ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd21h18
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1NOS3_BOVIN (P29473) Nitric-oxide synthase, endothelial (EC 1.14.... 32 1.2
2RPA4_YEAST (P46669) DNA-dependent RNA polymerase 36 kDa polypept... 31 1.6
3NOS3_RAT (Q62600) Nitric-oxide synthase, endothelial (EC 1.14.13... 31 1.6
4YNG5_YEAST (P53943) Hypothetical 65.3 kDa protein in SUN4-MAS5 i... 31 2.1
5GYRA_MYCLE (Q57532) DNA gyrase subunit A (EC 5.99.1.3) [Contains... 30 2.7
6NOS3_MOUSE (P70313) Nitric-oxide synthase, endothelial (EC 1.14.... 30 2.7
7HCN3_RAT (Q9JKA8) Potassium/sodium hyperpolarization-activated c... 29 7.8
8RAD26_SCHPO (P36632) DNA repair protein rad26 29 7.8

>NOS3_BOVIN (P29473) Nitric-oxide synthase, endothelial (EC 1.14.13.39) (EC-NOS)|
            (NOS type III) (NOSIII) (Endothelial NOS) (eNOS)
            (Constitutive NOS) (cNOS)
          Length = 1204

 Score = 31.6 bits (70), Expect = 1.2
 Identities = 37/129 (28%), Positives = 51/129 (39%), Gaps = 3/129 (2%)
 Frame = +3

Query: 30   LGENCSSAQLCPNLLD*HIRKIVTSITSFRCTYIITARV-SSQSTQG--LISLHTTKKTT 200
            L       QL P L+  H RK+      F+ T +    + SS+ST+   L+ L T  +  
Sbjct: 738  LSTQAEGLQLLPGLIHVHRRKM------FQATVLSVENLQSSKSTRATILVRLDTAGQEG 791

Query: 201  LQSCLRNLSRYRPRNRPGNSEQKLI*LVDHFQXXXXXXXXXXXXXTVTDEAATPELDKCR 380
            LQ    +     P NRPG  E  L  + D                  T+  A  +L+K  
Sbjct: 792  LQYQPGDHIGICPPNRPGLVEALLSRVEDP--------------PPPTESVAVEQLEK-G 836

Query: 381  GPGGDPPDW 407
             PGG PP W
Sbjct: 837  SPGGPPPSW 845



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>RPA4_YEAST (P46669) DNA-dependent RNA polymerase 36 kDa polypeptide (EC|
           2.7.7.6) (A43)
          Length = 326

 Score = 31.2 bits (69), Expect = 1.6
 Identities = 20/57 (35%), Positives = 28/57 (49%), Gaps = 6/57 (10%)
 Frame = +2

Query: 107 NLFPMYLHNNSQSFISKHSRLDLPAYNKKDYTTILSQESLKI------SPEESTRKL 259
           +L PMYL N  Q  + +H    +  YN K    +L  E LKI      S E+++ KL
Sbjct: 48  SLAPMYLENPLQGVMKQHLNPLVMKYNNKVGGVVLGYEGLKILDADPLSKEDTSEKL 104



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>NOS3_RAT (Q62600) Nitric-oxide synthase, endothelial (EC 1.14.13.39) (EC-NOS)|
            (NOS type III) (NOSIII) (Endothelial NOS) (eNOS)
            (Constitutive NOS) (cNOS)
          Length = 1201

 Score = 31.2 bits (69), Expect = 1.6
 Identities = 38/129 (29%), Positives = 51/129 (39%), Gaps = 3/129 (2%)
 Frame = +3

Query: 30   LGENCSSAQLCPNLLD*HIRKIVTSITSFRCTYIITARV-SSQSTQG--LISLHTTKKTT 200
            L     S QL P L   H RK+      F+ T +    + SS+ST+   L+ L T  +  
Sbjct: 735  LSTQAESLQLLPRLTHVHRRKM------FQATILSVENLQSSKSTRATILVRLDTGSQEG 788

Query: 201  LQSCLRNLSRYRPRNRPGNSEQKLI*LVDHFQXXXXXXXXXXXXXTVTDEAATPELDKCR 380
            LQ    +     P NRPG  E  L  + D                  T+  A  +L+K  
Sbjct: 789  LQYQPGDHIGVCPPNRPGLVEALLSRVEDPPPS--------------TEPVAVEQLEK-G 833

Query: 381  GPGGDPPDW 407
             PGG PP W
Sbjct: 834  SPGGPPPGW 842



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>YNG5_YEAST (P53943) Hypothetical 65.3 kDa protein in SUN4-MAS5 intergenic|
           region
          Length = 586

 Score = 30.8 bits (68), Expect = 2.1
 Identities = 16/36 (44%), Positives = 23/36 (63%)
 Frame = +3

Query: 105 ITSFRCTYIITARVSSQSTQGLISLHTTKKTTLQSC 212
           ITSF C+Y   + +S+ ST  L+ L+ TK +T  SC
Sbjct: 465 ITSFVCSYCAMSTLST-STTLLVDLYPTKSSTASSC 499



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>GYRA_MYCLE (Q57532) DNA gyrase subunit A (EC 5.99.1.3) [Contains: Mle gyrA|
           intein]
          Length = 1273

 Score = 30.4 bits (67), Expect = 2.7
 Identities = 17/45 (37%), Positives = 24/45 (53%)
 Frame = +3

Query: 48  SAQLCPNLLD*HIRKIVTSITSFRCTYIITARVSSQSTQGLISLH 182
           SA++  ++ D  IR IVT +T  R  Y   A V+    Q + SLH
Sbjct: 489 SAEIVGHIADPDIRAIVTDLTDGRFYYARVASVTDTGIQPVFSLH 533



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>NOS3_MOUSE (P70313) Nitric-oxide synthase, endothelial (EC 1.14.13.39) (EC-NOS)|
            (NOS type III) (NOSIII) (Endothelial NOS) (eNOS)
            (Constitutive NOS) (cNOS)
          Length = 1201

 Score = 30.4 bits (67), Expect = 2.7
 Identities = 38/129 (29%), Positives = 51/129 (39%), Gaps = 3/129 (2%)
 Frame = +3

Query: 30   LGENCSSAQLCPNLLD*HIRKIVTSITSFRCTYIITARV-SSQSTQG--LISLHTTKKTT 200
            L     S QL P L   H RK+      F+ T +    + SS+ST+   L+ L T  +  
Sbjct: 735  LSTQAESLQLLPGLTHVHRRKM------FQATILSVENLQSSKSTRATILVRLDTGGQEG 788

Query: 201  LQSCLRNLSRYRPRNRPGNSEQKLI*LVDHFQXXXXXXXXXXXXXTVTDEAATPELDKCR 380
            LQ    +     P NRPG  E  L  + D                  T+  A  +L+K  
Sbjct: 789  LQYQPGDHIGVCPPNRPGLVEALLSRVEDPPPS--------------TEPVAVEQLEK-G 833

Query: 381  GPGGDPPDW 407
             PGG PP W
Sbjct: 834  SPGGPPPGW 842



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>HCN3_RAT (Q9JKA8) Potassium/sodium hyperpolarization-activated cyclic|
           nucleotide-gated channel 3
          Length = 780

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 19/51 (37%), Positives = 28/51 (54%), Gaps = 5/51 (9%)
 Frame = -2

Query: 491 PKGARISGSQPA--DRAHGRALDN---SGAKQLPVRGVTSGPPTLVKLRRS 354
           P+G  +S SQP+   RA G        SG+++LP  G+ + PP  V+  RS
Sbjct: 713 PRGRPLSASQPSLPQRATGDGSPRRKGSGSERLPPSGLLAKPPGTVQPSRS 763



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>RAD26_SCHPO (P36632) DNA repair protein rad26|
          Length = 614

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 16/54 (29%), Positives = 26/54 (48%)
 Frame = +3

Query: 27  SLGENCSSAQLCPNLLD*HIRKIVTSITSFRCTYIITARVSSQSTQGLISLHTT 188
           SL E+C   Q  P+L+   +  I++S T   C  +       +S + L+ L TT
Sbjct: 401 SLFESCIRVQNAPSLIKCGVCLIISSTTPSFCASVNLLNADDKSQESLMQLFTT 454


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 71,435,304
Number of Sequences: 219361
Number of extensions: 1386944
Number of successful extensions: 3208
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 3110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3202
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3478785780
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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