ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd16k10
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1AAT_PYRHO (O58489) Aspartate aminotransferase (EC 2.6.1.1) (Tran... 33 0.19
2AAT_SYNY3 (Q55128) Aspartate aminotransferase (EC 2.6.1.1) (Tran... 32 0.56
3AAT_PYRAB (Q9V0L2) Aspartate aminotransferase (EC 2.6.1.1) (Tran... 31 0.73
4NEC2_RAT (P28841) Neuroendocrine convertase 2 precursor (EC 3.4.... 30 2.1
5NEC2_PIG (Q03333) Neuroendocrine convertase 2 precursor (EC 3.4.... 30 2.1
6NEC2_MOUSE (P21661) Neuroendocrine convertase 2 precursor (EC 3.... 30 2.1
7NEC2_HUMAN (P16519) Neuroendocrine convertase 2 precursor (EC 3.... 30 2.1
8YE5A_SCHPO (O14174) Hypothetical protein C4D7.10c in chromosome I 29 2.8
9YBDL_ECOLI (P77806) Aminotransferase ybdL (EC 2.6.1.-) 29 3.6
10V70K_TYMVA (P20131) 69 kDa protein 28 4.7
11DSBE_CHRVI (Q46476) Thiol:disulfide interchange protein dsbE (Cy... 28 6.1
12SYW_CLOAB (Q97LD6) Tryptophanyl-tRNA synthetase (EC 6.1.1.2) (Tr... 28 6.1
13WNK4_MOUSE (Q80UE6) Serine/threonine-protein kinase WNK4 (EC 2.7... 28 8.0
14TENS1_HUMAN (Q9HBL0) Tensin-1 28 8.0
15AGI_HORVU (P15312) Root-specific lectin precursor 28 8.0
16NAS35_CAEEL (P98060) Zinc metalloproteinase nas-35 precursor (EC... 28 8.0
17HEP_DROME (Q23977) Dual specificity mitogen-activated protein ki... 28 8.0
18YFDZ_ECOLI (P77434) Hypothetical aminotransferase yfdZ (EC 2.6.1.-) 28 8.0
19PALY_MALDO (P35512) Phenylalanine ammonia-lyase (EC 4.3.1.5) (Fr... 28 8.0
20AAT_AQUAE (O67781) Aspartate aminotransferase (EC 2.6.1.1) (Tran... 28 8.0

>AAT_PYRHO (O58489) Aspartate aminotransferase (EC 2.6.1.1) (Transaminase A)|
           (AspAT)
          Length = 391

 Score = 33.1 bits (74), Expect = 0.19
 Identities = 19/71 (26%), Positives = 33/71 (46%)
 Frame = -2

Query: 217 EXIRERVQDLVINRKLLVEAMSPLGEDAVKGGXGAIYLWAKLPDNCKDDFEVVRWLATKH 38
           + + E  ++    RK++V+ +  +    VK   GA Y++  +        +   WL  K 
Sbjct: 288 KAVEEMKKEYNERRKIVVKRLKNMPGIKVKEPKGAFYVFPNISGTGMSSEKFSEWLLEKA 347

Query: 37  GVAVIPGSASG 5
            V VIPG+A G
Sbjct: 348 RVVVIPGTAFG 358



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>AAT_SYNY3 (Q55128) Aspartate aminotransferase (EC 2.6.1.1) (Transaminase A)|
           (ASPAT)
          Length = 389

 Score = 31.6 bits (70), Expect = 0.56
 Identities = 18/69 (26%), Positives = 32/69 (46%)
 Frame = -2

Query: 211 IRERVQDLVINRKLLVEAMSPLGEDAVKGGXGAIYLWAKLPDNCKDDFEVVRWLATKHGV 32
           +   V+     R+++VE ++ +   +     GA Y++  +     +  E    L   H V
Sbjct: 290 VETMVKAFTERRQVIVEGINQIAGLSCPNPKGAFYVFVDIAKTGLNSLEFSARLLESHQV 349

Query: 31  AVIPGSASG 5
           AVIPG+A G
Sbjct: 350 AVIPGAAFG 358



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>AAT_PYRAB (Q9V0L2) Aspartate aminotransferase (EC 2.6.1.1) (Transaminase A)|
           (AspAT)
          Length = 389

 Score = 31.2 bits (69), Expect = 0.73
 Identities = 20/71 (28%), Positives = 36/71 (50%)
 Frame = -2

Query: 217 EXIRERVQDLVINRKLLVEAMSPLGEDAVKGGXGAIYLWAKLPDNCKDDFEVVRWLATKH 38
           + + E  ++    RKL+ + ++ +G   VK   GA Y++ ++ D      E    +  + 
Sbjct: 285 KAVEEMRKEYDRRRKLVWKRLNEMGLPTVKP-KGAFYIFPRIKDTGLTSKEFSELMLMEA 343

Query: 37  GVAVIPGSASG 5
            VAV+PGSA G
Sbjct: 344 KVAVVPGSAFG 354



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>NEC2_RAT (P28841) Neuroendocrine convertase 2 precursor (EC 3.4.21.94) (NEC|
           2) (PC2) (Prohormone convertase 2) (Proprotein
           convertase 2) (KEX2-like endoprotease 2)
          Length = 637

 Score = 29.6 bits (65), Expect = 2.1
 Identities = 20/61 (32%), Positives = 31/61 (50%)
 Frame = -2

Query: 253 QRLALYSLXAGPEXIRERVQDLVINRKLLVEAMSPLGEDAVKGGXGAIYLWAKLPDNCKD 74
           Q + +YS   GP    + V      R+L ++AM+  G +  +GG G+IY+WA       D
Sbjct: 259 QLIDIYSASWGPTDNGKTVDG---PRELTLQAMAD-GVNKGRGGKGSIYVWASGDGGSYD 314

Query: 73  D 71
           D
Sbjct: 315 D 315



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>NEC2_PIG (Q03333) Neuroendocrine convertase 2 precursor (EC 3.4.21.94) (NEC|
           2) (PC2) (Prohormone convertase 2) (Proprotein
           convertase 2) (KEX2-like endoprotease 2)
          Length = 638

 Score = 29.6 bits (65), Expect = 2.1
 Identities = 20/61 (32%), Positives = 31/61 (50%)
 Frame = -2

Query: 253 QRLALYSLXAGPEXIRERVQDLVINRKLLVEAMSPLGEDAVKGGXGAIYLWAKLPDNCKD 74
           Q + +YS   GP    + V      R+L ++AM+  G +  +GG G+IY+WA       D
Sbjct: 260 QLIDIYSASWGPTDNGKTVDG---PRELTLQAMAD-GVNKGRGGKGSIYVWASGDGGSYD 315

Query: 73  D 71
           D
Sbjct: 316 D 316



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>NEC2_MOUSE (P21661) Neuroendocrine convertase 2 precursor (EC 3.4.21.94) (NEC|
           2) (PC2) (Prohormone convertase 2) (Proprotein
           convertase 2) (KEX2-like endoprotease 2)
          Length = 637

 Score = 29.6 bits (65), Expect = 2.1
 Identities = 20/61 (32%), Positives = 31/61 (50%)
 Frame = -2

Query: 253 QRLALYSLXAGPEXIRERVQDLVINRKLLVEAMSPLGEDAVKGGXGAIYLWAKLPDNCKD 74
           Q + +YS   GP    + V      R+L ++AM+  G +  +GG G+IY+WA       D
Sbjct: 259 QLIDIYSASWGPTDNGKTVDG---PRELTLQAMAD-GVNKGRGGKGSIYVWASGDGGSYD 314

Query: 73  D 71
           D
Sbjct: 315 D 315



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>NEC2_HUMAN (P16519) Neuroendocrine convertase 2 precursor (EC 3.4.21.94) (NEC|
           2) (PC2) (Prohormone convertase 2) (Proprotein
           convertase 2) (KEX2-like endoprotease 2)
          Length = 638

 Score = 29.6 bits (65), Expect = 2.1
 Identities = 20/61 (32%), Positives = 31/61 (50%)
 Frame = -2

Query: 253 QRLALYSLXAGPEXIRERVQDLVINRKLLVEAMSPLGEDAVKGGXGAIYLWAKLPDNCKD 74
           Q + +YS   GP    + V      R+L ++AM+  G +  +GG G+IY+WA       D
Sbjct: 260 QLIDIYSASWGPTDNGKTVDG---PRELTLQAMAD-GVNKGRGGKGSIYVWASGDGGSYD 315

Query: 73  D 71
           D
Sbjct: 316 D 316



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>YE5A_SCHPO (O14174) Hypothetical protein C4D7.10c in chromosome I|
          Length = 473

 Score = 29.3 bits (64), Expect = 2.8
 Identities = 10/18 (55%), Positives = 12/18 (66%)
 Frame = +2

Query: 176 PVYHEILHPFPDXLWASL 229
           PVYH +L P P+ LW  L
Sbjct: 174 PVYHTVLRPTPETLWQDL 191



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>YBDL_ECOLI (P77806) Aminotransferase ybdL (EC 2.6.1.-)|
          Length = 386

 Score = 28.9 bits (63), Expect = 3.6
 Identities = 11/19 (57%), Positives = 13/19 (68%)
 Frame = -2

Query: 76  DDFEVVRWLATKHGVAVIP 20
           DD E  +WL  +HGVA IP
Sbjct: 333 DDVEFCQWLTQEHGVAAIP 351



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>V70K_TYMVA (P20131) 69 kDa protein|
          Length = 628

 Score = 28.5 bits (62), Expect = 4.7
 Identities = 19/58 (32%), Positives = 22/58 (37%)
 Frame = +3

Query: 3   PPLALPGITATPCLVASHLTTSKSSLQLSGSLAQR*MAPXPPLTASSPRGDIASTSSF 176
           PP ALP I  T      H +  K +L   G        P P     SP  D+  T SF
Sbjct: 405 PPAALPPIAYTSGRGKIHHSLPKGALPKEGPPPPPRRLPSPATPPQSPLRDLGRTPSF 462



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>DSBE_CHRVI (Q46476) Thiol:disulfide interchange protein dsbE (Cytochrome c|
           biogenesis protein ccmG)
          Length = 165

 Score = 28.1 bits (61), Expect = 6.1
 Identities = 20/78 (25%), Positives = 31/78 (39%), Gaps = 3/78 (3%)
 Frame = -2

Query: 247 LALYSLXAGPEXIRERVQDLVINRKLLVEAMSP---LGEDAVKGGXGAIYLWAKLPDNCK 77
           L  Y L   P  I   + D       L +   P   L  D + G    + +WA    +C+
Sbjct: 20  LLFYGLQLDPRKIPSPLVDKPAPEFSLPDLKDPNQTLTRDILIGQVSLVNVWASWCPSCR 79

Query: 76  DDFEVVRWLATKHGVAVI 23
            +   +  +A +HGV VI
Sbjct: 80  QEHAELMRIAREHGVRVI 97



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>SYW_CLOAB (Q97LD6) Tryptophanyl-tRNA synthetase (EC 6.1.1.2)|
           (Tryptophan--tRNA ligase) (TrpRS)
          Length = 335

 Score = 28.1 bits (61), Expect = 6.1
 Identities = 14/52 (26%), Positives = 25/52 (48%)
 Frame = -2

Query: 181 NRKLLVEAMSPLGEDAVKGGXGAIYLWAKLPDNCKDDFEVVRWLATKHGVAV 26
           N+K++   + P GE  +    GAI  W KL    +D+++    +   H + V
Sbjct: 4   NKKVIFSGIQPSGELTIGNYFGAIKNWVKL----QDEYDCYYCIVDLHAITV 51



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>WNK4_MOUSE (Q80UE6) Serine/threonine-protein kinase WNK4 (EC 2.7.11.1) (Protein|
            kinase with no lysine 4) (Protein kinase,
            lysine-deficient 4)
          Length = 1222

 Score = 27.7 bits (60), Expect = 8.0
 Identities = 25/84 (29%), Positives = 34/84 (40%)
 Frame = +3

Query: 6    PLALPGITATPCLVASHLTTSKSSLQLSGSLAQR*MAPXPPLTASSPRGDIASTSSFRFI 185
            P +LP  TA P L  +    S  SL +  ++AQ  ++P P L + SP        S    
Sbjct: 894  PPSLPSTTAAPLLSLA----SAFSLAVM-TVAQSLLSPSPGLLSQSPPAPPGPLPSLPLS 948

Query: 186  TRSCTLSLXHSGPAXNEYRARRCP 257
              SC      +  A  E  A R P
Sbjct: 949  LASCDQESLSAQTAETENEASRNP 972



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>TENS1_HUMAN (Q9HBL0) Tensin-1|
          Length = 1735

 Score = 27.7 bits (60), Expect = 8.0
 Identities = 16/41 (39%), Positives = 20/41 (48%), Gaps = 6/41 (14%)
 Frame = +3

Query: 3   PPLALPGITATPCLVASHLTTSKS------SLQLSGSLAQR 107
           PP +LPG+TA P L     T+  S       L L G +A R
Sbjct: 835 PPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHR 875



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>AGI_HORVU (P15312) Root-specific lectin precursor|
          Length = 212

 Score = 27.7 bits (60), Expect = 8.0
 Identities = 15/35 (42%), Positives = 16/35 (45%), Gaps = 1/35 (2%)
 Frame = -1

Query: 200 GARSRDKP-EAACGGNVSPWRGCCQGWXGCHLPLG 99
           GA S DKP   A GG V     CC  W  C +  G
Sbjct: 150 GACSTDKPCGKAAGGKVCTNNYCCSKWGSCGIGPG 184



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>NAS35_CAEEL (P98060) Zinc metalloproteinase nas-35 precursor (EC 3.4.24.21)|
           (Nematode astacin 35) (Dumpy protein 31) (Tollish
           protein 2)
          Length = 592

 Score = 27.7 bits (60), Expect = 8.0
 Identities = 15/42 (35%), Positives = 19/42 (45%)
 Frame = -1

Query: 239 VLIXGWPRVXQGKGARSRDKPEAACGGNVSPWRGCCQGWXGC 114
           VL   + R  +G  AR+R  PEA    + SPW  C      C
Sbjct: 468 VLFRSFYRGGKGFEARARAVPEAGNWNSWSPWTACSATCGAC 509



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>HEP_DROME (Q23977) Dual specificity mitogen-activated protein kinase kinase|
           hemipterous (EC 2.7.12.2) (MAPKK)
          Length = 1178

 Score = 27.7 bits (60), Expect = 8.0
 Identities = 22/58 (37%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
 Frame = +3

Query: 3   PPLALPGITATPCLVASHLTTSKSSLQLSG-SLAQR*MAPXPPLTASSPRGDIASTSS 173
           PPLA     AT    A  L     S+  SG S+AQR   P PP+  ++P G  +++SS
Sbjct: 50  PPLATSASAATSATHAPSL--GAGSVSGSGISIAQR---PAPPVPHATPFGSASASSS 102



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>YFDZ_ECOLI (P77434) Hypothetical aminotransferase yfdZ (EC 2.6.1.-)|
          Length = 412

 Score = 27.7 bits (60), Expect = 8.0
 Identities = 21/85 (24%), Positives = 38/85 (44%), Gaps = 2/85 (2%)
 Frame = -2

Query: 253 QRLALYSLXAGPEXIRERVQDLVINRKLLVEAMSPLGEDAVKGGXGAIYLWAKLPD--NC 80
           Q  A+ +L    + +R+  +     R +LV+ +   G   V+    ++Y+WAK+P+    
Sbjct: 282 QVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGW-MVEMPKASMYVWAKIPEPYAA 340

Query: 79  KDDFEVVRWLATKHGVAVIPGSASG 5
               E  + L  +  V V PG   G
Sbjct: 341 MGSLEFAKKLLNEAKVCVSPGIGFG 365



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>PALY_MALDO (P35512) Phenylalanine ammonia-lyase (EC 4.3.1.5) (Fragment)|
          Length = 235

 Score = 27.7 bits (60), Expect = 8.0
 Identities = 11/26 (42%), Positives = 15/26 (57%), Gaps = 1/26 (3%)
 Frame = -1

Query: 176 EAACGGNV-SPWRGCCQGWXGCHLPL 102
           +A C G +  P  GC +GW G  LP+
Sbjct: 209 QAICQGKIIDPILGCLEGWNGAPLPI 234



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>AAT_AQUAE (O67781) Aspartate aminotransferase (EC 2.6.1.1) (Transaminase A)|
           (ASPAT)
          Length = 394

 Score = 27.7 bits (60), Expect = 8.0
 Identities = 22/62 (35%), Positives = 32/62 (51%), Gaps = 4/62 (6%)
 Frame = -2

Query: 178 RKLLVEAMSPL-GEDAVKGGXGAIYLW---AKLPDNCKDDFEVVRWLATKHGVAVIPGSA 11
           R   VE +S + G D VK   GA Y++   +   +    D ++  +L  K  VAV+PGSA
Sbjct: 304 RDTAVEELSKIPGMDVVKP-EGAFYIFPDFSAYAEKLGGDVKLSEFLLEKAKVAVVPGSA 362

Query: 10  SG 5
            G
Sbjct: 363 FG 364


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,245,174
Number of Sequences: 219361
Number of extensions: 701827
Number of successful extensions: 2203
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 2156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2203
length of database: 80,573,946
effective HSP length: 64
effective length of database: 66,534,842
effective search space used: 1596836208
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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