ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd17j04
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1SGAT_ARATH (Q56YA5) Serine--glyoxylate aminotransferase (EC 2.6.... 229 4e-60
2SGAA_METEX (P55819) Serine--glyoxylate aminotransferase (EC 2.6.... 158 2e-38
3SGAA_HYPME (O08374) Serine--glyoxylate aminotransferase (EC 2.6.... 153 5e-37
4Y959_METJA (Q58369) Putative aminotransferase MJ0959 (EC 2.6.1.-) 89 1e-17
5DHSS_ANACY (P16421) Soluble hydrogenase 42 kDa subunit (EC 1.12.... 79 9e-15
6DHSS_SYNP1 (P14776) Soluble hydrogenase, small subunit (EC 1.12.... 73 8e-13
7PUCG_BACSU (O32148) Purine catabolism protein pucG (EC 2.-.-.-) 45 1e-04
8SPYA_RAT (P09139) Serine--pyruvate aminotransferase, mitochondri... 45 2e-04
9SPYA_CALJA (P31029) Serine--pyruvate aminotransferase, mitochond... 44 3e-04
10SPYA_HUMAN (P21549) Serine--pyruvate aminotransferase (EC 2.6.1.... 42 0.002
11SPYA_FELCA (P41689) Serine--pyruvate aminotransferase, mitochond... 41 0.003
12SGAT_MAIZE (P84187) Serine--glyoxylate aminotransferase (EC 2.6.... 40 0.006
13SPYA_RABIT (P31030) Serine--pyruvate aminotransferase (EC 2.6.1.... 37 0.039
14SPYA_MOUSE (O35423) Serine--pyruvate aminotransferase, mitochond... 36 0.11
15LAS17_YEAST (Q12446) Proline-rich protein LAS17 33 0.95
16CEFD_NOCLA (Q03046) Isopenicillin N epimerase (EC 5.1.1.17) 33 0.95
17POLN_RUBVT (P13889) Nonstructural polyprotein [Contains: Proteas... 32 2.1
18F100A_RAT (Q6AXN0) Protein FAM100A 32 2.1
19YGHE_ECOLI (Q46833) Putative general secretion pathway protein L... 30 8.0

>SGAT_ARATH (Q56YA5) Serine--glyoxylate aminotransferase (EC 2.6.1.45)|
           (Alanine--glyoxylate aminotransferase) (EC 2.6.1.44)
           (AGT) (Serine--pyruvate aminotransferase) (EC 2.6.1.51)
          Length = 401

 Score =  229 bits (585), Expect = 4e-60
 Identities = 112/153 (73%), Positives = 128/153 (83%)
 Frame = -3

Query: 645 YTPSIQXXXXXXXXXXXLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTV 466
           YTPSIQ           +FEEGL+N+I RH RLG ATRLAV AWGLKNCT+KEE  S+TV
Sbjct: 249 YTPSIQLLYGLRAALDLIFEEGLENIIARHARLGKATRLAVEAWGLKNCTQKEEWISNTV 308

Query: 465 TAVVVPPYIDSSEIVKHAWKRYNLSLGLGLNKVAGKVFRIGHLGHLNELQLLGCLGGVEM 286
           TAV+VPP+ID SEIV+ AW+RYNLSLGLGLNKVAGKVFRIGHLG++NELQLLGCL GVEM
Sbjct: 309 TAVMVPPHIDGSEIVRRAWQRYNLSLGLGLNKVAGKVFRIGHLGNVNELQLLGCLAGVEM 368

Query: 285 VLKDIGYPVKLGSGVAAAAAYLSNSTPLIPSRI 187
           +LKD+GYPV +GSGVAAA+ YL +  PLIPSRI
Sbjct: 369 ILKDVGYPVVMGSGVAAASTYLQHHIPLIPSRI 401



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>SGAA_METEX (P55819) Serine--glyoxylate aminotransferase (EC 2.6.1.45) (SGAT)|
          Length = 379

 Score =  158 bits (399), Expect = 2e-38
 Identities = 82/142 (57%), Positives = 100/142 (70%)
 Frame = -3

Query: 645 YTPSIQXXXXXXXXXXXLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTV 466
           YTP +            LFEEGL+NV  RH  LG ATR AVAAWGLK C +  E  SDTV
Sbjct: 230 YTPPLPLLYGLREALACLFEEGLENVYHRHAVLGEATRQAVAAWGLKTCAKSPEWNSDTV 289

Query: 465 TAVVVPPYIDSSEIVKHAWKRYNLSLGLGLNKVAGKVFRIGHLGHLNELQLLGCLGGVEM 286
           TA++ P  +D+++I+KHA+ RYNL+LG GL++VAGKVFRIGH+G LNEL LLG + G EM
Sbjct: 290 TAILAPEGVDAAKIIKHAYVRYNLALGAGLSQVAGKVFRIGHVGDLNELSLLGAIAGAEM 349

Query: 285 VLKDIGYPVKLGSGVAAAAAYL 220
            L D G  V  GSGVAAA++YL
Sbjct: 350 SLIDNGVKVTPGSGVAAASSYL 371



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>SGAA_HYPME (O08374) Serine--glyoxylate aminotransferase (EC 2.6.1.45) (SGAT)|
          Length = 404

 Score =  153 bits (386), Expect = 5e-37
 Identities = 81/145 (55%), Positives = 97/145 (66%)
 Frame = -3

Query: 645 YTPSIQXXXXXXXXXXXLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTV 466
           YTP+ Q           LF EGLDNV  RHTRL +  R AV AWGLK C ++ + +SDTV
Sbjct: 244 YTPATQLLRGLRTSLDLLFAEGLDNVFARHTRLASGVRAAVDAWGLKLCAKEPKWYSDTV 303

Query: 465 TAVVVPPYIDSSEIVKHAWKRYNLSLGLGLNKVAGKVFRIGHLGHLNELQLLGCLGGVEM 286
           +A++VP  IDS+ I K A+ RYN S GLGLNKVAGKVFRIGHLG L+E+ + G L   EM
Sbjct: 304 SAILVPEGIDSNAITKTAYYRYNTSFGLGLNKVAGKVFRIGHLGMLDEVMIGGALFAAEM 363

Query: 285 VLKDIGYPVKLGSGVAAAAAYLSNS 211
            LKD G  +KLGSG  AAA Y S +
Sbjct: 364 ALKDNGVNLKLGSGTGAAAEYFSKN 388



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>Y959_METJA (Q58369) Putative aminotransferase MJ0959 (EC 2.6.1.-)|
          Length = 385

 Score = 89.0 bits (219), Expect = 1e-17
 Identities = 54/142 (38%), Positives = 77/142 (54%)
 Frame = -3

Query: 645 YTPSIQXXXXXXXXXXXLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTV 466
           YTPS+            + EEG++N +KRH RL  ATR  + A G++    KE   S TV
Sbjct: 243 YTPSVNLTYALNVALDLVLEEGIENRVKRHERLAKATRAGLEAMGIE-LFAKERARSVTV 301

Query: 465 TAVVVPPYIDSSEIVKHAWKRYNLSLGLGLNKVAGKVFRIGHLGHLNELQLLGCLGGVEM 286
           T+   P  I+ S+       +YN+ +  G   +AGK+FRIGH+G   E ++L  L  VE+
Sbjct: 302 TSAKYPEGIEDSKFRGILSNKYNIVVAGGQKHLAGKIFRIGHMGICGEKEVLATLACVEL 361

Query: 285 VLKDIGYPVKLGSGVAAAAAYL 220
            LK++G+ VK  SGV  A   L
Sbjct: 362 ALKELGFEVK-ESGVEVAKEVL 382



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>DHSS_ANACY (P16421) Soluble hydrogenase 42 kDa subunit (EC 1.12.-.-) (Tritium|
           exchange subunit)
          Length = 383

 Score = 79.3 bits (194), Expect = 9e-15
 Identities = 47/145 (32%), Positives = 81/145 (55%), Gaps = 1/145 (0%)
 Frame = -3

Query: 645 YTPSIQXXXXXXXXXXXLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTV 466
           +TP +            + +EGL+++  RH R   ATR A+ A  L      +E  S  +
Sbjct: 241 FTPPVNLMVALHTTLGMMKKEGLESIFTRHERQKNATRAAMKALNLP-LFAADECASPAI 299

Query: 465 TAVVVPPYIDSSEIVKHAWKRYNLSLGLGLNKVAGKVFRIGHLGHLNELQLLGCLGGVEM 286
           TAV  P  +++ +I     KR++++L  G + ++ K+FR+GHLG +++  +L C+  +E+
Sbjct: 300 TAVATPG-MEADKIRSLMKKRFDIALAGGQDHLSNKIFRVGHLGFVSDRDILSCIASLEV 358

Query: 285 VLKDIGYP-VKLGSGVAAAAAYLSN 214
           VL ++G+     G+GVAAAA   SN
Sbjct: 359 VLLELGHENFNSGAGVAAAARVFSN 383



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>DHSS_SYNP1 (P14776) Soluble hydrogenase, small subunit (EC 1.12.-.-) (Tritium|
           exchange subunit)
          Length = 384

 Score = 72.8 bits (177), Expect = 8e-13
 Identities = 47/146 (32%), Positives = 77/146 (52%), Gaps = 1/146 (0%)
 Frame = -3

Query: 645 YTPSIQXXXXXXXXXXXLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTV 466
           +TP +            +  EGL+ + +RH RL  ATR A+ A  L      +   S  +
Sbjct: 241 FTPPVNLFFALKTALEMMQAEGLEAIFQRHQRLMQATRAAMKALNLP-LYAADSCASPAI 299

Query: 465 TAVVVPPYIDSSEIVKHAWKRYNLSLGLGLNKVAGKVFRIGHLGHLNELQLLGCLGGVEM 286
           TAV  P  +++  I     KR++++L  G + + G++FRIGHLG + +  +L  +  +E 
Sbjct: 300 TAVA-PQGVEAENIRSLMKKRFDIALAGGQDHLKGQIFRIGHLGFVGDRDILAAVSALEA 358

Query: 285 VLKDIGYP-VKLGSGVAAAAAYLSNS 211
           VL ++GY     G+GVAAA+  LS +
Sbjct: 359 VLAELGYTNFTPGAGVAAASRVLSTA 384



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>PUCG_BACSU (O32148) Purine catabolism protein pucG (EC 2.-.-.-)|
          Length = 416

 Score = 45.4 bits (106), Expect = 1e-04
 Identities = 33/127 (25%), Positives = 56/127 (44%), Gaps = 1/127 (0%)
 Frame = -3

Query: 588 EEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTVTAVVVPPYIDSSEIVKHAW 409
           EEGL+   +RH     A    + A GL+   +        VT V +P  ID   +     
Sbjct: 285 EEGLETRFERHRHHEAALAAGIKAMGLRLFGDDSCKMP-VVTCVEIPGGIDGESVRDMLL 343

Query: 408 KRYNLSLGLGLNKVAGKVFRIGHLGH-LNELQLLGCLGGVEMVLKDIGYPVKLGSGVAAA 232
            ++ + +      +AGK++RIG +G+   +  +L  L G+E VL      ++ G  + AA
Sbjct: 344 AQFGIEIASSFGPLAGKIWRIGTMGYSCRKENVLFVLAGLEAVLLRHNAGIEAGKALQAA 403

Query: 231 AAYLSNS 211
                N+
Sbjct: 404 LDVYENA 410



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>SPYA_RAT (P09139) Serine--pyruvate aminotransferase, mitochondrial precursor|
           (EC 2.6.1.51) (SPT) (Alanine--glyoxylate
           aminotransferase) (EC 2.6.1.44) (AGT)
          Length = 414

 Score = 44.7 bits (104), Expect = 2e-04
 Identities = 27/86 (31%), Positives = 40/86 (46%)
 Frame = -3

Query: 588 EEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTVTAVVVPPYIDSSEIVKHAW 409
           E+GL+N  +RH          +   GLK   +  E    T+T V VP   +  +IV +  
Sbjct: 303 EQGLENSWRRHREATAHLHKCLRELGLKFFVKDPEIRLPTITTVTVPAGYNWRDIVSYVL 362

Query: 408 KRYNLSLGLGLNKVAGKVFRIGHLGH 331
             +N+ +  GL     KV RIG LG+
Sbjct: 363 DHFNIEISGGLGPSEDKVLRIGLLGY 388



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>SPYA_CALJA (P31029) Serine--pyruvate aminotransferase, mitochondrial precursor|
           (EC 2.6.1.51) (SPT) (Alanine--glyoxylate
           aminotransferase) (EC 2.6.1.44) (AGT)
          Length = 414

 Score = 44.3 bits (103), Expect = 3e-04
 Identities = 27/85 (31%), Positives = 41/85 (48%)
 Frame = -3

Query: 588 EEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTVTAVVVPPYIDSSEIVKHAW 409
           E+GL+N  ++H          + A GL+   +       TVT V VP   D  +IV +  
Sbjct: 303 EQGLENSWRKHREAAAYLHGRLQALGLRLFVKDPALRLPTVTTVAVPTGYDWRDIVSYLI 362

Query: 408 KRYNLSLGLGLNKVAGKVFRIGHLG 334
           +R+ + +  GL    GKV RIG +G
Sbjct: 363 ERFGIEITGGLGPSTGKVLRIGLMG 387



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>SPYA_HUMAN (P21549) Serine--pyruvate aminotransferase (EC 2.6.1.51) (SPT)|
           (Alanine--glyoxylate aminotransferase) (EC 2.6.1.44)
           (AGT)
          Length = 392

 Score = 42.0 bits (97), Expect = 0.002
 Identities = 27/85 (31%), Positives = 40/85 (47%)
 Frame = -3

Query: 588 EEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTVTAVVVPPYIDSSEIVKHAW 409
           E+GL+N  ++H          + A GL+   +       TVT V VP   D  +IV +  
Sbjct: 281 EQGLENSWRQHREAAAYLHGRLQALGLQLFVKDPALRLPTVTTVAVPAGYDWRDIVSYVI 340

Query: 408 KRYNLSLGLGLNKVAGKVFRIGHLG 334
             +++ +  GL    GKV RIG LG
Sbjct: 341 DHFDIEIMGGLGPSTGKVLRIGLLG 365



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>SPYA_FELCA (P41689) Serine--pyruvate aminotransferase, mitochondrial precursor|
           (EC 2.6.1.51) (SPT) (Alanine--glyoxylate
           aminotransferase) (EC 2.6.1.44) (AGT)
          Length = 414

 Score = 41.2 bits (95), Expect = 0.003
 Identities = 26/85 (30%), Positives = 40/85 (47%)
 Frame = -3

Query: 588 EEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTVTAVVVPPYIDSSEIVKHAW 409
           E+GL+N  ++H  +       +   GL+   +       TVT V VP   D  +IV +  
Sbjct: 303 EQGLENSWRQHREVTAYLHGRLQGLGLQLFVKDPALRLPTVTTVAVPAGYDWRDIVNYVM 362

Query: 408 KRYNLSLGLGLNKVAGKVFRIGHLG 334
             +++ +  GL    GKV RIG LG
Sbjct: 363 DHFDIEITGGLGPSMGKVLRIGLLG 387



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>SGAT_MAIZE (P84187) Serine--glyoxylate aminotransferase (EC 2.6.1.45) (SGAT)|
           (Alanine--glyoxylate aminotransferase) (EC 2.6.1.44)
           (AGT) (Fragments)
          Length = 136

 Score = 40.0 bits (92), Expect = 0.006
 Identities = 28/62 (45%), Positives = 30/62 (48%), Gaps = 4/62 (6%)
 Frame = -3

Query: 429 EIVKHAW---KRYNLSLGLGLNKVA-GKVFRIGHLGHLNELQLLGCLGGVEMVLKDIGYP 262
           E+   AW    RYNLSLGLGLNKVA GKVFR                        D+GYP
Sbjct: 99  ELAVEAWGLSNRYNLSLGLGLNKVAGGKVFR------------------------DVGYP 134

Query: 261 VK 256
           VK
Sbjct: 135 VK 136



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>SPYA_RABIT (P31030) Serine--pyruvate aminotransferase (EC 2.6.1.51) (SPT)|
           (Alanine--glyoxylate aminotransferase) (EC 2.6.1.44)
           (AGT)
          Length = 392

 Score = 37.4 bits (85), Expect = 0.039
 Identities = 25/85 (29%), Positives = 38/85 (44%)
 Frame = -3

Query: 588 EEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTVTAVVVPPYIDSSEIVKHAW 409
           E+GL+   +RH  +       +   GL+   +       TVT V+VP      +IV +  
Sbjct: 281 EQGLEKSWQRHREVAQHLYRRLQELGLQLFVKDPALRLPTVTTVIVPASYRWRDIVSYVM 340

Query: 408 KRYNLSLGLGLNKVAGKVFRIGHLG 334
             + + +  GL   A KV RIG LG
Sbjct: 341 HHFGIEITGGLGPSADKVLRIGLLG 365



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>SPYA_MOUSE (O35423) Serine--pyruvate aminotransferase, mitochondrial precursor|
           (EC 2.6.1.51) (SPT) (Alanine--glyoxylate
           aminotransferase) (EC 2.6.1.44) (AGT)
          Length = 413

 Score = 35.8 bits (81), Expect = 0.11
 Identities = 24/86 (27%), Positives = 38/86 (44%)
 Frame = -3

Query: 588 EEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTVTAVVVPPYIDSSEIVKHAW 409
           E GL+N  +RH          +   GLK   +  E    T+T V    Y +  +IV +  
Sbjct: 303 ERGLENCWRRHREATAHLHKHLQEMGLKFFVKDPEIRLPTITTVTAAGY-NWRDIVSYVL 361

Query: 408 KRYNLSLGLGLNKVAGKVFRIGHLGH 331
             +++ +  GL     +V RIG LG+
Sbjct: 362 DHFSIEISGGLGPTEERVLRIGLLGY 387



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>LAS17_YEAST (Q12446) Proline-rich protein LAS17|
          Length = 633

 Score = 32.7 bits (73), Expect = 0.95
 Identities = 16/32 (50%), Positives = 17/32 (53%), Gaps = 1/32 (3%)
 Frame = +3

Query: 213 NSRGTPPRPPRRCQAS-PGTRCP*APSPPRRG 305
           N+R  PP PP R      G R P  P PPRRG
Sbjct: 320 NNRPVPPPPPMRTTTEGSGVRLPAPPPPPRRG 351



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>CEFD_NOCLA (Q03046) Isopenicillin N epimerase (EC 5.1.1.17)|
          Length = 398

 Score = 32.7 bits (73), Expect = 0.95
 Identities = 23/85 (27%), Positives = 42/85 (49%), Gaps = 3/85 (3%)
 Frame = -3

Query: 591 FEEGLDN--VIKRHTRLGTATRLAVAAW-GLKNCTEKEENFSDTVTAVVVPPYIDSSEIV 421
           F+ GL +  V +R   L   TR  ++ W GL+  T      S  +TA  +PP  D++ + 
Sbjct: 292 FQAGLGHGRVRERMRELAAFTRERLSGWRGLEPVTPAHPGLSGAMTAFRLPPGTDTAGLR 351

Query: 420 KHAWKRYNLSLGLGLNKVAGKVFRI 346
              W R+ + + + L++  G + R+
Sbjct: 352 HGLWDRFRIDVPV-LDRPDGPLLRV 375



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>POLN_RUBVT (P13889) Nonstructural polyprotein [Contains: Protease (EC|
           3.4.22.-) (p150); RNA-directed RNA polymerase/helicase
           (EC 2.7.7.48) (EC 3.6.1.-) (p90)]
          Length = 2115

 Score = 31.6 bits (70), Expect = 2.1
 Identities = 15/33 (45%), Positives = 19/33 (57%)
 Frame = +3

Query: 213 NSRGTPPRPPRRCQASPGTRCP*APSPPRRGNP 311
           ++RGTPP  P R    P    P  P+PPR G+P
Sbjct: 740 DARGTPPPAPAR---DPPPPAPSPPAPPRAGDP 769



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>F100A_RAT (Q6AXN0) Protein FAM100A|
          Length = 176

 Score = 31.6 bits (70), Expect = 2.1
 Identities = 15/44 (34%), Positives = 20/44 (45%)
 Frame = +2

Query: 215 FERYAAAAATPLPSFTGYPMSLSTISTPPRQPRSCSSLRWPRWP 346
           F R  A+ +      +G PM+ S  S PP  P +  S   P WP
Sbjct: 81  FSRLKASESFHSGGSSGSPMATSATSPPPHFPHATGSFATPSWP 124



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>YGHE_ECOLI (Q46833) Putative general secretion pathway protein L-type yghE|
          Length = 286

 Score = 29.6 bits (65), Expect = 8.0
 Identities = 17/53 (32%), Positives = 22/53 (41%)
 Frame = +2

Query: 191 LEGMRGVEFERYAAAAATPLPSFTGYPMSLSTISTPPRQPRSCSSLRWPRWPI 349
           L    G + ER    A   LP+ T Y      + T P QPR     +W RW +
Sbjct: 87  LSAWAGADAERLTVTA---LPAITTYGEPEGNLLTGPWQPRVSYRKQWARWRV 136


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,684,129
Number of Sequences: 219361
Number of extensions: 1490853
Number of successful extensions: 6055
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 5384
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6028
length of database: 80,573,946
effective HSP length: 108
effective length of database: 56,882,958
effective search space used: 6086476506
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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