ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd18p03
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1UMP6_ARATH (Q9SX77) Unknown protein At1g47420, mitochondrial pre... 112 4e-25
2PHO1_ARATH (Q8S403) Putative phosphate transporter 1 33 0.23
3VHP1_CAEEL (Q10038) Tyrosine-protein phosphatase vhp-1 (EC 3.1.3... 32 0.87
4ATR_YEAST (P38111) Serine/threonine-protein kinase MEC1 (EC 2.7.... 30 2.5
5GDA2_WHEAT (P04722) Alpha/beta-gliadin A-II precursor (Prolamin) 30 2.5
6GPA8_DICDI (P34046) Guanine nucleotide-binding protein alpha-8 s... 29 4.3
7SPN90_HUMAN (Q9NZQ3) SH3 adapter protein SPIN90 (NCK-interacting... 29 5.7
8GDA9_WHEAT (P18573) Alpha/beta-gliadin MM1 precursor (Prolamin) 29 5.7
9GDA6_WHEAT (P04726) Alpha/beta-gliadin clone PW1215 precursor (P... 28 7.4
10ISTA_PSEAE (P15025) Transposase for insertion sequence element IS21 28 7.4
11PAPOA_BOVIN (P25500) Poly(A) polymerase alpha (EC 2.7.7.19) (PAP... 28 9.7
12LIP_RHIMI (P19515) Lipase precursor (EC 3.1.1.3) (Triacylglycero... 28 9.7
13RP3A_HUMAN (Q9Y2J0) Rabphilin-3A (Exophilin-1) 28 9.7
14MURD_CLOTE (Q899G5) UDP-N-acetylmuramoylalanine--D-glutamate lig... 28 9.7
15DIM_PEA (P93472) Cell elongation protein diminuto 28 9.7
16DCTS_BACHD (Q9K997) Probable C4-dicarboxylate sensor kinase (EC ... 28 9.7
17BIOX_BACSH (P22821) Protein bioX 28 9.7
18NAD2_CAEEL (P32739) Sodium-dependent high-affinity dicarboxylate... 28 9.7

>UMP6_ARATH (Q9SX77) Unknown protein At1g47420, mitochondrial precursor|
          Length = 257

 Score =  112 bits (280), Expect = 4e-25
 Identities = 52/91 (57%), Positives = 69/91 (75%)
 Frame = +3

Query: 57  RMFSSNEKHLPPISDPKIETAFKDLMAASWNELPGSLVEEAKKEVSKATDDKAGQEALEN 236
           R FS +  H+P + D  +  AFKDLMAA W ELP ++V++AK  +SK TDDKAGQEAL+N
Sbjct: 87  RRFSEDVSHMPEMEDSDVLNAFKDLMAADWAELPSAVVKDAKTAISKNTDDKAGQEALKN 146

Query: 237 VFRAAEACEEFSGVLVTLRMALDDLCGLTGE 329
           VFRAAEA EEF G+L +++M +DD  G++GE
Sbjct: 147 VFRAAEAVEEFGGILTSIKMEIDDSIGMSGE 177



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>PHO1_ARATH (Q8S403) Putative phosphate transporter 1|
          Length = 782

 Score = 33.5 bits (75), Expect = 0.23
 Identities = 19/69 (27%), Positives = 31/69 (44%)
 Frame = +1

Query: 73  MRNICLQYLTRKLRLHLRI*WLPAGMSFRVXXXXXXXXXXXXXXMIRLVKKLWKMYFVQL 252
           +RN    YL+  L L LR+ W+   M FRV              +  + +  W  Y V+ 
Sbjct: 697 LRNKNFYYLSIALNLVLRVAWIETIMRFRVSPVQSHLLDFFLASLEVIRRGHWNFYRVEN 756

Query: 253 KHVKNLVEF 279
           +H+ N+ +F
Sbjct: 757 EHLNNVGQF 765



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>VHP1_CAEEL (Q10038) Tyrosine-protein phosphatase vhp-1 (EC 3.1.3.48) (Vh1 dual|
           specificity phosphatase family protein 1)
          Length = 657

 Score = 31.6 bits (70), Expect = 0.87
 Identities = 26/87 (29%), Positives = 38/87 (43%), Gaps = 4/87 (4%)
 Frame = -1

Query: 328 SPVRP-QRSSRAILRVTKTPLNSSHASAARNT---FSKAS*PALSSVALDTSFFASSTRE 161
           SP+ P   SSR ++    TP  SS +S +      FS     + SS+ ++  FFAS+   
Sbjct: 481 SPIIPVASSSREVILTLPTPAASSSSSTSSEPSFDFSSFESSSSSSIVVENPFFASTEVP 540

Query: 160 PGSSFQLAAIKSLNAVSIFGSDIGGRC 80
            GSS       S +  +   S    RC
Sbjct: 541 AGSSSISTPSGSQSTPASASSSAASRC 567



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>ATR_YEAST (P38111) Serine/threonine-protein kinase MEC1 (EC 2.7.11.1)|
            (DNA-damage checkpoint kinase MEC1) (Mitosis entry
            checkpoint protein 1) (ATR homolog)
          Length = 2368

 Score = 30.0 bits (66), Expect = 2.5
 Identities = 19/47 (40%), Positives = 30/47 (63%), Gaps = 2/47 (4%)
 Frame = +3

Query: 102  PKIETAFKDLMAASWNELPGSLVEEAKKEVSKATDDKAG--QEALEN 236
            PK+ T + D+ AAS +E PG+     K+ +SKAT+D     +EAL++
Sbjct: 1849 PKVITFWLDIAAASISEAPGN----RKEMLSKATEDICSHVEEALQH 1891



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>GDA2_WHEAT (P04722) Alpha/beta-gliadin A-II precursor (Prolamin)|
          Length = 291

 Score = 30.0 bits (66), Expect = 2.5
 Identities = 20/66 (30%), Positives = 28/66 (42%), Gaps = 4/66 (6%)
 Frame = -2

Query: 276 LH*ILHMLQLHEIHF--PKLLDQPYHQXXXXXXXXLPQQENPEAHSSWQPS--NP*MQSQ 109
           +H ++H + LH+ H    +   Q   Q         PQQ+ P     +QPS  NP  Q  
Sbjct: 189 IHNVVHAIILHQQHHHHQQQQQQQQQQPLSQVSFQQPQQQYPSGQGFFQPSQQNPQAQGS 248

Query: 108 FSGQIL 91
           F  Q L
Sbjct: 249 FQPQQL 254



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>GPA8_DICDI (P34046) Guanine nucleotide-binding protein alpha-8 subunit (G|
           alpha-8)
          Length = 403

 Score = 29.3 bits (64), Expect = 4.3
 Identities = 15/40 (37%), Positives = 21/40 (52%)
 Frame = +3

Query: 90  PISDPKIETAFKDLMAASWNELPGSLVEEAKKEVSKATDD 209
           P+    I   +K  +  +W E  G L+EE KK + KAT D
Sbjct: 316 PLHTFHIAARYKKDIKYTWEEAKGILLEENKKVLMKATKD 355



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>SPN90_HUMAN (Q9NZQ3) SH3 adapter protein SPIN90 (NCK-interacting protein with|
           SH3 domain) (SH3 protein interacting with Nck, 90 kDa)
           (VacA-interacting protein, 54 kDa) (VIP54) (AF3p21)
           (Diaphanous protein-interacting protein)
           (Dia-interacting protein 1)
          Length = 722

 Score = 28.9 bits (63), Expect = 5.7
 Identities = 20/61 (32%), Positives = 32/61 (52%)
 Frame = -1

Query: 331 TSPVRPQRSSRAILRVTKTPLNSSHASAARNTFSKAS*PALSSVALDTSFFASSTREPGS 152
           T P   +R  R  L  + +  +++  S   +  S +S   +SS +LDT + +SS  EPGS
Sbjct: 181 TPPPPVKRRDREALMASGSGGHNTMPSGGNSVSSGSS---VSSTSLDTLYTSSSPSEPGS 237

Query: 151 S 149
           S
Sbjct: 238 S 238



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>GDA9_WHEAT (P18573) Alpha/beta-gliadin MM1 precursor (Prolamin)|
          Length = 307

 Score = 28.9 bits (63), Expect = 5.7
 Identities = 18/60 (30%), Positives = 27/60 (45%)
 Frame = -2

Query: 276 LH*ILHMLQLHEIHFPKLLDQPYHQXXXXXXXXLPQQENPEAHSSWQPSNP*MQSQFSGQ 97
           +H ++H + LH+    +   Q   Q         PQQ+ P    S+QPS    Q+Q S Q
Sbjct: 209 IHNVVHAIILHQQQQQQ--QQQQQQPLSQVSFQQPQQQYPSGQGSFQPSQQNPQAQGSVQ 266



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>GDA6_WHEAT (P04726) Alpha/beta-gliadin clone PW1215 precursor (Prolamin)|
          Length = 296

 Score = 28.5 bits (62), Expect = 7.4
 Identities = 16/54 (29%), Positives = 24/54 (44%), Gaps = 6/54 (11%)
 Frame = -2

Query: 276 LH*ILHMLQLHEIHFPKL------LDQPYHQXXXXXXXXLPQQENPEAHSSWQP 133
           +H ++H + LH+    +       L QP  Q         P Q+NP+A  S QP
Sbjct: 201 IHNVVHAIILHQQQRQQQPSSQVSLQQPQQQYPSGQGFFQPSQQNPQAQGSVQP 254



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>ISTA_PSEAE (P15025) Transposase for insertion sequence element IS21|
          Length = 390

 Score = 28.5 bits (62), Expect = 7.4
 Identities = 10/37 (27%), Positives = 20/37 (54%)
 Frame = +3

Query: 39  FSWNSRRMFSSNEKHLPPISDPKIETAFKDLMAASWN 149
           F     + F+  ++HL P+ D   +T++ D+   SW+
Sbjct: 273 FKETPEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWD 309



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>PAPOA_BOVIN (P25500) Poly(A) polymerase alpha (EC 2.7.7.19) (PAP)|
           (Polynucleotide adenylyltransferase alpha)
          Length = 738

 Score = 28.1 bits (61), Expect = 9.7
 Identities = 23/93 (24%), Positives = 45/93 (48%)
 Frame = -1

Query: 370 AIHKQGKKGD*VLTSPVRPQRSSRAILRVTKTPLNSSHASAARNTFSKAS*PALSSVALD 191
           A+H + K+   +L S V  ++   +   V  TPLN S    + ++ +  S P+ +S A+ 
Sbjct: 484 AMHVKRKQLHQLLPSHVLQKKKKHSTEGVKLTPLNDSSLDLSMDSDNSMSVPSPTS-AMK 542

Query: 190 TSFFASSTREPGSSFQLAAIKSLNAVSIFGSDI 92
           TS   SS    G +    A+ + +  +I  +++
Sbjct: 543 TSPLNSSGSSQGRNSPAPAVTAASVTNIQATEV 575



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>LIP_RHIMI (P19515) Lipase precursor (EC 3.1.1.3) (Triacylglycerol lipase)|
          Length = 363

 Score = 28.1 bits (61), Expect = 9.7
 Identities = 15/61 (24%), Positives = 29/61 (47%)
 Frame = +3

Query: 3   TFYVVYTDGNNAFSWNSRRMFSSNEKHLPPISDPKIETAFKDLMAASWNELPGSLVEEAK 182
           T Y+V+   ++  +W +   F       PP+S  K+   F D      NEL  +++++ K
Sbjct: 168 TIYIVFRGSSSIRNWIADLTFVPVS--YPPVSGTKVHKGFLDSYGEVQNELVATVLDQFK 225

Query: 183 K 185
           +
Sbjct: 226 Q 226



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>RP3A_HUMAN (Q9Y2J0) Rabphilin-3A (Exophilin-1)|
          Length = 694

 Score = 28.1 bits (61), Expect = 9.7
 Identities = 18/69 (26%), Positives = 35/69 (50%)
 Frame = +3

Query: 60  MFSSNEKHLPPISDPKIETAFKDLMAASWNELPGSLVEEAKKEVSKATDDKAGQEALENV 239
           +FS++       SD  +++  K+ + A W+  PG   +  +K+  + TD++  +E +  V
Sbjct: 5   VFSNSSNRWMYPSDRPLQSNDKEQLQAGWSVHPGGQPDRQRKQ-EELTDEE--KEIINRV 61

Query: 240 FRAAEACEE 266
              AE  EE
Sbjct: 62  IARAEKMEE 70



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>MURD_CLOTE (Q899G5) UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC|
           6.3.2.9) (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
           synthetase) (D-glutamic acid-adding enzyme)
          Length = 458

 Score = 28.1 bits (61), Expect = 9.7
 Identities = 22/69 (31%), Positives = 36/69 (52%), Gaps = 2/69 (2%)
 Frame = +3

Query: 69  SNEKHLPPISDPK--IETAFKDLMAASWNELPGSLVEEAKKEVSKATDDKAGQEALENVF 242
           S+ KHL  + D K  IE AFK +M  S  +LP S+    ++ ++ A ++  G+    +V 
Sbjct: 374 SHIKHLILLGDTKYKIEEAFKKVMRDSSEDLPISICNSIEEAINIAKEN--GESG--DVV 429

Query: 243 RAAEACEEF 269
             + AC  F
Sbjct: 430 TLSPACASF 438



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>DIM_PEA (P93472) Cell elongation protein diminuto|
          Length = 567

 Score = 28.1 bits (61), Expect = 9.7
 Identities = 16/46 (34%), Positives = 23/46 (50%)
 Frame = +1

Query: 220 KKLWKMYFVQLKHVKNLVEF*LP*EWLLMIFVV*PVRSVLNHLSSL 357
           KK+W  YFV+ +             W+L+IFVV P+   L  L+ L
Sbjct: 13  KKIWVDYFVKFR-------------WILVIFVVLPISFTLYFLTYL 45



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>DCTS_BACHD (Q9K997) Probable C4-dicarboxylate sensor kinase (EC 2.7.13.3)|
          Length = 532

 Score = 28.1 bits (61), Expect = 9.7
 Identities = 19/41 (46%), Positives = 26/41 (63%), Gaps = 1/41 (2%)
 Frame = +3

Query: 216 GQEALENVFRAAEACEEFSGVLVTLRMALDDLCG-LTGEVS 335
           G++AL+ +F   E  EEFSGV V  ++  D L G L G+VS
Sbjct: 357 GKKALQYIFDLEEEQEEFSGV-VMQKIHNDSLAGLLLGKVS 396



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>BIOX_BACSH (P22821) Protein bioX|
          Length = 166

 Score = 28.1 bits (61), Expect = 9.7
 Identities = 15/47 (31%), Positives = 25/47 (53%)
 Frame = -1

Query: 241 NTFSKAS*PALSSVALDTSFFASSTREPGSSFQLAAIKSLNAVSIFG 101
           +T+  A    L+ + + T  F   T  PGS FQL+A  ++   ++FG
Sbjct: 5   STYDLAQISLLACLIIVTGMFKIPTGIPGSEFQLSAPIAVAIAAVFG 51



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>NAD2_CAEEL (P32739) Sodium-dependent high-affinity dicarboxylate transporter 2|
           (Na(+)/dicarboxylate cotransporter 2) (NaDC-2) (ceNaDC2)
          Length = 551

 Score = 28.1 bits (61), Expect = 9.7
 Identities = 17/48 (35%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
 Frame = +3

Query: 12  VVYTDGNNAFS-WNSRRMFSSNEKHLPPISDPKIETAFKDLMAASWNE 152
           V Y  G + F+ W  R   S  E HL  + +  I+T ++DL   SW E
Sbjct: 263 VCYFMGPSTFARWFERP--SKEEAHLKKLIEKNIQTMYEDLGDVSWGE 308


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 61,059,522
Number of Sequences: 219361
Number of extensions: 1135166
Number of successful extensions: 3325
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 3246
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3325
length of database: 80,573,946
effective HSP length: 101
effective length of database: 58,418,485
effective search space used: 2511994855
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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