Clone Information

BLAST Search Result

Clone Name	rbasd21i03
Clone Library Name	barley_pub

No.	Definition	Score (bits)	E Value
1	CO5A1_MOUSE (O88207) Collagen alpha-1(V) chain precursor	32	1.1
2	CO5A1_HUMAN (P20908) Collagen alpha-1(V) chain precursor	32	1.1
3	COBA1_RAT (P20909) Collagen alpha-1(XI) chain precursor (Fragment)	32	1.1
4	COBA1_MOUSE (Q61245) Collagen alpha-1(XI) chain precursor	32	1.1
5	CO5A1_RAT (Q9JI03) Collagen alpha-1(V) chain precursor	32	1.1
6	CO5A1_CRILO (Q60467) Collagen alpha-1(V) chain precursor	32	1.1
7	COHA1_CHICK (Q90584) Collagen alpha-1(XVII) chain (Bullous pemph...	32	1.4
8	COBA1_HUMAN (P12107) Collagen alpha-1(XI) chain precursor	32	1.9
9	COL14_CAEEL (P18834) Cuticle collagen 14	32	1.9
10	RADA_NANEQ (Q74MX9) DNA repair and recombination protein radA	31	2.4
11	CO5A3_HUMAN (P25940) Collagen alpha-3(V) chain precursor	31	3.2
12	CO4A4_RABIT (P55787) Collagen alpha-4(IV) chain (Fragment)	31	3.2
13	CO1A2_HUMAN (P08123) Collagen alpha-2(I) chain precursor	30	4.2
14	COL8_CAEEL (P18833) Cuticle collagen 8 precursor	30	5.4
15	CO1A2_CHICK (P02467) Collagen alpha-2(I) chain precursor (Fragme...	30	5.4
16	AROK_METTH (O26896) Shikimate kinase (EC 2.7.1.71) (SK)	30	5.4
17	CO3A1_BOVIN (P04258) Collagen alpha-1(III) chain	30	7.1
18	HLYS_HYDAT (Q52SK7) Hydralysin	30	7.1
19	C1QC_MOUSE (Q02105) Complement C1q subcomponent subunit C precursor	30	7.1
20	CO7A1_HUMAN (Q02388) Collagen alpha-1(VII) chain precursor (Long...	29	9.3
21	HLDD_NEIMB (Q9K002) ADP-L-glycero-D-manno-heptose-6-epimerase (E...	29	9.3
22	HLDD_NEIGO (Q51061) ADP-L-glycero-D-manno-heptose-6-epimerase (E...	29	9.3
23	COIA1_MOUSE (P39061) Collagen alpha-1(XVIII) chain precursor [Co...	29	9.3
24	COL19_CAEEL (P18835) Cuticle collagen 19 precursor	29	9.3
25	CLPB_AQUAE (O67588) Chaperone clpB	29	9.3

>CO5A1_MOUSE (O88207) Collagen alpha-1(V) chain precursor|
          Length = 1838

 Score = 32.3 bits (72), Expect = 1.1
 Identities = 13/25 (52%), Positives = 17/25 (68%)
 Frame = +2

Query: 266  VPLPVGEGDVDGAPGRDGHDGDLGP 340
            +P PVGE  + G+PG DG  G +GP
Sbjct: 1442 IPGPVGEQGLPGSPGPDGPPGPMGP 1466

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>CO5A1_HUMAN (P20908) Collagen alpha-1(V) chain precursor|
          Length = 1838

 Score = 32.3 bits (72), Expect = 1.1
 Identities = 13/25 (52%), Positives = 17/25 (68%)
 Frame = +2

Query: 266  VPLPVGEGDVDGAPGRDGHDGDLGP 340
            +P PVGE  + G+PG DG  G +GP
Sbjct: 1442 IPGPVGEQGLPGSPGPDGPPGPMGP 1466

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>COBA1_RAT (P20909) Collagen alpha-1(XI) chain precursor (Fragment)|
          Length = 482

 Score = 32.3 bits (72), Expect = 1.1
 Identities = 14/25 (56%), Positives = 17/25 (68%)
 Frame = +2

Query: 266 VPLPVGEGDVDGAPGRDGHDGDLGP 340
           +P PVGE  + GA G+DG  G LGP
Sbjct: 88  IPGPVGEQGLPGAAGQDGPPGPLGP 112

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>COBA1_MOUSE (Q61245) Collagen alpha-1(XI) chain precursor|
          Length = 1804

 Score = 32.3 bits (72), Expect = 1.1
 Identities = 14/25 (56%), Positives = 17/25 (68%)
 Frame = +2

Query: 266  VPLPVGEGDVDGAPGRDGHDGDLGP 340
            +P PVGE  + GA G+DG  G LGP
Sbjct: 1410 IPGPVGEQGLPGAAGQDGPPGPLGP 1434



 Score = 29.3 bits (64), Expect = 9.3
 Identities = 11/25 (44%), Positives = 16/25 (64%)
 Frame = +2

Query: 266  VPLPVGEGDVDGAPGRDGHDGDLGP 340
            +P P+G   + G PG  G +GD+GP
Sbjct: 1191 LPGPIGLQGLPGPPGEKGENGDVGP 1215

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>CO5A1_RAT (Q9JI03) Collagen alpha-1(V) chain precursor|
          Length = 1840

 Score = 32.3 bits (72), Expect = 1.1
 Identities = 13/25 (52%), Positives = 17/25 (68%)
 Frame = +2

Query: 266  VPLPVGEGDVDGAPGRDGHDGDLGP 340
            +P PVGE  + G+PG DG  G +GP
Sbjct: 1444 IPGPVGEQGLPGSPGPDGPPGPMGP 1468

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>CO5A1_CRILO (Q60467) Collagen alpha-1(V) chain precursor|
          Length = 1840

 Score = 32.3 bits (72), Expect = 1.1
 Identities = 13/25 (52%), Positives = 17/25 (68%)
 Frame = +2

Query: 266  VPLPVGEGDVDGAPGRDGHDGDLGP 340
            +P PVGE  + G+PG DG  G +GP
Sbjct: 1444 IPGPVGEQGLPGSPGPDGPPGPMGP 1468

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>COHA1_CHICK (Q90584) Collagen alpha-1(XVII) chain (Bullous pemphigoid antigen 2)|
            (180 kDa bullous pemphigoid antigen 2) (Fragment)
          Length = 1146

 Score = 32.0 bits (71), Expect = 1.4
 Identities = 15/31 (48%), Positives = 17/31 (54%)
 Frame = +2

Query: 257  YGDVPLPVGEGDVDGAPGRDGHDGDLGPGRH 349
            +G V  P GE    G PG  G  G +GPGRH
Sbjct: 1053 HGTVRGPPGEKGERGYPGPKGDPGPMGPGRH 1083

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>COBA1_HUMAN (P12107) Collagen alpha-1(XI) chain precursor|
          Length = 1806

 Score = 31.6 bits (70), Expect = 1.9
 Identities = 13/25 (52%), Positives = 17/25 (68%)
 Frame = +2

Query: 266  VPLPVGEGDVDGAPGRDGHDGDLGP 340
            +P PVGE  + GA G+DG  G +GP
Sbjct: 1412 IPGPVGEQGLPGAAGQDGPPGPMGP 1436

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>COL14_CAEEL (P18834) Cuticle collagen 14|
          Length = 345

 Score = 31.6 bits (70), Expect = 1.9
 Identities = 13/24 (54%), Positives = 16/24 (66%)
 Frame = +2

Query: 269 PLPVGEGDVDGAPGRDGHDGDLGP 340
           P P G+G  DGA G DG  G++GP
Sbjct: 158 PGPAGDGGRDGADGVDGTPGEIGP 181

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>RADA_NANEQ (Q74MX9) DNA repair and recombination protein radA|
          Length = 325

 Score = 31.2 bits (69), Expect = 2.4
 Identities = 15/39 (38%), Positives = 23/39 (58%)
 Frame = -2

Query: 457 LGLGAQISISAEFYGEYNWGQTMEKKVRHEVEYEAAVPP 341
           LG G + +   EFYGEY  G+T   +V H++  +  +PP
Sbjct: 100 LGGGIETAALTEFYGEYGSGKT---QVGHQLAVDVQLPP 135

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>CO5A3_HUMAN (P25940) Collagen alpha-3(V) chain precursor|
          Length = 1745

 Score = 30.8 bits (68), Expect = 3.2
 Identities = 14/25 (56%), Positives = 17/25 (68%)
 Frame = +2

Query: 266  VPLPVGEGDVDGAPGRDGHDGDLGP 340
            +P PVGE  + GAPG+ G  G LGP
Sbjct: 1362 IPGPVGEPGLLGAPGQMGPPGPLGP 1386

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>CO4A4_RABIT (P55787) Collagen alpha-4(IV) chain (Fragment)|
          Length = 623

 Score = 30.8 bits (68), Expect = 3.2
 Identities = 13/24 (54%), Positives = 13/24 (54%)
 Frame = +2

Query: 269 PLPVGEGDVDGAPGRDGHDGDLGP 340
           P P G     G PG DGHDG  GP
Sbjct: 236 PGPPGPPGCQGVPGCDGHDGQKGP 259

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>CO1A2_HUMAN (P08123) Collagen alpha-2(I) chain precursor|
          Length = 1366

 Score = 30.4 bits (67), Expect = 4.2
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +2

Query: 275 PVGEGDVDGAPGRDGHDGDLGP 340
           P GE    G PGRDG DG  GP
Sbjct: 40  PRGERGPPGPPGRDGEDGPTGP 61

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>COL8_CAEEL (P18833) Cuticle collagen 8 precursor|
          Length = 282

 Score = 30.0 bits (66), Expect = 5.4
 Identities = 15/26 (57%), Positives = 15/26 (57%), Gaps = 1/26 (3%)
 Frame = +2

Query: 269 PLPVGEGDVDGAPGRDGHDGDLG-PG 343
           P P GEG   G PG DG DG  G PG
Sbjct: 97  PGPPGEGGQSGEPGHDGDDGKPGAPG 122

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>CO1A2_CHICK (P02467) Collagen alpha-2(I) chain precursor (Fragments)|
          Length = 1362

 Score = 30.0 bits (66), Expect = 5.4
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +2

Query: 275 PVGEGDVDGAPGRDGHDGDLGP 340
           P GE    G PGRDG DG  GP
Sbjct: 41  PQGERGPPGPPGRDGEDGPPGP 62

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>AROK_METTH (O26896) Shikimate kinase (EC 2.7.1.71) (SK)|
          Length = 289

 Score = 30.0 bits (66), Expect = 5.4
 Identities = 24/100 (24%), Positives = 44/100 (44%), Gaps = 5/100 (5%)
 Frame = -2

Query: 502 LKLGYNSTIRAGFPKLGLGAQISISAEFYGEYNWGQTMEKKV--RHEVEYEAA---VPPR 338
           L +  +++++AG    G  A    SA FYG       ME+++  R  +E +     +P R
Sbjct: 122 LNMAVDASLQAGVSVTG--AYDDASASFYGGLTVTDNMERRIILREPMENQKVLIYMPDR 179

Query: 337 TKVTVMAIATRSTVDVPFTYWQRDVTIDGKVEETLMRDGL 218
             +T  +   R  +  P+        +DG+V   L  +G+
Sbjct: 180 KSLTAQSDVPRMKLLAPWVDMAFREVLDGRVHSALTLNGI 219

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>CO3A1_BOVIN (P04258) Collagen alpha-1(III) chain|
          Length = 1049

 Score = 29.6 bits (65), Expect = 7.1
 Identities = 12/20 (60%), Positives = 13/20 (65%)
 Frame = +2

Query: 281 GEGDVDGAPGRDGHDGDLGP 340
           G   VDGAPG+DG  G  GP
Sbjct: 591 GSSGVDGAPGKDGPRGPTGP 610

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>HLYS_HYDAT (Q52SK7) Hydralysin|
          Length = 243

 Score = 29.6 bits (65), Expect = 7.1
 Identities = 22/74 (29%), Positives = 36/74 (48%)
 Frame = -2

Query: 472 AGFPKLGLGAQISISAEFYGEYNWGQTMEKKVRHEVEYEAAVPPRTKVTVMAIATRSTVD 293
           +GF K G    +S++A   G      ++EK    +++    VPPR+KVTV  +       
Sbjct: 126 SGFFKTGAEFSVSVTAGKSGS----SSVEKSSTSQIQ--VTVPPRSKVTVNMVGIMKKEK 179

Query: 292 VPFTYWQRDVTIDG 251
           V F   +  +T+DG
Sbjct: 180 VCF---EVPITVDG 190

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>C1QC_MOUSE (Q02105) Complement C1q subcomponent subunit C precursor|
          Length = 246

 Score = 29.6 bits (65), Expect = 7.1
 Identities = 12/20 (60%), Positives = 14/20 (70%)
 Frame = +2

Query: 281 GEGDVDGAPGRDGHDGDLGP 340
           G   + GAPG+DGHDG  GP
Sbjct: 38  GMPGMPGAPGKDGHDGLQGP 57

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>CO7A1_HUMAN (Q02388) Collagen alpha-1(VII) chain precursor (Long-chain collagen)|
            (LC collagen)
          Length = 2944

 Score = 29.3 bits (64), Expect = 9.3
 Identities = 13/24 (54%), Positives = 15/24 (62%)
 Frame = +2

Query: 269  PLPVGEGDVDGAPGRDGHDGDLGP 340
            P P GE  + G PGR+G  G LGP
Sbjct: 2418 PGPSGERGLAGPPGREGIPGPLGP 2441

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>HLDD_NEIMB (Q9K002) ADP-L-glycero-D-manno-heptose-6-epimerase (EC 5.1.3.20)|
           (ADP-L-glycero-beta-D-manno-heptose-6-epimerase)
           (ADP-glyceromanno-heptose 6-epimerase) (ADP-hep
           6-epimerase) (AGME)
          Length = 334

 Score = 29.3 bits (64), Expect = 9.3
 Identities = 13/35 (37%), Positives = 19/35 (54%)
 Frame = -2

Query: 253 GKVEETLMRDGLYTGINSYNFNFDTVEEKLPERIP 149
           G   +T+  DGLY   N+Y +  D ++    ERIP
Sbjct: 78  GACSDTMNHDGLYMMDNNYQYTLDLLDWCQDERIP 112

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>HLDD_NEIGO (Q51061) ADP-L-glycero-D-manno-heptose-6-epimerase (EC 5.1.3.20)|
           (ADP-L-glycero-beta-D-manno-heptose-6-epimerase)
           (ADP-glyceromanno-heptose 6-epimerase) (ADP-hep
           6-epimerase) (AGME)
          Length = 334

 Score = 29.3 bits (64), Expect = 9.3
 Identities = 13/35 (37%), Positives = 19/35 (54%)
 Frame = -2

Query: 253 GKVEETLMRDGLYTGINSYNFNFDTVEEKLPERIP 149
           G   +T+  DGLY   N+Y +  D ++    ERIP
Sbjct: 78  GACSDTMNHDGLYMMENNYQYTLDLLDWCQDERIP 112

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>COIA1_MOUSE (P39061) Collagen alpha-1(XVIII) chain precursor [Contains:|
           Endostatin]
          Length = 1774

 Score = 29.3 bits (64), Expect = 9.3
 Identities = 13/23 (56%), Positives = 13/23 (56%)
 Frame = +2

Query: 269 PLPVGEGDVDGAPGRDGHDGDLG 337
           P P G    DG PGRDG  GD G
Sbjct: 828 PGPQGPPGKDGTPGRDGEPGDPG 850

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>COL19_CAEEL (P18835) Cuticle collagen 19 precursor|
          Length = 289

 Score = 29.3 bits (64), Expect = 9.3
 Identities = 15/26 (57%), Positives = 15/26 (57%), Gaps = 1/26 (3%)
 Frame = +2

Query: 269 PLPVGEGDVDGAPGRDGHDGDLG-PG 343
           P P GEG   G PG DG DG  G PG
Sbjct: 103 PGPPGEGGQKGNPGHDGDDGKPGAPG 128

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>CLPB_AQUAE (O67588) Chaperone clpB|
          Length = 1006

 Score = 29.3 bits (64), Expect = 9.3
 Identities = 16/92 (17%), Positives = 44/92 (47%)
 Frame = -2

Query: 427 AEFYGEYNWGQTMEKKVRHEVEYEAAVPPRTKVTVMAIATRSTVDVPFTYWQRDVTIDGK 248
           A  YG++   +T+E ++R     E ++  + +    +++T    +    + +  ++IDG 
Sbjct: 125 ARRYGDWWTAETLELELRQLEATEQSIRRQLEQVEKSLSTVFAPEDVKAFLENKLSIDGL 184

Query: 247 VEETLMRDGLYTGINSYNFNFDTVEEKLPERI 152
           + + L +  L   +     + D + +K+ E++
Sbjct: 185 IRKALEKSPLIEQVKELGISPDRIIDKVAEKV 216


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 62,529,846
Number of Sequences: 219361
Number of extensions: 1137352
Number of successful extensions: 5442
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 3756
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5362
length of database: 80,573,946
effective HSP length: 107
effective length of database: 57,102,319
effective search space used: 5367617986
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

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full-length cDNA and EST

Clone Information

BLAST Search Result