ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd20j04
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1dbj|AK061909.1| Oryza sativa (japonica cultivar-group) cDNA clon... 107 4e-20
2dbj|AP008212.1| Oryza sativa (japonica cultivar-group) genomic D... 107 4e-20
3dbj|AP005446.3| Oryza sativa (japonica cultivar-group) genomic D... 107 4e-20
4dbj|AK066355.1| Oryza sativa (japonica cultivar-group) cDNA clon... 107 4e-20
5ref|XM_464513.1| Oryza sativa (japonica cultivar-group), mRNA 46 0.13
6dbj|AP008208.1| Oryza sativa (japonica cultivar-group) genomic D... 46 0.13
7dbj|AP004869.3| Oryza sativa (japonica cultivar-group) genomic D... 46 0.13
8dbj|AB118005.1| Oryza sativa (japonica cultivar-group) bip118 mR... 46 0.13
9ref|XM_756473.1| Ustilago maydis 521 hypothetical protein (UM054... 44 0.52
10gb|CP000117.1| Anabaena variabilis ATCC 29413 chromosome, comple... 42 2.0
11emb|CR513786.5| Zebrafish DNA sequence from clone CH211-92F19 in... 42 2.0
12gb|AC106886.3| Homo sapiens chromosome 16 clone RP11-2C24, compl... 42 2.0
13gb|AC093249.4| Homo sapiens chromosome 16 clone RP11-146F11, com... 42 2.0
14ref|NM_126110.1| Arabidopsis thaliana 3'-5' exonuclease/ DNA bin... 40 8.1
15gb|AC167129.5| Mus musculus chromosome 5, clone RP23-397D16, com... 40 8.1
16gb|AC166816.2| Mus musculus chromosome 5, clone RP24-321O22, com... 40 8.1
17emb|AL592067.4| Human DNA sequence from clone RP11-361H7 on chro... 40 8.1
18emb|AL355003.10| Human DNA sequence from clone RP11-753M10 on ch... 40 8.1
19emb|AL157700.13| Human DNA sequence from clone RP6-201G10 on chr... 40 8.1
20emb|AL023803.3|HS616B8 Human DNA sequence from clone RP4-616B8 o... 40 8.1
21gb|AC113615.3| Homo sapiens BAC clone RP11-586D19 from 4, comple... 40 8.1
22gb|AC155716.7| Mus musculus 10 BAC RP24-177O15 (Roswell Park Can... 40 8.1
23emb|BX000980.13| Zebrafish DNA sequence from clone CH211-136D9, ... 40 8.1
24gb|AC108046.3| Homo sapiens BAC clone RP11-308K2 from 4, complet... 40 8.1
25ref|NM_001034950.1| Rattus norvegicus urinary protein 2 (Rup2), ... 40 8.1
26gb|AC012673.37| Homo sapiens 12 BAC RP11-431G15 (Roswell Park Ca... 40 8.1
27gb|AF368860.1|AF368860 Rattus norvegicus 3' non-translated beta-... 40 8.1
28gb|AC015651.18|AC015651 Homo sapiens chromosome 17, clone RP11-5... 40 8.1
29dbj|AB020742.1| Arabidopsis thaliana genomic DNA, chromosome 5, ... 40 8.1
30gb|AC115835.6| Mus musculus chromosome 3, clone RP24-184C18, com... 40 8.1
31emb|AL773557.21| Mouse DNA sequence from clone RP23-391F9 on chr... 40 8.1
32emb|AL935126.18| Zebrafish DNA sequence from clone CH211-186J3, ... 40 8.1
33gb|AC159462.6| Mus musculus BAC clone RP23-177D16 from chromosom... 40 8.1

>dbj|AK061909.1| Oryza sativa (japonica cultivar-group) cDNA clone:001-042-A02, full
           insert sequence
          Length = 1392

 Score =  107 bits (54), Expect = 4e-20
 Identities = 108/126 (85%)
 Strand = Plus / Minus

                                                                       
Query: 468 tgggaggccctttggtcgaggctcagacgaactggacggcttgaaggattggtacttact 527
           |||||||||||||  || |||||||||||| |||| ||||||||||||  ||||||| ||
Sbjct: 858 tgggaggccctttcatccaggctcagacgagctgggcggcttgaaggaacggtactttct 799

                                                                       
Query: 528 gttgtgactgctgaggttgtgttctttgagcgaccaagctttggtgatcgaggtctggtg 587
              |||  | ||||| |||||||||||||||| |||||||||||||||| || |||||||
Sbjct: 798 tcagtgcttactgagcttgtgttctttgagcggccaagctttggtgatctagctctggtg 739

                 
Query: 588 gtaggg 593
           ||||||
Sbjct: 738 gtaggg 733



 Score = 61.9 bits (31), Expect = 2e-06
 Identities = 43/47 (91%)
 Strand = Plus / Minus

                                                          
Query: 391 ctgtctgctctgatggcaatttggggagagatttccgttgggacttc 437
           |||| ||||||||||||| |||||||||||| |||||||||| ||||
Sbjct: 944 ctgtttgctctgatggcagtttggggagagacttccgttggggcttc 898



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>dbj|AP008212.1| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 6, complete
                sequence
          Length = 30731886

 Score =  107 bits (54), Expect = 4e-20
 Identities = 108/126 (85%)
 Strand = Plus / Plus

                                                                            
Query: 468      tgggaggccctttggtcgaggctcagacgaactggacggcttgaaggattggtacttact 527
                |||||||||||||  || |||||||||||| |||| ||||||||||||  ||||||| ||
Sbjct: 23708168 tgggaggccctttcatccaggctcagacgagctgggcggcttgaaggaacggtactttct 23708227

                                                                            
Query: 528      gttgtgactgctgaggttgtgttctttgagcgaccaagctttggtgatcgaggtctggtg 587
                   |||  | ||||| |||||||||||||||| |||||||||||||||| || |||||||
Sbjct: 23708228 tcagtgcttactgagcttgtgttctttgagcggccaagctttggtgatctagctctggtg 23708287

                      
Query: 588      gtaggg 593
                ||||||
Sbjct: 23708288 gtaggg 23708293



 Score = 61.9 bits (31), Expect = 2e-06
 Identities = 43/47 (91%)
 Strand = Plus / Plus

                                                               
Query: 391      ctgtctgctctgatggcaatttggggagagatttccgttgggacttc 437
                |||| ||||||||||||| |||||||||||| |||||||||| ||||
Sbjct: 23708082 ctgtttgctctgatggcagtttggggagagacttccgttggggcttc 23708128



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>dbj|AP005446.3| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 6, PAC
             clone:P0029C06
          Length = 156500

 Score =  107 bits (54), Expect = 4e-20
 Identities = 108/126 (85%)
 Strand = Plus / Plus

                                                                         
Query: 468   tgggaggccctttggtcgaggctcagacgaactggacggcttgaaggattggtacttact 527
             |||||||||||||  || |||||||||||| |||| ||||||||||||  ||||||| ||
Sbjct: 56244 tgggaggccctttcatccaggctcagacgagctgggcggcttgaaggaacggtactttct 56303

                                                                         
Query: 528   gttgtgactgctgaggttgtgttctttgagcgaccaagctttggtgatcgaggtctggtg 587
                |||  | ||||| |||||||||||||||| |||||||||||||||| || |||||||
Sbjct: 56304 tcagtgcttactgagcttgtgttctttgagcggccaagctttggtgatctagctctggtg 56363

                   
Query: 588   gtaggg 593
             ||||||
Sbjct: 56364 gtaggg 56369



 Score = 61.9 bits (31), Expect = 2e-06
 Identities = 43/47 (91%)
 Strand = Plus / Plus

                                                            
Query: 391   ctgtctgctctgatggcaatttggggagagatttccgttgggacttc 437
             |||| ||||||||||||| |||||||||||| |||||||||| ||||
Sbjct: 56158 ctgtttgctctgatggcagtttggggagagacttccgttggggcttc 56204



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>dbj|AK066355.1| Oryza sativa (japonica cultivar-group) cDNA clone:J013067N05, full
            insert sequence
          Length = 1772

 Score =  107 bits (54), Expect = 4e-20
 Identities = 108/126 (85%)
 Strand = Plus / Minus

                                                                        
Query: 468  tgggaggccctttggtcgaggctcagacgaactggacggcttgaaggattggtacttact 527
            |||||||||||||  || |||||||||||| |||| ||||||||||||  ||||||| ||
Sbjct: 1234 tgggaggccctttcatccaggctcagacgagctgggcggcttgaaggaacggtactttct 1175

                                                                        
Query: 528  gttgtgactgctgaggttgtgttctttgagcgaccaagctttggtgatcgaggtctggtg 587
               |||  | ||||| |||||||||||||||| |||||||||||||||| || |||||||
Sbjct: 1174 tcagtgcttactgagcttgtgttctttgagcggccaagctttggtgatctagctctggtg 1115

                  
Query: 588  gtaggg 593
            ||||||
Sbjct: 1114 gtaggg 1109



 Score = 61.9 bits (31), Expect = 2e-06
 Identities = 43/47 (91%)
 Strand = Plus / Minus

                                                           
Query: 391  ctgtctgctctgatggcaatttggggagagatttccgttgggacttc 437
            |||| ||||||||||||| |||||||||||| |||||||||| ||||
Sbjct: 1320 ctgtttgctctgatggcagtttggggagagacttccgttggggcttc 1274



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>ref|XM_464513.1| Oryza sativa (japonica cultivar-group),  mRNA
          Length = 1188

 Score = 46.1 bits (23), Expect = 0.13
 Identities = 41/47 (87%)
 Strand = Plus / Minus

                                                          
Query: 547 tgttctttgagcgaccaagctttggtgatcgaggtctggtggtaggg 593
           ||||||| |||||||| ||||||||||||| || ||| |||| ||||
Sbjct: 838 tgttcttggagcgaccgagctttggtgatctagctctcgtgggaggg 792



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>dbj|AP008208.1| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 2, complete
               sequence
          Length = 35954743

 Score = 46.1 bits (23), Expect = 0.13
 Identities = 41/47 (87%)
 Strand = Plus / Minus

                                                              
Query: 547     tgttctttgagcgaccaagctttggtgatcgaggtctggtggtaggg 593
               ||||||| |||||||| ||||||||||||| || ||| |||| ||||
Sbjct: 5626663 tgttcttggagcgaccgagctttggtgatctagctctcgtgggaggg 5626617



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>dbj|AP004869.3| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 2, PAC
             clone:P0419A09
          Length = 168064

 Score = 46.1 bits (23), Expect = 0.13
 Identities = 41/47 (87%)
 Strand = Plus / Minus

                                                            
Query: 547   tgttctttgagcgaccaagctttggtgatcgaggtctggtggtaggg 593
             ||||||| |||||||| ||||||||||||| || ||| |||| ||||
Sbjct: 54399 tgttcttggagcgaccgagctttggtgatctagctctcgtgggaggg 54353



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>dbj|AB118005.1| Oryza sativa (japonica cultivar-group) bip118 mRNA for BRI1-KD
           interacting protein 118, partial cds
          Length = 1333

 Score = 46.1 bits (23), Expect = 0.13
 Identities = 41/47 (87%)
 Strand = Plus / Minus

                                                          
Query: 547 tgttctttgagcgaccaagctttggtgatcgaggtctggtggtaggg 593
           ||||||| |||||||| ||||||||||||| || ||| |||| ||||
Sbjct: 656 tgttcttggagcgaccgagctttggtgatctagctctcgtgggaggg 610



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>ref|XM_756473.1| Ustilago maydis 521 hypothetical protein (UM05419.1) partial mRNA
          Length = 1314

 Score = 44.1 bits (22), Expect = 0.52
 Identities = 25/26 (96%)
 Strand = Plus / Minus

                                      
Query: 434  cttcttgggagcaggtgttgtcttgg 459
            |||||||||||||||||||| |||||
Sbjct: 1227 cttcttgggagcaggtgttgccttgg 1202



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>gb|CP000117.1| Anabaena variabilis ATCC 29413 chromosome, complete sequence
          Length = 6365727

 Score = 42.1 bits (21), Expect = 2.0
 Identities = 24/25 (96%)
 Strand = Plus / Minus

                                        
Query: 447     ggtgttgtcttggcgctgttttggg 471
               |||||||||||||||||| ||||||
Sbjct: 3291310 ggtgttgtcttggcgctgatttggg 3291286



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>emb|CR513786.5| Zebrafish DNA sequence from clone CH211-92F19 in linkage group 14,
             complete sequence
          Length = 97391

 Score = 42.1 bits (21), Expect = 2.0
 Identities = 21/21 (100%)
 Strand = Plus / Minus

                                  
Query: 534   actgctgaggttgtgttcttt 554
             |||||||||||||||||||||
Sbjct: 53505 actgctgaggttgtgttcttt 53485



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>gb|AC106886.3| Homo sapiens chromosome 16 clone RP11-2C24, complete sequence
          Length = 207950

 Score = 42.1 bits (21), Expect = 2.0
 Identities = 24/25 (96%)
 Strand = Plus / Minus

                                      
Query: 214   ggctgaggcatgactctcagctttc 238
             |||||||||||||| ||||||||||
Sbjct: 42383 ggctgaggcatgacactcagctttc 42359



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>gb|AC093249.4| Homo sapiens chromosome 16 clone RP11-146F11, complete sequence
          Length = 185664

 Score = 42.1 bits (21), Expect = 2.0
 Identities = 24/25 (96%)
 Strand = Plus / Plus

                                      
Query: 214   ggctgaggcatgactctcagctttc 238
             |||||||||||||| ||||||||||
Sbjct: 43643 ggctgaggcatgacactcagctttc 43667



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>ref|NM_126110.1| Arabidopsis thaliana 3'-5' exonuclease/ DNA binding / DNA-directed
           DNA polymerase/ nucleotide binding AT5G67100 mRNA,
           complete cds
          Length = 4479

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                               
Query: 282 tcatctggagtaacctgagc 301
           ||||||||||||||||||||
Sbjct: 563 tcatctggagtaacctgagc 544



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>gb|AC167129.5| Mus musculus chromosome 5, clone RP23-397D16, complete sequence
          Length = 197551

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                  
Query: 405    ggcaatttggggagagattt 424
              ||||||||||||||||||||
Sbjct: 175477 ggcaatttggggagagattt 175458



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>gb|AC166816.2| Mus musculus chromosome 5, clone RP24-321O22, complete sequence
          Length = 156357

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                  
Query: 405    ggcaatttggggagagattt 424
              ||||||||||||||||||||
Sbjct: 141601 ggcaatttggggagagattt 141620



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>emb|AL592067.4| Human DNA sequence from clone RP11-361H7 on chromosome 13, complete
           sequence
          Length = 83141

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                               
Query: 39  gttactagctacaaataata 58
           ||||||||||||||||||||
Sbjct: 497 gttactagctacaaataata 478



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>emb|AL355003.10| Human DNA sequence from clone RP11-753M10 on chromosome 13, complete
              sequence
          Length = 133309

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                  
Query: 39     gttactagctacaaataata 58
              ||||||||||||||||||||
Sbjct: 131806 gttactagctacaaataata 131787



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>emb|AL157700.13| Human DNA sequence from clone RP6-201G10 on chromosome Xq12-13.1 Contains
              the 3' end of the OPHN1 gene for oligophrenin 1, complete
              sequence
          Length = 190611

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                  
Query: 538    ctgaggttgtgttctttgag 557
              ||||||||||||||||||||
Sbjct: 133616 ctgaggttgtgttctttgag 133597



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>emb|AL023803.3|HS616B8 Human DNA sequence from clone RP4-616B8 on chromosome 20q11.22-12
              Contains the DDX35 gene for DEAD/H box polypeptide 35, the
              NPM1P19 gene for nucleophosmin 1 (nucleolar phosphoprotein
              B23, numatrin) pseudogene 19, the C20orf129 gene for
              chromosome 20 open reading frame 129, the 3' end of the
              PPP1R16B gene for protein phosphatase 1 regulatory
              (inhibitory) subunit 16B and four CpG islands, complete
              sequence
          Length = 155379

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                  
Query: 193    aacaaaaatgcagaaatggg 212
              ||||||||||||||||||||
Sbjct: 129245 aacaaaaatgcagaaatggg 129226



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>gb|AC113615.3| Homo sapiens BAC clone RP11-586D19 from 4, complete sequence
          Length = 164043

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                
Query: 407  caatttggggagagatttcc 426
            ||||||||||||||||||||
Sbjct: 9227 caatttggggagagatttcc 9208



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>gb|AC155716.7| Mus musculus 10 BAC RP24-177O15 (Roswell Park Cancer Institute
             (C57BL/6J Male) Mouse BAC Library) complete sequence
          Length = 154016

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 23/24 (95%)
 Strand = Plus / Plus

                                     
Query: 207   aatgggaggctgaggcatgactct 230
             ||||||||| ||||||||||||||
Sbjct: 71275 aatgggagggtgaggcatgactct 71298



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>emb|BX000980.13| Zebrafish DNA sequence from clone CH211-136D9, complete sequence
          Length = 169191

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                
Query: 359  agaagatatagaagttgcaa 378
            ||||||||||||||||||||
Sbjct: 4482 agaagatatagaagttgcaa 4463



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>gb|AC108046.3| Homo sapiens BAC clone RP11-308K2 from 4, complete sequence
          Length = 177483

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                 
Query: 186   cccaaataacaaaaatgcag 205
             ||||||||||||||||||||
Sbjct: 34193 cccaaataacaaaaatgcag 34212



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>ref|NM_001034950.1| Rattus norvegicus urinary protein 2 (Rup2), mRNA
          Length = 1197

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                               
Query: 201 tgcagaaatgggaggctgag 220
           ||||||||||||||||||||
Sbjct: 917 tgcagaaatgggaggctgag 898



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>gb|AC012673.37| Homo sapiens 12 BAC RP11-431G15 (Roswell Park Cancer Institute Human
             BAC Library) complete sequence
          Length = 47302

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                 
Query: 79    aacacaacacaactgagaag 98
             ||||||||||||||||||||
Sbjct: 20922 aacacaacacaactgagaag 20941



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>gb|AF368860.1|AF368860 Rattus norvegicus 3' non-translated beta-F1-ATPase mRNA-binding
           protein mRNA, complete cds
          Length = 1197

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                               
Query: 201 tgcagaaatgggaggctgag 220
           ||||||||||||||||||||
Sbjct: 917 tgcagaaatgggaggctgag 898



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>gb|AC015651.18|AC015651 Homo sapiens chromosome 17, clone RP11-55A13, complete sequence
          Length = 191583

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                  
Query: 209    tgggaggctgaggcatgact 228
              ||||||||||||||||||||
Sbjct: 117163 tgggaggctgaggcatgact 117144



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>dbj|AB020742.1| Arabidopsis thaliana genomic DNA, chromosome 5, TAC clone:K21H1
          Length = 74342

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 282   tcatctggagtaacctgagc 301
             ||||||||||||||||||||
Sbjct: 19052 tcatctggagtaacctgagc 19033



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>gb|AC115835.6| Mus musculus chromosome 3, clone RP24-184C18, complete sequence
          Length = 172025

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                 
Query: 124   atgttccaaactccaggtga 143
             ||||||||||||||||||||
Sbjct: 14604 atgttccaaactccaggtga 14623



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>emb|AL773557.21| Mouse DNA sequence from clone RP23-391F9 on chromosome 2, complete
             sequence
          Length = 215139

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 204   agaaatgggaggctgaggca 223
             ||||||||||||||||||||
Sbjct: 94009 agaaatgggaggctgaggca 93990



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>emb|AL935126.18| Zebrafish DNA sequence from clone CH211-186J3, complete sequence
          Length = 174986

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                  
Query: 196    aaaaatgcagaaatgggagg 215
              ||||||||||||||||||||
Sbjct: 130409 aaaaatgcagaaatgggagg 130428



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>gb|AC159462.6| Mus musculus BAC clone RP23-177D16 from chromosome 10, complete
             sequence
          Length = 200663

 Score = 40.1 bits (20), Expect = 8.1
 Identities = 23/24 (95%)
 Strand = Plus / Plus

                                     
Query: 207   aatgggaggctgaggcatgactct 230
             ||||||||| ||||||||||||||
Sbjct: 97488 aatgggagggtgaggcatgactct 97511


  Database: nt
    Posted date:  May 29, 2006 11:10 AM
  Number of letters in database: 3,984,495,279
  Number of sequences in database:  917,343
  
  Database: /shigen/export/home/twatanab/db/nt/nt.01
    Posted date:  May 29, 2006 11:16 AM
  Number of letters in database: 3,988,174,986
  Number of sequences in database:  835,257
  
  Database: /shigen/export/home/twatanab/db/nt/nt.02
    Posted date:  May 29, 2006 11:21 AM
  Number of letters in database: 3,991,246,324
  Number of sequences in database:  771,481
  
  Database: /shigen/export/home/twatanab/db/nt/nt.03
    Posted date:  May 29, 2006 11:27 AM
  Number of letters in database: 3,990,718,311
  Number of sequences in database:  977,174
  
  Database: /shigen/export/home/twatanab/db/nt/nt.04
    Posted date:  May 29, 2006 11:29 AM
  Number of letters in database: 1,278,410,368
  Number of sequences in database:  400,813
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 7,197,700
Number of Sequences: 3902068
Number of extensions: 7197700
Number of successful extensions: 707633
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 33
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 707103
Number of HSP's gapped (non-prelim): 526
length of query: 593
length of database: 17,233,045,268
effective HSP length: 23
effective length of query: 570
effective length of database: 17,143,297,704
effective search space: 9771679691280
effective search space used: 9771679691280
T: 0
A: 0
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)
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