ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd20n22
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1SRK6_BRAOE (Q09092) Putative serine/threonine-protein kinase rec... 49 5e-06
2BAK1_ARATH (Q94F62) BRASSINOSTEROID INSENSITIVE 1-associated rec... 36 0.042
3CNGC9_ARATH (Q9M0A4) Putative cyclic nucleotide-gated ion channe... 35 0.072
4RLCK7_ARATH (Q9LQQ8) Probable serine/threonine-protein kinase RL... 34 0.16
5RLK5_ARATH (P47735) Receptor-like protein kinase 5 precursor (EC... 33 0.27
6API_ACHLY (P15636) Protease 1 precursor (EC 3.4.21.50) (Protease... 31 1.0
7KRAF1_DROME (P11346) Raf homolog serine/threonine-protein kinase... 30 1.8
8MED5_YEAST (P53114) RNA polymerase II mediator complex subunit 5... 29 3.9
9MDM36_YEAST (Q06820) Mitochondrial distribution and morphology p... 29 3.9
10FHOD1_MOUSE (Q6P9Q4) FH1/FH2 domain-containing protein (Formin h... 28 6.7
11ZP3R_CAVPO (O08569) Zona pellucida 3 receptor precursor (Acrosom... 28 6.7
12KR15_ICHV1 (Q00097) Gene 15 protein kinase (EC 2.7.11.1) 28 6.7
13RAD54_HUMAN (Q92698) DNA repair and recombination protein RAD54-... 28 8.8

>SRK6_BRAOE (Q09092) Putative serine/threonine-protein kinase receptor|
           precursor (EC 2.7.11.1) (S-receptor kinase) (SRK)
          Length = 849

 Score = 48.9 bits (115), Expect = 5e-06
 Identities = 22/50 (44%), Positives = 31/50 (62%)
 Frame = -1

Query: 375 QVRRCIQVALLCVDMNPRNRPLMSSIVMMLATENATLPEPNEPGAXVGKS 226
           +V +CIQ+ LLCV     +RP MSS+V M  +E   +P+P  PG  V +S
Sbjct: 777 EVLKCIQIGLLCVQELAEHRPAMSSVVWMFGSEATEIPQPKPPGYCVRRS 826



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>BAK1_ARATH (Q94F62) BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1|
           precursor (EC 2.7.11.1) (BRI1-associated receptor kinase
           1) (Somatic embryogenesis receptor-like kinase 3)
          Length = 615

 Score = 35.8 bits (81), Expect = 0.042
 Identities = 18/45 (40%), Positives = 24/45 (53%)
 Frame = -1

Query: 420 ELLXEAMMGSXCDHSQVRRCIQVALLCVDMNPRNRPLMSSIVMML 286
           E L +  +       +V + IQVALLC   +P  RP MS +V ML
Sbjct: 519 EALVDVDLQGNYKDEEVEQLIQVALLCTQSSPMERPKMSEVVRML 563



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>CNGC9_ARATH (Q9M0A4) Putative cyclic nucleotide-gated ion channel 9 (Cyclic|
           nucleotide- and calmodulin-regulated ion channel 9)
          Length = 733

 Score = 35.0 bits (79), Expect = 0.072
 Identities = 25/73 (34%), Positives = 32/73 (43%)
 Frame = +3

Query: 12  WTLICSTMFCICL*K*PINNXVIGNPQLDMYASHTKVCPLPLHLNQSTQTTEYP*QLRST 191
           WT +CS     C      N    GN ++D YA+ T +    L LN    TT+ P      
Sbjct: 308 WTKVCSNSSLDC----HRNFLFCGNEKMDGYAAWTTIKDSVLQLNCPVNTTDNP------ 357

Query: 192 HFEFGIILRPTCS 230
            F+FGI LR   S
Sbjct: 358 PFDFGIYLRALSS 370



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>RLCK7_ARATH (Q9LQQ8) Probable serine/threonine-protein kinase RLCKVII (EC|
           2.7.11.1)
          Length = 423

 Score = 33.9 bits (76), Expect = 0.16
 Identities = 14/49 (28%), Positives = 28/49 (57%)
 Frame = -1

Query: 366 RCIQVALLCVDMNPRNRPLMSSIVMMLATENATLPEPNEPGAXVGKSTS 220
           + + ++ +CV   P  RP++S +V+ L    ++  +PN P +  GK+ S
Sbjct: 351 QALAISAMCVQEQPTMRPVVSDVVLALNFLASSKYDPNSPSSSSGKNPS 399



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>RLK5_ARATH (P47735) Receptor-like protein kinase 5 precursor (EC 2.7.11.1)|
          Length = 999

 Score = 33.1 bits (74), Expect = 0.27
 Identities = 20/64 (31%), Positives = 32/64 (50%), Gaps = 2/64 (3%)
 Frame = -1

Query: 375  QVRRCIQVALLCVDMNPRNRPLMSSIVMMLATENATLP--EPNEPGAXVGKSTSDGELSQ 202
            ++ + I + LLC    P NRP M  +V+ML   +  +P   PN       +S + G+LS 
Sbjct: 935  EISKVIHIGLLCTSPLPLNRPSMRKVVIMLQEVSGAVPCSSPN----TSKRSKTGGKLSP 990

Query: 201  TQSE 190
              +E
Sbjct: 991  YYTE 994



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>API_ACHLY (P15636) Protease 1 precursor (EC 3.4.21.50) (Protease I) (API)|
           (Lysyl endopeptidase)
          Length = 653

 Score = 31.2 bits (69), Expect = 1.0
 Identities = 18/45 (40%), Positives = 25/45 (55%)
 Frame = -1

Query: 306 SSIVMMLATENATLPEPNEPGAXVGKSTSDGELSQTQSELTVTVT 172
           +SIV+    +N+T   PN P +    +  DG +SQTQS  TV  T
Sbjct: 272 ASIVVYWNYQNSTCRAPNTPASG---ANGDGSMSQTQSGSTVKAT 313



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>KRAF1_DROME (P11346) Raf homolog serine/threonine-protein kinase dRAF-1 (EC|
           2.7.11.1) (Protein pole-hole)
          Length = 739

 Score = 30.4 bits (67), Expect = 1.8
 Identities = 22/68 (32%), Positives = 33/68 (48%), Gaps = 5/68 (7%)
 Frame = -1

Query: 384 DHSQVRRCIQVALL-----CVDMNPRNRPLMSSIVMMLATENATLPEPNEPGAXVGKSTS 220
           D SQVR     AL      C+   P++RPL   ++ ML     TLP+       + +S S
Sbjct: 649 DMSQVRSDAPQALKRLAEDCIKYTPKDRPLFRPLLNMLENMLRTLPK-------IHRSAS 701

Query: 219 DGELSQTQ 196
           +  L+Q+Q
Sbjct: 702 EPNLTQSQ 709



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>MED5_YEAST (P53114) RNA polymerase II mediator complex subunit 5 (Negative|
           regulator of URS2 protein 1)
          Length = 1132

 Score = 29.3 bits (64), Expect = 3.9
 Identities = 20/64 (31%), Positives = 29/64 (45%), Gaps = 3/64 (4%)
 Frame = -1

Query: 348 LLCVDMNPRNRPLMSSIVMMLATENATLPEP---NEPGAXVGKSTSDGELSQTQSELTVT 178
           +L +DMN  N+ L++ +  +L T     P P      G   G  T   +LSQT   L   
Sbjct: 169 ILSLDMNSMNKELIAIVSKLLQTTLKLSPSPILLTSAGCKNGSFTLLNQLSQTNKLLFKR 228

Query: 177 VTDT 166
           V+ T
Sbjct: 229 VSQT 232



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>MDM36_YEAST (Q06820) Mitochondrial distribution and morphology protein 36|
          Length = 579

 Score = 29.3 bits (64), Expect = 3.9
 Identities = 16/49 (32%), Positives = 26/49 (53%)
 Frame = -1

Query: 324 RNRPLMSSIVMMLATENATLPEPNEPGAXVGKSTSDGELSQTQSELTVT 178
           RN   MSS V  + ++N+TL  P+ P +    ST+  + S   S L ++
Sbjct: 460 RNEHAMSSSVSSIISQNSTLASPSPPMSSSFISTAPSQSSSRMSTLPLS 508



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>FHOD1_MOUSE (Q6P9Q4) FH1/FH2 domain-containing protein (Formin homolog|
           overexpressed in spleen) (FHOS) (Formin homology 2
           domain-containing protein 1)
          Length = 1196

 Score = 28.5 bits (62), Expect = 6.7
 Identities = 16/55 (29%), Positives = 27/55 (49%)
 Frame = -1

Query: 372 VRRCIQVALLCVDMNPRNRPLMSSIVMMLATENATLPEPNEPGAXVGKSTSDGEL 208
           V+  +++ L+ V+ +  N PL    V  +A+   TLP  N       K+ +D EL
Sbjct: 218 VKTALKLLLVFVEYSENNAPLFIQAVNAVASATGTLPWANLVSILEEKNGADAEL 272



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>ZP3R_CAVPO (O08569) Zona pellucida 3 receptor precursor (Acrosomal matrix|
           component 67) (Protein AM67) (p67)
          Length = 533

 Score = 28.5 bits (62), Expect = 6.7
 Identities = 11/26 (42%), Positives = 15/26 (57%)
 Frame = -2

Query: 317 GHSCLQLS*CWPPRMPRCQSQMSLEP 240
           G S  Q    W P+MP C+S + L+P
Sbjct: 389 GRSSCQADGMWNPKMPACESAVCLKP 414



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>KR15_ICHV1 (Q00097) Gene 15 protein kinase (EC 2.7.11.1)|
          Length = 380

 Score = 28.5 bits (62), Expect = 6.7
 Identities = 15/46 (32%), Positives = 23/46 (50%), Gaps = 3/46 (6%)
 Frame = -1

Query: 387 CDHSQVRRCIQVALLCVDMNPRNRPLMSSIVMMLA---TENATLPE 259
           C+H      + +A  C+  NPR RP ++ ++  L     E AT PE
Sbjct: 334 CEHRAFLDYLTIARGCLRTNPRERPRLTLLIAQLTKFIREVATQPE 379



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>RAD54_HUMAN (Q92698) DNA repair and recombination protein RAD54-like (EC|
           3.6.1.-) (RAD54 homolog) (hRAD54) (hHR54)
          Length = 747

 Score = 28.1 bits (61), Expect = 8.8
 Identities = 23/73 (31%), Positives = 33/73 (45%), Gaps = 2/73 (2%)
 Frame = +1

Query: 25  VARCSVYVYRNNLSTTX*SETHN*ICMLHTQKYALFRSISTSPPK--LPSIRNSYGQLTL 198
           +  C +  +R  LS      T+   C+  +Q  A  RSI + P K  +P+ +   G   L
Sbjct: 43  IQECFLSPFRKPLSQL----TNQPPCLDSSQHEAFIRSILSKPFKVPIPNYQGPLGSRAL 98

Query: 199 SLG*FSVRRALSD 237
            L    VRRAL D
Sbjct: 99  GLKRAGVRRALHD 111


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56,974,623
Number of Sequences: 219361
Number of extensions: 1047119
Number of successful extensions: 2249
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 2190
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2249
length of database: 80,573,946
effective HSP length: 100
effective length of database: 58,637,846
effective search space used: 2286875994
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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