ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | rbasd15j23 |
|---|---|
| Clone Library Name | barley_pub |
>SAP_HUMAN (P07602) Proactivator polypeptide precursor [Contains: Saposin A| (Protein A); Saposin B-Val; Saposin B (Sphingolipid activator protein 1) (SAP-1) (Cerebroside sulfate activator) (CSAct) (Dispersin) (Sulfatide/GM1 activator); Saposin C (Co-beta- Length = 524 Score = 55.5 bits (132), Expect = 1e-07 Identities = 35/141 (24%), Positives = 71/141 (50%), Gaps = 8/141 (5%) Frame = -1 Query: 617 SLEKKCVELVDSY-ATLLFAKIAEIKPEEFCKRNGLCRDNAL------LSGVRSESTCVF 459 SL ++C E+VD+Y +++L + E+ PE C LC L ++ + C Sbjct: 352 SLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGGFCEV 411 Query: 458 CHHLMDEVLSKLKDPDAEFEIIQILLKECKKI-EGHEQQCKRLVLQYIPLILVNGEKFLE 282 C L+ + L+ + EI+ L K C + + +++QC + V +Y P+++ + ++ Sbjct: 412 CKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMD 471 Query: 281 KNDICTIVQACDAGKKTVLGS 219 + +C + AC + K +LG+ Sbjct: 472 PSFVCLKIGACPSAHKPLLGT 492 Score = 36.2 bits (82), Expect = 0.086 Identities = 28/109 (25%), Positives = 49/109 (44%), Gaps = 4/109 (3%) Frame = -1 Query: 467 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKI--EGHEQQCKRLVLQYIPLIL--VN 300 C C ++ LKD E EI+ L K C + CK +V Y+P+IL + Sbjct: 63 CDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIK 122 Query: 299 GEKFLEKNDICTIVQACDAGKKTVLGSFSEEGLLRDE*RQKDYTEPRKP 153 GE ++C+ + C++ +K + ++ L ++ + D TE P Sbjct: 123 GE-MSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIPELDMTEVVAP 170
>SAP_BOVIN (P26779) Proactivator polypeptide precursor [Contains: Saposin A| (Protein A); Saposin B (Sphingolipid activator protein 1) (SAP-1) (Cerebroside sulfate activator) (CSAct) (Dispersin) (Sulfatide/GM1 activator); Saposin C (Co-beta-glucosidase) (A Length = 525 Score = 53.9 bits (128), Expect = 4e-07 Identities = 38/151 (25%), Positives = 72/151 (47%), Gaps = 9/151 (5%) Frame = -1 Query: 644 LHEACSQT-FSLEKKCVELVDSYA-TLLFAKIAEIKPEEFCKRNGLCRDNALLSGV---- 483 L + CS+ SL ++C E+VD+Y ++L + E PE C LC L + Sbjct: 343 LDKVCSKLPTSLAEQCQEVVDTYGRSILSILLDEASPELVCSMLHLCSSRGLPAATVRVM 402 Query: 482 --RSESTCVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKI-EGHEQQCKRLVLQYIPL 312 + C C L+ + L+ + +I+ L K C + + + +QC + V +Y P+ Sbjct: 403 PRKDGGFCEVCKKLVGYLDRNLEKNSTKEQILAALEKGCSFLPDQYRKQCDQFVTEYEPV 462 Query: 311 ILVNGEKFLEKNDICTIVQACDAGKKTVLGS 219 ++ + ++ + +C + AC A K +LG+ Sbjct: 463 LIEILVEVMDPSFVCLKIGACPAAHKPLLGA 493 Score = 33.5 bits (75), Expect = 0.56 Identities = 21/82 (25%), Positives = 39/82 (47%), Gaps = 4/82 (4%) Frame = -1 Query: 467 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKIE--GHEQQCKRLVLQYIPLIL--VN 300 C C ++ + LKD E EI+ L + C + CK +V Y+P+IL + Sbjct: 63 CDICKDVITAAGNLLKDNATEQEILMYLERTCDWLPKPNMSASCKEIVDSYLPVILDMIK 122 Query: 299 GEKFLEKNDICTIVQACDAGKK 234 G+ ++C+ + C++ +K Sbjct: 123 GQ-MSHPGEVCSALNLCESLQK 143
>SAP_CHICK (O13035) Proactivator polypeptide precursor [Contains: Saposin A;| Saposin B; Saposin C; Saposin D] Length = 518 Score = 45.4 bits (106), Expect = 1e-04 Identities = 31/123 (25%), Positives = 58/123 (47%), Gaps = 3/123 (2%) Frame = -1 Query: 512 CRDNALLSGVRSESTCVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKI--EGHEQQCK 339 C+ N + C C L+ V LKD E EI L K C+ + +G +CK Sbjct: 49 CQQNVWSKPAVNSIPCDLCKELVTVVGKVLKDNGTEDEIRSYLEKRCEFLPDQGLASECK 108 Query: 338 RLVLQYIPLIL-VNGEKFLEKNDICTIVQACDAGKKTVLGSFSEEGLLRDE*RQKDYTEP 162 +V Y+P+I+ + E+F + +C+ + C + +K + ++ L ++ + D++E Sbjct: 109 EIVDSYLPVIMDMIKEEFDKPEVVCSALSLCQSLQKHLAAMKLQKQLQSNKIPELDFSEL 168 Query: 161 RKP 153 P Sbjct: 169 TSP 171 Score = 40.4 bits (93), Expect = 0.005 Identities = 33/138 (23%), Positives = 58/138 (42%), Gaps = 6/138 (4%) Frame = -1 Query: 617 SLEKKCVELVDSYATLLFAKIAEI-KPEEFCKRNGLCRDNAL----LSGVRSESTCVFCH 453 S++ +C + ++ Y L + E PE C C N + + C C Sbjct: 348 SVKDQCKDFIEVYGQALIDMLLEATNPEAVCVMLKCCAANKPPQQPVVVKPAGGFCDICK 407 Query: 452 HLMDEVLSKLKDPDAEFEIIQILLKECKKI-EGHEQQCKRLVLQYIPLILVNGEKFLEKN 276 ++ +L+ EI +L K C + E QC + V QY P+++ + ++ Sbjct: 408 MIVAYADKELEKNATTTEIEALLEKVCHFLPESVSDQCVQFVEQYEPVVVQLLAEMMDPT 467 Query: 275 DICTIVQACDAGKKTVLG 222 +CT + C A KK +LG Sbjct: 468 FVCTKLGVCGAAKKPLLG 485
>SAP_MOUSE (Q61207) Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)| Length = 557 Score = 40.0 bits (92), Expect = 0.006 Identities = 38/168 (22%), Positives = 67/168 (39%), Gaps = 39/168 (23%) Frame = -1 Query: 605 KCVELVDSYA-TLLFAKIAEIKPEEFCKRNGLCRDN------------ALLSGVRSEST- 468 KC E+V ++ +LL I E+ P C GLC A++S + E T Sbjct: 358 KCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTP 417 Query: 467 ------------------------CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKI- 363 C C L+ + L+ + EI+ L K C + Sbjct: 418 PKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLP 477 Query: 362 EGHEQQCKRLVLQYIPLILVNGEKFLEKNDICTIVQACDAGKKTVLGS 219 + +++QC V +Y PL+L + ++ +C+ + C + K +LG+ Sbjct: 478 DPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGT 525 Score = 36.6 bits (83), Expect = 0.066 Identities = 23/79 (29%), Positives = 38/79 (48%), Gaps = 4/79 (5%) Frame = -1 Query: 467 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKIEGH--EQQCKRLVLQYIPLIL--VN 300 C C ++ E + LKD + EI+ L K C+ I CK +V Y+P+IL + Sbjct: 63 CDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIK 122 Query: 299 GEKFLEKNDICTIVQACDA 243 GE ++C+ + C + Sbjct: 123 GE-MSNPGEVCSALNLCQS 140
>SAP_RAT (P10960) Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)| Length = 554 Score = 38.1 bits (87), Expect = 0.023 Identities = 21/84 (25%), Positives = 43/84 (51%), Gaps = 1/84 (1%) Frame = -1 Query: 467 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKI-EGHEQQCKRLVLQYIPLILVNGEK 291 C C L+ + L+ + EI+ L K C + + +++QC V +Y PL+L + Sbjct: 439 CEVCKKLVIYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEPLLLEILVE 498 Query: 290 FLEKNDICTIVQACDAGKKTVLGS 219 ++ + +C+ + C + K +LG+ Sbjct: 499 VMDPSFVCSKIGVCPSAYKLLLGT 522 Score = 38.1 bits (87), Expect = 0.023 Identities = 27/107 (25%), Positives = 44/107 (41%), Gaps = 4/107 (3%) Frame = -1 Query: 551 EIKPEEFCKRNGLCRDNALLSGVRSESTCVFCHHLMDEVLSKLKDPDAEFEIIQILLKEC 372 ++K C+ C+ C C ++ E + LKD E EI+ L K C Sbjct: 35 DVKTAVDCRAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATEEEILHYLEKTC 94 Query: 371 KKIEGH--EQQCKRLVLQYIPLIL--VNGEKFLEKNDICTIVQACDA 243 I CK +V Y+P+IL + GE ++C+ + C + Sbjct: 95 AWIHDSSLSASCKEVVDSYLPVILDMIKGE-MSNPGEVCSALNLCQS 140
>ATG11_CANAL (Q5AMN3) Autophagy-related protein 11| Length = 1165 Score = 34.3 bits (77), Expect = 0.33 Identities = 25/77 (32%), Positives = 39/77 (50%) Frame = -1 Query: 632 CSQTFSLEKKCVELVDSYATLLFAKIAEIKPEEFCKRNGLCRDNALLSGVRSESTCVFCH 453 C + +L KK +EL D++ L AKI E + K + N +S +RSE Sbjct: 742 CDEVENLTKK-LELSDNHNKELEAKITEYTQKATSKTKEIADLNKTVSNLRSELGDAM-- 798 Query: 452 HLMDEVLSKLKDPDAEF 402 H+ +++LS L +AEF Sbjct: 799 HMKNDLLSNLSSKEAEF 815
>OXAA_DEIRA (Q9RSH5) Membrane protein oxaA| Length = 428 Score = 33.9 bits (76), Expect = 0.43 Identities = 16/40 (40%), Positives = 24/40 (60%) Frame = +3 Query: 93 ASRILDTYVQQEELVHYVSSGFTRLGVVFLPSFIAQQTLL 212 A+ L+ Y + EL+H SG+T L +F P+F Q +LL Sbjct: 159 ATSSLEVYGGKNELIHLYQSGYTELPGLFQPNFFGQISLL 198
>NF1_MOUSE (Q04690) Neurofibromin (Neurofibromatosis-related protein NF-1)| Length = 2841 Score = 32.3 bits (72), Expect = 1.2 Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 5/64 (7%) Frame = -1 Query: 452 HLMDEVLSKLKDPDAEFEII-----QILLKECKKIEGHEQQCKRLVLQYIPLILVNGEKF 288 H +D + L +PDA E Q+L CKK+ H+ +L+++ IL+ KF Sbjct: 553 HQLDSI--DLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKF 610 Query: 287 LEKN 276 L KN Sbjct: 611 LLKN 614
>NF1_HUMAN (P21359) Neurofibromin (Neurofibromatosis-related protein NF-1)| [Contains: Neurofibromin truncated] Length = 2839 Score = 32.3 bits (72), Expect = 1.2 Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 5/64 (7%) Frame = -1 Query: 452 HLMDEVLSKLKDPDAEFEII-----QILLKECKKIEGHEQQCKRLVLQYIPLILVNGEKF 288 H +D + L +PDA E Q+L CKK+ H+ +L+++ IL+ KF Sbjct: 553 HQLDSI--DLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKF 610 Query: 287 LEKN 276 L KN Sbjct: 611 LLKN 614
>NF1_RAT (P97526) Neurofibromin (Neurofibromatosis-related protein NF-1)| Length = 2820 Score = 32.3 bits (72), Expect = 1.2 Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 5/64 (7%) Frame = -1 Query: 452 HLMDEVLSKLKDPDAEFEII-----QILLKECKKIEGHEQQCKRLVLQYIPLILVNGEKF 288 H +D + L +PDA E Q+L CKK+ H+ +L+++ IL+ KF Sbjct: 553 HQLDSI--DLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKF 610 Query: 287 LEKN 276 L KN Sbjct: 611 LLKN 614
>RY44_DROME (Q24498) Ryanodine receptor 44F| Length = 5127 Score = 30.4 bits (67), Expect = 4.7 Identities = 16/59 (27%), Positives = 26/59 (44%), Gaps = 6/59 (10%) Frame = -2 Query: 646 SFMKLVLRHSRWRRSVLSWWTPMQLSYLPRSRRSSQKSS------ANEMASAGTMLFCL 488 S +L L ++W ++W+ PM++ ++ R S E AS T FCL Sbjct: 257 SLWRLELARTKWTGGFINWYHPMRIRHITTGRYLGVNDSNELILVKKEEASIATTTFCL 315
>GLRX1_YEAST (P25373) Glutaredoxin-1 (Glutathione-dependent oxidoreductase 1)| Length = 110 Score = 29.6 bits (65), Expect = 8.0 Identities = 21/75 (28%), Positives = 40/75 (53%), Gaps = 3/75 (4%) Frame = -1 Query: 485 VRSESTCVFCHHLMDEVLSKLKDPDAEFEIIQIL-LKECKKIEG--HEQQCKRLVLQYIP 315 V S++ C +CH ++ + KLK P ++ ++Q+ +KE I+ +E +R V P Sbjct: 21 VASKTYCPYCHAALNTLFEKLKVPRSKVLVLQLNDMKEGADIQAALYEINGQRTV----P 76 Query: 314 LILVNGEKFLEKNDI 270 I +NG+ +D+ Sbjct: 77 NIYINGKHIGGNDDL 91
>SEN54_SCHPO (O74908) Probable tRNA-splicing endonuclease subunit sen54| (tRNA-intron endonuclease sen54) Length = 384 Score = 29.6 bits (65), Expect = 8.0 Identities = 11/32 (34%), Positives = 19/32 (59%) Frame = -3 Query: 291 VPREERYMHHRSSMRCWQKDGARVILRGGFAA 196 +P E Y+ R SM CW ++G + L+ ++A Sbjct: 120 LPEETLYLVERGSMECWSEEGLPMSLQAVYSA 151
>SYP32_ARATH (Q9LK09) Syntaxin-32 (AtSYP32)| Length = 347 Score = 29.6 bits (65), Expect = 8.0 Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 3/55 (5%) Frame = +3 Query: 48 IIQSIQRMQQQHAAKASRILDTYVQ-QEELVHYVSSGFTRLGVVF--LPSFIAQQ 203 ++Q Q+ QQQ + + DTY+Q + E +H V S L +F L + ++QQ Sbjct: 235 LLQQSQQQQQQQQQQMVPLQDTYMQGRAEALHTVESTIHELSSIFTQLATMVSQQ 289 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 93,934,674 Number of Sequences: 219361 Number of extensions: 1947576 Number of successful extensions: 5765 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 5553 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 5760 length of database: 80,573,946 effective HSP length: 108 effective length of database: 56,882,958 effective search space used: 6086476506 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
