ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd17m22
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1GSH1_ARATH (P46309) Glutamate--cysteine ligase, chloroplast prec... 163 5e-40
2GSH1_BRAJU (O23736) Glutamate--cysteine ligase, chloroplast prec... 158 2e-38
3GSH1_LYCES (O22493) Glutamate--cysteine ligase, chloroplast prec... 155 8e-38
4GSH1_MEDTR (Q9ZNX6) Glutamate--cysteine ligase, chloroplast prec... 153 4e-37
5NUDH_ZYMMO (Q9RH11) Probable (di)nucleoside polyphosphate hydrol... 34 0.48
6ACDS_MEGEL (Q06319) Acyl-CoA dehydrogenase, short-chain specific... 32 2.4
7TYCC_BREPA (O30409) Tyrocidine synthetase 3 (Tyrocidine syntheta... 31 3.1
8CCNB3_CANFA (Q659K0) G2/mitotic-specific cyclin-B3 31 4.1
9MKH1_SCHPO (Q10407) MAP kinase kinase kinase mkh1 (EC 2.7.11.25) 30 5.4
10RSSA_CAEEL (P46769) Probable 40S ribosomal protein SA (p40) 30 5.4
11TKT1_KLULA (Q12630) Transketolase (EC 2.2.1.1) (TK) 30 7.0
12IKAR_MOUSE (Q03267) DNA-binding protein Ikaros (Lymphoid transcr... 30 7.0
13U520_CAEEL (Q9U2G0) Putative U5 small nuclear ribonucleoprotein ... 30 9.1
14VG29_BPMU (Q9T1W5) Protein gp29 30 9.1

>GSH1_ARATH (P46309) Glutamate--cysteine ligase, chloroplast precursor (EC|
           6.3.2.2) (Gamma-glutamylcysteine synthetase) (Gamma-ECS)
           (GCS)
          Length = 522

 Score =  163 bits (412), Expect = 5e-40
 Identities = 78/102 (76%), Positives = 90/102 (88%)
 Frame = -3

Query: 680 SLQSIIDMTSDWTKEEREMLRRKVPVTGLKTPFRDGYVRDLAEDVLQLAKNGLERRGYKE 501
           SLQ+I+D+T+DWT  EREMLR KVPVTGLKTPFRDG ++ +AEDVL+LAK+GLERRGYKE
Sbjct: 421 SLQAILDLTADWTPAEREMLRNKVPVTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKE 480

Query: 500 VGFLREVDEVVRTGVTPAEKLLNLYETKWQRSVDPVFEELLY 375
            GFL  VDEVVRTGVTPAEKLL +Y  +W +SVDPVFEELLY
Sbjct: 481 AGFLNAVDEVVRTGVTPAEKLLEMYNGEWGQSVDPVFEELLY 522



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>GSH1_BRAJU (O23736) Glutamate--cysteine ligase, chloroplast precursor (EC|
           6.3.2.2) (Gamma-glutamylcysteine synthetase) (Gamma-ECS)
           (GCS)
          Length = 514

 Score =  158 bits (399), Expect = 2e-38
 Identities = 75/101 (74%), Positives = 88/101 (87%)
 Frame = -3

Query: 677 LQSIIDMTSDWTKEEREMLRRKVPVTGLKTPFRDGYVRDLAEDVLQLAKNGLERRGYKEV 498
           LQS++D+T+DWT  EREMLR KVPVTGLKTPFRDG ++ +AEDVL+LAK+GLERRGYKEV
Sbjct: 414 LQSVLDLTADWTPAEREMLRNKVPVTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEV 473

Query: 497 GFLREVDEVVRTGVTPAEKLLNLYETKWQRSVDPVFEELLY 375
           GFL  V EVVRTGVTPAE LL +Y  +W +SVDPVF+ELLY
Sbjct: 474 GFLNAVTEVVRTGVTPAENLLEMYNGEWGQSVDPVFQELLY 514



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>GSH1_LYCES (O22493) Glutamate--cysteine ligase, chloroplast precursor (EC|
           6.3.2.2) (Gamma-glutamylcysteine synthetase) (Gamma-ECS)
           (GCS)
          Length = 523

 Score =  155 bits (393), Expect = 8e-38
 Identities = 74/102 (72%), Positives = 87/102 (85%)
 Frame = -3

Query: 680 SLQSIIDMTSDWTKEEREMLRRKVPVTGLKTPFRDGYVRDLAEDVLQLAKNGLERRGYKE 501
           SLQS++DMT DWT EER+MLR KVP +GLKTPFRDG +  +A+DV++LAK GLERRG+KE
Sbjct: 422 SLQSVLDMTFDWTAEERDMLRNKVPKSGLKTPFRDGLLMHVAQDVVKLAKEGLERRGFKE 481

Query: 500 VGFLREVDEVVRTGVTPAEKLLNLYETKWQRSVDPVFEELLY 375
            GFL EV EVV+TGVTPAEKLL LY  KW +SVDP+FEELLY
Sbjct: 482 TGFLNEVAEVVKTGVTPAEKLLELYHGKWGQSVDPIFEELLY 523



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>GSH1_MEDTR (Q9ZNX6) Glutamate--cysteine ligase, chloroplast precursor (EC|
           6.3.2.2) (Gamma-glutamylcysteine synthetase) (Gamma-ECS)
           (GCS)
          Length = 508

 Score =  153 bits (387), Expect = 4e-37
 Identities = 74/102 (72%), Positives = 88/102 (86%)
 Frame = -3

Query: 680 SLQSIIDMTSDWTKEEREMLRRKVPVTGLKTPFRDGYVRDLAEDVLQLAKNGLERRGYKE 501
           SLQ ++DMT+DWT EEREMLR KV VTGLKTPFRDG ++ +AE+VL+LAK+GLERRG+KE
Sbjct: 407 SLQRVLDMTADWTLEEREMLRNKVTVTGLKTPFRDGLLKHVAEEVLELAKDGLERRGFKE 466

Query: 500 VGFLREVDEVVRTGVTPAEKLLNLYETKWQRSVDPVFEELLY 375
            GFL  V EVVRTGVTPAE+LL LY  KW++SVD VF+ELLY
Sbjct: 467 SGFLNAVAEVVRTGVTPAERLLELYHGKWEQSVDHVFDELLY 508



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>NUDH_ZYMMO (Q9RH11) Probable (di)nucleoside polyphosphate hydrolase (EC|
           3.6.1.-)
          Length = 155

 Score = 33.9 bits (76), Expect = 0.48
 Identities = 17/50 (34%), Positives = 28/50 (56%)
 Frame = -3

Query: 563 DLAEDVLQLAKNGLERRGYKEVGFLREVDEVVRTGVTPAEKLLNLYETKW 414
           D+ E+  Q+ + GLE +   EVG LRE++E   TG+ P    +  +  +W
Sbjct: 28  DMKEEAWQMPQGGLEAKETPEVGVLRELEE--ETGIPPRMVAIISHTKEW 75



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>ACDS_MEGEL (Q06319) Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)|
           (SCAD) (Butyryl-CoA dehydrogenase) (BCAD)
          Length = 383

 Score = 31.6 bits (70), Expect = 2.4
 Identities = 30/117 (25%), Positives = 50/117 (42%), Gaps = 8/117 (6%)
 Frame = -3

Query: 578 DGYVRDLAEDVLQLAKNGLER--------RGYKEVGFLREVDEVVRTGVTPAEKLLNLYE 423
           D  + D+ +D L+LA +  E+        R +K +     +DE++  G+T A      +E
Sbjct: 2   DFNLTDIQQDFLKLAHDFGEKKLAPTVTERDHKGIYDKELIDELLSLGITGA-----YFE 56

Query: 422 TKWQRSVDPVFEELLY*YTESHITSVDAGQGGQGLQTVSLYKNPVLNSTSHLTRETY 252
            K+  S D   + L Y      +   DAG       TVSL  NP+    +   +E +
Sbjct: 57  EKYGGSGDDGGDVLSYILAVEELAKYDAGVAITLSATVSLCANPIWQFGTEAQKEKF 113



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>TYCC_BREPA (O30409) Tyrocidine synthetase 3 (Tyrocidine synthetase III)|
            [Includes: ATP-dependent asparagine adenylase (AsnA)
            (Asparagine activase); ATP-dependent glutamine adenylase
            (GlnA) (Glutamine activase); ATP-dependent tyrosine
            adenylase (TyrA) (Ty
          Length = 6486

 Score = 31.2 bits (69), Expect = 3.1
 Identities = 21/64 (32%), Positives = 35/64 (54%), Gaps = 4/64 (6%)
 Frame = +3

Query: 177  TGERKTASH---KQSVPSSKLFAAARPEVGFPRQVACGIEDGIF-VQGYSLKTLSTLSSV 344
            +GER T S     Q+   +KL    +  +G P   A GI D  F + G+SLK ++ ++ V
Sbjct: 4064 SGERTTGSAFVAAQNDTEAKLQQIWQEVLGIP---AIGIHDNFFEIGGHSLKAMNVITQV 4120

Query: 345  HRSY 356
            H+++
Sbjct: 4121 HKTF 4124



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>CCNB3_CANFA (Q659K0) G2/mitotic-specific cyclin-B3|
          Length = 1330

 Score = 30.8 bits (68), Expect = 4.1
 Identities = 13/29 (44%), Positives = 14/29 (48%)
 Frame = +1

Query: 289  TGFLYRDTV*RPCPPCPASTEVICDSVYQ 375
            T FL       PCPPC      ICD +YQ
Sbjct: 1152 TAFLIAAKFEEPCPPCVDDFLYICDDIYQ 1180



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>MKH1_SCHPO (Q10407) MAP kinase kinase kinase mkh1 (EC 2.7.11.25)|
          Length = 1116

 Score = 30.4 bits (67), Expect = 5.4
 Identities = 24/86 (27%), Positives = 42/86 (48%), Gaps = 4/86 (4%)
 Frame = +1

Query: 4   HSELFSGNEVPHHTTEMDL----FRIQAVHQGKVKKGSVQTGVCSGSLEMREKS*VVKDP 171
           ++EL S  +  H TT ++L      + A       KG+V + +   S  + +K+ + K P
Sbjct: 167 NNELLSTGKNSHQTTSLNLEGSPINLHAY------KGTVTSIINDDSRNINKKT-LSKQP 219

Query: 172 PQLEKEKQQATNRVFRAQNYSQPRDR 249
               KEKQ +  R FR   +S+ +D+
Sbjct: 220 VSEHKEKQTSFLRRFRVPGFSRDKDK 245



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>RSSA_CAEEL (P46769) Probable 40S ribosomal protein SA (p40)|
          Length = 275

 Score = 30.4 bits (67), Expect = 5.4
 Identities = 19/55 (34%), Positives = 26/55 (47%)
 Frame = +1

Query: 82  QGKVKKGSVQTGVCSGSLEMREKS*VVKDPPQLEKEKQQATNRVFRAQNYSQPRD 246
           +GK+ +   QTG      E+       +DP + EKE+  A   V  AQ Y QP D
Sbjct: 185 RGKISR---QTGFVLEGKEIMPDLYFYRDPTETEKEETGAHADVAEAQEYQQPTD 236



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>TKT1_KLULA (Q12630) Transketolase (EC 2.2.1.1) (TK)|
          Length = 679

 Score = 30.0 bits (66), Expect = 7.0
 Identities = 18/54 (33%), Positives = 30/54 (55%)
 Frame = -2

Query: 246 VSRLRIILSSEHSVCGLLFFFLQLWGIFHNLRFFSHFQGAGTNPCLYRPLFHLP 85
           ++R R +LS+ H+ C LL+  L L+G   ++    HF+  G+    + P F LP
Sbjct: 58  INRDRFVLSNGHA-CALLYSLLHLFGYDMSIEDLKHFRHLGSKTPGH-PEFELP 109



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>IKAR_MOUSE (Q03267) DNA-binding protein Ikaros (Lymphoid transcription factor|
           LyF-1)
          Length = 517

 Score = 30.0 bits (66), Expect = 7.0
 Identities = 12/22 (54%), Positives = 15/22 (68%)
 Frame = -2

Query: 324 GSSDCIPVQKSRPQFHKPPDEG 259
           GSS+ +PV  S  Q HKPP +G
Sbjct: 332 GSSEVVPVISSMYQLHKPPSDG 353



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>U520_CAEEL (Q9U2G0) Putative U5 small nuclear ribonucleoprotein 200 kDa helicase|
            (EC 3.6.1.-)
          Length = 2145

 Score = 29.6 bits (65), Expect = 9.1
 Identities = 19/80 (23%), Positives = 31/80 (38%), Gaps = 3/80 (3%)
 Frame = -3

Query: 554  EDVLQLAKNGLERRGYKEVGFLREVD---EVVRTGVTPAEKLLNLYETKWQRSVDPVFEE 384
            E+V+  A NG  +    E+  LR  +   E     +TP E +       W+R ++P    
Sbjct: 1338 ENVIVCAPNGSGKTAIAELAVLRHFENTPEAKAVYITPMEDMATKVYADWKRRLEPAIGH 1397

Query: 383  LLY*YTESHITSVDAGQGGQ 324
             +   T      +   Q GQ
Sbjct: 1398 TIVLLTGEQTMDLKLAQRGQ 1417



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>VG29_BPMU (Q9T1W5) Protein gp29|
          Length = 512

 Score = 29.6 bits (65), Expect = 9.1
 Identities = 14/52 (26%), Positives = 27/52 (51%)
 Frame = -3

Query: 530 NGLERRGYKEVGFLREVDEVVRTGVTPAEKLLNLYETKWQRSVDPVFEELLY 375
           NG ++        + + D++ R GV+P +         WQRSVDP+ + +++
Sbjct: 421 NGSQKEAALSAEDIPQEDDIDRMGVSPED---------WQRSVDPLLKPVIF 463


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 104,971,379
Number of Sequences: 219361
Number of extensions: 2347914
Number of successful extensions: 7179
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 6900
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7176
length of database: 80,573,946
effective HSP length: 109
effective length of database: 56,663,597
effective search space used: 6912958834
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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