ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd17g03
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1GSTF1_MAIZE (P12653) Glutathione S-transferase I (EC 2.5.1.18) (... 69 3e-12
2GSTH2_ORYSA (O82451) Probable glutathione S-transferase GSTF2 (E... 68 5e-12
3GSTH1_ORYSA (O65857) Probable glutathione S-transferase GSTF1 (E... 63 2e-10
4GSTF2_WHEAT (P30111) Glutathione S-transferase 2 (EC 2.5.1.18) (... 49 4e-06
5GSTF1_WHEAT (P30110) Glutathione S-transferase 1 (EC 2.5.1.18) (... 48 6e-06
6GSTF4_MAIZE (P46420) Glutathione S-transferase IV (EC 2.5.1.18) ... 44 1e-04
7GSTF1_ARATH (P42760) Glutathione S-transferase 1 (EC 2.5.1.18) (... 40 0.002
8GSTF_HYOMU (P46423) Glutathione S-transferase (EC 2.5.1.18) (GST... 35 0.038
9GST11_ARATH (Q9SRY5) Glutathione S-transferase 11 (EC 2.5.1.18) ... 35 0.050
10GSTF3_MAIZE (P04907) Glutathione S-transferase III (EC 2.5.1.18)... 34 0.085
11GSTF_SILCU (Q04522) Glutathione S-transferase (EC 2.5.1.18) (GST... 32 0.42
12HEMN_PSEAE (P77915) Oxygen-independent coproporphyrinogen III ox... 30 1.2
13MARK1_RAT (O08678) Serine/threonine-protein kinase MARK1 (EC 2.7... 30 2.1
14MARK1_MOUSE (Q8VHJ5) Serine/threonine-protein kinase MARK1 (EC 2... 30 2.1
15GSTF2_TOBAC (P46440) Glutathione S-transferase APIC (EC 2.5.1.18... 29 2.7
16GSTF1_TOBAC (P30109) Glutathione S-transferase PARB (EC 2.5.1.18... 28 6.1
17FRDB_HELPY (O06914) Fumarate reductase iron-sulfur protein (EC 1... 28 8.0
18DCMA_METS1 (P43387) Dichloromethane dehalogenase (EC 4.5.1.3) (D... 28 8.0

>GSTF1_MAIZE (P12653) Glutathione S-transferase I (EC 2.5.1.18) (GST-I) (GST-29)|
           (GST class-phi)
          Length = 213

 Score = 68.9 bits (167), Expect = 3e-12
 Identities = 30/36 (83%), Positives = 33/36 (91%)
 Frame = -1

Query: 270 ATPYASLFDAYPHVKAWWTDLLTRPSVQKVAALMKP 163
           ATPYAS+ DAYPHVKAWW+ L+ RPSVQKVAALMKP
Sbjct: 176 ATPYASVLDAYPHVKAWWSGLMERPSVQKVAALMKP 211



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>GSTH2_ORYSA (O82451) Probable glutathione S-transferase GSTF2 (EC 2.5.1.18)|
           (GST-II)
          Length = 214

 Score = 68.2 bits (165), Expect = 5e-12
 Identities = 29/36 (80%), Positives = 33/36 (91%)
 Frame = -1

Query: 270 ATPYASLFDAYPHVKAWWTDLLTRPSVQKVAALMKP 163
           ATP+AS+ DAYPHVKAWWTDL+ RPS QKVA+LMKP
Sbjct: 177 ATPHASVLDAYPHVKAWWTDLMARPSSQKVASLMKP 212



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>GSTH1_ORYSA (O65857) Probable glutathione S-transferase GSTF1 (EC 2.5.1.18)|
           (GST-I)
          Length = 219

 Score = 62.8 bits (151), Expect = 2e-10
 Identities = 26/35 (74%), Positives = 31/35 (88%)
 Frame = -1

Query: 267 TPYASLFDAYPHVKAWWTDLLTRPSVQKVAALMKP 163
           TPYASLFD+YPHVKAWW  L+ RPSV+K+AA+M P
Sbjct: 182 TPYASLFDSYPHVKAWWERLMARPSVKKLAAVMAP 216



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>GSTF2_WHEAT (P30111) Glutathione S-transferase 2 (EC 2.5.1.18) (GST class-phi)|
          Length = 291

 Score = 48.5 bits (114), Expect = 4e-06
 Identities = 20/33 (60%), Positives = 24/33 (72%)
 Frame = -1

Query: 267 TPYASLFDAYPHVKAWWTDLLTRPSVQKVAALM 169
           TPYA +FD YP VKAWW  L+ RP+VQ+V   M
Sbjct: 187 TPYAKVFDEYPKVKAWWEMLMARPAVQRVCKHM 219



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>GSTF1_WHEAT (P30110) Glutathione S-transferase 1 (EC 2.5.1.18) (GST class-phi)|
          Length = 229

 Score = 48.1 bits (113), Expect = 6e-06
 Identities = 20/33 (60%), Positives = 24/33 (72%)
 Frame = -1

Query: 267 TPYASLFDAYPHVKAWWTDLLTRPSVQKVAALM 169
           TPYA +FD YP VKAWW  L+ RP+VQ+V   M
Sbjct: 187 TPYAKVFDDYPKVKAWWEMLMARPAVQRVCKHM 219



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>GSTF4_MAIZE (P46420) Glutathione S-transferase IV (EC 2.5.1.18) (GST-IV)|
           (GST-27) (GST class-phi)
          Length = 222

 Score = 43.5 bits (101), Expect = 1e-04
 Identities = 19/34 (55%), Positives = 21/34 (61%)
 Frame = -1

Query: 270 ATPYASLFDAYPHVKAWWTDLLTRPSVQKVAALM 169
           AT YA+L  A PHV AWW  L  RP+  KVA  M
Sbjct: 178 ATEYAALVHALPHVSAWWQGLAARPAANKVAQFM 211



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>GSTF1_ARATH (P42760) Glutathione S-transferase 1 (EC 2.5.1.18) (GST class-phi)|
          Length = 208

 Score = 39.7 bits (91), Expect = 0.002
 Identities = 17/29 (58%), Positives = 19/29 (65%)
 Frame = -1

Query: 267 TPYASLFDAYPHVKAWWTDLLTRPSVQKV 181
           TP   LFD  PHV AW  D+ +RPS QKV
Sbjct: 179 TPTKKLFDERPHVSAWVADITSRPSAQKV 207



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>GSTF_HYOMU (P46423) Glutathione S-transferase (EC 2.5.1.18) (GST class-phi)|
           (25 kDa auxin-binding protein)
          Length = 212

 Score = 35.4 bits (80), Expect = 0.038
 Identities = 15/28 (53%), Positives = 18/28 (64%)
 Frame = -1

Query: 267 TPYASLFDAYPHVKAWWTDLLTRPSVQK 184
           T   SLFD+ PHV AW  D+L RP+  K
Sbjct: 179 TKVKSLFDSRPHVSAWCADILARPAWSK 206



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>GST11_ARATH (Q9SRY5) Glutathione S-transferase 11 (EC 2.5.1.18) (GST class-phi)|
          Length = 209

 Score = 35.0 bits (79), Expect = 0.050
 Identities = 15/29 (51%), Positives = 18/29 (62%)
 Frame = -1

Query: 267 TPYASLFDAYPHVKAWWTDLLTRPSVQKV 181
           TP   LF   PHV AW  D+ +RPS +KV
Sbjct: 180 TPTKKLFAERPHVSAWVADITSRPSAKKV 208



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>GSTF3_MAIZE (P04907) Glutathione S-transferase III (EC 2.5.1.18) (GST-III) (GST|
           class-phi)
          Length = 221

 Score = 34.3 bits (77), Expect = 0.085
 Identities = 16/28 (57%), Positives = 19/28 (67%), Gaps = 1/28 (3%)
 Frame = -1

Query: 243 AYPHVKAWWTDLLTRPSVQK-VAALMKP 163
           A PHVKAWW  +  RP+ QK VAA+  P
Sbjct: 187 ARPHVKAWWEAIAARPAFQKTVAAIPLP 214



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>GSTF_SILCU (Q04522) Glutathione S-transferase (EC 2.5.1.18) (GST class-phi)|
          Length = 216

 Score = 32.0 bits (71), Expect = 0.42
 Identities = 15/34 (44%), Positives = 18/34 (52%)
 Frame = -1

Query: 267 TPYASLFDAYPHVKAWWTDLLTRPSVQKVAALMK 166
           T    LF+   HV AW   +L RPS +K  AL K
Sbjct: 181 TQVKKLFEERAHVSAWCKKILARPSWEKTLALQK 214



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>HEMN_PSEAE (P77915) Oxygen-independent coproporphyrinogen III oxidase (EC|
           1.3.99.22) (Coproporphyrinogenase) (Coprogen oxidase)
          Length = 460

 Score = 30.4 bits (67), Expect = 1.2
 Identities = 15/47 (31%), Positives = 25/47 (53%)
 Frame = +1

Query: 52  LNIQHQDTSQEHTLGKAHIGGDTPRLISTNKPQKLRSWLHQRSDLLD 192
           + I  +  S+E  + + H GG TP  +S  + ++L S L    +LLD
Sbjct: 94  IEIVSRHLSREQVVEQLHFGGGTPTFLSPGQLRELMSQLRTHLNLLD 140



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>MARK1_RAT (O08678) Serine/threonine-protein kinase MARK1 (EC 2.7.11.1)|
           (MAP/microtubule affinity-regulating kinase 1)
          Length = 793

 Score = 29.6 bits (65), Expect = 2.1
 Identities = 13/35 (37%), Positives = 19/35 (54%)
 Frame = +3

Query: 162 MASSAQRPSGRTASSAGQSTRPSHAGKRQTEMHRV 266
           M++S+   +G T +SAG S RP H     T  H +
Sbjct: 528 MSASSMSSTGSTVASAGPSARPRHQKSMSTSGHPI 562



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>MARK1_MOUSE (Q8VHJ5) Serine/threonine-protein kinase MARK1 (EC 2.7.11.1)|
           (MAP/microtubule affinity-regulating kinase 1) (ELKL
           motif serine/threonine-protein kinase 3)
          Length = 795

 Score = 29.6 bits (65), Expect = 2.1
 Identities = 13/35 (37%), Positives = 19/35 (54%)
 Frame = +3

Query: 162 MASSAQRPSGRTASSAGQSTRPSHAGKRQTEMHRV 266
           M++S+   +G T +SAG S RP H     T  H +
Sbjct: 528 MSASSMSSAGSTVASAGPSARPRHQKSMSTSGHPI 562



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>GSTF2_TOBAC (P46440) Glutathione S-transferase APIC (EC 2.5.1.18) (GST|
           class-phi)
          Length = 213

 Score = 29.3 bits (64), Expect = 2.7
 Identities = 11/26 (42%), Positives = 16/26 (61%)
 Frame = -1

Query: 270 ATPYASLFDAYPHVKAWWTDLLTRPS 193
           +T    +FD+ P V AW  D+L RP+
Sbjct: 178 STKVKEVFDSRPRVSAWCADILARPA 203



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>GSTF1_TOBAC (P30109) Glutathione S-transferase PARB (EC 2.5.1.18) (GST|
           class-phi)
          Length = 213

 Score = 28.1 bits (61), Expect = 6.1
 Identities = 10/20 (50%), Positives = 14/20 (70%)
 Frame = -1

Query: 252 LFDAYPHVKAWWTDLLTRPS 193
           +FD+ P V AW  D+L RP+
Sbjct: 184 VFDSRPRVSAWCADILARPA 203



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>FRDB_HELPY (O06914) Fumarate reductase iron-sulfur protein (EC 1.3.99.1)|
          Length = 245

 Score = 27.7 bits (60), Expect = 8.0
 Identities = 13/41 (31%), Positives = 21/41 (51%)
 Frame = +1

Query: 148 QKLRSWLHQRSDLLDGRPRQQVSPPGLHMRVSVKQRCIGCG 270
           +++ SW H + ++   RP ++V P      V    RCI CG
Sbjct: 119 KRVESWAHSKEEVDITRPEKRVEPDEA-QEVFELDRCIECG 158



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>DCMA_METS1 (P43387) Dichloromethane dehalogenase (EC 4.5.1.3) (DCM|
           dehalogenase)
          Length = 266

 Score = 27.7 bits (60), Expect = 8.0
 Identities = 10/27 (37%), Positives = 19/27 (70%)
 Frame = -1

Query: 255 SLFDAYPHVKAWWTDLLTRPSVQKVAA 175
           S++D++P VKAW+  ++ R   + V+A
Sbjct: 188 SMWDSHPKVKAWFARMMDREHAKTVSA 214


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 40,049,647
Number of Sequences: 219361
Number of extensions: 732580
Number of successful extensions: 2120
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 2035
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2120
length of database: 80,573,946
effective HSP length: 65
effective length of database: 66,315,481
effective search space used: 1591571544
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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