ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd15m03
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1SGAT_ARATH (Q56YA5) Serine--glyoxylate aminotransferase (EC 2.6.... 227 2e-59
2SGAA_METEX (P55819) Serine--glyoxylate aminotransferase (EC 2.6.... 157 3e-38
3SGAA_HYPME (O08374) Serine--glyoxylate aminotransferase (EC 2.6.... 154 2e-37
4Y959_METJA (Q58369) Putative aminotransferase MJ0959 (EC 2.6.1.-) 88 2e-17
5DHSS_ANACY (P16421) Soluble hydrogenase 42 kDa subunit (EC 1.12.... 79 8e-15
6DHSS_SYNP1 (P14776) Soluble hydrogenase, small subunit (EC 1.12.... 74 3e-13
7SPYA_CALJA (P31029) Serine--pyruvate aminotransferase, mitochond... 53 8e-07
8PUCG_BACSU (O32148) Purine catabolism protein pucG (EC 2.-.-.-) 51 2e-06
9SPYA_RAT (P09139) Serine--pyruvate aminotransferase, mitochondri... 49 9e-06
10SPYA_HUMAN (P21549) Serine--pyruvate aminotransferase (EC 2.6.1.... 47 6e-05
11SPYA_FELCA (P41689) Serine--pyruvate aminotransferase, mitochond... 47 6e-05
12SPYA_RABIT (P31030) Serine--pyruvate aminotransferase (EC 2.6.1.... 44 5e-04
13SPYA_MOUSE (O35423) Serine--pyruvate aminotransferase, mitochond... 42 0.001
14SGAT_MAIZE (P84187) Serine--glyoxylate aminotransferase (EC 2.6.... 40 0.006
15CEFD_NOCLA (Q03046) Isopenicillin N epimerase (EC 5.1.1.17) 33 0.89
16LAS17_YEAST (Q12446) Proline-rich protein LAS17 33 0.89
17F100A_RAT (Q6AXN0) Protein FAM100A 32 2.0
18POLN_RUBVT (P13889) Nonstructural polyprotein [Contains: Proteas... 32 2.0
19YGHE_ECOLI (Q46833) Putative general secretion pathway protein L... 30 7.6
20UCN1_HUMAN (P55089) Urocortin precursor 29 9.9

>SGAT_ARATH (Q56YA5) Serine--glyoxylate aminotransferase (EC 2.6.1.45)|
           (Alanine--glyoxylate aminotransferase) (EC 2.6.1.44)
           (AGT) (Serine--pyruvate aminotransferase) (EC 2.6.1.51)
          Length = 401

 Score =  227 bits (579), Expect = 2e-59
 Identities = 109/139 (78%), Positives = 125/139 (89%)
 Frame = -2

Query: 579 LDLLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTVTAVVVPPYIDSSEI 400
           LDL+FEEGL+N+I RH RLG ATRLAV AWGLKNCT+KEE  S+TVTAV+VPP+ID SEI
Sbjct: 263 LDLIFEEGLENIIARHARLGKATRLAVEAWGLKNCTQKEEWISNTVTAVMVPPHIDGSEI 322

Query: 399 VKHAWKRYNLSLGLGLNKVAGKVFRIGHLGHLNELQLLGCLGGVEMVLKDIGYPVKLGSG 220
           V+ AW+RYNLSLGLGLNKVAGKVFRIGHLG++NELQLLGCL GVEM+LKD+GYPV +GSG
Sbjct: 323 VRRAWQRYNLSLGLGLNKVAGKVFRIGHLGNVNELQLLGCLAGVEMILKDVGYPVVMGSG 382

Query: 219 VAAAAAYLSNSTPLIPSRI 163
           VAAA+ YL +  PLIPSRI
Sbjct: 383 VAAASTYLQHHIPLIPSRI 401



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>SGAA_METEX (P55819) Serine--glyoxylate aminotransferase (EC 2.6.1.45) (SGAT)|
          Length = 379

 Score =  157 bits (396), Expect = 3e-38
 Identities = 80/128 (62%), Positives = 97/128 (75%)
 Frame = -2

Query: 579 LDLLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTVTAVVVPPYIDSSEI 400
           L  LFEEGL+NV  RH  LG ATR AVAAWGLK C +  E  SDTVTA++ P  +D+++I
Sbjct: 244 LACLFEEGLENVYHRHAVLGEATRQAVAAWGLKTCAKSPEWNSDTVTAILAPEGVDAAKI 303

Query: 399 VKHAWKRYNLSLGLGLNKVAGKVFRIGHLGHLNELQLLGCLGGVEMVLKDIGYPVKLGSG 220
           +KHA+ RYNL+LG GL++VAGKVFRIGH+G LNEL LLG + G EM L D G  V  GSG
Sbjct: 304 IKHAYVRYNLALGAGLSQVAGKVFRIGHVGDLNELSLLGAIAGAEMSLIDNGVKVTPGSG 363

Query: 219 VAAAAAYL 196
           VAAA++YL
Sbjct: 364 VAAASSYL 371



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>SGAA_HYPME (O08374) Serine--glyoxylate aminotransferase (EC 2.6.1.45) (SGAT)|
          Length = 404

 Score =  154 bits (390), Expect = 2e-37
 Identities = 80/131 (61%), Positives = 95/131 (72%)
 Frame = -2

Query: 579 LDLLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTVTAVVVPPYIDSSEI 400
           LDLLF EGLDNV  RHTRL +  R AV AWGLK C ++ + +SDTV+A++VP  IDS+ I
Sbjct: 258 LDLLFAEGLDNVFARHTRLASGVRAAVDAWGLKLCAKEPKWYSDTVSAILVPEGIDSNAI 317

Query: 399 VKHAWKRYNLSLGLGLNKVAGKVFRIGHLGHLNELQLLGCLGGVEMVLKDIGYPVKLGSG 220
            K A+ RYN S GLGLNKVAGKVFRIGHLG L+E+ + G L   EM LKD G  +KLGSG
Sbjct: 318 TKTAYYRYNTSFGLGLNKVAGKVFRIGHLGMLDEVMIGGALFAAEMALKDNGVNLKLGSG 377

Query: 219 VAAAAAYLSNS 187
             AAA Y S +
Sbjct: 378 TGAAAEYFSKN 388



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>Y959_METJA (Q58369) Putative aminotransferase MJ0959 (EC 2.6.1.-)|
          Length = 385

 Score = 88.2 bits (217), Expect = 2e-17
 Identities = 53/128 (41%), Positives = 75/128 (58%)
 Frame = -2

Query: 579 LDLLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTVTAVVVPPYIDSSEI 400
           LDL+ EEG++N +KRH RL  ATR  + A G++    KE   S TVT+   P  I+ S+ 
Sbjct: 257 LDLVLEEGIENRVKRHERLAKATRAGLEAMGIE-LFAKERARSVTVTSAKYPEGIEDSKF 315

Query: 399 VKHAWKRYNLSLGLGLNKVAGKVFRIGHLGHLNELQLLGCLGGVEMVLKDIGYPVKLGSG 220
                 +YN+ +  G   +AGK+FRIGH+G   E ++L  L  VE+ LK++G+ VK  SG
Sbjct: 316 RGILSNKYNIVVAGGQKHLAGKIFRIGHMGICGEKEVLATLACVELALKELGFEVK-ESG 374

Query: 219 VAAAAAYL 196
           V  A   L
Sbjct: 375 VEVAKEVL 382



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>DHSS_ANACY (P16421) Soluble hydrogenase 42 kDa subunit (EC 1.12.-.-) (Tritium|
           exchange subunit)
          Length = 383

 Score = 79.3 bits (194), Expect = 8e-15
 Identities = 48/142 (33%), Positives = 82/142 (57%), Gaps = 1/142 (0%)
 Frame = -2

Query: 612 PSSSSTAYAPGLDLLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTVTAV 433
           P +   A    L ++ +EGL+++  RH R   ATR A+ A  L      +E  S  +TAV
Sbjct: 244 PVNLMVALHTTLGMMKKEGLESIFTRHERQKNATRAAMKALNLP-LFAADECASPAITAV 302

Query: 432 VVPPYIDSSEIVKHAWKRYNLSLGLGLNKVAGKVFRIGHLGHLNELQLLGCLGGVEMVLK 253
             P  +++ +I     KR++++L  G + ++ K+FR+GHLG +++  +L C+  +E+VL 
Sbjct: 303 ATPG-MEADKIRSLMKKRFDIALAGGQDHLSNKIFRVGHLGFVSDRDILSCIASLEVVLL 361

Query: 252 DIGYP-VKLGSGVAAAAAYLSN 190
           ++G+     G+GVAAAA   SN
Sbjct: 362 ELGHENFNSGAGVAAAARVFSN 383



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>DHSS_SYNP1 (P14776) Soluble hydrogenase, small subunit (EC 1.12.-.-) (Tritium|
           exchange subunit)
          Length = 384

 Score = 73.9 bits (180), Expect = 3e-13
 Identities = 47/137 (34%), Positives = 77/137 (56%), Gaps = 1/137 (0%)
 Frame = -2

Query: 594 AYAPGLDLLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTVTAVVVPPYI 415
           A    L+++  EGL+ + +RH RL  ATR A+ A  L      +   S  +TAV  P  +
Sbjct: 250 ALKTALEMMQAEGLEAIFQRHQRLMQATRAAMKALNLP-LYAADSCASPAITAVA-PQGV 307

Query: 414 DSSEIVKHAWKRYNLSLGLGLNKVAGKVFRIGHLGHLNELQLLGCLGGVEMVLKDIGYP- 238
           ++  I     KR++++L  G + + G++FRIGHLG + +  +L  +  +E VL ++GY  
Sbjct: 308 EAENIRSLMKKRFDIALAGGQDHLKGQIFRIGHLGFVGDRDILAAVSALEAVLAELGYTN 367

Query: 237 VKLGSGVAAAAAYLSNS 187
              G+GVAAA+  LS +
Sbjct: 368 FTPGAGVAAASRVLSTA 384



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>SPYA_CALJA (P31029) Serine--pyruvate aminotransferase, mitochondrial precursor|
           (EC 2.6.1.51) (SPT) (Alanine--glyoxylate
           aminotransferase) (EC 2.6.1.44) (AGT)
          Length = 414

 Score = 52.8 bits (125), Expect = 8e-07
 Identities = 34/104 (32%), Positives = 49/104 (47%)
 Frame = -2

Query: 621 HARPSSSSTAYAPGLDLLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTV 442
           H  P  S  +   GL LL E+GL+N  ++H          + A GL+   +       TV
Sbjct: 284 HTTPVVSLYSLREGLALLSEQGLENSWRKHREAAAYLHGRLQALGLRLFVKDPALRLPTV 343

Query: 441 TAVVVPPYIDSSEIVKHAWKRYNLSLGLGLNKVAGKVFRIGHLG 310
           T V VP   D  +IV +  +R+ + +  GL    GKV RIG +G
Sbjct: 344 TTVAVPTGYDWRDIVSYLIERFGIEITGGLGPSTGKVLRIGLMG 387



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>PUCG_BACSU (O32148) Purine catabolism protein pucG (EC 2.-.-.-)|
          Length = 416

 Score = 51.2 bits (121), Expect = 2e-06
 Identities = 37/146 (25%), Positives = 64/146 (43%), Gaps = 1/146 (0%)
 Frame = -2

Query: 621 HARPSSSSTAYAPGLDLLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTV 442
           H   ++   A   G+ L+ EEGL+   +RH     A    + A GL+   +        V
Sbjct: 266 HTEATTMLYALREGVRLVLEEGLETRFERHRHHEAALAAGIKAMGLRLFGDDSCKMP-VV 324

Query: 441 TAVVVPPYIDSSEIVKHAWKRYNLSLGLGLNKVAGKVFRIGHLGH-LNELQLLGCLGGVE 265
           T V +P  ID   +      ++ + +      +AGK++RIG +G+   +  +L  L G+E
Sbjct: 325 TCVEIPGGIDGESVRDMLLAQFGIEIASSFGPLAGKIWRIGTMGYSCRKENVLFVLAGLE 384

Query: 264 MVLKDIGYPVKLGSGVAAAAAYLSNS 187
            VL      ++ G  + AA     N+
Sbjct: 385 AVLLRHNAGIEAGKALQAALDVYENA 410



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>SPYA_RAT (P09139) Serine--pyruvate aminotransferase, mitochondrial precursor|
           (EC 2.6.1.51) (SPT) (Alanine--glyoxylate
           aminotransferase) (EC 2.6.1.44) (AGT)
          Length = 414

 Score = 49.3 bits (116), Expect = 9e-06
 Identities = 32/105 (30%), Positives = 46/105 (43%)
 Frame = -2

Query: 621 HARPSSSSTAYAPGLDLLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTV 442
           H  P  S       L L+ E+GL+N  +RH          +   GLK   +  E    T+
Sbjct: 284 HTLPVISLYCLRESLALISEQGLENSWRRHREATAHLHKCLRELGLKFFVKDPEIRLPTI 343

Query: 441 TAVVVPPYIDSSEIVKHAWKRYNLSLGLGLNKVAGKVFRIGHLGH 307
           T V VP   +  +IV +    +N+ +  GL     KV RIG LG+
Sbjct: 344 TTVTVPAGYNWRDIVSYVLDHFNIEISGGLGPSEDKVLRIGLLGY 388



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>SPYA_HUMAN (P21549) Serine--pyruvate aminotransferase (EC 2.6.1.51) (SPT)|
           (Alanine--glyoxylate aminotransferase) (EC 2.6.1.44)
           (AGT)
          Length = 392

 Score = 46.6 bits (109), Expect = 6e-05
 Identities = 32/104 (30%), Positives = 47/104 (45%)
 Frame = -2

Query: 621 HARPSSSSTAYAPGLDLLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTV 442
           H  P  S  +    L L+ E+GL+N  ++H          + A GL+   +       TV
Sbjct: 262 HTIPVISLYSLRESLALIAEQGLENSWRQHREAAAYLHGRLQALGLQLFVKDPALRLPTV 321

Query: 441 TAVVVPPYIDSSEIVKHAWKRYNLSLGLGLNKVAGKVFRIGHLG 310
           T V VP   D  +IV +    +++ +  GL    GKV RIG LG
Sbjct: 322 TTVAVPAGYDWRDIVSYVIDHFDIEIMGGLGPSTGKVLRIGLLG 365



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>SPYA_FELCA (P41689) Serine--pyruvate aminotransferase, mitochondrial precursor|
           (EC 2.6.1.51) (SPT) (Alanine--glyoxylate
           aminotransferase) (EC 2.6.1.44) (AGT)
          Length = 414

 Score = 46.6 bits (109), Expect = 6e-05
 Identities = 31/104 (29%), Positives = 47/104 (45%)
 Frame = -2

Query: 621 HARPSSSSTAYAPGLDLLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTV 442
           H  P  S  +    L L+ E+GL+N  ++H  +       +   GL+   +       TV
Sbjct: 284 HTTPVVSLYSLRESLALIAEQGLENSWRQHREVTAYLHGRLQGLGLQLFVKDPALRLPTV 343

Query: 441 TAVVVPPYIDSSEIVKHAWKRYNLSLGLGLNKVAGKVFRIGHLG 310
           T V VP   D  +IV +    +++ +  GL    GKV RIG LG
Sbjct: 344 TTVAVPAGYDWRDIVNYVMDHFDIEITGGLGPSMGKVLRIGLLG 387



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>SPYA_RABIT (P31030) Serine--pyruvate aminotransferase (EC 2.6.1.51) (SPT)|
           (Alanine--glyoxylate aminotransferase) (EC 2.6.1.44)
           (AGT)
          Length = 392

 Score = 43.5 bits (101), Expect = 5e-04
 Identities = 31/104 (29%), Positives = 44/104 (42%)
 Frame = -2

Query: 621 HARPSSSSTAYAPGLDLLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTV 442
           H  P     A    L LL E+GL+   +RH  +       +   GL+   +       TV
Sbjct: 262 HTTPVIGIFALRESLALLVEQGLEKSWQRHREVAQHLYRRLQELGLQLFVKDPALRLPTV 321

Query: 441 TAVVVPPYIDSSEIVKHAWKRYNLSLGLGLNKVAGKVFRIGHLG 310
           T V+VP      +IV +    + + +  GL   A KV RIG LG
Sbjct: 322 TTVIVPASYRWRDIVSYVMHHFGIEITGGLGPSADKVLRIGLLG 365



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>SPYA_MOUSE (O35423) Serine--pyruvate aminotransferase, mitochondrial precursor|
           (EC 2.6.1.51) (SPT) (Alanine--glyoxylate
           aminotransferase) (EC 2.6.1.44) (AGT)
          Length = 413

 Score = 42.0 bits (97), Expect = 0.001
 Identities = 29/105 (27%), Positives = 45/105 (42%)
 Frame = -2

Query: 621 HARPSSSSTAYAPGLDLLFEEGLDNVIKRHTRLGTATRLAVAAWGLKNCTEKEENFSDTV 442
           H  P +S       L L+ E GL+N  +RH          +   GLK   +  E    T+
Sbjct: 284 HTTPVTSLYCLRESLALIAERGLENCWRRHREATAHLHKHLQEMGLKFFVKDPEIRLPTI 343

Query: 441 TAVVVPPYIDSSEIVKHAWKRYNLSLGLGLNKVAGKVFRIGHLGH 307
           T V    Y +  +IV +    +++ +  GL     +V RIG LG+
Sbjct: 344 TTVTAAGY-NWRDIVSYVLDHFSIEISGGLGPTEERVLRIGLLGY 387



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>SGAT_MAIZE (P84187) Serine--glyoxylate aminotransferase (EC 2.6.1.45) (SGAT)|
           (Alanine--glyoxylate aminotransferase) (EC 2.6.1.44)
           (AGT) (Fragments)
          Length = 136

 Score = 40.0 bits (92), Expect = 0.006
 Identities = 28/62 (45%), Positives = 30/62 (48%), Gaps = 4/62 (6%)
 Frame = -2

Query: 405 EIVKHAW---KRYNLSLGLGLNKVA-GKVFRIGHLGHLNELQLLGCLGGVEMVLKDIGYP 238
           E+   AW    RYNLSLGLGLNKVA GKVFR                        D+GYP
Sbjct: 99  ELAVEAWGLSNRYNLSLGLGLNKVAGGKVFR------------------------DVGYP 134

Query: 237 VK 232
           VK
Sbjct: 135 VK 136



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>CEFD_NOCLA (Q03046) Isopenicillin N epimerase (EC 5.1.1.17)|
          Length = 398

 Score = 32.7 bits (73), Expect = 0.89
 Identities = 23/85 (27%), Positives = 42/85 (49%), Gaps = 3/85 (3%)
 Frame = -2

Query: 567 FEEGLDN--VIKRHTRLGTATRLAVAAW-GLKNCTEKEENFSDTVTAVVVPPYIDSSEIV 397
           F+ GL +  V +R   L   TR  ++ W GL+  T      S  +TA  +PP  D++ + 
Sbjct: 292 FQAGLGHGRVRERMRELAAFTRERLSGWRGLEPVTPAHPGLSGAMTAFRLPPGTDTAGLR 351

Query: 396 KHAWKRYNLSLGLGLNKVAGKVFRI 322
              W R+ + + + L++  G + R+
Sbjct: 352 HGLWDRFRIDVPV-LDRPDGPLLRV 375



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>LAS17_YEAST (Q12446) Proline-rich protein LAS17|
          Length = 633

 Score = 32.7 bits (73), Expect = 0.89
 Identities = 16/32 (50%), Positives = 17/32 (53%), Gaps = 1/32 (3%)
 Frame = +3

Query: 189 NSRGTPPRPPRRCQAS-PGTRCP*APSPPRRG 281
           N+R  PP PP R      G R P  P PPRRG
Sbjct: 320 NNRPVPPPPPMRTTTEGSGVRLPAPPPPPRRG 351



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>F100A_RAT (Q6AXN0) Protein FAM100A|
          Length = 176

 Score = 31.6 bits (70), Expect = 2.0
 Identities = 15/44 (34%), Positives = 20/44 (45%)
 Frame = +2

Query: 191 FERYAAAAATPLPSFTGYPMSLSTISTPPRQPRSCSSLRWPRWP 322
           F R  A+ +      +G PM+ S  S PP  P +  S   P WP
Sbjct: 81  FSRLKASESFHSGGSSGSPMATSATSPPPHFPHATGSFATPSWP 124



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>POLN_RUBVT (P13889) Nonstructural polyprotein [Contains: Protease (EC|
           3.4.22.-) (p150); RNA-directed RNA polymerase/helicase
           (EC 2.7.7.48) (EC 3.6.1.-) (p90)]
          Length = 2115

 Score = 31.6 bits (70), Expect = 2.0
 Identities = 15/33 (45%), Positives = 19/33 (57%)
 Frame = +3

Query: 189 NSRGTPPRPPRRCQASPGTRCP*APSPPRRGNP 287
           ++RGTPP  P R    P    P  P+PPR G+P
Sbjct: 740 DARGTPPPAPAR---DPPPPAPSPPAPPRAGDP 769



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>YGHE_ECOLI (Q46833) Putative general secretion pathway protein L-type yghE|
          Length = 286

 Score = 29.6 bits (65), Expect = 7.6
 Identities = 17/53 (32%), Positives = 22/53 (41%)
 Frame = +2

Query: 167 LEGMRGVEFERYAAAAATPLPSFTGYPMSLSTISTPPRQPRSCSSLRWPRWPI 325
           L    G + ER    A   LP+ T Y      + T P QPR     +W RW +
Sbjct: 87  LSAWAGADAERLTVTA---LPAITTYGEPEGNLLTGPWQPRVSYRKQWARWRV 136



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>UCN1_HUMAN (P55089) Urocortin precursor|
          Length = 124

 Score = 29.3 bits (64), Expect = 9.9
 Identities = 18/56 (32%), Positives = 27/56 (48%)
 Frame = -1

Query: 280 PRRGGDGAQGHRVPGEAWQRRGGRGGVPLEFHAPHPLQNLSKFHAQKQGGREEKNK 113
           PRR G G  G    GE  +R      + L FH    L  L++  +Q++  R E+N+
Sbjct: 63  PRRAGPGRLGLGTAGERPRRDNPSLSIDLTFHLLRTLLELARTQSQRE--RAEQNR 116


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,512,128
Number of Sequences: 219361
Number of extensions: 1465873
Number of successful extensions: 6127
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 5445
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6096
length of database: 80,573,946
effective HSP length: 107
effective length of database: 57,102,319
effective search space used: 5710231900
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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