ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd16b10
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1DFRA_ARATH (P51102) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 52 2e-06
2DFRA_MAIZE (P51108) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 52 2e-06
3DFRA_DIACA (P51104) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 51 4e-06
4DFRA_HORVU (P51106) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 49 2e-05
5DFRA_PETHY (P14720) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 49 2e-05
6DFRA_GERHY (P51105) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 48 2e-05
7DFRA_VITVI (P51110) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 47 4e-05
8DFRA_CALCH (P51103) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 46 9e-05
9DFRA_LYCES (P51107) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 44 6e-04
10YGD9_YEAST (P53183) Hypothetical protein YGL039W 42 0.001
11DFRA_ANTMA (P14721) Dihydroflavonol-4-reductase (EC 1.1.1.219) (... 42 0.002
12YGP7_YEAST (P53111) Hypothetical protein YGL157W 38 0.033
13GRE2_YEAST (Q12068) NADPH-dependent methylglyoxal reductase GRE2... 37 0.056
14BAN_ARATH (Q9SEV0) Leucoanthocyanidin reductase (EC 1.3.1.77) (L... 37 0.073
15SFH1_YEAST (Q06168) Chromatin structure remodeling complex prote... 33 0.80
16PPDK_FLATR (P22221) Pyruvate, phosphate dikinase, chloroplast pr... 32 1.4
17PPDK_FLABI (Q39735) Pyruvate, phosphate dikinase, chloroplast pr... 32 1.4
18ESO1_SCHPO (O42917) N-acetyltransferase eso1 (EC 2.3.1.-) (Siste... 31 3.1
19EDG7_HUMAN (Q9UBY5) Lysophosphatidic acid receptor Edg-7 (LPA re... 30 6.8
20SLY1_DROME (Q24179) Sly1 protein homolog 30 8.9
21PPDK_FLABR (Q39734) Pyruvate, phosphate dikinase, chloroplast pr... 30 8.9
22Y1650_METJA (Q59044) Hypothetical protein MJ1650 30 8.9

>DFRA_ARATH (P51102) Dihydroflavonol-4-reductase (EC 1.1.1.219) (DFR)|
           (Dihydrokaempferol 4-reductase) (TRANSPARENT TESTA 3
           protein)
          Length = 382

 Score = 52.0 bits (123), Expect = 2e-06
 Identities = 31/99 (31%), Positives = 53/99 (53%), Gaps = 1/99 (1%)
 Frame = -2

Query: 588 YVDVRDVARAHILVYERPDARGRYLCICDVLHRAHFLQLLRDLFPYYPITDKCEDDGKPM 409
           YV + D+  AHI +YE+  A+GRY+C           + LR  +P Y +    E   + +
Sbjct: 228 YVHLDDLCNAHIFLYEQAAAKGRYICSSHDATILTISKFLRPKYPEYNVPSTFEGVDENL 287

Query: 408 VKPYRFSNQRLRDLGLDFT-PLKESLHETVLSLQQQGHL 295
            K   FS+++L D+G +F   L+E   E++ + +Q+G L
Sbjct: 288 -KSIEFSSKKLTDMGFNFKYSLEEMFIESIETCRQKGFL 325



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>DFRA_MAIZE (P51108) Dihydroflavonol-4-reductase (EC 1.1.1.219) (DFR)|
           (Dihydrokaempferol 4-reductase)
          Length = 357

 Score = 51.6 bits (122), Expect = 2e-06
 Identities = 31/109 (28%), Positives = 56/109 (51%), Gaps = 5/109 (4%)
 Frame = -2

Query: 585 VDVRDVARAHILVYERPDARGRYLCICDVLHRAHFLQLLRDLFPYYPITDK---CEDDGK 415
           + + D+  A I ++E P A GRY+C    +       +LRD +P Y +  +    +DD  
Sbjct: 234 IHLDDLCDAEIFLFENPAAAGRYVCSSHDVTIHGLAAMLRDRYPEYDVPQRFPGIQDD-- 291

Query: 414 PMVKPYRFSNQRLRDLGLDF--TPLKESLHETVLSLQQQGHLDLPVVPG 274
             ++P RFS+++L+DLG  F    L++     + + Q++G + L    G
Sbjct: 292 --LQPVRFSSKKLQDLGFTFRYKTLEDMFDAAIRTCQEKGLIPLATAAG 338



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>DFRA_DIACA (P51104) Dihydroflavonol-4-reductase (EC 1.1.1.219) (DFR)|
           (Dihydrokaempferol 4-reductase)
          Length = 360

 Score = 50.8 bits (120), Expect = 4e-06
 Identities = 32/101 (31%), Positives = 54/101 (53%), Gaps = 1/101 (0%)
 Frame = -2

Query: 588 YVDVRDVARAHILVYERPDARGRYLCICDVLHRAHFLQLLRDLFPYYPITDKCEDDGKPM 409
           +V + D+  +HI +YE P A GRY+            ++LR+ +P Y +  K +D  + M
Sbjct: 244 FVHLDDLCMSHIFLYENPKANGRYIASACAATIYDIAKMLREEYPEYNVPTKFKDYKEDM 303

Query: 408 VKPYRFSNQRLRDLGLDFT-PLKESLHETVLSLQQQGHLDL 289
            +  +FS+++L DLG +F   LK+     V S + +G L L
Sbjct: 304 GQ-VQFSSKKLTDLGFEFKYGLKDMYTAAVESCRAKGLLPL 343



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>DFRA_HORVU (P51106) Dihydroflavonol-4-reductase (EC 1.1.1.219) (DFR)|
           (Dihydrokaempferol 4-reductase)
          Length = 354

 Score = 48.5 bits (114), Expect = 2e-05
 Identities = 32/108 (29%), Positives = 53/108 (49%), Gaps = 3/108 (2%)
 Frame = -2

Query: 585 VDVRDVARAHILVYERPDARGRYLCICDVLHRAHFLQLLRDLFPYYPITDKCE--DDGKP 412
           V + D+  A   ++E P+A GRY+C           ++L+D FP Y I  K    DD   
Sbjct: 229 VHLDDLCDAMTFLFEHPEANGRYICSSHDATIHGLARMLQDRFPEYDIPQKFAGVDDN-- 286

Query: 411 MVKPYRFSNQRLRDLGLDFTPLKESLHETVL-SLQQQGHLDLPVVPGP 271
            ++P  FS+++L D G  F    E + +  + + + +G + L  VP P
Sbjct: 287 -LQPIHFSSKKLLDHGFSFRYTTEDMFDAAIHTCRDKGLIPLGDVPAP 333



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>DFRA_PETHY (P14720) Dihydroflavonol-4-reductase (EC 1.1.1.219) (DFR)|
           (Dihydrokaempferol 4-reductase)
          Length = 380

 Score = 48.5 bits (114), Expect = 2e-05
 Identities = 30/100 (30%), Positives = 50/100 (50%), Gaps = 2/100 (2%)
 Frame = -2

Query: 588 YVDVRDVARAHILVYERPDARGRYLCICDVLHRAHFLQLLRDLFP--YYPITDKCEDDGK 415
           YV + D+  AHI +YE P A GR++C           +++R+ +P  Y P   K  D   
Sbjct: 238 YVHLDDLCEAHIFLYEHPKADGRFICSSHHAIIYDVAKMVREKWPEYYVPTEFKGIDKDL 297

Query: 414 PMVKPYRFSNQRLRDLGLDFTPLKESLHETVLSLQQQGHL 295
           P+V    FS+++L D+G  F    E +++  +   +Q  L
Sbjct: 298 PVVS---FSSKKLTDMGFQFKYTLEDMYKGAIDTCRQKQL 334



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>DFRA_GERHY (P51105) Dihydroflavonol-4-reductase (EC 1.1.1.219) (DFR)|
           (Dihydrokaempferol 4-reductase)
          Length = 366

 Score = 48.1 bits (113), Expect = 2e-05
 Identities = 27/80 (33%), Positives = 43/80 (53%), Gaps = 2/80 (2%)
 Frame = -2

Query: 588 YVDVRDVARAHILVYERPDARGRYLCICDVLHRAHFLQLLRDLFPYYPITDKCE--DDGK 415
           YV + D+   HI +YE P A+GRY+C           ++++D +P Y I  K    D+  
Sbjct: 229 YVHLDDLCECHIYLYENPKAKGRYICSSHDATIHQLAKIIKDKWPEYYIPTKFPGIDEEL 288

Query: 414 PMVKPYRFSNQRLRDLGLDF 355
           P+V    FS+++L D G +F
Sbjct: 289 PIVS---FSSKKLIDTGFEF 305



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>DFRA_VITVI (P51110) Dihydroflavonol-4-reductase (EC 1.1.1.219) (DFR)|
           (Dihydrokaempferol 4-reductase)
          Length = 337

 Score = 47.4 bits (111), Expect = 4e-05
 Identities = 30/83 (36%), Positives = 47/83 (56%), Gaps = 5/83 (6%)
 Frame = -2

Query: 588 YVDVRDVARAHILVYERPDARGRYLCI---CDVLHRAHFLQLLRDLFPYY--PITDKCED 424
           +V + D+  AHI ++E P A GRY+C    C +L  A   ++LR+ +P Y  P   K  D
Sbjct: 228 FVHLDDLCNAHIYLFENPKAEGRYICSSHDCIILDLA---KMLREKYPEYNIPTEFKGVD 284

Query: 423 DGKPMVKPYRFSNQRLRDLGLDF 355
           +    +K   FS+++L DLG +F
Sbjct: 285 EN---LKSVCFSSKKLTDLGFEF 304



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>DFRA_CALCH (P51103) Dihydroflavonol-4-reductase (EC 1.1.1.219) (DFR)|
           (Dihydrokaempferol 4-reductase)
          Length = 364

 Score = 46.2 bits (108), Expect = 9e-05
 Identities = 28/101 (27%), Positives = 52/101 (51%), Gaps = 3/101 (2%)
 Frame = -2

Query: 588 YVDVRDVARAHILVYERPDARGRYLCICDVLHRAHFLQLLRDLFPYYPITDKCE--DDGK 415
           YV + D+   HI +YE P+A+GRY+C           ++++  +P Y +  +    D+  
Sbjct: 229 YVHLDDLCECHIFLYENPEAKGRYICSKQDATIHQLARMIKQKWPEYHVPTQFAGIDEEL 288

Query: 414 PMVKPYRFSNQRLRDLGLDFT-PLKESLHETVLSLQQQGHL 295
           P V    FS+++L D+G  F   L++     + S +++G L
Sbjct: 289 PTVS---FSSKKLIDMGFKFKYDLEDMFKGAIDSCKEKGFL 326



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>DFRA_LYCES (P51107) Dihydroflavonol-4-reductase (EC 1.1.1.219) (DFR)|
           (Dihydrokaempferol 4-reductase)
          Length = 379

 Score = 43.5 bits (101), Expect = 6e-04
 Identities = 25/98 (25%), Positives = 47/98 (47%)
 Frame = -2

Query: 588 YVDVRDVARAHILVYERPDARGRYLCICDVLHRAHFLQLLRDLFPYYPITDKCEDDGKPM 409
           YV + D+  AHI +YE P A GR++C           +++R  +P Y +  + +   K +
Sbjct: 237 YVHLDDLCEAHIFLYEHPKAEGRFICSSHHAIIYDVAKMVRQKWPEYYVPTEFKGIDKDL 296

Query: 408 VKPYRFSNQRLRDLGLDFTPLKESLHETVLSLQQQGHL 295
                FS+++L D+   F    E +++  +   +Q  L
Sbjct: 297 AL-VSFSSKKLMDIKFQFKHTLEDMYKGAIETCRQKQL 333



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>YGD9_YEAST (P53183) Hypothetical protein YGL039W|
          Length = 348

 Score = 42.4 bits (98), Expect = 0.001
 Identities = 18/46 (39%), Positives = 31/46 (67%), Gaps = 1/46 (2%)
 Frame = -2

Query: 588 YVDVRDVARAHILVYERPDARGRYLCIC-DVLHRAHFLQLLRDLFP 454
           ++DVRDV++AH+L +E+P+  G+ L +C D+      L +L + FP
Sbjct: 244 FIDVRDVSKAHLLAFEKPECAGQRLFLCEDMFCSQEALDILNEEFP 289



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>DFRA_ANTMA (P14721) Dihydroflavonol-4-reductase (EC 1.1.1.219) (DFR)|
           (Dihydrokaempferol 4-reductase)
          Length = 446

 Score = 42.0 bits (97), Expect = 0.002
 Identities = 28/101 (27%), Positives = 52/101 (51%), Gaps = 3/101 (2%)
 Frame = -2

Query: 588 YVDVRDVARAHILVYERPDARGRYLCICDVLHRAHFLQLLRDLFPYYPITDKCE--DDGK 415
           YV + D+   HI ++E P A GRY+C           +L+ + +P Y I D+ E  D   
Sbjct: 240 YVHLDDLCEGHIFLFEYPKAEGRYICSSHDATIYDIAKLITENWPEYHIPDEFEGIDKDI 299

Query: 414 PMVKPYRFSNQRLRDLGLDFT-PLKESLHETVLSLQQQGHL 295
           P+V    FS++++  +G  F   L++ +   + + +++G L
Sbjct: 300 PVVS---FSSKKMIGMGFIFKYTLEDMVRGAIDTCREKGML 337



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>YGP7_YEAST (P53111) Hypothetical protein YGL157W|
          Length = 347

 Score = 37.7 bits (86), Expect = 0.033
 Identities = 28/106 (26%), Positives = 51/106 (48%), Gaps = 9/106 (8%)
 Frame = -2

Query: 603 NVVTAYVDVRDVARAHILVYERPDARGRYLCICDVLHRA-HFLQLLRDLFPYYPITDKCE 427
           N    ++DVRDV++AH++  E+P+  G+ L + + L      + +L + FP   +  K  
Sbjct: 238 NYCGPFIDVRDVSKAHLVAIEKPECTGQRLVLSEGLFCCQEIVDILNEEFP--QLKGKIA 295

Query: 426 DDGKPMVKP-------YRFSNQRLRD-LGLDFTPLKESLHETVLSL 313
             G+P   P        +F N + +  LG  F  LK+ + +T   +
Sbjct: 296 -TGEPATGPSFLEKNSCKFDNSKTKKLLGFQFYNLKDCIVDTAAQM 340



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>GRE2_YEAST (Q12068) NADPH-dependent methylglyoxal reductase GRE2 (EC|
           1.1.1.283) (Genes de respuesta a estres protein 2)
          Length = 342

 Score = 37.0 bits (84), Expect = 0.056
 Identities = 31/120 (25%), Positives = 56/120 (46%), Gaps = 7/120 (5%)
 Frame = -2

Query: 663 VVPSTVHISRYLTGAKKTCPNVVTAYVDVRDVARAHILVYERPDARGRYLCICDVLHRAH 484
           +V S +H+S      +   P +   Y+DVRDVA+AH++ +++ +  G+ L + +   R  
Sbjct: 219 LVNSLMHLS-----PEDKIPELFGGYIDVRDVAKAHLVAFQKRETIGQRLIVSEA--RFT 271

Query: 483 FLQLLRDLFPYYPITDKCEDDGKP------MVKPYRFSNQRLRD-LGLDFTPLKESLHET 325
              +L  L   +P+       GKP              N++ +  LG  F  LKE++ +T
Sbjct: 272 MQDVLDILNEDFPVLKGNIPVGKPGSGATHNTLGATLDNKKSKKLLGFKFRNLKETIDDT 331



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>BAN_ARATH (Q9SEV0) Leucoanthocyanidin reductase (EC 1.3.1.77) (LAR) (Protein|
           BANYULS) (Anthocyanin spotted testa) (ast)
          Length = 340

 Score = 36.6 bits (83), Expect = 0.073
 Identities = 22/79 (27%), Positives = 37/79 (46%)
 Frame = -2

Query: 591 AYVDVRDVARAHILVYERPDARGRYLCICDVLHRAHFLQLLRDLFPYYPITDKCEDDGKP 412
           ++V V D+ARAH+ + E+  A GRY+C             L   +P Y +  + E+    
Sbjct: 240 SFVHVDDLARAHLFLAEKETASGRYICCAYNTSVPEIADFLIQRYPKYNVLSEFEEG--L 297

Query: 411 MVKPYRFSNQRLRDLGLDF 355
            +     S+Q+L + G  F
Sbjct: 298 SIPKLTLSSQKLINEGFRF 316



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>SFH1_YEAST (Q06168) Chromatin structure remodeling complex protein SFH1 (SNF5|
           homolog 1)
          Length = 426

 Score = 33.1 bits (74), Expect = 0.80
 Identities = 21/48 (43%), Positives = 30/48 (62%), Gaps = 6/48 (12%)
 Frame = -2

Query: 402 PYRFSNQRLRDLGL--DFTPL-KESLHETVLSLQQ---QGHLDLPVVP 277
           P RF+   ++DLGL  +F PL  +SLHET+L +++    GHL    VP
Sbjct: 307 PERFATSIVQDLGLTREFIPLISQSLHETILKIKKDWVDGHLIQDHVP 354



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>PPDK_FLATR (P22221) Pyruvate, phosphate dikinase, chloroplast precursor (EC|
           2.7.9.1) (Pyruvate, orthophosphate dikinase)
          Length = 953

 Score = 32.3 bits (72), Expect = 1.4
 Identities = 28/84 (33%), Positives = 42/84 (50%), Gaps = 1/84 (1%)
 Frame = +1

Query: 289 QIQVTLLLQG*HRLVQTLLQWSEVQPQVPEPLVGEPVWLDHRLAVILTLVGNGIVWEEVS 468
           ++QV  + Q     V    Q   V P++  PLVG P  L H+++VI  +  N  V+ E+ 
Sbjct: 761 EMQVRAIFQA---AVSMTNQGVTVIPEIMVPLVGTPQELRHQISVIRGVAAN--VFAEMG 815

Query: 469 KQLE-KVGAVQHVADAEVSAACIG 537
             LE KVG +  +  A + A  IG
Sbjct: 816 VTLEYKVGTMIEIPRAALIAEEIG 839



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>PPDK_FLABI (Q39735) Pyruvate, phosphate dikinase, chloroplast precursor (EC|
           2.7.9.1) (Pyruvate, orthophosphate dikinase)
           (Cold-sensitive pyruvate, orthophosphate dikinase)
          Length = 953

 Score = 32.3 bits (72), Expect = 1.4
 Identities = 28/84 (33%), Positives = 42/84 (50%), Gaps = 1/84 (1%)
 Frame = +1

Query: 289 QIQVTLLLQG*HRLVQTLLQWSEVQPQVPEPLVGEPVWLDHRLAVILTLVGNGIVWEEVS 468
           ++QV  + Q     V    Q   V P++  PLVG P  L H+++VI  +  N  V+ E+ 
Sbjct: 761 EMQVRAIFQA---AVSMTNQGVTVIPEIMVPLVGTPQELRHQISVIRGVAAN--VFAEMG 815

Query: 469 KQLE-KVGAVQHVADAEVSAACIG 537
             LE KVG +  +  A + A  IG
Sbjct: 816 VTLEYKVGTMIEIPRAALIAEEIG 839



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>ESO1_SCHPO (O42917) N-acetyltransferase eso1 (EC 2.3.1.-) (Sister chromatid|
           cohesion protein eso1) (ECO1 homolog)
          Length = 872

 Score = 31.2 bits (69), Expect = 3.1
 Identities = 20/77 (25%), Positives = 36/77 (46%), Gaps = 2/77 (2%)
 Frame = -2

Query: 645 HISRY--LTGAKKTCPNVVTAYVDVRDVARAHILVYERPDARGRYLCICDVLHRAHFLQL 472
           +ISR+  +T AKK CP + TA+V       +    +E P+      C+    H +  +++
Sbjct: 73  NISRHETVTEAKKKCPELCTAHVKTWKAGESEAKYHENPNPNYYKTCLDPYRHES--VKI 130

Query: 471 LRDLFPYYPITDKCEDD 421
           L  +  + P+  K   D
Sbjct: 131 LNIIKKHAPVVKKASID 147



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>EDG7_HUMAN (Q9UBY5) Lysophosphatidic acid receptor Edg-7 (LPA receptor 3)|
           (LPA-3)
          Length = 353

 Score = 30.0 bits (66), Expect = 6.8
 Identities = 13/26 (50%), Positives = 17/26 (65%)
 Frame = -2

Query: 192 LHWWNINNLCPFTIMIQEYLISYVYV 115
           L +W ++NL  F IM+  YL  YVYV
Sbjct: 188 LVFWTVSNLMAFLIMVVVYLRIYVYV 213



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>SLY1_DROME (Q24179) Sly1 protein homolog|
          Length = 657

 Score = 29.6 bits (65), Expect = 8.9
 Identities = 19/61 (31%), Positives = 29/61 (47%), Gaps = 1/61 (1%)
 Frame = +1

Query: 325 RLVQTLLQWSEVQPQVPEPLVGEPVWLDHRLAVILTLVGNGIVWEEVS-KQLEKVGAVQH 501
           R +  + Q   +  Q P+ L  EPVW      +I   VG  I+   +S K+L ++G   H
Sbjct: 7   RQINAIKQMLNLNSQQPKALAAEPVW----KILIYDRVGQDIISPIISIKELRELGVTLH 62

Query: 502 V 504
           V
Sbjct: 63  V 63



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>PPDK_FLABR (Q39734) Pyruvate, phosphate dikinase, chloroplast precursor (EC|
           2.7.9.1) (Pyruvate, orthophosphate dikinase)
           (Cold-sensitive pyruvate, orthophosphate dikinase)
          Length = 955

 Score = 29.6 bits (65), Expect = 8.9
 Identities = 21/57 (36%), Positives = 31/57 (54%), Gaps = 1/57 (1%)
 Frame = +1

Query: 358 VQPQVPEPLVGEPVWLDHRLAVILTLVGNGIVWEEVSKQLE-KVGAVQHVADAEVSA 525
           V P++  PLVG P  L H++ VI  +  N  V+ E+   LE KVG +  +  A + A
Sbjct: 783 VIPEIMVPLVGTPQELRHQIGVIRGVAAN--VFAEMGLTLEYKVGTMIEIPRAALIA 837



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>Y1650_METJA (Q59044) Hypothetical protein MJ1650|
          Length = 384

 Score = 29.6 bits (65), Expect = 8.9
 Identities = 14/57 (24%), Positives = 28/57 (49%)
 Frame = +1

Query: 358 VQPQVPEPLVGEPVWLDHRLAVILTLVGNGIVWEEVSKQLEKVGAVQHVADAEVSAA 528
           V  +VPE  VG+ V    +  ++  +  + +  + +  + EK+G   H+A+ E   A
Sbjct: 262 VSVRVPEYKVGDVVEYKDKYYLVTAITEDKVYMKSIDYKREKIGLAWHIAEKETKMA 318


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,097,860
Number of Sequences: 219361
Number of extensions: 1822892
Number of successful extensions: 4996
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 4771
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4985
length of database: 80,573,946
effective HSP length: 108
effective length of database: 56,882,958
effective search space used: 6712189044
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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