ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd17g04
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1GSTF1_MAIZE (P12653) Glutathione S-transferase I (EC 2.5.1.18) (... 61 7e-10
2GSTH2_ORYSA (O82451) Probable glutathione S-transferase GSTF2 (E... 60 1e-09
3GSTH1_ORYSA (O65857) Probable glutathione S-transferase GSTF1 (E... 58 6e-09
4GSTF2_WHEAT (P30111) Glutathione S-transferase 2 (EC 2.5.1.18) (... 44 1e-04
5GSTF1_WHEAT (P30110) Glutathione S-transferase 1 (EC 2.5.1.18) (... 44 2e-04
6GSTF4_MAIZE (P46420) Glutathione S-transferase IV (EC 2.5.1.18) ... 40 0.001
7GSTF1_ARATH (P42760) Glutathione S-transferase 1 (EC 2.5.1.18) (... 36 0.025
8GSTF_HYOMU (P46423) Glutathione S-transferase (EC 2.5.1.18) (GST... 35 0.056
9GSTF3_MAIZE (P04907) Glutathione S-transferase III (EC 2.5.1.18)... 34 0.095
10GST11_ARATH (Q9SRY5) Glutathione S-transferase 11 (EC 2.5.1.18) ... 32 0.62
11MARK1_RAT (O08678) Serine/threonine-protein kinase MARK1 (EC 2.7... 30 1.4
12MARK1_MOUSE (Q8VHJ5) Serine/threonine-protein kinase MARK1 (EC 2... 30 1.4
13GSTF_SILCU (Q04522) Glutathione S-transferase (EC 2.5.1.18) (GST... 30 2.3
14GSTF2_TOBAC (P46440) Glutathione S-transferase APIC (EC 2.5.1.18... 28 6.8
15GSTF1_TOBAC (P30109) Glutathione S-transferase PARB (EC 2.5.1.18... 28 6.8
16DCMA_METS1 (P43387) Dichloromethane dehalogenase (EC 4.5.1.3) (D... 28 8.9
17HIW_DROME (Q9NB71) Ubiquitin ligase protein highwire (EC 6.3.2.-... 28 8.9

>GSTF1_MAIZE (P12653) Glutathione S-transferase I (EC 2.5.1.18) (GST-I) (GST-29)|
           (GST class-phi)
          Length = 213

 Score = 61.2 bits (147), Expect = 7e-10
 Identities = 26/33 (78%), Positives = 30/33 (90%)
 Frame = -1

Query: 166 YASLFDAYPHVKAWWTDLLTRPSVQKVAALMQP 68
           YAS+ DAYPHVKAWW+ L+ RPSVQKVAALM+P
Sbjct: 179 YASVLDAYPHVKAWWSGLMERPSVQKVAALMKP 211



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>GSTH2_ORYSA (O82451) Probable glutathione S-transferase GSTF2 (EC 2.5.1.18)|
           (GST-II)
          Length = 214

 Score = 60.5 bits (145), Expect = 1e-09
 Identities = 25/33 (75%), Positives = 30/33 (90%)
 Frame = -1

Query: 166 YASLFDAYPHVKAWWTDLLTRPSVQKVAALMQP 68
           +AS+ DAYPHVKAWWTDL+ RPS QKVA+LM+P
Sbjct: 180 HASVLDAYPHVKAWWTDLMARPSSQKVASLMKP 212



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>GSTH1_ORYSA (O65857) Probable glutathione S-transferase GSTF1 (EC 2.5.1.18)|
           (GST-I)
          Length = 219

 Score = 58.2 bits (139), Expect = 6e-09
 Identities = 24/33 (72%), Positives = 29/33 (87%)
 Frame = -1

Query: 166 YASLFDAYPHVKAWWTDLLTRPSVQKVAALMQP 68
           YASLFD+YPHVKAWW  L+ RPSV+K+AA+M P
Sbjct: 184 YASLFDSYPHVKAWWERLMARPSVKKLAAVMAP 216



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>GSTF2_WHEAT (P30111) Glutathione S-transferase 2 (EC 2.5.1.18) (GST class-phi)|
          Length = 291

 Score = 43.9 bits (102), Expect = 1e-04
 Identities = 18/31 (58%), Positives = 22/31 (70%)
 Frame = -1

Query: 166 YASLFDAYPHVKAWWTDLLTRPSVQKVAALM 74
           YA +FD YP VKAWW  L+ RP+VQ+V   M
Sbjct: 189 YAKVFDEYPKVKAWWEMLMARPAVQRVCKHM 219



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>GSTF1_WHEAT (P30110) Glutathione S-transferase 1 (EC 2.5.1.18) (GST class-phi)|
          Length = 229

 Score = 43.5 bits (101), Expect = 2e-04
 Identities = 18/31 (58%), Positives = 22/31 (70%)
 Frame = -1

Query: 166 YASLFDAYPHVKAWWTDLLTRPSVQKVAALM 74
           YA +FD YP VKAWW  L+ RP+VQ+V   M
Sbjct: 189 YAKVFDDYPKVKAWWEMLMARPAVQRVCKHM 219



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>GSTF4_MAIZE (P46420) Glutathione S-transferase IV (EC 2.5.1.18) (GST-IV)|
           (GST-27) (GST class-phi)
          Length = 222

 Score = 40.4 bits (93), Expect = 0.001
 Identities = 17/31 (54%), Positives = 19/31 (61%)
 Frame = -1

Query: 166 YASLFDAYPHVKAWWTDLLTRPSVQKVAALM 74
           YA+L  A PHV AWW  L  RP+  KVA  M
Sbjct: 181 YAALVHALPHVSAWWQGLAARPAANKVAQFM 211



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>GSTF1_ARATH (P42760) Glutathione S-transferase 1 (EC 2.5.1.18) (GST class-phi)|
          Length = 208

 Score = 36.2 bits (82), Expect = 0.025
 Identities = 15/24 (62%), Positives = 17/24 (70%)
 Frame = -1

Query: 157 LFDAYPHVKAWWTDLLTRPSVQKV 86
           LFD  PHV AW  D+ +RPS QKV
Sbjct: 184 LFDERPHVSAWVADITSRPSAQKV 207



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>GSTF_HYOMU (P46423) Glutathione S-transferase (EC 2.5.1.18) (GST class-phi)|
           (25 kDa auxin-binding protein)
          Length = 212

 Score = 35.0 bits (79), Expect = 0.056
 Identities = 15/30 (50%), Positives = 18/30 (60%)
 Frame = -1

Query: 160 SLFDAYPHVKAWWTDLLTRPSVQKVAALMQ 71
           SLFD+ PHV AW  D+L RP+  K     Q
Sbjct: 183 SLFDSRPHVSAWCADILARPAWSKAIEYKQ 212



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>GSTF3_MAIZE (P04907) Glutathione S-transferase III (EC 2.5.1.18) (GST-III) (GST|
           class-phi)
          Length = 221

 Score = 34.3 bits (77), Expect = 0.095
 Identities = 16/28 (57%), Positives = 19/28 (67%), Gaps = 1/28 (3%)
 Frame = -1

Query: 148 AYPHVKAWWTDLLTRPSVQK-VAALMQP 68
           A PHVKAWW  +  RP+ QK VAA+  P
Sbjct: 187 ARPHVKAWWEAIAARPAFQKTVAAIPLP 214



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>GST11_ARATH (Q9SRY5) Glutathione S-transferase 11 (EC 2.5.1.18) (GST class-phi)|
          Length = 209

 Score = 31.6 bits (70), Expect = 0.62
 Identities = 13/24 (54%), Positives = 16/24 (66%)
 Frame = -1

Query: 157 LFDAYPHVKAWWTDLLTRPSVQKV 86
           LF   PHV AW  D+ +RPS +KV
Sbjct: 185 LFAERPHVSAWVADITSRPSAKKV 208



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>MARK1_RAT (O08678) Serine/threonine-protein kinase MARK1 (EC 2.7.11.1)|
           (MAP/microtubule affinity-regulating kinase 1)
          Length = 793

 Score = 30.4 bits (67), Expect = 1.4
 Identities = 14/33 (42%), Positives = 18/33 (54%)
 Frame = +1

Query: 67  MAASAQRPSGRTASSAGQSTRPSHAGKRQTEMH 165
           M+AS+   +G T +SAG S RP H     T  H
Sbjct: 528 MSASSMSSTGSTVASAGPSARPRHQKSMSTSGH 560



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>MARK1_MOUSE (Q8VHJ5) Serine/threonine-protein kinase MARK1 (EC 2.7.11.1)|
           (MAP/microtubule affinity-regulating kinase 1) (ELKL
           motif serine/threonine-protein kinase 3)
          Length = 795

 Score = 30.4 bits (67), Expect = 1.4
 Identities = 14/33 (42%), Positives = 18/33 (54%)
 Frame = +1

Query: 67  MAASAQRPSGRTASSAGQSTRPSHAGKRQTEMH 165
           M+AS+   +G T +SAG S RP H     T  H
Sbjct: 528 MSASSMSSAGSTVASAGPSARPRHQKSMSTSGH 560



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>GSTF_SILCU (Q04522) Glutathione S-transferase (EC 2.5.1.18) (GST class-phi)|
          Length = 216

 Score = 29.6 bits (65), Expect = 2.3
 Identities = 13/29 (44%), Positives = 17/29 (58%)
 Frame = -1

Query: 157 LFDAYPHVKAWWTDLLTRPSVQKVAALMQ 71
           LF+   HV AW   +L RPS +K  AL +
Sbjct: 186 LFEERAHVSAWCKKILARPSWEKTLALQK 214



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>GSTF2_TOBAC (P46440) Glutathione S-transferase APIC (EC 2.5.1.18) (GST|
           class-phi)
          Length = 213

 Score = 28.1 bits (61), Expect = 6.8
 Identities = 10/20 (50%), Positives = 14/20 (70%)
 Frame = -1

Query: 157 LFDAYPHVKAWWTDLLTRPS 98
           +FD+ P V AW  D+L RP+
Sbjct: 184 VFDSRPRVSAWCADILARPA 203



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>GSTF1_TOBAC (P30109) Glutathione S-transferase PARB (EC 2.5.1.18) (GST|
           class-phi)
          Length = 213

 Score = 28.1 bits (61), Expect = 6.8
 Identities = 10/20 (50%), Positives = 14/20 (70%)
 Frame = -1

Query: 157 LFDAYPHVKAWWTDLLTRPS 98
           +FD+ P V AW  D+L RP+
Sbjct: 184 VFDSRPRVSAWCADILARPA 203



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>DCMA_METS1 (P43387) Dichloromethane dehalogenase (EC 4.5.1.3) (DCM|
           dehalogenase)
          Length = 266

 Score = 27.7 bits (60), Expect = 8.9
 Identities = 10/27 (37%), Positives = 19/27 (70%)
 Frame = -1

Query: 160 SLFDAYPHVKAWWTDLLTRPSVQKVAA 80
           S++D++P VKAW+  ++ R   + V+A
Sbjct: 188 SMWDSHPKVKAWFARMMDREHAKTVSA 214



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>HIW_DROME (Q9NB71) Ubiquitin ligase protein highwire (EC 6.3.2.-) (Protein|
            pam/highwire/rpm-1)
          Length = 5233

 Score = 27.7 bits (60), Expect = 8.9
 Identities = 16/40 (40%), Positives = 20/40 (50%)
 Frame = +1

Query: 37   FDEQAPETKIMAASAQRPSGRTASSAGQSTRPSHAGKRQT 156
            F+EQ PE    AA+A   +  T  SAG   R    GK+ T
Sbjct: 1276 FEEQLPEVGSSAAAAAVAAPGTPVSAGSVPRSRRGGKQGT 1315


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.307    0.118    0.331 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,000,102
Number of Sequences: 219361
Number of extensions: 339850
Number of successful extensions: 842
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 835
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 842
length of database: 80,573,946
effective HSP length: 30
effective length of database: 73,993,116
effective search space used: 1775834784
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
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