ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd18e04
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1IF3C_ORYSA (Q94HF1) Eukaryotic translation initiation factor 3 s... 271 1e-72
2IF3C_ARATH (Q9SZA3) Eukaryotic translation initiation factor 3 s... 217 2e-56
3IF3C_BOVIN (Q3T0V3) Eukaryotic translation initiation factor 3 s... 64 3e-10
4IF3C_MOUSE (Q9DBZ5) Eukaryotic translation initiation factor 3 s... 64 4e-10
5IF3C_HUMAN (Q9UBQ5) Eukaryotic translation initiation factor 3 s... 64 4e-10
6IF3C_DROME (Q9W2D9) Probable eukaryotic translation initiation f... 54 4e-07
7IF3C_CAEEL (Q9XUP3) Probable eukaryotic translation initiation f... 38 0.025
8IF3C_CAEBR (Q61K76) Probable eukaryotic translation initiation f... 36 0.095
9FAS2_YEAST (P19097) Fatty acid synthase alpha subunit (EC 2.3.1.... 33 0.80
10YKX1_SCHPO (O14116) Hypothetical protein C328.01c in chromosome I 31 4.0
11ILVB_SYNPX (Q7U5G1) Acetolactate synthase large subunit (EC 2.2.... 30 6.8

>IF3C_ORYSA (Q94HF1) Eukaryotic translation initiation factor 3 subunit 12|
           (eIF-3 p25) (eIF3k)
          Length = 226

 Score =  271 bits (694), Expect = 1e-72
 Identities = 131/143 (91%), Positives = 137/143 (95%)
 Frame = -1

Query: 681 LCLFLIPEHVQMEEQFKTMIVLSHYLETGRFRQFWDEASKSRNTLDAVPGFEQAIQSYAI 502
           LCLFLIPEHVQMEEQFKT+IVLSHYLET RFRQFWDEASK+RN LD VPGFEQAIQSYAI
Sbjct: 84  LCLFLIPEHVQMEEQFKTLIVLSHYLETARFRQFWDEASKNRNILDVVPGFEQAIQSYAI 143

Query: 501 HVLSLTYQKVPRPVLAEAINIEGLALDKFMEYHAANSGWVIENGTRSQLIVLPRNEFNHP 322
           HVLSLTYQKVPRPVLAEAINIEGLALDKF+E+H ANSGWVIE G RSQLIVLPRNEFNHP
Sbjct: 144 HVLSLTYQKVPRPVLAEAINIEGLALDKFLEHHIANSGWVIEKGARSQLIVLPRNEFNHP 203

Query: 321 ELKKNTADTVPFEHVTRIFPILS 253
           ELKKNTA+TVPFEHVTRIFP+LS
Sbjct: 204 ELKKNTAETVPFEHVTRIFPVLS 226



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>IF3C_ARATH (Q9SZA3) Eukaryotic translation initiation factor 3 subunit 12|
           (eIF-3 p25) (eIF3k)
          Length = 226

 Score =  217 bits (553), Expect = 2e-56
 Identities = 103/142 (72%), Positives = 123/142 (86%)
 Frame = -1

Query: 681 LCLFLIPEHVQMEEQFKTMIVLSHYLETGRFRQFWDEASKSRNTLDAVPGFEQAIQSYAI 502
           LCLFLIPE VQMEEQFK++IVLSHYLETGRF+QFWDEA+K+R+ L+AVPGFEQAIQ+YA 
Sbjct: 86  LCLFLIPERVQMEEQFKSLIVLSHYLETGRFQQFWDEAAKNRHILEAVPGFEQAIQAYAS 145

Query: 501 HVLSLTYQKVPRPVLAEAINIEGLALDKFMEYHAANSGWVIENGTRSQLIVLPRNEFNHP 322
           H+LSL+YQKVPR VLAEA+N++G +LDKF+E    NSGW++E    S  IVLP+NEFNHP
Sbjct: 146 HLLSLSYQKVPRSVLAEAVNMDGASLDKFIEQQVTNSGWIVEKEGGS--IVLPQNEFNHP 203

Query: 321 ELKKNTADTVPFEHVTRIFPIL 256
           ELKKNT + VP EH+ RIFPIL
Sbjct: 204 ELKKNTGENVPLEHIARIFPIL 225



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>IF3C_BOVIN (Q3T0V3) Eukaryotic translation initiation factor 3 subunit 12|
           (eIF-3 p25) (eIF3k)
          Length = 218

 Score = 64.3 bits (155), Expect = 3e-10
 Identities = 37/138 (26%), Positives = 68/138 (49%)
 Frame = -1

Query: 681 LCLFLIPEHVQMEEQFKTMIVLSHYLETGRFRQFWDEASKSRNTLDAVPGFEQAIQSYAI 502
           LC  +I +  Q E   + ++ L   LET  F+ FW    ++ + L+ + GFE +++ +  
Sbjct: 84  LCKCMIDQAHQEERPIRQILYLGDLLETCHFQAFWQALDENMDLLEGITGFEDSVRKFIC 143

Query: 501 HVLSLTYQKVPRPVLAEAINIEGLALDKFMEYHAANSGWVIENGTRSQLIVLPRNEFNHP 322
           HV+ +TYQ + R +LAE +   G   D  ++   +  GW  +     Q+ +  + E   P
Sbjct: 144 HVVGITYQHIDRWLLAEML---GDLTDSQLKVWMSKYGWSADES--GQIFICSQEESIKP 198

Query: 321 ELKKNTADTVPFEHVTRI 268
              KN  + + F+ V+ I
Sbjct: 199 ---KNIVEKIDFDSVSSI 213



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>IF3C_MOUSE (Q9DBZ5) Eukaryotic translation initiation factor 3 subunit 12|
           (eIF-3 p25) (eIF3k)
          Length = 218

 Score = 63.9 bits (154), Expect = 4e-10
 Identities = 37/138 (26%), Positives = 68/138 (49%)
 Frame = -1

Query: 681 LCLFLIPEHVQMEEQFKTMIVLSHYLETGRFRQFWDEASKSRNTLDAVPGFEQAIQSYAI 502
           LC  +I +  Q E   + ++ L   LET  F+ FW    ++ + L+ + GFE +++ +  
Sbjct: 84  LCKCMIDQAHQEERPIRQILYLGDLLETCHFQAFWQALDENMDLLEGITGFEDSVRKFIC 143

Query: 501 HVLSLTYQKVPRPVLAEAINIEGLALDKFMEYHAANSGWVIENGTRSQLIVLPRNEFNHP 322
           HV+ +TYQ + R +LAE +   G   D  ++   +  GW  +     Q+ +  + E   P
Sbjct: 144 HVVGITYQHIDRWLLAEML---GDLTDNQLKVWMSKYGWSADES--GQVFICSQEESIKP 198

Query: 321 ELKKNTADTVPFEHVTRI 268
              KN  + + F+ V+ I
Sbjct: 199 ---KNIVEKIDFDSVSSI 213



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>IF3C_HUMAN (Q9UBQ5) Eukaryotic translation initiation factor 3 subunit 12|
           (eIF-3 p25) (eIF-3 p28) (eIF3k) (Muscle-specific gene M9
           protein) (ARG134) (PLAC-24)
          Length = 218

 Score = 63.9 bits (154), Expect = 4e-10
 Identities = 37/138 (26%), Positives = 68/138 (49%)
 Frame = -1

Query: 681 LCLFLIPEHVQMEEQFKTMIVLSHYLETGRFRQFWDEASKSRNTLDAVPGFEQAIQSYAI 502
           LC  +I +  Q E   + ++ L   LET  F+ FW    ++ + L+ + GFE +++ +  
Sbjct: 84  LCKCMIDQAHQEERPIRQILYLGDLLETCHFQAFWQALDENMDLLEGITGFEDSVRKFIC 143

Query: 501 HVLSLTYQKVPRPVLAEAINIEGLALDKFMEYHAANSGWVIENGTRSQLIVLPRNEFNHP 322
           HV+ +TYQ + R +LAE +   G   D  ++   +  GW  +     Q+ +  + E   P
Sbjct: 144 HVVGITYQHIDRWLLAEML---GDLSDSQLKVWMSKYGWSADES--GQIFICSQEESIKP 198

Query: 321 ELKKNTADTVPFEHVTRI 268
              KN  + + F+ V+ I
Sbjct: 199 ---KNIVEKIDFDSVSSI 213



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>IF3C_DROME (Q9W2D9) Probable eukaryotic translation initiation factor 3|
           subunit 12 (eIF-3 p25) (eIF3k)
          Length = 222

 Score = 53.9 bits (128), Expect = 4e-07
 Identities = 38/136 (27%), Positives = 67/136 (49%), Gaps = 2/136 (1%)
 Frame = -1

Query: 678 CLFLIPEHVQMEEQFKTMIVLSHYLETGRFRQFWDEASKSRNTLDAVPGFEQAIQSYAIH 499
           CL L+P+ ++ +E  +T+I L+  LE   F  FW  A  +RN    + GF  +I+ +  H
Sbjct: 91  CL-LLPQQMK-DENVQTIIDLADILERADFTLFWQRAEVNRNMFRHITGFHDSIRKFVSH 148

Query: 498 VLSLTYQKVPRPVLAEAI-NIEGLALDKFMEYHAANSGWVIENGTRSQLIVLPRNEFNHP 322
           V+  T+Q + + +L E +  IE   L+           W+  NG ++Q   L        
Sbjct: 149 VVGTTFQTIRKDLLKELLGGIEDSTLE----------SWIKRNGWKNQGQGLVIVAMQDD 198

Query: 321 ELK-KNTADTVPFEHV 277
           ++K KN  + + F++V
Sbjct: 199 KIKTKNITEKIEFDNV 214



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>IF3C_CAEEL (Q9XUP3) Probable eukaryotic translation initiation factor 3|
           subunit 12 (eIF-3 p25) (eIF3k)
          Length = 240

 Score = 38.1 bits (87), Expect = 0.025
 Identities = 22/56 (39%), Positives = 34/56 (60%), Gaps = 1/56 (1%)
 Frame = -1

Query: 534 GFEQAIQSYAIHVLSLTYQKVPRPVLAEAINIEGLALDKFMEYHAANSGW-VIENG 370
           GFE A++ YA  V+S+T+QK+ + +L+  +   G A DK +   A + GW   ENG
Sbjct: 149 GFEDAVKHYACRVISVTFQKIEKKMLSRLL---GGASDKEVTALAQSFGWEAKENG 201



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>IF3C_CAEBR (Q61K76) Probable eukaryotic translation initiation factor 3|
           subunit 12 (eIF-3 p25) (eIF3k)
          Length = 240

 Score = 36.2 bits (82), Expect = 0.095
 Identities = 22/56 (39%), Positives = 32/56 (57%), Gaps = 1/56 (1%)
 Frame = -1

Query: 534 GFEQAIQSYAIHVLSLTYQKVPRPVLAEAINIEGLALDKFMEYHAANSGW-VIENG 370
           GFE AI+ YA  V+S+T+Q + + +L+  +   G A DK +   A   GW   ENG
Sbjct: 149 GFEDAIKHYACRVISVTFQNIEKKLLSSLL---GGASDKEVTALAKKFGWETKENG 201



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>FAS2_YEAST (P19097) Fatty acid synthase alpha subunit (EC 2.3.1.86) [Includes:|
           Acyl carrier; 3-oxoacyl-[acyl-carrier-protein] reductase
           (EC 1.1.1.100) (Beta-ketoacyl reductase);
           3-oxoacyl-[acyl-carrier-protein] synthase (EC 2.3.1.41)
           (Beta-ketoacyl synth
          Length = 1887

 Score = 33.1 bits (74), Expect = 0.80
 Identities = 19/49 (38%), Positives = 29/49 (59%), Gaps = 3/49 (6%)
 Frame = -1

Query: 528 EQAIQSYAIHVLSLTYQKVPRPVLAEAINIEGL---ALDKFMEYHAANS 391
           +Q++ S    ++    + V R V++EAINI      AL KFMEYH +N+
Sbjct: 410 KQSLLSLYFEIIHGVLKNVDREVVSEAINIMNRSNDALIKFMEYHISNT 458



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>YKX1_SCHPO (O14116) Hypothetical protein C328.01c in chromosome I|
          Length = 1234

 Score = 30.8 bits (68), Expect = 4.0
 Identities = 16/63 (25%), Positives = 31/63 (49%)
 Frame = -2

Query: 584  SFGTRHQRAVTHWMLYQVSSRQSKAMRFMCSP*HTRKFLGQFLQRPSTLRVLHWTSLWSI 405
            SFG    R +  +  Y ++S       F C P  +++FL  F    + L +  WTS+ ++
Sbjct: 865  SFG-HFLRRLREYCYYAIASFMRLGQAFFCVPGLSKQFLTAFFSHAAGLSLHQWTSMVNV 923

Query: 404  MLQ 396
            +++
Sbjct: 924  VIK 926



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>ILVB_SYNPX (Q7U5G1) Acetolactate synthase large subunit (EC 2.2.1.6) (AHAS)|
           (Acetohydroxy-acid synthase large subunit) (ALS)
          Length = 617

 Score = 30.0 bits (66), Expect = 6.8
 Identities = 29/118 (24%), Positives = 54/118 (45%), Gaps = 5/118 (4%)
 Frame = -1

Query: 633 KTMIVLSHYLETGRFRQ----FWDEASKSRNTLDAVPGFEQAIQSYAIHVLSLTYQKVPR 466
           K +IV +H+   G  RQ    F+DE   + + L+ +P F    +S+ +  + +T +++  
Sbjct: 484 KVVIVNNHW--QGMVRQWQESFYDERYSASDMLNGMPDFIALARSFGVDGVKITDRELLH 541

Query: 465 PVLAEAINIEGLALDKFMEYHAANSGWVIENG-TRSQLIVLPRNEFNHPELKKNTADT 295
             LA A+      +         N   ++  G + +Q++ LP    +HPEL   T  T
Sbjct: 542 RDLAAALQSPTPTMIDVHVRRGENCYPMVPPGKSNAQMVGLP----SHPELAMGTTRT 595


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 103,455,952
Number of Sequences: 219361
Number of extensions: 2234129
Number of successful extensions: 5126
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 4969
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5124
length of database: 80,573,946
effective HSP length: 108
effective length of database: 56,882,958
effective search space used: 6712189044
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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