ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | rbasd15m08 |
|---|---|
| Clone Library Name | barley_pub |
>SMT1_ORYSA (Q6ZIX2) Cycloartenol-C-24-methyltransferase 1 (EC 2.1.1.41)| (24-sterol C-methyltransferase 1) (Sterol C-methyltransferase 1) Length = 344 Score = 211 bits (537), Expect = 2e-54 Identities = 101/112 (90%), Positives = 109/112 (97%) Frame = -2 Query: 682 PDIRSTRQCLQAVKDAGFEVIWDKDLAEDSPLPWYLPLDPSRFSLSSFRLTTMGRIITRN 503 PDIRST+QCLQA KDAGFEVIWDKDLAEDSP+PWYLPLDPSRFSLSSFRLTT+GR ITR Sbjct: 233 PDIRSTQQCLQAAKDAGFEVIWDKDLAEDSPVPWYLPLDPSRFSLSSFRLTTVGRAITRT 292 Query: 502 MVKALEYVGLAPEGSQRVSSFLEKAAEGLVEGGKKEIFTPMYFFVVRKPLSE 347 MVKALEYVGLAP+GS+RVS+FLEKAAEGLVEGGKKEIFTPMYFF+VRKP+SE Sbjct: 293 MVKALEYVGLAPQGSERVSNFLEKAAEGLVEGGKKEIFTPMYFFLVRKPISE 344
>SMT1_ARATH (Q9LM02) Cycloartenol-C-24-methyltransferase (EC 2.1.1.41)| (24-sterol C-methyltransferase 1) (Sterol C-methyltransferase 1) (STEROL METHYLTRANSFERASE 1) (CEPHALOPOD protein) Length = 336 Score = 179 bits (453), Expect = 9e-45 Identities = 82/109 (75%), Positives = 99/109 (90%) Frame = -2 Query: 682 PDIRSTRQCLQAVKDAGFEVIWDKDLAEDSPLPWYLPLDPSRFSLSSFRLTTMGRIITRN 503 PDIR T +CL+A+K AGFEVIW+KDLA+DSP+PWYLPLD + FSLSSFRLT +GR IT+N Sbjct: 227 PDIRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKN 286 Query: 502 MVKALEYVGLAPEGSQRVSSFLEKAAEGLVEGGKKEIFTPMYFFVVRKP 356 MVK LEY+ LAP+GSQRVS+FLE+AAEGLV+GG++EIFTPMYFF+ RKP Sbjct: 287 MVKILEYIRLAPQGSQRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKP 335
>ERG6_CANAL (O74198) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 376 Score = 98.6 bits (244), Expect = 2e-20 Identities = 54/120 (45%), Positives = 78/120 (65%), Gaps = 8/120 (6%) Frame = -2 Query: 682 PDIRSTRQCLQAVKDAGFEVIWDKDLAE-DSPLPWYLPLDPS-RFS------LSSFRLTT 527 P + S + QA+K+ GFE+ + KDLA+ D +PWY PL +F L+ FR + Sbjct: 255 PKMYSRKVAEQALKNVGFEIEYQKDLADVDDEIPWYYPLSGDLKFCQTFGDYLTVFRTSR 314 Query: 526 MGRIITRNMVKALEYVGLAPEGSQRVSSFLEKAAEGLVEGGKKEIFTPMYFFVVRKPLSE 347 +GR IT V +E +GLAP+GS++V+ LE AA LVEGG++++FTPM +VVRKPL + Sbjct: 315 IGRFITTESVGLMEKIGLAPKGSKQVTHALEDAAVNLVEGGRQKLFTPMMLYVVRKPLEK 374
>ERG6_CANGA (Q6FRZ7) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 372 Score = 95.1 bits (235), Expect = 2e-19 Identities = 53/107 (49%), Positives = 68/107 (63%), Gaps = 8/107 (7%) Frame = -2 Query: 652 QAVKDAGFEVIWDKDLA-EDSPLPWYLPLDPSRFSLSS-------FRLTTMGRIITRNMV 497 QA+K+ GFEV KDLA ED +PWY PL + S FR + GR T MV Sbjct: 262 QALKNVGFEVEVSKDLADEDDEIPWYYPLTGEWKYVQSLSDIATFFRTSYFGRKFTNCMV 321 Query: 496 KALEYVGLAPEGSQRVSSFLEKAAEGLVEGGKKEIFTPMYFFVVRKP 356 +E VG+AP+GS V++ LE AA GLVEGG+K++FTPM+ FV +KP Sbjct: 322 SIMEKVGVAPQGSVEVTAALENAAVGLVEGGRKKLFTPMFLFVAKKP 368
>ERG6_YARLI (Q6C2D9) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 381 Score = 92.0 bits (227), Expect = 1e-18 Identities = 48/117 (41%), Positives = 75/117 (64%), Gaps = 8/117 (6%) Frame = -2 Query: 682 PDIRSTRQCLQAVKDAGFEVIWDKDLA-EDSPLPWYLPLDPSRFSLSSF-------RLTT 527 P + ++A+++ GF++ + +DLA +D+ +PWY PL + S R + Sbjct: 261 PKMYKREVAVKALENVGFDIEYQEDLAADDAEVPWYYPLAGEWKYVQSLNDIVTIGRTSR 320 Query: 526 MGRIITRNMVKALEYVGLAPEGSQRVSSFLEKAAEGLVEGGKKEIFTPMYFFVVRKP 356 +GR++T N++ ALE +GLAP+GS++V+ LE AA LV GGKK++FTPM FV RKP Sbjct: 321 LGRMVTMNVIGALEKIGLAPQGSRQVTEALEDAAVNLVAGGKKKLFTPMMLFVSRKP 377
>ERG6_YEAST (P25087) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 382 Score = 91.3 bits (225), Expect = 3e-18 Identities = 50/107 (46%), Positives = 68/107 (63%), Gaps = 8/107 (7%) Frame = -2 Query: 652 QAVKDAGFEVIWDKDLAE-DSPLPWYLPLDPSRFSLSS-------FRLTTMGRIITRNMV 497 +A+K+ GFEV+ +DLA+ D +PWY PL + + FR + +GR T MV Sbjct: 261 KALKNCGFEVLVSEDLADNDDEIPWYYPLTGEWKYVQNLANLATFFRTSYLGRQFTTAMV 320 Query: 496 KALEYVGLAPEGSQRVSSFLEKAAEGLVEGGKKEIFTPMYFFVVRKP 356 +E +GLAPEGS+ V++ LE AA GLV GGK ++FTPM FV RKP Sbjct: 321 TVMEKLGLAPEGSKEVTAALENAAVGLVAGGKSKLFTPMMLFVARKP 367
>ERG6_CLALS (Q875K1) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 375 Score = 89.7 bits (221), Expect = 7e-18 Identities = 45/108 (41%), Positives = 71/108 (65%), Gaps = 8/108 (7%) Frame = -2 Query: 652 QAVKDAGFEVIWDKDLAE-DSPLPWYLPL-------DPSRFSLSSFRLTTMGRIITRNMV 497 QA+K+ GF++ ++KDLA+ + +PWY PL + FR + +GR +T N+V Sbjct: 265 QALKNVGFDIEYEKDLADVNDEIPWYYPLAGEWKYVQTLSDCFTIFRTSKIGRTVTTNVV 324 Query: 496 KALEYVGLAPEGSQRVSSFLEKAAEGLVEGGKKEIFTPMYFFVVRKPL 353 +E +GLAP+GS++V+ LE AA LV GG++++FTPM ++ RKPL Sbjct: 325 GLMEKIGLAPKGSKQVTGALEDAAVNLVAGGEQKLFTPMMLYIARKPL 372
>ERG6_KLULA (Q6CYB3) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 371 Score = 89.4 bits (220), Expect = 1e-17 Identities = 49/117 (41%), Positives = 70/117 (59%), Gaps = 8/117 (6%) Frame = -2 Query: 682 PDIRSTRQCLQAVKDAGFEVIWDKDLAE-DSPLPWYLPLDPSRFSLSS-------FRLTT 527 P + S A+ GFE++ + DLA+ D +PWY PL ++S FR + Sbjct: 252 PKMYSVDAARDALSKVGFEILEENDLADNDDEIPWYAPLTGEWKYVNSVADLATFFRTSR 311 Query: 526 MGRIITRNMVKALEYVGLAPEGSQRVSSFLEKAAEGLVEGGKKEIFTPMYFFVVRKP 356 +GR T +MV E +GLAP+GS V++ LE+AA GLV GG+K++FTPM FV +KP Sbjct: 312 LGRAFTTSMVTVFEKLGLAPKGSVSVTNALEEAAVGLVAGGEKKLFTPMMLFVAKKP 368
>ERG6_PNECA (Q96WX4) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 377 Score = 88.6 bits (218), Expect = 2e-17 Identities = 48/118 (40%), Positives = 73/118 (61%), Gaps = 8/118 (6%) Frame = -2 Query: 682 PDIRSTRQCLQAVKDAGFEVIWDKDLA-EDSPLPWYLPLDPSRFSLSSFR-------LTT 527 P I + A+ GFE+I ++L+ ++SPLPWY LD + SFR +TT Sbjct: 259 PKISKIGEAEAALIKVGFEIIHSEELSTKNSPLPWYYYLDGDLRKVRSFRDFISIARMTT 318 Query: 526 MGRIITRNMVKALEYVGLAPEGSQRVSSFLEKAAEGLVEGGKKEIFTPMYFFVVRKPL 353 +G+ + + + +E++GL P+GS++V+ L AA+ LV+ GKKEIFTPM +V RKPL Sbjct: 319 IGKWLISSFIGLMEFIGLLPKGSKKVNDILLVAADSLVKAGKKEIFTPMQLWVCRKPL 376
>ERG6_DEBHA (Q6BRB7) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 377 Score = 87.4 bits (215), Expect = 4e-17 Identities = 46/110 (41%), Positives = 71/110 (64%), Gaps = 8/110 (7%) Frame = -2 Query: 652 QAVKDAGFEVIWDKDLAE-DSPLPWYLPLDPSRFSLSS-------FRLTTMGRIITRNMV 497 +A+K GFE+ ++ DLA+ + +PWY PL + + FR + +GR IT V Sbjct: 266 KALKSVGFEIEYEADLADKEDDIPWYYPLSGEWKYVQTLSDYFTIFRTSKVGRTITTEAV 325 Query: 496 KALEYVGLAPEGSQRVSSFLEKAAEGLVEGGKKEIFTPMYFFVVRKPLSE 347 +E +GLAP+GS++V++ LE AA LVEGG++++FTPM +V RKP +E Sbjct: 326 GLMEKLGLAPKGSKQVTNALEDAAVNLVEGGRQKLFTPMMLYVCRKPANE 375
>ERG6_ASHGO (Q759S7) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 373 Score = 85.5 bits (210), Expect = 1e-16 Identities = 49/117 (41%), Positives = 67/117 (57%), Gaps = 8/117 (6%) Frame = -2 Query: 682 PDIRSTRQCLQAVKDAGFEVIWDKDLAE-DSPLPWYLPLD------PSRFSLSSF-RLTT 527 P + S + A+ GFE++ D+D A+ D P+PWY PL + L +F R + Sbjct: 253 PKMYSVKVARDALAKVGFEILVDEDRADNDDPVPWYYPLTGEWAYIQTIADLGTFVRTSA 312 Query: 526 MGRIITRNMVKALEYVGLAPEGSQRVSSFLEKAAEGLVEGGKKEIFTPMYFFVVRKP 356 +GR +T V +E + LAP GS V + LE AA GLV GGK ++FTPM FV RKP Sbjct: 313 LGRRVTSAFVSLMETLRLAPRGSSEVVNALESAAAGLVAGGKSKLFTPMMLFVARKP 369
>ERG6_NEUCR (Q9P3R1) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 379 Score = 83.6 bits (205), Expect = 5e-16 Identities = 44/110 (40%), Positives = 67/110 (60%), Gaps = 8/110 (7%) Frame = -2 Query: 655 LQAVKDAGFEVIWDKDLAE-DSPLPWYLPLDPSRFSLSSF-------RLTTMGRIITRNM 500 L A+K+ GFE++ +DLA+ + P+PWY P+ + S+ R+T R I Sbjct: 270 LAAMKNVGFELVHHEDLADRNDPMPWYWPIAGELRYMQSYLDFFTVVRMTHTARRILHGF 329 Query: 499 VKALEYVGLAPEGSQRVSSFLEKAAEGLVEGGKKEIFTPMYFFVVRKPLS 350 LE VGLAP+G+++ + L + A+GLV G KK++FTPMY V +KPL+ Sbjct: 330 AGILETVGLAPKGTKKTADALARGADGLVAGAKKKLFTPMYLMVGKKPLN 379
>ERG6_MAGGR (Q5EN22) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 390 Score = 83.2 bits (204), Expect = 7e-16 Identities = 42/120 (35%), Positives = 72/120 (60%), Gaps = 9/120 (7%) Frame = -2 Query: 679 DIRSTRQCLQAVKDAGFEVIWDKDLAEDSP-LPWYLPLDPSRFS--------LSSFRLTT 527 ++++ + + A+K AGFE++ +DLA++S PWY PL + + LS+F +T Sbjct: 270 NLQTAQTAIAAIKAAGFELLESEDLADNSDRAPWYWPLGGNAWQYASTFGDILSTFTMTP 329 Query: 526 MGRIITRNMVKALEYVGLAPEGSQRVSSFLEKAAEGLVEGGKKEIFTPMYFFVVRKPLSE 347 GR I ++ +E +GL P G+++ + L A LV GGK+ +FTPM+ V RKP+++ Sbjct: 330 AGRAIAHTVLGVVESIGLVPPGTKKTADSLSTTAAALVAGGKEGLFTPMFLMVARKPVAK 389
>ERG6_SCHPO (O14321) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 378 Score = 77.8 bits (190), Expect = 3e-14 Identities = 41/114 (35%), Positives = 69/114 (60%), Gaps = 11/114 (9%) Frame = -2 Query: 664 RQC--LQAVKDAGFEVIWDKDLAE-DSP-LPWYLPLDPSRFSLSS-------FRLTTMGR 518 R+C ++A+K GF ++ + DL + D+P LPWY PL + FR + +G+ Sbjct: 261 RKCDAVEAIKKVGFNLLEEDDLTDHDNPDLPWYYPLTGDITKCQNIWDVFTVFRTSRLGK 320 Query: 517 IITRNMVKALEYVGLAPEGSQRVSSFLEKAAEGLVEGGKKEIFTPMYFFVVRKP 356 ++TR V+ LE +G+A +G+ +V L A +GL+EGG+ +FTPM+ + +KP Sbjct: 321 LVTRYSVQFLEKIGVAAKGTSKVGDTLAIAQKGLIEGGETHLFTPMFLMIAKKP 374
>SMT2_ARATH (Q39227) 24-methylenesterol C-methyltransferase 2 (EC 2.1.1.143)| (24-sterol C-methyltransferase 2) (Sterol-C-methyltransferase 2) Length = 361 Score = 61.2 bits (147), Expect = 3e-09 Identities = 36/111 (32%), Positives = 55/111 (49%) Frame = -2 Query: 682 PDIRSTRQCLQAVKDAGFEVIWDKDLAEDSPLPWYLPLDPSRFSLSSFRLTTMGRIITRN 503 P +R+ + K GFE++ +KDLA PW+ L R + + Sbjct: 256 PGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAYWRNHI---------- 305 Query: 502 MVKALEYVGLAPEGSQRVSSFLEKAAEGLVEGGKKEIFTPMYFFVVRKPLS 350 +V+ L VG+AP+G+ V L K A+ L GG+ IF+PM+ + RKP S Sbjct: 306 VVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGETGIFSPMHMILCRKPES 356
>SMT3B_ARATH (Q94JS4) 24-methylenesterol C-methyltransferase 3 (EC 2.1.1.143)| (24-sterol C-methyltransferase 3) (Sterol-C-methyltransferase 3) Length = 359 Score = 60.8 bits (146), Expect = 4e-09 Identities = 41/112 (36%), Positives = 56/112 (50%), Gaps = 3/112 (2%) Frame = -2 Query: 682 PDIRSTRQCLQAVKDAGFEVIWDKDLAEDSPLPWYLPLDPSRFSLSSFRLTTMGRIIT-R 506 P +RS K GFEV+ +KDLA+ PW+ L MGRI R Sbjct: 256 PGLRSYADIAVTAKKVGFEVVKEKDLAKPPSKPWWNRLK-------------MGRIAYWR 302 Query: 505 N--MVKALEYVGLAPEGSQRVSSFLEKAAEGLVEGGKKEIFTPMYFFVVRKP 356 N +V L +G+AP+G+ V L K A+ L GG+ IF+PM+ + RKP Sbjct: 303 NHVVVVILSAIGVAPKGTVDVHKMLFKTADYLTRGGETGIFSPMHMILCRKP 354
>SMT2_ORYSA (O82427) 24-methylenesterol C-methyltransferase 2 (EC 2.1.1.143)| (24-sterol C-methyltransferase 2) (Sterol-C-methyltransferase 2) Length = 363 Score = 58.9 bits (141), Expect = 1e-08 Identities = 39/113 (34%), Positives = 57/113 (50%), Gaps = 3/113 (2%) Frame = -2 Query: 682 PDIRSTRQCLQAVKDAGFEVIWDKDLAEDSPLPWYLPLDPSRFSLSSFRLTTMGRIIT-R 506 P +R + K+ GFEV+ + DLA LPW+ L MGRI R Sbjct: 261 PGLRRQDEIASIAKEVGFEVLKELDLALPPALPWWTRLK-------------MGRIAYWR 307 Query: 505 N--MVKALEYVGLAPEGSQRVSSFLEKAAEGLVEGGKKEIFTPMYFFVVRKPL 353 N +V+ L + +AP+G V L + A+ L GG+ IFTPM+ ++RKP+ Sbjct: 308 NSLVVRVLTMLRIAPKGVCEVHEMLYETAQHLTRGGETGIFTPMHMVLLRKPV 360
>SIPC_SALTY (Q56020) Cell invasion protein sipC (Effector protein sipC)| Length = 409 Score = 31.2 bits (69), Expect = 3.1 Identities = 26/93 (27%), Positives = 44/93 (47%), Gaps = 6/93 (6%) Frame = +3 Query: 381 IGVKISFLPPSTRPSAAFSKKLETLWLPSGARPTYSNALTILRVIIRPMVVNRKLLNENR 560 +G+K S S A SK+LE++ S R N + + R+ R M + L+ +N Sbjct: 276 LGIKDSNKQISPEHQAILSKRLESV--ESDIR-LEQNTMDMTRIDARKMQMTGDLIMKNS 332 Query: 561 L------GSKGKYQGNGESSARSLSQITSNPAS 641 + G+ G+Y E S + +SQ+ + AS Sbjct: 333 VTVGGIAGASGQYAATQERSEQQISQVNNRVAS 365
>SLA2_YEAST (P33338) Protein SLA2 (Transmembrane protein MOP2)| Length = 968 Score = 30.4 bits (67), Expect = 5.2 Identities = 16/43 (37%), Positives = 21/43 (48%) Frame = -2 Query: 655 LQAVKDAGFEVIWDKDLAEDSPLPWYLPLDPSRFSLSSFRLTT 527 L A+ ++G I + DSPL W PL P F LS T+ Sbjct: 574 LDAILESGINTIQESVYNLDSPLSWSGPLTPPTFLLSLLESTS 616 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 91,611,389 Number of Sequences: 219361 Number of extensions: 1786404 Number of successful extensions: 4338 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 4198 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 4314 length of database: 80,573,946 effective HSP length: 108 effective length of database: 56,882,958 effective search space used: 6769072002 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
