ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | rbasd19a02 |
|---|---|
| Clone Library Name | barley_pub |
>3HIDH_ARATH (Q9SUC0) Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial| precursor (EC 1.1.1.31) (HIBADH) Length = 347 Score = 182 bits (462), Expect = 5e-46 Identities = 87/120 (72%), Positives = 100/120 (83%) Frame = -3 Query: 550 AISMLGVSEAFALGQNLGIKASTLTDIFNCSSARCWSSDTYNPVPGVMAGVPSARNYDGG 371 A+SMLG SEA ALGQ+LGI ASTLT++ N SS RCWSSD YNPVPGVM GVPS+R+Y+GG Sbjct: 227 AVSMLGTSEALALGQSLGISASTLTEVLNTSSGRCWSSDAYNPVPGVMKGVPSSRDYNGG 286 Query: 370 FTSKLMAKDLDLAMASASGVGFKCPMGSEALEIYRKLCDEGCEFKDFSCAFRHFYTGKDE 191 F SKLMAKDL+LA ASA VG K P+ S+A EIY+K+C+EG E KDFSC FRHFY GKDE Sbjct: 287 FASKLMAKDLNLAAASAEEVGHKSPLISKAQEIYKKMCEEGHETKDFSCVFRHFYNGKDE 346
>3HIDH_HUMAN (P31937) 3-hydroxyisobutyrate dehydrogenase, mitochondrial| precursor (EC 1.1.1.31) (HIBADH) Length = 336 Score = 123 bits (309), Expect = 3e-28 Identities = 64/112 (57%), Positives = 74/112 (66%) Frame = -3 Query: 550 AISMLGVSEAFALGQNLGIKASTLTDIFNCSSARCWSSDTYNPVPGVMAGVPSARNYDGG 371 AISM+G +EA LG LG+ L I N SS RCWSSDTYNPVPGVM GVPSA NY GG Sbjct: 217 AISMIGTAEAMNLGIRLGLDPKLLAKILNMSSGRCWSSDTYNPVPGVMDGVPSANNYQGG 276 Query: 370 FTSKLMAKDLDLAMASASGVGFKCPMGSEALEIYRKLCDEGCEFKDFSCAFR 215 F + LMAKDL LA SA+ +GS A +IYR +C +G KDFS F+ Sbjct: 277 FGTTLMAKDLGLAQDSATSTKSPILLGSLAHQIYRMMCAKGYSKKDFSSVFQ 328
>3HIDH_RAT (P29266) 3-hydroxyisobutyrate dehydrogenase, mitochondrial| precursor (EC 1.1.1.31) (HIBADH) Length = 335 Score = 122 bits (305), Expect = 9e-28 Identities = 62/113 (54%), Positives = 74/113 (65%) Frame = -3 Query: 550 AISMLGVSEAFALGQNLGIKASTLTDIFNCSSARCWSSDTYNPVPGVMAGVPSARNYDGG 371 AISM+G +EA LG G+ L I N SS RCWSSDTYNPVPGVM GVPS+ NY GG Sbjct: 216 AISMIGTAEAMNLGIRSGLDPKLLAKILNMSSGRCWSSDTYNPVPGVMDGVPSSNNYQGG 275 Query: 370 FTSKLMAKDLDLAMASASGVGFKCPMGSEALEIYRKLCDEGCEFKDFSCAFRH 212 F + LMAKDL LA SA+ +GS A +IYR +C +G KDFS F++ Sbjct: 276 FGTTLMAKDLGLAQDSATSTKTPILLGSVAHQIYRMMCSKGYSKKDFSSVFQY 328
>3HIDH_MOUSE (Q99L13) 3-hydroxyisobutyrate dehydrogenase, mitochondrial| precursor (EC 1.1.1.31) (HIBADH) Length = 335 Score = 121 bits (304), Expect = 1e-27 Identities = 62/113 (54%), Positives = 74/113 (65%) Frame = -3 Query: 550 AISMLGVSEAFALGQNLGIKASTLTDIFNCSSARCWSSDTYNPVPGVMAGVPSARNYDGG 371 AISM+G +EA LG G+ L I N SS RCWSSDTYNPVPGVM GVPS+ NY GG Sbjct: 216 AISMIGTAEAMNLGIRSGLDPKLLAKILNMSSGRCWSSDTYNPVPGVMHGVPSSNNYQGG 275 Query: 370 FTSKLMAKDLDLAMASASGVGFKCPMGSEALEIYRKLCDEGCEFKDFSCAFRH 212 F + LMAKDL LA SA+ +GS A +IYR +C +G KDFS F++ Sbjct: 276 FGTTLMAKDLGLAQDSATSTKTPILLGSLAHQIYRMMCSKGYSKKDFSSVFQY 328
>3HIDH_DROME (Q9V8M5) Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial| precursor (EC 1.1.1.31) (HIBADH) Length = 324 Score = 109 bits (272), Expect = 6e-24 Identities = 53/111 (47%), Positives = 71/111 (63%) Frame = -3 Query: 550 AISMLGVSEAFALGQNLGIKASTLTDIFNCSSARCWSSDTYNPVPGVMAGVPSARNYDGG 371 AISM+GVSEA L G+ A+ +I N S+ RCW+S+ YNPVPGV P+ R+Y GG Sbjct: 204 AISMIGVSEAMNLAVRQGLDANVFAEIINSSTGRCWASEIYNPVPGVCPSAPANRDYAGG 263 Query: 370 FTSKLMAKDLDLAMASASGVGFKCPMGSEALEIYRKLCDEGCEFKDFSCAF 218 F+S L+ KDL LA A+ P+GS A ++Y+ LCD+G KDFS + Sbjct: 264 FSSALITKDLGLASGVANASNSPIPLGSLAHKVYQSLCDKGLGNKDFSVVY 314
>3HIDH_CAEEL (Q9XTI0) Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial| (EC 1.1.1.31) (HIBADH) Length = 299 Score = 105 bits (262), Expect = 9e-23 Identities = 55/112 (49%), Positives = 70/112 (62%), Gaps = 1/112 (0%) Frame = -3 Query: 547 ISMLGVSEAFALGQNLGIKASTLTDIFNCSSARCWSSDTYNPVPGVMAGVPSARNYDGGF 368 I M+ V+E LG ++G+ A L I N SS RCWSSDTYNPVPGV+ +PS R Y GGF Sbjct: 181 IQMVAVAETMNLGISMGLDAKALAGIVNTSSGRCWSSDTYNPVPGVIENIPSCRGYAGGF 240 Query: 367 TSKLMAKDLDLAMASASGVGFKCPMGSEALEIYRKLC-DEGCEFKDFSCAFR 215 + LMAKDL LA +++ PMGS A +IYR L D + KDF ++ Sbjct: 241 GTTLMAKDLSLAQNASTNTQAPTPMGSLAHQIYRILARDPQYQAKDFGVVYQ 292
>MMSB_MYCTU (P63935) Probable 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31)| (HIBADH) Length = 294 Score = 80.5 bits (197), Expect = 3e-15 Identities = 39/108 (36%), Positives = 62/108 (57%) Frame = -3 Query: 550 AISMLGVSEAFALGQNLGIKASTLTDIFNCSSARCWSSDTYNPVPGVMAGVPSARNYDGG 371 A+ + ++EAF L + LG+ A +L D+ ++ CW+ T PVPG + P+ ++ G Sbjct: 176 AVQQIAIAEAFVLAEKLGLSAQSLFDVITGATGNCWAVHTNCPVPGPVPTSPANNDFKPG 235 Query: 370 FTSKLMAKDLDLAMASASGVGFKCPMGSEALEIYRKLCDEGCEFKDFS 227 F++ LM KDL LAM + + G P+GS A +IY K + + DFS Sbjct: 236 FSTALMNKDLGLAMDAVAATGATAPLGSHAADIYAKFAADHADL-DFS 282
>MMSB_MYCBO (P63936) Probable 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31)| (HIBADH) Length = 294 Score = 80.5 bits (197), Expect = 3e-15 Identities = 39/108 (36%), Positives = 62/108 (57%) Frame = -3 Query: 550 AISMLGVSEAFALGQNLGIKASTLTDIFNCSSARCWSSDTYNPVPGVMAGVPSARNYDGG 371 A+ + ++EAF L + LG+ A +L D+ ++ CW+ T PVPG + P+ ++ G Sbjct: 176 AVQQIAIAEAFVLAEKLGLSAQSLFDVITGATGNCWAVHTNCPVPGPVPTSPANNDFKPG 235 Query: 370 FTSKLMAKDLDLAMASASGVGFKCPMGSEALEIYRKLCDEGCEFKDFS 227 F++ LM KDL LAM + + G P+GS A +IY K + + DFS Sbjct: 236 FSTALMNKDLGLAMDAVAATGATAPLGSHAADIYAKFAADHADL-DFS 282
>MMSB_PSEAE (P28811) 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) (HIBADH)| Length = 298 Score = 80.1 bits (196), Expect = 4e-15 Identities = 47/120 (39%), Positives = 66/120 (55%), Gaps = 2/120 (1%) Frame = -3 Query: 547 ISMLGVSEAFALGQNLGIKASTLTDIFNCSSARCWSSDTYNPVPGVMAGVPSARNYDGGF 368 I M G +EA ALG G+ + L+++ SS W+ + YNP PGVM P++ Y GGF Sbjct: 180 ILMAGTAEALALGVKNGLDPAVLSEVMKQSSGGNWALNLYNPWPGVMPQAPASNGYAGGF 239 Query: 367 TSKLMAKDLDLAMASASGVGFKCPMGSEALEIY--RKLCDEGCEFKDFSCAFRHFYTGKD 194 +LM KDL LA+A+A V P+G+ A ++ D E DFS + + Y GKD Sbjct: 240 QVRLMNKDLGLALANAQAVQASTPLGALARNLFSLHAQADAEHEGLDFS-SIQKLYRGKD 298
>YKWC_BACSU (O34948) Hypothetical oxidoreductase ykwC (EC 1.1.-.-)| Length = 288 Score = 46.6 bits (109), Expect = 5e-05 Identities = 32/103 (31%), Positives = 49/103 (47%) Frame = -3 Query: 550 AISMLGVSEAFALGQNLGIKASTLTDIFNCSSARCWSSDTYNPVPGVMAGVPSARNYDGG 371 A M+GV+EA A Q G++ + +A WS N P ++ G N++ G Sbjct: 180 AAGMIGVAEAMAYAQKSGLEPENVLKSITTGAAGSWSLS--NLAPRMLQG-----NFEPG 232 Query: 370 FTSKLMAKDLDLAMASASGVGFKCPMGSEALEIYRKLCDEGCE 242 F K KD+ +A+ A +G + P S A +Y KL +G E Sbjct: 233 FYVKHFIKDMGIALEEAELMGEEMPGLSLAKSLYDKLAAQGEE 275
>GARR_ECOLI (P0ABQ2) 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60)| (Tartronate semialdehyde reductase) (TSAR) Length = 294 Score = 35.0 bits (79), Expect = 0.14 Identities = 29/108 (26%), Positives = 47/108 (43%) Frame = -3 Query: 550 AISMLGVSEAFALGQNLGIKASTLTDIFNCSSARCWSSDTYNPVPGVMAGVPSARNYDGG 371 A+++ +SEA L G+ + A D P+ VM RN+ G Sbjct: 178 ALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPM--VMD-----RNFKPG 230 Query: 370 FTSKLMAKDLDLAMASASGVGFKCPMGSEALEIYRKLCDEGCEFKDFS 227 F L KDL A+ ++ GVG + P+ + +E+ + L +G D S Sbjct: 231 FRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHS 278
>GARR_ECOL6 (P0ABQ3) 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60)| (Tartronate semialdehyde reductase) (TSAR) Length = 294 Score = 35.0 bits (79), Expect = 0.14 Identities = 29/108 (26%), Positives = 47/108 (43%) Frame = -3 Query: 550 AISMLGVSEAFALGQNLGIKASTLTDIFNCSSARCWSSDTYNPVPGVMAGVPSARNYDGG 371 A+++ +SEA L G+ + A D P+ VM RN+ G Sbjct: 178 ALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPM--VMD-----RNFKPG 230 Query: 370 FTSKLMAKDLDLAMASASGVGFKCPMGSEALEIYRKLCDEGCEFKDFS 227 F L KDL A+ ++ GVG + P+ + +E+ + L +G D S Sbjct: 231 FRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHS 278
>DEF_MYCLE (Q9CBI2) Peptide deformylase (EC 3.5.1.88) (PDF) (Polypeptide| deformylase) Length = 197 Score = 30.8 bits (68), Expect = 2.7 Identities = 12/20 (60%), Positives = 14/20 (70%) Frame = +3 Query: 390 ADGTPAITPGTGLYVSLLQH 449 ADG P T GTGL+ +LQH Sbjct: 129 ADGNPVTTEGTGLFARMLQH 148
>THII_OCEIH (Q8EPB3) Probable thiamine biosynthesis protein thiI| Length = 400 Score = 30.4 bits (67), Expect = 3.5 Identities = 18/46 (39%), Positives = 21/46 (45%) Frame = +1 Query: 79 QTLSGHCSTNT*IQSIGEHYCFISTSVEFAGIATGITSHPCRCRSG 216 Q L GH NT ++ H + VE AT ITS RCR G Sbjct: 125 QILGGHLLANTNDITVNVHEPDVEVRVEIRKEATYITSGTIRCRGG 170
>PPZ2_YEAST (P33329) Serine/threonine-protein phosphatase PP-Z2 (EC 3.1.3.16)| Length = 709 Score = 30.0 bits (66), Expect = 4.5 Identities = 17/37 (45%), Positives = 20/37 (54%), Gaps = 3/37 (8%) Frame = -1 Query: 216 ATSTPARMRSD---PCSYPSKLNTGRNKTIMFSNRLN 115 +TS+ R RSD P SYP G N + FSNR N Sbjct: 214 STSSKLRRRSDNTLPASYPLNAEAGGNGSDYFSNRSN 250
>QUEA_PASMU (P57832) S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC| 5.-.-.-) (Queuosine biosynthesis protein queA) Length = 363 Score = 29.6 bits (65), Expect = 5.9 Identities = 19/70 (27%), Positives = 34/70 (48%), Gaps = 11/70 (15%) Frame = -3 Query: 430 YNPVPGVMAGVPSARNYDGGFTSKLMAKDLDLAMASAS-GVGFKCP----------MGSE 284 YN VPG +A + ++D ++L AK ++ A + G G P M +E Sbjct: 175 YNKVPGAVAAPTAGLHFDDALLAQLKAKGVNFAFVTLHVGAGTFQPVRVEQIEDHKMHAE 234 Query: 283 ALEIYRKLCD 254 +E+ +++CD Sbjct: 235 YVEVPQEVCD 244
>VE1_HPV70 (P50765) Replication protein E1 (EC 3.6.1.-) (ATP-dependent| helicase E1) Length = 652 Score = 29.3 bits (64), Expect = 7.8 Identities = 15/39 (38%), Positives = 20/39 (51%) Frame = -3 Query: 304 KCPMGSEALEIYRKLCDEGCEFKDFSCAFRHFYTGKDEK 188 KC G + I + L +G EF F CAF+ F G +K Sbjct: 434 KCDDGGDWRPIVQFLRYQGVEFITFLCAFKEFLKGTPKK 472 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 79,872,022 Number of Sequences: 219361 Number of extensions: 1650505 Number of successful extensions: 3906 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 3821 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 3906 length of database: 80,573,946 effective HSP length: 105 effective length of database: 57,541,041 effective search space used: 4488201198 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
