + P+
Sbjct: 83  RPERCGSAMRKAEAEKCARRNGRFNASKC-----RCTSAGKPSRKSEPSKGSKPRPEKPS 137



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>LEU1_AQUAE (O67862) 2-isopropylmalate synthase (EC 2.3.3.13)|
           (Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
          Length = 520

 Score = 29.3 bits (64), Expect = 7.0
 Identities = 15/46 (32%), Positives = 21/46 (45%)
 Frame = +2

Query: 149 NDAPLHRSGVHCHGVGPSPLPAELFEQRVERVPAGHEVLRDHHGQH 286
           ++A  H SG+H HGV    +  E+        P    VL  H G+H
Sbjct: 291 DNAFAHESGIHQHGVLSHRMTYEIMNPEDVGFPMSRIVLGKHSGRH 336



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>CLCC_ARATH (Q96282) Chloride channel protein CLC-c (AtCLC-c)|
          Length = 779

 Score = 28.9 bits (63), Expect = 9.1
 Identities = 17/48 (35%), Positives = 22/48 (45%), Gaps = 4/48 (8%)
 Frame = -2

Query: 303 LTTLSGCW----PW*SRRTSCPAGTRSTRCSKSSAGRGDGPTPWQCTP 172
           ++ LS C     PW S+ T CP G    +C   S GR      +QC P
Sbjct: 386 VSILSSCCAFGLPWLSQCTPCPIGIEEGKC--PSVGRSSIYKSFQCPP 431



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>ZNF80_CERAE (P51502) Zinc finger protein 80|
          Length = 213

 Score = 28.9 bits (63), Expect = 9.1
 Identities = 21/89 (23%), Positives = 36/89 (40%)
 Frame = +2

Query: 221 FEQRVERVPAGHEVLRDHHGQHPESVVSPAPVAQRAHHVRVRALPQRVPLAHHVVQYGHG 400
           F ++V+ VP     +R H G+ P   V    V  R  H+          L HH +  G  
Sbjct: 107 FHEKVDFVPH----MRIHTGEKPCKCVECGKVFNRRSHL----------LCHHRIHTGEK 152

Query: 401 RVHLAAACQSLRHQAVRVRHRLVPGGHDL 487
               +   ++  + +V ++HR+   G  L
Sbjct: 153 PYECSECGKTFSYHSVFIQHRMTHTGEKL 181



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>CH60_CLOTM (P48212) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (HSP-60)|
          Length = 540

 Score = 28.9 bits (63), Expect = 9.1
 Identities = 21/83 (25%), Positives = 41/83 (49%)
 Frame = -1

Query: 451 TYCLMAQALARGGEVDAAVAILDDMVRKGYALRKRAYTDVVRALCDRSRTYDALRVLAVV 272
           T  L+AQA+ R G  + A      +++KG A    A  + ++ +  + +  + +  +A +
Sbjct: 89  TATLLAQAIIREGLKNVAAGANPMLLKKGIAKAVDAAVEGIKEISQKVKGKEDIARVASI 148

Query: 271 IAKDFVPGRNAFDALLEELSRQG 203
            A D V G    DA +E+++  G
Sbjct: 149 SANDEVIGELIADA-MEKVTNDG 170


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 62,573,521
Number of Sequences: 219361
Number of extensions: 1114334
Number of successful extensions: 4878
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 4717
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4866
length of database: 80,573,946
effective HSP length: 105
effective length of database: 57,541,041
effective search space used: 4027872870
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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