ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd14p02
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1MIOX_ORYSA (Q5Z8T3) Probable inositol oxygenase (EC 1.13.99.1) (... 273 3e-73
2MIOX5_ARATH (Q9FJU4) Inositol oxygenase 5 (EC 1.13.99.1) (Myo-in... 255 9e-68
3MIOX4_ARATH (Q8H1S0) Inositol oxygenase 4 (EC 1.13.99.1) (Myo-in... 249 5e-66
4MIOX1_ARATH (Q8L799) Inositol oxygenase 1 (EC 1.13.99.1) (Myo-in... 242 6e-64
5MIOX2_ARATH (O82200) Inositol oxygenase 2 (EC 1.13.99.1) (Myo-in... 239 4e-63
6MIOX_BRARE (Q4V8T0) Inositol oxygenase (EC 1.13.99.1) (Myo-inosi... 164 2e-40
7MIOX_PIG (Q8WN98) Inositol oxygenase (EC 1.13.99.1) (Myo-inosito... 145 1e-34
8MIOX_MOUSE (Q9QXN5) Inositol oxygenase (EC 1.13.99.1) (Myo-inosi... 144 2e-34
9MIOX_PONPY (Q5REY9) Inositol oxygenase (EC 1.13.99.1) (Myo-inosi... 140 2e-33
10MIOX_RAT (Q9QXN4) Inositol oxygenase (EC 1.13.99.1) (Myo-inosito... 140 3e-33
11MIOX_HUMAN (Q9UGB7) Inositol oxygenase (EC 1.13.99.1) (Myo-inosi... 140 4e-33
12CSK_CHICK (P41239) Tyrosine-protein kinase CSK (EC 2.7.10.2) (C-... 32 1.2
13SPB1_CRYNE (Q5KM86) AdoMet-dependent rRNA methyltransferase SPB1... 32 1.2
14TOP1_RICPR (Q9ZDK2) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoi... 30 4.6
15GRPE_THEMA (Q9WZV4) Protein grpE (HSP-70 cofactor) 30 6.1
16RIN2_HUMAN (Q8WYP3) Ras and Rab interactor 2 (Ras interaction/in... 30 6.1

>MIOX_ORYSA (Q5Z8T3) Probable inositol oxygenase (EC 1.13.99.1) (Myo-inositol|
           oxygenase)
          Length = 308

 Score =  273 bits (698), Expect = 3e-73
 Identities = 124/136 (91%), Positives = 128/136 (94%)
 Frame = -2

Query: 637 AFDECNVHFKYLKENPDYHNPEFNTKFGVYSEGCGLDNVLMSWGHDDYMYLVAKENKTTL 458
           AFDECNVHFKY KENPDY NP+ NTKFG YSEGCGLDNVLMSWGHDDYMYLVAKENKTTL
Sbjct: 173 AFDECNVHFKYFKENPDYLNPKLNTKFGAYSEGCGLDNVLMSWGHDDYMYLVAKENKTTL 232

Query: 457 PSAGLFIIRYHSFYPLHKHGAYTHLMNEEDKENLKWLHVFNKYDLYSKSSVRIDVEKVKP 278
           PSAGLFIIRYHSFYPLHKHGAY HLMN+EDKENLKWL VFNKYDLYSKS+ RIDVEKVKP
Sbjct: 233 PSAGLFIIRYHSFYPLHKHGAYMHLMNDEDKENLKWLRVFNKYDLYSKSNERIDVEKVKP 292

Query: 277 YYMSLIEKYFPEKLKW 230
           YYMSLIEKYFP KL+W
Sbjct: 293 YYMSLIEKYFPAKLRW 308



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>MIOX5_ARATH (Q9FJU4) Inositol oxygenase 5 (EC 1.13.99.1) (Myo-inositol|
           oxygenase 5) (AtMIOX5)
          Length = 314

 Score =  255 bits (651), Expect = 9e-68
 Identities = 114/136 (83%), Positives = 125/136 (91%)
 Frame = -2

Query: 637 AFDECNVHFKYLKENPDYHNPEFNTKFGVYSEGCGLDNVLMSWGHDDYMYLVAKENKTTL 458
           AFDE NVH KY  ENPD++NP++NTK G+YSEGCGL+NVLMSWGHDDYMYLVAKEN +TL
Sbjct: 179 AFDESNVHHKYFMENPDFNNPKYNTKAGIYSEGCGLENVLMSWGHDDYMYLVAKENGSTL 238

Query: 457 PSAGLFIIRYHSFYPLHKHGAYTHLMNEEDKENLKWLHVFNKYDLYSKSSVRIDVEKVKP 278
           PS GLFIIRYHSFYPLHK GAYTHLMNEEDKENLKWLHVFNKYDLYSKS V ++VEKVKP
Sbjct: 239 PSPGLFIIRYHSFYPLHKAGAYTHLMNEEDKENLKWLHVFNKYDLYSKSKVHVNVEKVKP 298

Query: 277 YYMSLIEKYFPEKLKW 230
           YYMSLI+KYFPE L+W
Sbjct: 299 YYMSLIKKYFPENLRW 314



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>MIOX4_ARATH (Q8H1S0) Inositol oxygenase 4 (EC 1.13.99.1) (Myo-inositol|
           oxygenase 4) (AtMIOX4)
          Length = 317

 Score =  249 bits (636), Expect = 5e-66
 Identities = 112/136 (82%), Positives = 121/136 (88%)
 Frame = -2

Query: 637 AFDECNVHFKYLKENPDYHNPEFNTKFGVYSEGCGLDNVLMSWGHDDYMYLVAKENKTTL 458
           AFDE NVH KY  ENPD+HN  +NTK G+YSEGCGL+NV+MSWGHDDYMYLVAKEN +TL
Sbjct: 182 AFDESNVHHKYFVENPDFHNETYNTKNGIYSEGCGLNNVMMSWGHDDYMYLVAKENGSTL 241

Query: 457 PSAGLFIIRYHSFYPLHKHGAYTHLMNEEDKENLKWLHVFNKYDLYSKSSVRIDVEKVKP 278
           PSAG FIIRYHSFYPLH  G YTHLMNEEDKENLKWLHVFNKYDLYSKS V +DVEKVKP
Sbjct: 242 PSAGQFIIRYHSFYPLHTAGEYTHLMNEEDKENLKWLHVFNKYDLYSKSKVHVDVEKVKP 301

Query: 277 YYMSLIEKYFPEKLKW 230
           YYMSLI+KYFPE L+W
Sbjct: 302 YYMSLIKKYFPENLRW 317



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>MIOX1_ARATH (Q8L799) Inositol oxygenase 1 (EC 1.13.99.1) (Myo-inositol|
           oxygenase 1) (AtMIOX1)
          Length = 311

 Score =  242 bits (618), Expect = 6e-64
 Identities = 108/136 (79%), Positives = 120/136 (88%)
 Frame = -2

Query: 637 AFDECNVHFKYLKENPDYHNPEFNTKFGVYSEGCGLDNVLMSWGHDDYMYLVAKENKTTL 458
           AFDE  VH KY KENPDY NP +N+K+G+Y+EGCGLDNVLMSWGHDDYMYLVAKEN+TTL
Sbjct: 176 AFDESIVHHKYFKENPDYDNPSYNSKYGIYTEGCGLDNVLMSWGHDDYMYLVAKENQTTL 235

Query: 457 PSAGLFIIRYHSFYPLHKHGAYTHLMNEEDKENLKWLHVFNKYDLYSKSSVRIDVEKVKP 278
           PSAGLFIIRYHSFY LHK  AY HLMN ED+EN+KWL VFNKYDLYSKS VR++VE+VKP
Sbjct: 236 PSAGLFIIRYHSFYALHKSEAYKHLMNNEDRENMKWLKVFNKYDLYSKSKVRVNVEEVKP 295

Query: 277 YYMSLIEKYFPEKLKW 230
           YY+SL  KYFP KLKW
Sbjct: 296 YYLSLTNKYFPSKLKW 311



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>MIOX2_ARATH (O82200) Inositol oxygenase 2 (EC 1.13.99.1) (Myo-inositol|
           oxygenase 2) (AtMIOX2)
          Length = 317

 Score =  239 bits (611), Expect = 4e-63
 Identities = 107/135 (79%), Positives = 120/135 (88%)
 Frame = -2

Query: 634 FDECNVHFKYLKENPDYHNPEFNTKFGVYSEGCGLDNVLMSWGHDDYMYLVAKENKTTLP 455
           FD  N+H KY K N D +NP++NTK GVY+EGCGLDNVLMSWGHDDYMYLVAK+N TTLP
Sbjct: 183 FDSANIHHKYFKGNHDINNPKYNTKNGVYTEGCGLDNVLMSWGHDDYMYLVAKKNGTTLP 242

Query: 454 SAGLFIIRYHSFYPLHKHGAYTHLMNEEDKENLKWLHVFNKYDLYSKSSVRIDVEKVKPY 275
            AGLFIIRYHSFYPLHK GAYTHLMN+ED+++LKWLHVFNKYDLYSKS V +DVE+VKPY
Sbjct: 243 HAGLFIIRYHSFYPLHKAGAYTHLMNDEDRDDLKWLHVFNKYDLYSKSKVLVDVEQVKPY 302

Query: 274 YMSLIEKYFPEKLKW 230
           Y+SLI KYFP KLKW
Sbjct: 303 YISLINKYFPAKLKW 317



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>MIOX_BRARE (Q4V8T0) Inositol oxygenase (EC 1.13.99.1) (Myo-inositol oxygenase)|
          Length = 278

 Score =  164 bits (416), Expect = 2e-40
 Identities = 72/122 (59%), Positives = 92/122 (75%)
 Frame = -2

Query: 595 NPDYHNPEFNTKFGVYSEGCGLDNVLMSWGHDDYMYLVAKENKTTLPSAGLFIIRYHSFY 416
           NPD  NP  NT+FG+Y   CGLD VLMSWGHD+Y+Y V K NK T+P  GL++IR+HSFY
Sbjct: 157 NPDGKNPAPNTEFGIYEPQCGLDKVLMSWGHDEYLYRVMKFNKCTIPEEGLYMIRFHSFY 216

Query: 415 PLHKHGAYTHLMNEEDKENLKWLHVFNKYDLYSKSSVRIDVEKVKPYYMSLIEKYFPEKL 236
           P H +G Y HL NE+D++ L W+  FNK+DLY+KS+   DVE++KPYY SLI+KY P  L
Sbjct: 217 PWHSNGDYMHLCNEKDQQMLPWVKEFNKFDLYTKSTELPDVERLKPYYQSLIDKYCPGVL 276

Query: 235 KW 230
           +W
Sbjct: 277 QW 278



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>MIOX_PIG (Q8WN98) Inositol oxygenase (EC 1.13.99.1) (Myo-inositol oxygenase)|
           (Aldehyde reductase-like 6)
          Length = 282

 Score =  145 bits (366), Expect = 1e-34
 Identities = 63/124 (50%), Positives = 85/124 (68%)
 Frame = -2

Query: 601 KENPDYHNPEFNTKFGVYSEGCGLDNVLMSWGHDDYMYLVAKENKTTLPSAGLFIIRYHS 422
           ++NPD  +P ++T+ G+Y   CGL+N LMSWGHD+YMY + K NK +LP    +IIR+HS
Sbjct: 159 QDNPDLQDPVYSTELGMYQPHCGLENALMSWGHDEYMYQMMKFNKFSLPGEAFYIIRFHS 218

Query: 421 FYPLHKHGAYTHLMNEEDKENLKWLHVFNKYDLYSKSSVRIDVEKVKPYYMSLIEKYFPE 242
           FYP H  G Y  L NE+D   L W+  FNK+DLY+K S   DV++++PYY  LI+KY P 
Sbjct: 219 FYPWHTGGDYRQLCNEQDLAMLPWVQEFNKFDLYTKGSDMPDVDELRPYYQGLIDKYCPG 278

Query: 241 KLKW 230
            L W
Sbjct: 279 VLCW 282



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>MIOX_MOUSE (Q9QXN5) Inositol oxygenase (EC 1.13.99.1) (Myo-inositol oxygenase)|
           (Aldehyde reductase-like 6) (Renal-specific
           oxidoreductase)
          Length = 285

 Score =  144 bits (364), Expect = 2e-34
 Identities = 61/124 (49%), Positives = 86/124 (69%)
 Frame = -2

Query: 601 KENPDYHNPEFNTKFGVYSEGCGLDNVLMSWGHDDYMYLVAKENKTTLPSAGLFIIRYHS 422
           ++NPD  +P ++T+ G+Y   CGL+NVLMSWGHD+Y+Y + K NK +LPS   ++IR+HS
Sbjct: 162 QDNPDLQDPRYSTELGMYQPHCGLENVLMSWGHDEYLYQMMKFNKFSLPSEAFYMIRFHS 221

Query: 421 FYPLHKHGAYTHLMNEEDKENLKWLHVFNKYDLYSKSSVRIDVEKVKPYYMSLIEKYFPE 242
           FYP H  G Y  L +++D + L W+  FNK+DLY+K     DVE ++PYY  LI+KY P 
Sbjct: 222 FYPWHTGGDYRQLCSQQDLDMLPWVQEFNKFDLYTKCPDLPDVESLRPYYQGLIDKYCPG 281

Query: 241 KLKW 230
            L W
Sbjct: 282 TLSW 285



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>MIOX_PONPY (Q5REY9) Inositol oxygenase (EC 1.13.99.1) (Myo-inositol oxygenase)|
          Length = 285

 Score =  140 bits (354), Expect = 2e-33
 Identities = 61/124 (49%), Positives = 83/124 (66%)
 Frame = -2

Query: 601 KENPDYHNPEFNTKFGVYSEGCGLDNVLMSWGHDDYMYLVAKENKTTLPSAGLFIIRYHS 422
           ++NPD  +P ++T+ G+Y   CGLD VLMSWGHD+YMY V K NK +LP    ++IR+HS
Sbjct: 162 QDNPDLQDPRYSTELGMYQPHCGLDRVLMSWGHDEYMYQVMKFNKFSLPPEAFYMIRFHS 221

Query: 421 FYPLHKHGAYTHLMNEEDKENLKWLHVFNKYDLYSKSSVRIDVEKVKPYYMSLIEKYFPE 242
           FYP H    Y  L +++D   L W+  FNK+DLY+K     DV+K++PYY  LI+KY P 
Sbjct: 222 FYPWHTGSDYQQLCSQQDLAMLPWVQEFNKFDLYTKCPDLPDVDKLRPYYQGLIDKYCPG 281

Query: 241 KLKW 230
            L W
Sbjct: 282 ILSW 285



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>MIOX_RAT (Q9QXN4) Inositol oxygenase (EC 1.13.99.1) (Myo-inositol oxygenase)|
           (Aldehyde reductase-like 6) (Renal-specific
           oxidoreductase) (Kidney-specific protein 32)
          Length = 285

 Score =  140 bits (353), Expect = 3e-33
 Identities = 58/124 (46%), Positives = 86/124 (69%)
 Frame = -2

Query: 601 KENPDYHNPEFNTKFGVYSEGCGLDNVLMSWGHDDYMYLVAKENKTTLPSAGLFIIRYHS 422
           ++NPD  +P ++T+ G+Y   CGL+NVLMSWGHD+Y+Y + K NK +LPS   +++R+HS
Sbjct: 162 QDNPDLQDPRYSTELGMYQPHCGLENVLMSWGHDEYLYQMMKFNKFSLPSEAFYMVRFHS 221

Query: 421 FYPLHKHGAYTHLMNEEDKENLKWLHVFNKYDLYSKSSVRIDVEKVKPYYMSLIEKYFPE 242
           FYP H  G Y  L +++D + L W+  FNK+DLY+K     +V+ ++PYY  LI+KY P 
Sbjct: 222 FYPWHTGGDYRQLCSQQDLDMLPWVQEFNKFDLYTKCPDLPEVKSLRPYYQGLIDKYCPG 281

Query: 241 KLKW 230
            L W
Sbjct: 282 ILSW 285



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>MIOX_HUMAN (Q9UGB7) Inositol oxygenase (EC 1.13.99.1) (Myo-inositol oxygenase)|
           (Aldehyde reductase-like 6) (Renal-specific
           oxidoreductase) (Kidney-specific protein 32)
          Length = 285

 Score =  140 bits (352), Expect = 4e-33
 Identities = 61/124 (49%), Positives = 83/124 (66%)
 Frame = -2

Query: 601 KENPDYHNPEFNTKFGVYSEGCGLDNVLMSWGHDDYMYLVAKENKTTLPSAGLFIIRYHS 422
           ++NPD  +P ++T+ G+Y   CGLD VLMSWGHD+YMY V K NK +LP    ++IR+HS
Sbjct: 162 QDNPDLQDPRYSTELGMYQPHCGLDRVLMSWGHDEYMYQVMKFNKFSLPPEAFYMIRFHS 221

Query: 421 FYPLHKHGAYTHLMNEEDKENLKWLHVFNKYDLYSKSSVRIDVEKVKPYYMSLIEKYFPE 242
           FYP H    Y  L +++D   L W+  FNK+DLY+K     DV+K++PYY  LI+KY P 
Sbjct: 222 FYPWHTGRDYQQLCSQQDLAMLPWVREFNKFDLYTKCPDLPDVDKLRPYYQGLIDKYCPG 281

Query: 241 KLKW 230
            L W
Sbjct: 282 ILSW 285



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>CSK_CHICK (P41239) Tyrosine-protein kinase CSK (EC 2.7.10.2) (C-SRC kinase)|
          Length = 450

 Score = 32.3 bits (72), Expect = 1.2
 Identities = 30/98 (30%), Positives = 42/98 (42%), Gaps = 3/98 (3%)
 Frame = -2

Query: 535 GLDNVLMSWGHDDYMYLVAKENKTTL---PSAGLFIIRYHSFYPLHKHGAYTHLMNEEDK 365
           G+   LM W H      + +E    L   P  GLF++R  + YP    G YT  ++ E K
Sbjct: 74  GIKLSLMPWFHGK----ITREQAERLLYPPETGLFLVRESTNYP----GDYTLCVSCEGK 125

Query: 364 ENLKWLHVFNKYDLYSKSSVRIDVEKVKPYYMSLIEKY 251
                  V +   +YS S + ID E      M L+E Y
Sbjct: 126 -------VEHYRIIYSSSKLSIDEEVYFENLMQLVEHY 156



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>SPB1_CRYNE (Q5KM86) AdoMet-dependent rRNA methyltransferase SPB1 (EC 2.1.1.-)|
           (2'-O-ribose RNA methyltransferase)
           (S-adenosyl-L-methionine-dependent methyltransferase)
          Length = 908

 Score = 32.3 bits (72), Expect = 1.2
 Identities = 20/64 (31%), Positives = 29/64 (45%)
 Frame = -2

Query: 433 RYHSFYPLHKHGAYTHLMNEEDKENLKWLHVFNKYDLYSKSSVRIDVEKVKPYYMSLIEK 254
           R   FY L K   Y        +   K +H+  KYDL SK+   ID+      ++ + EK
Sbjct: 12  RLDKFYRLAKEQGY------RARSAFKLVHLNRKYDLLSKARCCIDLCAAPGGWLQVAEK 65

Query: 253 YFPE 242
           Y P+
Sbjct: 66  YMPK 69



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>TOP1_RICPR (Q9ZDK2) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I)|
           (Omega-protein) (Relaxing enzyme) (Untwisting enzyme)
           (Swivelase)
          Length = 776

 Score = 30.4 bits (67), Expect = 4.6
 Identities = 21/64 (32%), Positives = 33/64 (51%)
 Frame = -2

Query: 538 CGLDNVLMSWGHDDYMYLVAKENKTTLPSAGLFIIRYHSFYPLHKHGAYTHLMNEEDKEN 359
           C ++NV+M    D  +  +A ENK  L  A   II +  FY +     Y   +++ED+E+
Sbjct: 388 CQMENVIM----DLVIANLASENKEYLAKANGSIIAFDGFYKV-----YRESLDDEDEED 438

Query: 358 LKWL 347
            K L
Sbjct: 439 NKML 442



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>GRPE_THEMA (Q9WZV4) Protein grpE (HSP-70 cofactor)|
          Length = 172

 Score = 30.0 bits (66), Expect = 6.1
 Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
 Frame = -2

Query: 394 YTHLMNEEDKENLKWLHVFNKYDLYSKSSV-RIDVEKVKPY 275
           Y  L+N  +KE L  +HV  K+D +   +V R++ E V+ Y
Sbjct: 88  YKKLLNVLEKEGLTKIHVGEKFDPFEHEAVERVETEDVEEY 128



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>RIN2_HUMAN (Q8WYP3) Ras and Rab interactor 2 (Ras interaction/interference|
           protein 2) (Ras inhibitor JC265) (Ras association domain
           family 4)
          Length = 895

 Score = 30.0 bits (66), Expect = 6.1
 Identities = 16/45 (35%), Positives = 28/45 (62%), Gaps = 2/45 (4%)
 Frame = -2

Query: 367 KENLKWLHVFNKYDL--YSKSSVRIDVEKVKPYYMSLIEKYFPEK 239
           KENL+ +   N  +L  ++ +   +DVEK+K  +M++ + Y PEK
Sbjct: 625 KENLQLVRQRNPQELGVFAPTPDFVDVEKIKVKFMTMQKMYSPEK 669


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,118,236
Number of Sequences: 219361
Number of extensions: 1779228
Number of successful extensions: 4789
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 4647
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4789
length of database: 80,573,946
effective HSP length: 107
effective length of database: 57,102,319
effective search space used: 5995743495
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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