ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | rbasd19d17 |
|---|---|
| Clone Library Name | barley_pub |
>MCE1_SCHPO (P40997) mRNA capping enzyme alpha subunit (mRNA| guanylyltransferase) (EC 2.7.7.50) (GTP--RNA guanylyltransferase) (GTase) Length = 402 Score = 62.8 bits (151), Expect = 1e-09 Identities = 48/160 (30%), Positives = 80/160 (50%), Gaps = 17/160 (10%) Frame = -1 Query: 696 LIFQGWDDPYVNRTHEGLLKWKYPEMNSVDFLF-------ETGSENRQLVFLYERG---K 547 LIF + PYV+ T + LLKWK EMN++DF+ E G + + ++ G Sbjct: 221 LIFTCTETPYVSGTDQSLLKWKPKEMNTIDFMLKLEFAQPEEGDIDYSAMPEFQLGVWEG 280 Query: 546 KKLMDGTRIVFPEDVEQSS-------VSGKIVECSWNKQEDCWFCMRIRADKSTPNDINT 388 + + ++ ++ E +S +IVEC + E+ W +R R DK N I+T Sbjct: 281 RNMYSFFAFMYVDEKEWEKLKSFNVPLSERIVEC-YLDDENRWRFLRFRDDKRDANHIST 339 Query: 387 YRKVMRSITDNITEDKLLAEMNEISSLPMYADRKAHADRK 268 + V++SI D ++++ LL EM I R+A+ +RK Sbjct: 340 VKSVLQSIEDGVSKEDLLKEMPII--------REAYYNRK 371
>MCE1_YARLI (Q6C783) mRNA capping enzyme alpha subunit (mRNA| guanylyltransferase) (EC 2.7.7.50) (GTP--RNA guanylyltransferase) (GTase) Length = 391 Score = 58.9 bits (141), Expect = 1e-08 Identities = 58/185 (31%), Positives = 82/185 (44%), Gaps = 33/185 (17%) Frame = -1 Query: 696 LIFQGWDDPYVNRTHEGLLKWKYPEMNSVDFLF--------ETGSEN-------RQLVFL 562 LIF D PYV+ T E +LKWK + NSVDFL +T E+ ++ L Sbjct: 212 LIFTCRDHPYVSGTDERILKWKKQDENSVDFLMTMKFPIFEDTNGESWTDYDAKPEITLL 271 Query: 561 YERGKKKLMDGTR---IVFPEDVE-------QSSVSGKIVECSWNKQEDCWFCMRIRADK 412 G+ DG+R ++ D E + + ++VEC + ++ W +R R DK Sbjct: 272 VWTGR----DGSRPYGELYLTDEEWDNLKALEEPLEERVVECIKDDKKR-WRYLRFRDDK 326 Query: 411 STPNDINTYRKVMRSITDNITEDKLLAEMNEISSL-------PMYADRK-AHADRKAHAE 256 + N I T KV+ SI D ++E LL +I L P DRK D H Sbjct: 327 TNANYITTVEKVIDSIDDPVSEKNLLDAAPKIKELWKERNRRPRDEDRKRVGGDDHDHGA 386 Query: 255 KMAHQ 241 K A Q Sbjct: 387 KRARQ 391 Score = 31.6 bits (70), Expect = 2.4 Identities = 13/34 (38%), Positives = 22/34 (64%) Frame = -2 Query: 353 LLKTSSWPR*MKSVAFRCMPIGKLMLTGRPMPRR 252 L+ T + PR + + F C+ K++LTGRP+ +R Sbjct: 121 LVLTKAEPRSLHFLMFDCLACNKILLTGRPLDKR 154
>MCE1_HUMAN (O60942) mRNA capping enzyme (HCE) (HCAP1) [Includes:| Polynucleotide 5'-triphosphatase (EC 3.1.3.33) (mRNA 5'-triphosphatase) (TPase); mRNA guanylyltransferase (EC 2.7.7.50) (GTP--RNA guanylyltransferase) (GTase)] Length = 597 Score = 58.9 bits (141), Expect = 1e-08 Identities = 40/132 (30%), Positives = 63/132 (47%), Gaps = 6/132 (4%) Frame = -1 Query: 651 EGLLKWKYPEMNSVDF---LFETGSEN---RQLVFLYERGKKKLMDGTRIVFPEDVEQSS 490 + +LKWK P +NSVDF + G E + + LY G ++ ++ E Sbjct: 454 DDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIKVT----KELKQ 509 Query: 489 VSGKIVECSWNKQEDCWFCMRIRADKSTPNDINTYRKVMRSITDNITEDKLLAEMNEISS 310 KI+EC + + + W MR R DKS PN NT V SI++ +T++ L ++ ++ Sbjct: 510 YDNKIIECKF--ENNSWVFMRQRTDKSFPNAYNTAMAVCNSISNPVTKEMLFEFIDRCTA 567 Query: 309 LPMYADRKAHAD 274 RK H D Sbjct: 568 ASQGQKRKHHLD 579
>MCE1_MOUSE (O55236) mRNA capping enzyme (HCE) (MCE1) [Includes: Polynucleotide| 5'-triphosphatase (EC 3.1.3.33) (mRNA 5'-triphosphatase) (TPase); mRNA guanylyltransferase (EC 2.7.7.50) (GTP--RNA guanylyltransferase) (GTase)] Length = 597 Score = 54.7 bits (130), Expect = 3e-07 Identities = 39/132 (29%), Positives = 62/132 (46%), Gaps = 6/132 (4%) Frame = -1 Query: 651 EGLLKWKYPEMNSVDF---LFETGSEN---RQLVFLYERGKKKLMDGTRIVFPEDVEQSS 490 + +LKWK P +NSVDF + G E + + LY G ++ ++ E Sbjct: 454 DDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIKVT----KELKQ 509 Query: 489 VSGKIVECSWNKQEDCWFCMRIRADKSTPNDINTYRKVMRSITDNITEDKLLAEMNEISS 310 KI+EC + + + W MR R DKS PN NT V SI++ +T++ L ++ ++ Sbjct: 510 YDNKIIECKF--ENNSWVFMRQRIDKSFPNAYNTAMAVCNSISNPVTKEMLFEFIDRCAA 567 Query: 309 LPMYADRKAHAD 274 RK D Sbjct: 568 AAQGQKRKYPLD 579
>MCE1_CANAL (P78587) mRNA capping enzyme alpha subunit (mRNA| guanylyltransferase) (EC 2.7.7.50) (GTP--RNA guanylyltransferase) (GTase) Length = 449 Score = 50.1 bits (118), Expect = 7e-06 Identities = 48/172 (27%), Positives = 75/172 (43%), Gaps = 29/172 (16%) Frame = -1 Query: 696 LIFQGWDDPYVNRTHEGLLKWKYPEMNSVDFLFETGSENRQLVFLYERG----------- 550 LI+ + PYV T + LLKWK E N+VDF E Q L ER Sbjct: 222 LIYTCAETPYVFGTDQTLLKWKPAEENTVDFQLEFVFNEVQDPDLDERDPTSTYLDYDAK 281 Query: 549 ----KKKLMDGTRI--------VFPEDVE-----QSSVSGKIVECSWN-KQEDCWFCMRI 424 K ++ G+ + + +D E + + G+I EC + ++ W +R Sbjct: 282 PNLIKLRVWQGSNVHTDFAKLDLSDDDWERLKALEQPLQGRIAECRQSTTKKGYWEMLRF 341 Query: 423 RADKSTPNDINTYRKVMRSITDNITEDKLLAEMNEISSLPMYADRKAHADRK 268 R DKS N I+ K++ SI D + E +++ +IS A +K DR+ Sbjct: 342 RNDKSNGNHISVVEKILVSIKDGVKEKEVIEWCPKISR----AWKKRENDRR 389
>MCE1_DEBHA (Q6BT58) mRNA capping enzyme alpha subunit (mRNA| guanylyltransferase) (EC 2.7.7.50) (GTP--RNA guanylyltransferase) (GTase) Length = 458 Score = 48.5 bits (114), Expect = 2e-05 Identities = 42/150 (28%), Positives = 63/150 (42%), Gaps = 29/150 (19%) Frame = -1 Query: 696 LIFQGWDDPYVNRTHEGLLKWKYPEMNSVDFLFET----------------------GSE 583 LIF + PYV T LLKWK N+VD+ E S+ Sbjct: 221 LIFTCAETPYVFGTDSTLLKWKPAHENTVDYKMEMIFKKFQDPDLDPRDPDSTYTDYDSK 280 Query: 582 NRQLVFLYERGKKKLMDGTRIVFP-EDVE-----QSSVSGKIVECSWNKQE-DCWFCMRI 424 + +G D T++ ED E + + G+IVEC + W +R Sbjct: 281 PELIKLRVWKGGADYEDFTKLSLENEDWEKLKNLRQPLQGRIVECRKKLSDPGFWEMLRF 340 Query: 423 RADKSTPNDINTYRKVMRSITDNITEDKLL 334 R DKS N I+ K++ SI D ++E++L+ Sbjct: 341 RNDKSNGNHISVVDKILHSIQDGVSEEELI 370
>MCE1_CAEEL (Q17607) mRNA capping enzyme [Includes: Polynucleotide| 5'-triphosphatase (EC 3.1.3.33) (mRNA 5'-triphosphatase) (TPase); mRNA guanylyltransferase (EC 2.7.7.50) (GTP--RNA guanylyltransferase) (GTase)] Length = 623 Score = 47.8 bits (112), Expect = 3e-05 Identities = 44/158 (27%), Positives = 68/158 (43%), Gaps = 9/158 (5%) Frame = -1 Query: 696 LIFQGWDDPYVNRTHEGLLKWKYPEMNSVDFLFETGSENRQLVFLYERGK---KKLMD-- 532 LIFQ Y + +LKWK P NSVDFL + + ++ + G + L D Sbjct: 458 LIFQPKKTKYETGRCDKVLKWKPPSHNSVDFLLKVEKKCKEGMLPEWIGYLFVQNLSDPF 517 Query: 531 GTRIVFPEDVEQSSVSGKIVECSW----NKQEDCWFCMRIRADKSTPNDINTYRKVMRSI 364 GT KI+EC+ + W MR R DKS PN + T V+ ++ Sbjct: 518 GT---MKATATLKKYHNKIIECTLLVDNQGRPKEWKFMRERTDKSLPNGLRTAENVVETM 574 Query: 363 TDNITEDKLLAEMNEISSLPMYADRKAHADRKAHAEKM 250 + +TE L+ +N + A AH + H +++ Sbjct: 575 VNPVTETYLIEYVNHALRVLKRA-AAAHRHHQIHQQQL 611
>MCE1_CANGA (Q6FQ31) mRNA capping enzyme alpha subunit (mRNA| guanylyltransferase) (EC 2.7.7.50) (GTP--RNA guanylyltransferase) (GTase) Length = 449 Score = 47.4 bits (111), Expect = 4e-05 Identities = 22/63 (34%), Positives = 37/63 (58%) Frame = -1 Query: 498 QSSVSGKIVECSWNKQEDCWFCMRIRADKSTPNDINTYRKVMRSITDNITEDKLLAEMNE 319 + ++G+IVEC+ + + W +R R DK N + +KV+ SI+D++T D L + E Sbjct: 340 EEPLNGRIVECTKDPETGSWTMLRFRDDKLNGNHTSVVQKVLESISDSVTIDDLAESVPE 399 Query: 318 ISS 310 I S Sbjct: 400 IKS 402
>MCE1_KLULA (Q6CWR0) mRNA capping enzyme alpha subunit (mRNA| guanylyltransferase) (EC 2.7.7.50) (GTP--RNA guanylyltransferase) (GTase) Length = 466 Score = 46.6 bits (109), Expect = 7e-05 Identities = 21/63 (33%), Positives = 39/63 (61%) Frame = -1 Query: 498 QSSVSGKIVECSWNKQEDCWFCMRIRADKSTPNDINTYRKVMRSITDNITEDKLLAEMNE 319 + ++G+IVECS +++ W +R R DK N ++ +KV+ SI D+++ D L ++E Sbjct: 341 EEPLNGRIVECSKDQESGAWKLLRFRDDKLNGNHVSVVQKVLESIGDSVSLDDLEQVVDE 400 Query: 318 ISS 310 + S Sbjct: 401 MRS 403
>MCE1_YEAST (Q01159) mRNA capping enzyme alpha subunit (mRNA| guanylyltransferase) (EC 2.7.7.50) (GTP--RNA guanylyltransferase) (GTase) Length = 459 Score = 42.7 bits (99), Expect = 0.001 Identities = 18/54 (33%), Positives = 33/54 (61%) Frame = -1 Query: 498 QSSVSGKIVECSWNKQEDCWFCMRIRADKSTPNDINTYRKVMRSITDNITEDKL 337 + ++G+IVEC+ N++ W +R R DK N + +KV+ SI D+++ + L Sbjct: 344 EQPLNGRIVECAKNQETGAWEMLRFRDDKLNGNHTSVVQKVLESINDSVSLEDL 397
>MCE_PBCV1 (Q84424) mRNA capping enzyme (mRNA guanylyltransferase) (EC| 2.7.7.50) (GTP--RNA guanylyltransferase) Length = 330 Score = 42.0 bits (97), Expect = 0.002 Identities = 35/130 (26%), Positives = 58/130 (44%), Gaps = 9/130 (6%) Frame = -1 Query: 696 LIFQGWDDPYVNRTHEGLLKWKYPEMNSVDFLFETGSENRQLVFLYERGKKKLMDGTRIV 517 LI D+P + + L K K +++DF+ + DGT + Sbjct: 215 LIIMSVDEPVIYGRNFNLFKLKPGTHHTIDFIIMSE------------------DGTIGI 256 Query: 516 FPEDVEQSSVSGK---------IVECSWNKQEDCWFCMRIRADKSTPNDINTYRKVMRSI 364 F ++ ++ GK IVEC + + W ++ R+DK+ ND TY K + +I Sbjct: 257 FDPNLRKNVPVGKLDGYYNKGSIVECGF--ADGTWKYIQGRSDKNQANDRLTYEKTLLNI 314 Query: 363 TDNITEDKLL 334 +NIT D+LL Sbjct: 315 EENITIDELL 324
>MCE1_ASHGO (Q755D0) mRNA capping enzyme alpha subunit (mRNA| guanylyltransferase) (EC 2.7.7.50) (GTP--RNA guanylyltransferase) (GTase) Length = 463 Score = 40.8 bits (94), Expect = 0.004 Identities = 42/171 (24%), Positives = 70/171 (40%), Gaps = 51/171 (29%) Frame = -1 Query: 696 LIFQGWDDPY-VNRTHEGLLKWKYPEMNSVDF---------------------------- 604 LIF D PY V LLKWK + N+VDF Sbjct: 223 LIFTAVDTPYTVGGKDSTLLKWKPEQENTVDFKMILEIPVVEDDTLPKKDRNRFYYNFDV 282 Query: 603 -------LFETGSENRQLVFLYER--GKKKL--MDGTRIVFPE-----------DVEQSS 490 +++ G + + +E+ KK+L + T VF E + Sbjct: 283 KPSFHLYIWQGGPDVNTRLHDFEQPFSKKELEILHRTYKVFAELQISDEQWAKMKALEQP 342 Query: 489 VSGKIVECSWNKQEDCWFCMRIRADKSTPNDINTYRKVMRSITDNITEDKL 337 ++G+IVEC+ +++ W +R R DK N ++ +KV+ SI+D++ + L Sbjct: 343 LNGRIVECAKDQETGEWKFLRFRDDKLNGNHVSVVQKVLESISDSVKLEDL 393
>PTN22_MOUSE (P29352) Tyrosine-protein phosphatase non-receptor type 22 (EC| 3.1.3.48) (Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP) Length = 802 Score = 31.2 bits (69), Expect = 3.1 Identities = 15/28 (53%), Positives = 18/28 (64%) Frame = +2 Query: 461 QEHSTIFPETEDCSTSSGNTIRVPSISF 544 Q ST +PET + STSS T+R P SF Sbjct: 714 QTSSTSYPETTENSTSSKQTLRTPGKSF 741
>Y474_MYCPN (P75310) Hypothetical protein MG328 homolog (P01_orf1033)| Length = 1033 Score = 30.0 bits (66), Expect = 7.0 Identities = 35/154 (22%), Positives = 62/154 (40%), Gaps = 10/154 (6%) Frame = -1 Query: 663 NRTHEGLLKWKYPEMNSVDFLFETGSENRQL--------VFLYERGKKKLMDGTRIVFPE 508 NRT + L+ PE N ++F+ E E Q + Y+ KK + ++ Sbjct: 435 NRTLKQHLQNNLPEANQLNFVLEKQLEQLQQDKHSLTLQIEQYKFDSKKHQEQLALIPSL 494 Query: 507 DVEQSSVSGKIVECSWNKQEDCWFCMRIRADKSTPNDINTYRKVMRS--ITDNITEDKLL 334 E +S+ +++ Q R+ + N + T K +R + D T+ + L Sbjct: 495 RSEINSLETEVISLKQTNQ-------RLSLIERENNFLKTEIKQLRETKLNDENTKYRNL 547 Query: 333 AEMNEISSLPMYADRKAHADRKAHAEKMAHQHRR 232 + E+ M AD A H + +AHQH + Sbjct: 548 LKQYEL----MRADSDAKLKELEHEQHLAHQHHQ 577
>BT1A1_HUMAN (Q13410) Butyrophilin subfamily 1 member A1 precursor (BT)| Length = 526 Score = 30.0 bits (66), Expect = 7.0 Identities = 16/49 (32%), Positives = 26/49 (53%) Frame = -1 Query: 642 LKWKYPEMNSVDFLFETGSENRQLVFLYERGKKKLMDGTRIVFPEDVEQ 496 LKWK +++VD + + + L FLYE K ++ +R PE E+ Sbjct: 293 LKWKKATLHAVDVTLDPDTAHPHL-FLYEDSKSVRLEDSRQKLPEKTER 340
>TSP3_BRARE (Q8JHW2) Thrombospondin-3 precursor (Thbs3)| Length = 962 Score = 30.0 bits (66), Expect = 7.0 Identities = 26/92 (28%), Positives = 47/92 (51%), Gaps = 4/92 (4%) Frame = -1 Query: 642 LKWKYPEMNSVDFLFETGSENRQLVFLYERG--KKKLMDGTRIVFPEDV--EQSSVSGKI 475 L WK P +V +L +T S QL + G + KL +G+ +V DV + S G++ Sbjct: 866 LLWKDPR--NVGWLDKT-SYRWQLSHRPQVGYIRVKLYEGSEMVADSDVVIDTSMRGGRL 922 Query: 474 VECSWNKQEDCWFCMRIRADKSTPNDINTYRK 379 ++++ W +R R + + P D +++RK Sbjct: 923 GVFCFSQENIIWSNLRYRCNDTVPEDFSSHRK 954 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 97,400,537 Number of Sequences: 219361 Number of extensions: 1975289 Number of successful extensions: 5574 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 5403 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 5569 length of database: 80,573,946 effective HSP length: 109 effective length of database: 56,663,597 effective search space used: 6912958834 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
