ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd17h22
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1CIPK1_ORYSA (Q6X4A2) CIPK-like protein 1 (EC 2.7.11.1) (OsCK1) 155 5e-38
2CPK24_ARATH (Q9LDI3) CBL-interacting serine/threonine-protein ki... 139 4e-33
3CIPK1_ARATH (Q8RWC9) CBL-interacting serine/threonine-protein ki... 96 5e-20
4CPK15_ARATH (P92937) CBL-interacting serine/threonine-protein ki... 68 1e-11
5CPK11_ARATH (O22932) CBL-interacting serine/threonine-protein ki... 45 1e-04
6CHK1_MOUSE (O35280) Serine/threonine-protein kinase Chk1 (EC 2.7... 37 0.028
7CHK1_HUMAN (O14757) Serine/threonine-protein kinase Chk1 (EC 2.7... 37 0.036
8CHK1_RAT (Q91ZN7) Serine/threonine-protein kinase Chk1 (EC 2.7.1... 36 0.062
9CHK1_CHICK (Q8AYC9) Serine/threonine-protein kinase Chk1 (EC 2.7... 35 0.11
10CHK1_XENLA (Q6DE87) Serine/threonine-protein kinase Chk1 (EC 2.7... 32 0.89
11SLH1_YEAST (P53327) Antiviral helicase SLH1 (EC 3.6.1.-) (SKI2-l... 32 1.2
12CHK1_SCHPO (P34208) Serine/threonine-protein kinase chk1 (EC 2.7... 32 1.5
13TRM1_PYRAB (Q9V1P3) N(2),N(2)-dimethylguanosine tRNA methyltrans... 29 9.9
14PPCK_CHLCV (Q821M4) Phosphoenolpyruvate carboxykinase [GTP] (EC ... 29 9.9
15Y861_METJA (Q58271) Hypothetical UPF0036 protein MJ0861 29 9.9
16ILVH_ECOLI (P00894) Acetolactate synthase isozyme III small subu... 29 9.9

>CIPK1_ORYSA (Q6X4A2) CIPK-like protein 1 (EC 2.7.11.1) (OsCK1)|
          Length = 449

 Score =  155 bits (393), Expect = 5e-38
 Identities = 74/131 (56%), Positives = 101/131 (77%)
 Frame = -3

Query: 543 ERPAVMNAFELISTSQGLNLGTLFEKQTDSVKRETRFASRLPANEILSKIEAAAGPMGFN 364
           ++P  MNAFELIS +Q LNL  LFE + +  KRETRF S+ P  EI++KIE AA P+GF+
Sbjct: 313 DQPTSMNAFELISLNQALNLDNLFEAKKE-YKRETRFTSQCPPKEIITKIEEAAKPLGFD 371

Query: 363 VQKRNYKLKLQGENPGRKGQLAIATEVFEVTPSLYMVELRKSNGDTLEFHKFYHSISNGL 184
           +QK+NYK++++    GRKG L +ATEVF+V PSL++VEL+K+ GDTLEF KFY ++S  L
Sbjct: 372 IQKKNYKMRMENLKAGRKGNLNVATEVFQVAPSLHVVELKKAKGDTLEFQKFYRTLSTQL 431

Query: 183 KDVMWKPEGSI 151
           KDV+WK +G +
Sbjct: 432 KDVVWKCDGEV 442



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>CPK24_ARATH (Q9LDI3) CBL-interacting serine/threonine-protein kinase 24 (EC|
           2.7.11.1) (SNF1-related kinase 3.11) (SALT OVERLY
           SENSITIVE 2 protein)
          Length = 446

 Score =  139 bits (351), Expect = 4e-33
 Identities = 65/138 (47%), Positives = 98/138 (71%)
 Frame = -3

Query: 543 ERPAVMNAFELISTSQGLNLGTLFEKQTDSVKRETRFASRLPANEILSKIEAAAGPMGFN 364
           E P +MNAFE+I+ SQGLNL  LF+++ D VKR+TRF SR   +EI++ IEA A  MGF 
Sbjct: 305 EGPLMMNAFEMITLSQGLNLSALFDRRQDFVKRQTRFVSRREPSEIIANIEAVANSMGFK 364

Query: 363 VQKRNYKLKLQGENPGRKGQLAIATEVFEVTPSLYMVELRKSNGDTLEFHKFYHSISNGL 184
              RN+K +L+G +  + GQLA+  E++EV PSL+MV++RK+ G+TLE+HKFY  + + L
Sbjct: 365 SHTRNFKTRLEGLSSIKAGQLAVVIEIYEVAPSLFMVDVRKAAGETLEYHKFYKKLCSKL 424

Query: 183 KDVMWKPEGSIAEGDETR 130
           ++++W+    I + +  R
Sbjct: 425 ENIIWRATEGIPKSEILR 442



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>CIPK1_ARATH (Q8RWC9) CBL-interacting serine/threonine-protein kinase 1 (EC|
           2.7.11.1) (SOS2-like protein kinase PKS13) (SNF1-related
           kinase 3.16)
          Length = 444

 Score = 96.3 bits (238), Expect = 5e-20
 Identities = 55/125 (44%), Positives = 80/125 (64%), Gaps = 2/125 (1%)
 Frame = -3

Query: 543 ERPAVMNAFELISTSQGLNLGTLFEKQTDSVKRETRFASRLPANEILSKIEAAAGPMGFN 364
           + P ++NAF+LI  S  L+L   FE++  S +R  RF S   A ++L KIE A   MGF+
Sbjct: 313 DSPTIINAFQLIGMSSFLDLSGFFEQENVS-ERRIRFTSNSSAKDLLEKIETAVTEMGFS 371

Query: 363 VQKRNYKLKLQGENPGRKGQ--LAIATEVFEVTPSLYMVELRKSNGDTLEFHKFYHSISN 190
           VQK++ KL+++ E   +KGQ  L++  EVFE+ PSL +VELRKS GD+  + + Y  +  
Sbjct: 372 VQKKHAKLRVKQEERNQKGQVGLSVTAEVFEIKPSLNVVELRKSYGDSCLYRQLYERL-- 429

Query: 189 GLKDV 175
            LKDV
Sbjct: 430 -LKDV 433



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>CPK15_ARATH (P92937) CBL-interacting serine/threonine-protein kinase 15 (EC|
           2.7.11.1) (Serine/threonine-protein kinase ATPK10)
           (SOS2-like protein kinase PKS3) (SOS-interacting protein
           2) (SNF1-related kinase 3.1)
          Length = 421

 Score = 68.2 bits (165), Expect = 1e-11
 Identities = 41/108 (37%), Positives = 60/108 (55%), Gaps = 2/108 (1%)
 Frame = -3

Query: 528 MNAFELISTSQGLNLGTLFEKQTDSVKRETRFASRLPANEILSKIEAAAGPMGFNVQKRN 349
           +NAFE+IS S G +L  LFEK  +  K E RF S   A+EI  K+      +   V+K+ 
Sbjct: 304 LNAFEIISLSTGFDLSGLFEKGEE--KEEMRFTSNREASEITEKLVEIGKDLKMKVRKKE 361

Query: 348 Y--KLKLQGENPGRKGQLAIATEVFEVTPSLYMVELRKSNGDTLEFHK 211
           +  ++K+  E         +  EVFE+ PS +MV L+KS GDT E+ +
Sbjct: 362 HEWRVKMSAE------ATVVEAEVFEIAPSYHMVVLKKSGGDTAEYKR 403



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>CPK11_ARATH (O22932) CBL-interacting serine/threonine-protein kinase 11 (EC|
           2.7.11.1) (SOS2-like protein kinase PKS5)
           (SOS-interacting protein 4) (SNF1-related kinase 3.22)
          Length = 435

 Score = 45.4 bits (106), Expect = 1e-04
 Identities = 30/103 (29%), Positives = 50/103 (48%), Gaps = 3/103 (2%)
 Frame = -3

Query: 528 MNAFELISTSQGLNLGTLFEKQTDSVKRETRFASRLPANEILSKIEAAAGPMGFNVQKR- 352
           +NAF+LIS S GL+L  LF   ++S     RF S      +  ++E  A      ++K+ 
Sbjct: 307 LNAFDLISYSSGLDLSGLFAGCSNSSGESERFLSEKSPEMLAEEVEGFAREENLRMKKKK 366

Query: 351 --NYKLKLQGENPGRKGQLAIATEVFEVTPSLYMVELRKSNGD 229
              Y  +++G+N    G+  I   +  +   L +VE R+  GD
Sbjct: 367 EEEYGFEMEGQN----GKFGIGICISRLNDLLVVVEARRRGGD 405



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>CHK1_MOUSE (O35280) Serine/threonine-protein kinase Chk1 (EC 2.7.11.1)|
          Length = 476

 Score = 37.4 bits (85), Expect = 0.028
 Identities = 25/94 (26%), Positives = 43/94 (45%)
 Frame = -3

Query: 453 VKRETRFASRLPANEILSKIEAAAGPMGFNVQKRNYKLKLQGENPGRKGQLAIATEVFEV 274
           VKR TRF ++L A++    ++     +G+  +K             R  +L     + E+
Sbjct: 374 VKRMTRFFTKLDADKSYQCLKETFEKLGYQWKKSCMNQVTVSTTDRRNNKLIFKINLVEM 433

Query: 273 TPSLYMVELRKSNGDTLEFHKFYHSISNGLKDVM 172
              + +V+ R S GD LEF + +  I   L DV+
Sbjct: 434 DEKI-LVDFRLSKGDGLEFKRHFLKIKGKLSDVV 466



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>CHK1_HUMAN (O14757) Serine/threonine-protein kinase Chk1 (EC 2.7.11.1)|
          Length = 476

 Score = 37.0 bits (84), Expect = 0.036
 Identities = 24/94 (25%), Positives = 43/94 (45%)
 Frame = -3

Query: 453 VKRETRFASRLPANEILSKIEAAAGPMGFNVQKRNYKLKLQGENPGRKGQLAIATEVFEV 274
           VKR TRF ++L A++    ++     +G+  +K             R  +L     + E+
Sbjct: 374 VKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEM 433

Query: 273 TPSLYMVELRKSNGDTLEFHKFYHSISNGLKDVM 172
              + +V+ R S GD LEF + +  I   L D++
Sbjct: 434 DDKI-LVDFRLSKGDGLEFKRHFLKIKGKLIDIV 466



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>CHK1_RAT (Q91ZN7) Serine/threonine-protein kinase Chk1 (EC 2.7.11.1)|
          Length = 476

 Score = 36.2 bits (82), Expect = 0.062
 Identities = 24/94 (25%), Positives = 43/94 (45%)
 Frame = -3

Query: 453 VKRETRFASRLPANEILSKIEAAAGPMGFNVQKRNYKLKLQGENPGRKGQLAIATEVFEV 274
           VKR TRF ++L A++    ++     +G+  +K             R  +L     + E+
Sbjct: 374 VKRMTRFFTKLDADKSYQCLKETFEKLGYQWKKSCMNQVTVSTMDRRNNKLIFKINLVEM 433

Query: 273 TPSLYMVELRKSNGDTLEFHKFYHSISNGLKDVM 172
              + +V+ R S GD LEF + +  I   L D++
Sbjct: 434 DEKI-LVDFRLSKGDGLEFKRHFLKIKGKLSDIV 466



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>CHK1_CHICK (Q8AYC9) Serine/threonine-protein kinase Chk1 (EC 2.7.11.1)|
          Length = 476

 Score = 35.4 bits (80), Expect = 0.11
 Identities = 25/94 (26%), Positives = 41/94 (43%)
 Frame = -3

Query: 453 VKRETRFASRLPANEILSKIEAAAGPMGFNVQKRNYKLKLQGENPGRKGQLAIATEVFEV 274
           V+R TRF ++L A+     +      MG+  ++             R  +L     + E+
Sbjct: 374 VRRMTRFFTKLDADGSYRSLRDVCEKMGYGWKQSCTNQVTISTTDRRNNKLIFKVNLLEM 433

Query: 273 TPSLYMVELRKSNGDTLEFHKFYHSISNGLKDVM 172
             S  +V+ R S GD LEF + +  I   L DV+
Sbjct: 434 E-SRILVDFRLSKGDGLEFKRHFLKIKGKLSDVV 466



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>CHK1_XENLA (Q6DE87) Serine/threonine-protein kinase Chk1 (EC 2.7.11.1) (xChk1|
           protein)
          Length = 474

 Score = 32.3 bits (72), Expect = 0.89
 Identities = 23/94 (24%), Positives = 41/94 (43%)
 Frame = -3

Query: 453 VKRETRFASRLPANEILSKIEAAAGPMGFNVQKRNYKLKLQGENPGRKGQLAIATEVFEV 274
           VKR TRF +++ A    S +      MG+ ++K             R  +L     + E+
Sbjct: 373 VKRMTRFFTKVNAESSYSNLMDTCEKMGYVLKKSCANEVTLSTTDRRNNKLIFKVNLVEM 432

Query: 273 TPSLYMVELRKSNGDTLEFHKFYHSISNGLKDVM 172
              + +++ R S GD LEF + +  I   +  V+
Sbjct: 433 EDRI-LLDFRLSKGDGLEFKRHFLKIKKKMDAVV 465



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>SLH1_YEAST (P53327) Antiviral helicase SLH1 (EC 3.6.1.-) (SKI2-like helicase|
           1)
          Length = 1967

 Score = 32.0 bits (71), Expect = 1.2
 Identities = 23/63 (36%), Positives = 33/63 (52%)
 Frame = -3

Query: 417 ANEILSKIEAAAGPMGFNVQKRNYKLKLQGENPGRKGQLAIATEVFEVTPSLYMVELRKS 238
           A EI+ K      P  FN+Q R    +L G+    K ++ +AT+V   TP  + V  RK+
Sbjct: 359 AAEIVDKFSKKLAP--FNIQVR----ELTGDMQLTKAEI-LATQVIVTTPEKWDVVTRKA 411

Query: 237 NGD 229
           NGD
Sbjct: 412 NGD 414



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>CHK1_SCHPO (P34208) Serine/threonine-protein kinase chk1 (EC 2.7.11.1)|
           (Checkpoint kinase 1)
          Length = 496

 Score = 31.6 bits (70), Expect = 1.5
 Identities = 23/92 (25%), Positives = 42/92 (45%), Gaps = 3/92 (3%)
 Frame = -3

Query: 450 KRETRFASRLPANEILSKIEAAAGPMGFNVQK---RNYKLKLQGENPGRKGQLAIATEVF 280
           +R TRF SR     I+  +  +   +  +V     RN  +     +  RK  L    E+ 
Sbjct: 393 ERLTRFYSRASRETIIDHLYDSLRLLAISVTMKYVRNQTILYVNLHDKRKCLLQGVIELT 452

Query: 279 EVTPSLYMVELRKSNGDTLEFHKFYHSISNGL 184
            +  +L ++   K NGD LE+ KF+ ++ + +
Sbjct: 453 NLGHNLELINFIKRNGDPLEWRKFFKNVVSSI 484



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>TRM1_PYRAB (Q9V1P3) N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC|
           2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase)
           (tRNA 2,2-dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase)
          Length = 383

 Score = 28.9 bits (63), Expect = 9.9
 Identities = 24/85 (28%), Positives = 38/85 (44%), Gaps = 1/85 (1%)
 Frame = -3

Query: 447 RETRFASRLPANEI-LSKIEAAAGPMGFNVQKRNYKLKLQGENPGRKGQLAIATEVFEVT 271
           R  RFA   PA EI ++ I   A    +N+ K+N  L  +GE     G+  + +E     
Sbjct: 65  RGIRFALETPAEEIWMNDISEDA----YNLMKKNVLLNFKGELEESNGRAVLKSE----- 115

Query: 270 PSLYMVELRKSNGDTLEFHKFYHSI 196
               +V    +N    E H+++H I
Sbjct: 116 -KTLVVNHDDANRLMAEKHRYFHFI 139



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>PPCK_CHLCV (Q821M4) Phosphoenolpyruvate carboxykinase [GTP] (EC 4.1.1.32) (PEP|
           carboxykinase) (Phosphoenolpyruvate carboxylase) (PEPCK)
          Length = 600

 Score = 28.9 bits (63), Expect = 9.9
 Identities = 21/52 (40%), Positives = 25/52 (48%), Gaps = 15/52 (28%)
 Frame = -3

Query: 279 EVTPSLYMV-----------ELRKSNGDTLEFHKFYHSI----SNGLKDVMW 169
           E+T S Y+V           E+ KS G T  FHK  HS+    S G KDV W
Sbjct: 140 ELTDSPYVVCSMKIMTRMGAEVLKSLGTTGSFHKCLHSVGAPLSPGEKDVAW 191



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>Y861_METJA (Q58271) Hypothetical UPF0036 protein MJ0861|
          Length = 448

 Score = 28.9 bits (63), Expect = 9.9
 Identities = 18/60 (30%), Positives = 29/60 (48%)
 Frame = -3

Query: 294 ATEVFEVTPSLYMVELRKSNGDTLEFHKFYHSISNGLKDVMWKPEGSIAEGDETRHRRSP 115
           A E  ++TP++           TLEF +  HSI + +  V+  P GSI  G++ +    P
Sbjct: 132 AGESIDLTPNI-----------TLEFIRITHSIPDSVLPVLHTPYGSIVYGNDFKFDNFP 180



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>ILVH_ECOLI (P00894) Acetolactate synthase isozyme III small subunit (EC|
           2.2.1.6) (AHAS-III) (Acetohydroxy-acid synthase III
           small subunit) (ALS-III)
          Length = 163

 Score = 28.9 bits (63), Expect = 9.9
 Identities = 26/107 (24%), Positives = 47/107 (43%), Gaps = 16/107 (14%)
 Frame = -3

Query: 504 TSQGLNLGTLFEKQTDSVKRETRFASRLPANEILSKIEAAAGPM-----------GFNVQ 358
           + +G N+ +L    TD           +   ++L +IE     +           G +V+
Sbjct: 24  SQRGYNIESLTVAPTDDPTLSRMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVE 83

Query: 357 KRNYKLKLQGENPGRKGQLAIATEVF-----EVTPSLYMVELRKSNG 232
           +    +K+Q    GR  ++   TE+F     +VTPSLY V+L  ++G
Sbjct: 84  REIMLVKIQASGYGRD-EVKRNTEIFRGQIIDVTPSLYTVQLAGTSG 129


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 80,397,403
Number of Sequences: 219361
Number of extensions: 1662508
Number of successful extensions: 4165
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 4031
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4157
length of database: 80,573,946
effective HSP length: 105
effective length of database: 57,541,041
effective search space used: 4373119116
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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