ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd20h02
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1GAT1_YEAST (P43574) Transcriptional regulatory protein GAT1 33 0.66
2USH2A_RAT (Q8K3K1) Usherin precursor (Usher syndrome type-2A pro... 30 4.3
3ZFN2B_HUMAN (Q8WV99) AN1-type zinc finger protein 2B 30 5.6
4RR2_SPIOL (P08242) Chloroplast 30S ribosomal protein S2 30 5.6
5RR2_MARPO (P06354) Chloroplast 30S ribosomal protein S2 30 5.6
6BRC4_DROME (Q24206) Broad-complex core-protein isoform 6 30 7.3
7MUC1_YEAST (P08640) Mucin-like protein 1 precursor 30 7.3
8POLG_STEVM (P09732) Genome polyprotein [Contains: Capsid protein... 29 9.6
9KR105_HUMAN (P60370) Keratin-associated protein 10-5 (Keratin-as... 29 9.6
10GYRB_MYXXA (O33367) DNA gyrase subunit B (EC 5.99.1.3) 29 9.6

>GAT1_YEAST (P43574) Transcriptional regulatory protein GAT1|
          Length = 510

 Score = 33.1 bits (74), Expect = 0.66
 Identities = 23/90 (25%), Positives = 41/90 (45%), Gaps = 4/90 (4%)
 Frame = +2

Query: 44  NTIHGGWRRERS*LLRFGCIPHLRRPGSYHTGV----RPCLSAAFHTTSIRSTSSAPTSW 211
           NT         S LLR    P + +PGS         +P L     +TS++S+++ P++ 
Sbjct: 243 NTNTSASTNTNSPLLRRNPSPSIVKPGSRRNSSVRKKKPALKKIKSSTSVQSSATPPSNT 302

Query: 212 CCQPSRKCTTPPAWHACTTPRSIVYSQEPR 301
              P  KC+       CTT  + ++ ++P+
Sbjct: 303 SSNPDIKCSN------CTTSTTPLWRKDPK 326



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>USH2A_RAT (Q8K3K1) Usherin precursor (Usher syndrome type-2A protein homolog)|
            (Usher syndrome type IIa protein homolog)
          Length = 1512

 Score = 30.4 bits (67), Expect = 4.3
 Identities = 21/67 (31%), Positives = 27/67 (40%), Gaps = 12/67 (17%)
 Frame = +2

Query: 98   CIPHLR-------RPGSYH-----TGVRPCLSAAFHTTSIRSTSSAPTSWCCQPSRKCTT 241
            C+PH +       +PG Y      TG  PCL     T +    S     +C  PS   TT
Sbjct: 918  CLPHRQERRCVQCQPGCYSSPSNATGCLPCLCHTVATKNCICNSVTGHCYCPDPS---TT 974

Query: 242  PPAWHAC 262
              +WH C
Sbjct: 975  GLSWHQC 981



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>ZFN2B_HUMAN (Q8WV99) AN1-type zinc finger protein 2B|
          Length = 257

 Score = 30.0 bits (66), Expect = 5.6
 Identities = 22/65 (33%), Positives = 28/65 (43%), Gaps = 11/65 (16%)
 Frame = +2

Query: 98  CIPHLRRP------GSYHTGVRPCLSAAFHTTSIRSTSSAPTSW-----CCQPSRKCTTP 244
           CI H R P      G  H   R  L+A     ++ STS+ P+       C  PSR  T  
Sbjct: 123 CIKH-RHPLDHDCSGEGHPTSRAGLAAISRAQAVASTSTVPSPSQTMPSCTSPSRATTRS 181

Query: 245 PAWHA 259
           P+W A
Sbjct: 182 PSWTA 186



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>RR2_SPIOL (P08242) Chloroplast 30S ribosomal protein S2|
          Length = 236

 Score = 30.0 bits (66), Expect = 5.6
 Identities = 31/104 (29%), Positives = 48/104 (46%), Gaps = 12/104 (11%)
 Frame = -2

Query: 588 KRIRVIMLTMSSERFLK----IQREAPAEFQQYLVQVTKYQAAQNCKT--------WLVG 445
           K I +I LT ++ RFL     +  +A +  +Q+L+  TK +AA +           ++  
Sbjct: 40  KGIHIINLTRTA-RFLSEACDLVFDASSRGKQFLIVGTKNKAADSVARAAIRARCHYVNK 98

Query: 444 KWLSPREQRWAPPGTHFHQFVVPPIIGFRRDCTYGKLAAMRLPK 313
           KWL      W+   T  H+F        R + T G+LA  RLPK
Sbjct: 99  KWLGGMLTNWSTTETRLHKF-----RDLRMEQTAGRLA--RLPK 135



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>RR2_MARPO (P06354) Chloroplast 30S ribosomal protein S2|
          Length = 235

 Score = 30.0 bits (66), Expect = 5.6
 Identities = 24/81 (29%), Positives = 39/81 (48%), Gaps = 12/81 (14%)
 Frame = -2

Query: 591 RKRIRVIMLTMSSERFLK----IQREAPAEFQQYLVQVTKYQAAQNCKT--------WLV 448
           RK I +I LT ++ RFL     +   A ++ +Q+L+  TKYQAA   ++        ++ 
Sbjct: 39  RKGIHIINLTQTA-RFLSEACDLVANASSKGKQFLIVGTKYQAADLIESSALKARCHYVN 97

Query: 447 GKWLSPREQRWAPPGTHFHQF 385
            KWL      W+   T   +F
Sbjct: 98  QKWLGGMLTNWSTIETRLQKF 118



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>BRC4_DROME (Q24206) Broad-complex core-protein isoform 6|
          Length = 880

 Score = 29.6 bits (65), Expect = 7.3
 Identities = 26/88 (29%), Positives = 31/88 (35%), Gaps = 20/88 (22%)
 Frame = +1

Query: 121 RELSHRRQAVLERRLPHDVD------------PVDVERAH-----LVVLPT---LQEMHH 240
           RE    R+   ER L H+ D            P     +H     L +LP+   L   HH
Sbjct: 555 RERERERERERERSLDHERDLERPGGTGSPPPPPPSHHSHFGQHPLSLLPSHHQLHATHH 614

Query: 241 SAGVARVHHPSLHRVLAGAEALHVLGEP 324
               A  HH   H   A A AL   G P
Sbjct: 615 ELSAAAAHHAHAHAHAAHAHALARAGSP 642



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>MUC1_YEAST (P08640) Mucin-like protein 1 precursor|
          Length = 1367

 Score = 29.6 bits (65), Expect = 7.3
 Identities = 18/56 (32%), Positives = 25/56 (44%), Gaps = 4/56 (7%)
 Frame = +2

Query: 155 SAAFHTTSIRSTSSAPTSWCCQPSRKCT----TPPAWHACTTPRSIVYSQEPRPCT 310
           S    +TS  STSS+ T+     +  CT    TPP   +CT  +      +  PCT
Sbjct: 241 STTTSSTSESSTSSSTTAPATPTTTSCTKEKPTPPTTTSCTKEKPTPPHHDTTPCT 296



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>POLG_STEVM (P09732) Genome polyprotein [Contains: Capsid protein C (Core|
            protein); Envelope protein M (Matrix protein); Major
            envelope protein E; Nonstructural protein 1 (NS1);
            Nonstructural protein 2A (NS2A); Flavivirin protease NS2B
            regulatory subunit;
          Length = 1525

 Score = 29.3 bits (64), Expect = 9.6
 Identities = 18/62 (29%), Positives = 25/62 (40%)
 Frame = +2

Query: 119  PGSYHTGVRPCLSAAFHTTSIRSTSSAPTSWCCQPSRKCTTPPAWHACTTPRSIVYSQEP 298
            PG+  T    C +      +  ++    T WCC   R C+ PP  +  TT     Y  E 
Sbjct: 1070 PGTTVTVTEHCGNRGASLRTTTASGKLVTDWCC---RSCSLPPLRY--TTKDGCWYGMEI 1124

Query: 299  RP 304
            RP
Sbjct: 1125 RP 1126



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>KR105_HUMAN (P60370) Keratin-associated protein 10-5 (Keratin-associated|
           protein 10.5) (High sulfur keratin-associated protein
           10.5) (Keratin-associated protein 18-5)
           (Keratin-associated protein 18.5)
          Length = 271

 Score = 29.3 bits (64), Expect = 9.6
 Identities = 16/43 (37%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
 Frame = +2

Query: 146 PCLSAAFHTTSIRS-TSSAPTSWCCQPSRKCTTPPAWHACTTP 271
           PC  AA   +  +S  +S+ T  CCQP+  C + P   AC  P
Sbjct: 50  PCCQAACEPSPCQSGCTSSCTPSCCQPAC-CASSPCQQACCVP 91



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>GYRB_MYXXA (O33367) DNA gyrase subunit B (EC 5.99.1.3)|
          Length = 815

 Score = 29.3 bits (64), Expect = 9.6
 Identities = 14/37 (37%), Positives = 21/37 (56%), Gaps = 6/37 (16%)
 Frame = +2

Query: 158 AAFHTTSIRSTSSAPTSWCCQPS------RKCTTPPA 250
           AA  T+  R+ S+ P SWC  P+      R+C+T P+
Sbjct: 656 AACATSWCRTPSTTPRSWCSTPTRMEPCGRRCSTTPS 692


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 78,611,884
Number of Sequences: 219361
Number of extensions: 1540004
Number of successful extensions: 5119
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4899
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5113
length of database: 80,573,946
effective HSP length: 107
effective length of database: 57,102,319
effective search space used: 5538924943
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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