ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd20c21
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1GSTU6_ORYSA (Q06398) Probable glutathione S-transferase GSTU6 (E... 63 2e-21
2GSTX2_MAIZE (P50472) Probable glutathione S-transferase BZ2 (EC ... 58 3e-08
3GSTX6_SOYBN (P32110) Probable glutathione S-transferase (EC 2.5.... 33 8e-05
4LGUL_SOYBN (P46417) Lactoylglutathione lyase (EC 4.4.1.5) (Methy... 38 8e-05
5GSTX4_TOBAC (Q03666) Probable glutathione S-transferase (EC 2.5.... 34 0.001
6GSTXC_TOBAC (P49332) Probable glutathione S-transferase parC (EC... 35 0.23
7DAG1_BOVIN (O18738) Dystroglycan precursor (Dystrophin-associate... 33 0.87
8DAG1_HUMAN (Q14118) Dystroglycan precursor (Dystrophin-associate... 32 1.5
9DAG1_MOUSE (Q62165) Dystroglycan precursor (Dystrophin-associate... 31 2.5
10YL_DROME (P98163) Putative vitellogenin receptor precursor (Prot... 31 3.3
11GSTXA_TOBAC (P25317) Probable glutathione S-transferase parA (EC... 31 3.3
12DAG1_RABIT (Q28685) Dystroglycan precursor (Dystrophin-associate... 30 4.3
13CTPV_MYCTU (P77894) Probable cation-transporting ATPase V (EC 3.... 30 4.3
14RIMS1_HUMAN (Q86UR5) Regulating synaptic membrane exocytosis pro... 30 4.3
15RGF3_SCHPO (Q9Y7U5) Rho1 guanine nucleotide exchange factor 3 30 7.3
16GSTX1_NICPL (P50471) Probable glutathione S-transferase MSR-1 (E... 30 7.3
17FUT4_PANTR (Q659K9) Alpha-(1,3)-fucosyltransferase (EC 2.4.1.-) ... 29 9.6
18V70K_OYMV (P20130) 66 kDa protein 29 9.6
19UBE13_WHEAT (P31252) Ubiquitin-activating enzyme E1 3 29 9.6
20RIMS1_RAT (Q9JIR4) Regulating synaptic membrane exocytosis prote... 29 9.6
21PZRN4_HUMAN (Q6ZMN7) PDZ domain-containing RING finger protein 4... 29 9.6
22NODAL_HUMAN (Q96S42) Nodal homolog precursor 29 9.6

>GSTU6_ORYSA (Q06398) Probable glutathione S-transferase GSTU6 (EC 2.5.1.18) (28|
           kDa cold-induced protein)
          Length = 236

 Score = 62.8 bits (151), Expect(2) = 2e-21
 Identities = 29/61 (47%), Positives = 39/61 (63%), Gaps = 1/61 (1%)
 Frame = -1

Query: 441 GYIDVVLGGMVAWMQGTEALCGLELLDATKTPLLLAWMERFGGMEPAKAVLP-DIDRLVE 265
           G++DVVLGG + W    + L G  L+D  +TP L AW ERF   + AK V+P D D+L+E
Sbjct: 163 GFVDVVLGGYLGWFTAIDKLIGRRLIDPARTPALAAWEERFRATDAAKGVVPDDADKLLE 222

Query: 264 F 262
           F
Sbjct: 223 F 223



 Score = 58.9 bits (141), Expect(2) = 2e-21
 Identities = 29/59 (49%), Positives = 37/59 (62%), Gaps = 1/59 (1%)
 Frame = -3

Query: 613 AAYIDQNLVAPWWKMFVG-KTDKEKHEGTKQTLAAVEKLEVALRECSSGKPFFGGDNVG 440
           AAY+D  +   W  +  G KT++E+     Q +AA+E LE A  ECS GKPFFGGD VG
Sbjct: 105 AAYVDDKVRPAWLAILFGSKTEEERAAAVAQAVAALETLEGAFGECSKGKPFFGGDGVG 163



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>GSTX2_MAIZE (P50472) Probable glutathione S-transferase BZ2 (EC 2.5.1.18)|
           (Protein bronze-2)
          Length = 236

 Score = 57.8 bits (138), Expect = 3e-08
 Identities = 27/71 (38%), Positives = 42/71 (59%)
 Frame = -1

Query: 453 ETTSGYIDVVLGGMVAWMQGTEALCGLELLDATKTPLLLAWMERFGGMEPAKAVLPDIDR 274
           +   G +D+ LG  +  ++  E L GL L+DA+ TPLL  W +RF     AK VLPD ++
Sbjct: 158 DAAPGLLDLALGCFLPALRACERLHGLSLIDASATPLLDGWSQRFAAHPAAKRVLPDTEK 217

Query: 273 LVEFAKMKRAQ 241
           +V+F +  + Q
Sbjct: 218 VVQFTRFLQVQ 228



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>GSTX6_SOYBN (P32110) Probable glutathione S-transferase (EC 2.5.1.18) (Heat|
           shock protein 26A) (G2-4)
          Length = 225

 Score = 33.5 bits (75), Expect(2) = 8e-05
 Identities = 17/64 (26%), Positives = 29/64 (45%)
 Frame = -1

Query: 441 GYIDVVLGGMVAWMQGTEALCGLELLDATKTPLLLAWMERFGGMEPAKAVLPDIDRLVEF 262
           G +D+    +  W+   + + GL+L  + K P+L  W + F        VLP  D L  +
Sbjct: 154 GLVDIAAVFIAFWIPIFQEIAGLQLFTSEKFPILYKWSQEFLNHPFVHEVLPPRDPLFAY 213

Query: 261 AKMK 250
            K +
Sbjct: 214 FKAR 217



 Score = 32.0 bits (71), Expect(2) = 8e-05
 Identities = 18/57 (31%), Positives = 30/57 (52%)
 Frame = -3

Query: 607 YIDQNLVAPWWKMFVGKTDKEKHEGTKQTLAAVEKLEVALRECSSGKPFFGGDNVGL 437
           +ID  +V    K      +KE+ +  ++T  A++ LE  L++    K FFGG+  GL
Sbjct: 103 FIDDKIVGAVSKSVFTVDEKEREKNVEETYEALQFLENELKD----KKFFGGEEFGL 155



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>LGUL_SOYBN (P46417) Lactoylglutathione lyase (EC 4.4.1.5) (Methylglyoxalase)|
           (Aldoketomutase) (Glyoxalase I)
          Length = 219

 Score = 37.7 bits (86), Expect(2) = 8e-05
 Identities = 20/56 (35%), Positives = 32/56 (57%)
 Frame = -3

Query: 607 YIDQNLVAPWWKMFVGKTDKEKHEGTKQTLAAVEKLEVALRECSSGKPFFGGDNVG 440
           YID+ +   W KM++ K  +E  EG K+ ++  ++LE  L +    KPF+G D  G
Sbjct: 101 YIDKKIYDTWKKMWLSK-GEEHEEGKKELISIFKQLEETLTD----KPFYGDDTFG 151



 Score = 27.7 bits (60), Expect(2) = 8e-05
 Identities = 15/61 (24%), Positives = 29/61 (47%)
 Frame = -1

Query: 453 ETTSGYIDVVLGGMVAWMQGTEALCGLELLDATKTPLLLAWMERFGGMEPAKAVLPDIDR 274
           + T G++D+ L    +W    E     ++ +  + P L+AW++R    E     LPD  +
Sbjct: 147 DDTFGFVDLCLITFSSWFYTYETYGNFKMEE--ECPKLMAWVKRCMERETVSNTLPDAKK 204

Query: 273 L 271
           +
Sbjct: 205 V 205



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>GSTX4_TOBAC (Q03666) Probable glutathione S-transferase (EC 2.5.1.18)|
           (Auxin-induced protein PCNT107)
          Length = 221

 Score = 34.3 bits (77), Expect(2) = 0.001
 Identities = 19/70 (27%), Positives = 31/70 (44%)
 Frame = -1

Query: 441 GYIDVVLGGMVAWMQGTEALCGLELLDATKTPLLLAWMERFGGMEPAKAVLPDIDRLVEF 262
           G++D+ L G  +W    E           + P  +AW +R    E     LPD  +++EF
Sbjct: 153 GFVDIALIGYYSWFYAYETFGNFST--EAECPKFVAWAKRCMQRESVAKSLPDQPKVLEF 210

Query: 261 AKMKRAQKAL 232
            K+ R +  L
Sbjct: 211 VKVLRQKFGL 220



 Score = 27.3 bits (59), Expect(2) = 0.001
 Identities = 19/58 (32%), Positives = 31/58 (53%)
 Frame = -3

Query: 613 AAYIDQNLVAPWWKMFVGKTDKEKHEGTKQTLAAVEKLEVALRECSSGKPFFGGDNVG 440
           A YID+ L     K++  K + E+    K  +  ++ LE AL +    KP+FGG++ G
Sbjct: 101 ADYIDKKLYDFGRKLWTTKGE-EQEAAKKDFIECLKVLEGALGD----KPYFGGESFG 153



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>GSTXC_TOBAC (P49332) Probable glutathione S-transferase parC (EC 2.5.1.18)|
           (Auxin-regulated protein parC)
          Length = 221

 Score = 34.7 bits (78), Expect = 0.23
 Identities = 19/70 (27%), Positives = 31/70 (44%)
 Frame = -1

Query: 441 GYIDVVLGGMVAWMQGTEALCGLELLDATKTPLLLAWMERFGGMEPAKAVLPDIDRLVEF 262
           G++D+ L G  +W    E           + P  +AW +R    E     LPD  +++EF
Sbjct: 153 GFVDIALIGFYSWFYAYETFGNFST--EAECPKFVAWAKRCMQRESVAKSLPDQPKVLEF 210

Query: 261 AKMKRAQKAL 232
            K+ R +  L
Sbjct: 211 VKVLRQKFGL 220



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>DAG1_BOVIN (O18738) Dystroglycan precursor (Dystrophin-associated glycoprotein|
           1) [Contains: Alpha-dystroglycan (Alpha-DG);
           Beta-dystroglycan (Beta-DG)]
          Length = 895

 Score = 32.7 bits (73), Expect = 0.87
 Identities = 22/64 (34%), Positives = 30/64 (46%), Gaps = 3/64 (4%)
 Frame = +3

Query: 192 LLLEIPILLSHFRLEPSEHVSSWQTQ---PTYQYLAELP*QVPFHQSAPSMPAVTGFWWR 362
           LLL + +  SH+  EPSE V  W+ Q     +  L++L   VP     P   AV G  +R
Sbjct: 20  LLLSVAVTQSHWPSEPSEAVRDWENQLEASMHSALSDLHETVPTVVGIPDGTAVVGRSFR 79

Query: 363 QGAP 374
              P
Sbjct: 80  VTIP 83



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>DAG1_HUMAN (Q14118) Dystroglycan precursor (Dystrophin-associated glycoprotein|
           1) [Contains: Alpha-dystroglycan (Alpha-DG);
           Beta-dystroglycan (Beta-DG)]
          Length = 895

 Score = 32.0 bits (71), Expect = 1.5
 Identities = 22/64 (34%), Positives = 30/64 (46%), Gaps = 3/64 (4%)
 Frame = +3

Query: 192 LLLEIPILLSHFRLEPSEHVSSWQTQ---PTYQYLAELP*QVPFHQSAPSMPAVTGFWWR 362
           LLL + +  SH+  EPSE V  W+ Q     +  L++L   VP     P   AV G  +R
Sbjct: 20  LLLSVVMAQSHWPSEPSEAVRDWENQLEASMHSVLSDLHEAVPTVVGIPDGTAVVGRSFR 79

Query: 363 QGAP 374
              P
Sbjct: 80  VTIP 83



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>DAG1_MOUSE (Q62165) Dystroglycan precursor (Dystrophin-associated glycoprotein|
           1) [Contains: Alpha-dystroglycan (Alpha-DG);
           Beta-dystroglycan (Beta-DG)]
          Length = 893

 Score = 31.2 bits (69), Expect = 2.5
 Identities = 20/64 (31%), Positives = 29/64 (45%), Gaps = 3/64 (4%)
 Frame = +3

Query: 192 LLLEIPILLSHFRLEPSEHVSSWQTQ---PTYQYLAELP*QVPFHQSAPSMPAVTGFWWR 362
           LLL + +  +H+  EPSE V  W+ Q     +  L++    VP     P   AV G  +R
Sbjct: 18  LLLSVAVAQAHWPSEPSEAVRDWKNQLEASMHSVLSDFQEAVPTVVGIPDGTAVVGRSFR 77

Query: 363 QGAP 374
              P
Sbjct: 78  VSIP 81



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>YL_DROME (P98163) Putative vitellogenin receptor precursor (Protein yolkless)|
            (YL)
          Length = 1984

 Score = 30.8 bits (68), Expect = 3.3
 Identities = 20/40 (50%), Positives = 26/40 (65%), Gaps = 2/40 (5%)
 Frame = +2

Query: 296  ALAGS-IPPKRSIHA-SSNGVLVASRSSRPQSASVPCIHA 409
            AL GS I  K SI+  ++  ++VA RS +PQ AS PC HA
Sbjct: 1702 ALYGSRICHKISINVLNAQDIVVAGRSRQPQKASHPCAHA 1741



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>GSTXA_TOBAC (P25317) Probable glutathione S-transferase parA (EC 2.5.1.18)|
           (Auxin-regulated protein parA) (STR246C protein)
          Length = 220

 Score = 30.8 bits (68), Expect = 3.3
 Identities = 18/58 (31%), Positives = 33/58 (56%)
 Frame = -3

Query: 613 AAYIDQNLVAPWWKMFVGKTDKEKHEGTKQTLAAVEKLEVALRECSSGKPFFGGDNVG 440
           A YID+ + +   +++ GK + ++ E  K+ +  ++ LE  L      K +FGGDN+G
Sbjct: 100 ADYIDKKIYSTGRRVWSGKGE-DQEEAKKEFIEILKTLEGEL----GNKTYFGGDNLG 152



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>DAG1_RABIT (Q28685) Dystroglycan precursor (Dystrophin-associated glycoprotein|
           1) [Contains: Alpha-dystroglycan (Alpha-DG);
           Beta-dystroglycan (Beta-DG)]
          Length = 895

 Score = 30.4 bits (67), Expect = 4.3
 Identities = 21/64 (32%), Positives = 30/64 (46%), Gaps = 3/64 (4%)
 Frame = +3

Query: 192 LLLEIPILLSHFRLEPSEHVSSWQTQ---PTYQYLAELP*QVPFHQSAPSMPAVTGFWWR 362
           LLL + +  SH+  EPSE V  W+ Q     +  L++L   +P     P   AV G  +R
Sbjct: 20  LLLCVAVAQSHWPSEPSEAVRDWENQLEASMHSVLSDLHEALPTVVGIPDGTAVVGRSFR 79

Query: 363 QGAP 374
              P
Sbjct: 80  VTIP 83



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>CTPV_MYCTU (P77894) Probable cation-transporting ATPase V (EC 3.6.3.-)|
          Length = 770

 Score = 30.4 bits (67), Expect = 4.3
 Identities = 19/67 (28%), Positives = 26/67 (38%)
 Frame = -2

Query: 521 ARSSGEARGGPKGMLQWKAVLWRRQRRATLTSCWVVWSHGCKEPRRSVAWSSLTPPKPRY 342
           +R  G  RG  +G   W     RR+RR  L   W+    G      S+ + +       Y
Sbjct: 109 SRCGGRPRGPVRGSASWPGEQNRRERRTWLPRVWLALPLGLLALGSSMFFGA-------Y 161

Query: 341 CWHGWSA 321
            W GW A
Sbjct: 162 PWAGWLA 168



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>RIMS1_HUMAN (Q86UR5) Regulating synaptic membrane exocytosis protein 1|
            (Rab3-interacting molecule 1) (RIM 1)
          Length = 1692

 Score = 30.4 bits (67), Expect = 4.3
 Identities = 18/49 (36%), Positives = 22/49 (44%), Gaps = 2/49 (4%)
 Frame = +3

Query: 363  QGAPGHRAPR--FLASMRPYHPTRRQCSPTLSPPKNGFPLEHSLRATSS 503
            QG P  R P      S+   HPTRR  SPT     +  P++H  R   S
Sbjct: 962  QGKPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPVDHRTRDVDS 1010



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>RGF3_SCHPO (Q9Y7U5) Rho1 guanine nucleotide exchange factor 3|
          Length = 1275

 Score = 29.6 bits (65), Expect = 7.3
 Identities = 22/71 (30%), Positives = 33/71 (46%)
 Frame = +2

Query: 251 FILANSTNLSISGRTALAGSIPPKRSIHASSNGVLVASRSSRPQSASVPCIHATIPPNTT 430
           + L N  +LS S    L  S P +      S   L +S + RPQS   P +    PPN  
Sbjct: 196 YSLYNDISLSCSPEPYLPLS-PTRSPARTPSPIRLYSSDALRPQSPLSPSVEYLTPPNPY 254

Query: 431 SM*PDVVSSKE 463
           S+  D+ S+++
Sbjct: 255 SLKSDISSTRQ 265



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>GSTX1_NICPL (P50471) Probable glutathione S-transferase MSR-1 (EC 2.5.1.18)|
           (Auxin-regulated protein MSR-1)
          Length = 219

 Score = 29.6 bits (65), Expect = 7.3
 Identities = 18/58 (31%), Positives = 31/58 (53%)
 Frame = -3

Query: 613 AAYIDQNLVAPWWKMFVGKTDKEKHEGTKQTLAAVEKLEVALRECSSGKPFFGGDNVG 440
           A YID  + +   +++ GK + ++ E  K+ +   + LE  L      K +FGGDN+G
Sbjct: 100 ANYIDNKIYSTGRRVWSGKGE-DQEEAKKEFIEIFKTLEGEL----GNKTYFGGDNLG 152



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>FUT4_PANTR (Q659K9) Alpha-(1,3)-fucosyltransferase (EC 2.4.1.-) (Galactoside|
           3-L-fucosyltransferase) (Fucosyltransferase 4) (FUCT-IV)
           (Fuc-TIV)
          Length = 405

 Score = 29.3 bits (64), Expect = 9.6
 Identities = 15/40 (37%), Positives = 18/40 (45%)
 Frame = +3

Query: 273 TYQYLAELP*QVPFHQSAPSMPAVTGFWWRQGAPGHRAPR 392
           TY    +LP  +P+    PS P     WW   A  H APR
Sbjct: 43  TYACWGQLP-PLPWASPTPSRPVGVLLWWEPFAGRHSAPR 81



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>V70K_OYMV (P20130) 66 kDa protein|
          Length = 597

 Score = 29.3 bits (64), Expect = 9.6
 Identities = 26/87 (29%), Positives = 44/87 (50%), Gaps = 2/87 (2%)
 Frame = +3

Query: 177 KIRSTLLLEIPILLSHFR-LEPSEHVSSWQ-TQPTYQYLAELP*QVPFHQSAPSMPAVTG 350
           ++RS  L + PIL+ HF+ L  S ++ S++  +PT + L  LP      Q  P++   T 
Sbjct: 314 RVRSNSLQQTPILMGHFKSLGQSPNLRSFERPRPTRRSLRLLPLS---PQKVPTVHVPT- 369

Query: 351 FWWRQGAPGHRAPRFLASMRPYHPTRR 431
                   GH+ P   +  RP+ P+R+
Sbjct: 370 ----HQQSGHKGP---SLPRPHSPSRQ 389



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>UBE13_WHEAT (P31252) Ubiquitin-activating enzyme E1 3|
          Length = 1053

 Score = 29.3 bits (64), Expect = 9.6
 Identities = 21/57 (36%), Positives = 30/57 (52%), Gaps = 2/57 (3%)
 Frame = -1

Query: 483 NAPVESRSLEETTSGYIDVVLGGMVAWMQGTEALCGLELLDATKTP--LLLAWMERF 319
           NA   S S+EE TS +   V GG+V  ++  + LC   L DA   P  +LL+   +F
Sbjct: 267 NARPFSFSIEEDTSNFGIYVKGGIVTQVKEPKVLCFKALRDAMTDPGEVLLSDFSKF 323



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>RIMS1_RAT (Q9JIR4) Regulating synaptic membrane exocytosis protein 1|
            (Rab3-interacting molecule 1) (RIM 1)
          Length = 1615

 Score = 29.3 bits (64), Expect = 9.6
 Identities = 18/49 (36%), Positives = 21/49 (42%), Gaps = 2/49 (4%)
 Frame = +3

Query: 363  QGAPGHRAPR--FLASMRPYHPTRRQCSPTLSPPKNGFPLEHSLRATSS 503
            QG P  R P      S+   HPTRR  SPT     +  P +H  R   S
Sbjct: 976  QGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVES 1024



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>PZRN4_HUMAN (Q6ZMN7) PDZ domain-containing RING finger protein 4 (Ligand of|
           Numb-protein X 4) (SEMACAP3-like protein)
          Length = 1036

 Score = 29.3 bits (64), Expect = 9.6
 Identities = 20/61 (32%), Positives = 28/61 (45%), Gaps = 8/61 (13%)
 Frame = -2

Query: 572 DVRGQNGQGET*RDEADARSSGEARGGPKGMLQW--------KAVLWRRQRRATLTSCWV 417
           +V  + G G T R    A   G ARGGP G  +W        + + WRR+ +A L   W 
Sbjct: 126 EVPARGGCGPTPR----AGRGGGARGGPPGG-RWGRGRGPGPRVLAWRRREKALLAQLWA 180

Query: 416 V 414
           +
Sbjct: 181 L 181



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>NODAL_HUMAN (Q96S42) Nodal homolog precursor|
          Length = 347

 Score = 29.3 bits (64), Expect = 9.6
 Identities = 26/119 (21%), Positives = 49/119 (41%), Gaps = 17/119 (14%)
 Frame = -1

Query: 378 GLELLDATKTPLLLAWMERFGGME-----------------PAKAVLPDIDRLVEFAKMK 250
           G  +L+ T+   L  W++R G +E                 PA  VL     L+ ++ + 
Sbjct: 149 GSMVLEVTRP--LSKWLKRPGALEKQMSRVAGECWPRPPTPPATNVL-----LMLYSNLS 201

Query: 249 RAQKALI*NGTTVWESQVTKYSVSSKFMSAWKECHRRHERCQNTKYCSRFSSDVSCNYV 73
           + Q+ L    T +WE++ +  +   +    W + HRRH     ++ C +    V  N +
Sbjct: 202 QEQRQLG-GSTLLWEAESSWRAQEGQLSWEWGKRHRRHHLPDRSQLCRKVKFQVDFNLI 259


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 95,003,297
Number of Sequences: 219361
Number of extensions: 2130901
Number of successful extensions: 6268
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 5948
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6265
length of database: 80,573,946
effective HSP length: 107
effective length of database: 57,102,319
effective search space used: 5538924943
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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