ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd14m11
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1TL29_LYCES (Q9THX6) Putative L-ascorbate peroxidase, chloroplast... 243 4e-67
2TL29_ARATH (P82281) Putative L-ascorbate peroxidase, chloroplast... 235 1e-63
3UT023_PEA (P82338) Unknown protein from 2D-PAGE of thylakoid (Sp... 44 6e-04
4YP141_YEAST (Q03002) Putative serine/threonine-protein kinase YP... 32 2.4
5SYA_TROWT (Q83GD1) Alanyl-tRNA synthetase (EC 6.1.1.7) (Alanine-... 32 2.4
6SYA_TROW8 (Q83HV0) Alanyl-tRNA synthetase (EC 6.1.1.7) (Alanine-... 32 2.4
7APX1_ARATH (Q05431) L-ascorbate peroxidase, cytosolic (EC 1.11.1... 32 2.4
8TAB2_MOUSE (Q99K90) Mitogen-activated protein kinase kinase kina... 31 3.2
9APX1_PEA (P48534) L-ascorbate peroxidase, cytosolic (EC 1.11.1.1... 31 3.2
10PP71_HCMVA (P06726) 71 kDa upper matrix phosphoprotein (PP71) (T... 31 4.2
11C13A2_CAEEL (Q27518) Putative cytochrome P450 CYP13A2 (EC 1.14.-.-) 31 4.2
12EUTP_ECOLI (P76556) Ethanolamine utilization protein eutP 30 5.4
13GPMI_PROMA (Q7VA78) 2,3-bisphosphoglycerate-independent phosphog... 30 5.4
14BTUB_YERPE (Q8ZAA1) Vitamin B12 transporter btuB precursor (Coba... 30 5.4
15CCPR2_CRYNE (Q5KGE6) Putative heme-binding peroxidase (EC 1.11.1.-) 30 7.1
16TAB2_RAT (Q5U303) Mitogen-activated protein kinase kinase kinase... 30 9.3

>TL29_LYCES (Q9THX6) Putative L-ascorbate peroxidase, chloroplast precursor (EC|
           1.11.1.11) (Thylakoid lumenal 29 kDa protein) (TL29)
           (P29)
          Length = 345

 Score =  243 bits (620), Expect(2) = 4e-67
 Identities = 118/152 (77%), Positives = 131/152 (86%)
 Frame = -2

Query: 632 AAIAKTGGNQEKGRTLYSAYGSSGQWGFFDKIFGRDDVQEPDPEGRVPQWSTASVQEMKD 453
           +AI+K GGN EKG  LYSAYGS+GQWG FD+IFGR D QEPDPEGRVPQW  ASVQEMKD
Sbjct: 194 SAISKCGGNVEKGTLLYSAYGSNGQWGQFDRIFGRSDAQEPDPEGRVPQWDKASVQEMKD 253

Query: 452 KFISVGLGPRQVAVMSAFFGPDQAATEAKLIVDPDCRPWVEKYQRSRETVSRTDYEVDLI 273
           KF +VGLGPRQ+AVMS+F GPDQAATEA L  DP+  PW++KYQRSRETVSRTDYEVDLI
Sbjct: 254 KFKAVGLGPRQLAVMSSFLGPDQAATEALLASDPEVLPWIQKYQRSRETVSRTDYEVDLI 313

Query: 272 TTVTKLSYLGQKINYEAYTYPKQKINLGNLKL 177
           TTVTKLS LGQ INYEAYTYP +KI++  LKL
Sbjct: 314 TTVTKLSSLGQVINYEAYTYPPRKIDVTKLKL 345



 Score = 32.0 bits (71), Expect(2) = 4e-67
 Identities = 13/21 (61%), Positives = 16/21 (76%)
 Frame = -1

Query: 702 GPISFADLVQIGASQALKKTF 640
           GPIS+ADL+Q  A  A+K TF
Sbjct: 171 GPISYADLIQFAAQSAVKSTF 191



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>TL29_ARATH (P82281) Putative L-ascorbate peroxidase, chloroplast precursor (EC|
           1.11.1.11) (Thylakoid lumenal 29 kDa protein) (TL29)
           (P29)
          Length = 349

 Score =  235 bits (600), Expect(2) = 1e-63
 Identities = 113/154 (73%), Positives = 127/154 (82%)
 Frame = -2

Query: 638 LDAAIAKTGGNQEKGRTLYSAYGSSGQWGFFDKIFGRDDVQEPDPEGRVPQWSTASVQEM 459
           L +AI K GGN+EKG  LY+AYGS+GQWG FD+ FGR D  E DPEGRVPQW  A+VQEM
Sbjct: 196 LASAIRKCGGNEEKGNLLYTAYGSAGQWGLFDRNFGRSDATEADPEGRVPQWGKATVQEM 255

Query: 458 KDKFISVGLGPRQVAVMSAFFGPDQAATEAKLIVDPDCRPWVEKYQRSRETVSRTDYEVD 279
           KDKFI+VGLGPRQ+AVMSAF GPDQAATE  L  DP   PWV+KYQRSRETVS+TDYEVD
Sbjct: 256 KDKFIAVGLGPRQLAVMSAFLGPDQAATEQLLATDPQVAPWVQKYQRSRETVSQTDYEVD 315

Query: 278 LITTVTKLSYLGQKINYEAYTYPKQKINLGNLKL 177
           LIT  TKLS LGQ+IN+EAYTYP ++INL  LKL
Sbjct: 316 LITAFTKLSCLGQQINFEAYTYPVERINLSKLKL 349



 Score = 28.1 bits (61), Expect(2) = 1e-63
 Identities = 10/21 (47%), Positives = 16/21 (76%)
 Frame = -1

Query: 702 GPISFADLVQIGASQALKKTF 640
           GPIS+AD++Q+    A+K T+
Sbjct: 175 GPISYADIIQLAGQSAVKFTY 195



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>UT023_PEA (P82338) Unknown protein from 2D-PAGE of thylakoid (Spot 23) (Spot|
           28) (Spot 205) (Fragments)
          Length = 45

 Score = 43.5 bits (101), Expect = 6e-04
 Identities = 25/49 (51%), Positives = 30/49 (61%), Gaps = 6/49 (12%)
 Frame = -2

Query: 335 VEKYQRSRETVS------RTDYEVDLITTVTKLSYLGQKINYEAYTYPK 207
           +E+ QRS    S      RTDYEVD++TT TKL       NYEAYTYP+
Sbjct: 4   IERRQRSEFQFSAVGLGPRTDYEVDILTTFTKL-------NYEAYTYPR 45



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>YP141_YEAST (Q03002) Putative serine/threonine-protein kinase YPL141C (EC|
           2.7.11.1)
          Length = 865

 Score = 31.6 bits (70), Expect = 2.4
 Identities = 16/53 (30%), Positives = 28/53 (52%), Gaps = 2/53 (3%)
 Frame = +3

Query: 21  FSKNTQRHSQNMNLSSQMKPAP--RRHPTVPDGWSSQFSNDAIAHRNTQSNHQ 173
           +S +T + S   ++ S+ KP+P  +R P   D + +   N  I   N Q++HQ
Sbjct: 746 YSYDTSKSSLYSSMDSKRKPSPPSQRRPKKDDSYQTNSKNHYITASNMQTSHQ 798



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>SYA_TROWT (Q83GD1) Alanyl-tRNA synthetase (EC 6.1.1.7) (Alanine--tRNA ligase)|
           (AlaRS)
          Length = 880

 Score = 31.6 bits (70), Expect = 2.4
 Identities = 26/106 (24%), Positives = 47/106 (44%), Gaps = 19/106 (17%)
 Frame = -2

Query: 614 GGNQEKGRTLYSAYGSSGQWG--------FFDKIFGRDDVQEPDPEGRVPQWSTASVQEM 459
           G  QE+ ++L S + S+G+ G        F+D   G     E DP+  +  W+   +Q +
Sbjct: 138 GVPQERVQSLDSNFWSTGKAGPAGPCSEVFYDLHGGPGPGPEGDPDRYLEVWNLVFMQNL 197

Query: 458 KD-----------KFISVGLGPRQVAVMSAFFGPDQAATEAKLIVD 354
           +D           K +  G+G  ++A+++   G      E K I+D
Sbjct: 198 RDNDGHIISSLPKKCVDTGMGLERIAMITQGVGTIYDTDELKPILD 243



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>SYA_TROW8 (Q83HV0) Alanyl-tRNA synthetase (EC 6.1.1.7) (Alanine--tRNA ligase)|
           (AlaRS)
          Length = 880

 Score = 31.6 bits (70), Expect = 2.4
 Identities = 26/106 (24%), Positives = 47/106 (44%), Gaps = 19/106 (17%)
 Frame = -2

Query: 614 GGNQEKGRTLYSAYGSSGQWG--------FFDKIFGRDDVQEPDPEGRVPQWSTASVQEM 459
           G  QE+ ++L S + S+G+ G        F+D   G     E DP+  +  W+   +Q +
Sbjct: 138 GVPQERVQSLDSNFWSTGKAGPAGPCSEVFYDLHGGPGPGPEGDPDRYLEVWNLVFMQNL 197

Query: 458 KD-----------KFISVGLGPRQVAVMSAFFGPDQAATEAKLIVD 354
           +D           K +  G+G  ++A+++   G      E K I+D
Sbjct: 198 RDNDGHIISSLPKKCVDTGMGLERIAMITQGVGTIYDTDELKPILD 243



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>APX1_ARATH (Q05431) L-ascorbate peroxidase, cytosolic (EC 1.11.1.11) (AP)|
          Length = 249

 Score = 31.6 bits (70), Expect = 2.4
 Identities = 33/135 (24%), Positives = 50/135 (37%), Gaps = 38/135 (28%)
 Frame = -2

Query: 533 GRDDVQEPDPEGRVPQWSTASVQEMKDKFI-SVGLGPRQVAVMS---------------- 405
           GR+D  +P PEGR+P  +T     ++D F   +GL  + +  +S                
Sbjct: 117 GREDKPQPPPEGRLPD-ATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGRCHKDRSGFE 175

Query: 404 ------------AFF---------GPDQAATEAKLIVDPDCRPWVEKYQRSRETVSRTDY 288
                       ++F         G  Q  ++  L+ DP  RP VEKY    +      +
Sbjct: 176 GAWTSNPLIFDNSYFKELLSGEKEGLLQLVSDKALLDDPVFRPLVEKYAADEDA-----F 230

Query: 287 EVDLITTVTKLSYLG 243
             D      KLS LG
Sbjct: 231 FADYAEAHMKLSELG 245



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>TAB2_MOUSE (Q99K90) Mitogen-activated protein kinase kinase kinase|
           7-interacting protein 2 (TAK1-binding protein 2) (TAB2)
          Length = 693

 Score = 31.2 bits (69), Expect = 3.2
 Identities = 14/56 (25%), Positives = 30/56 (53%)
 Frame = +3

Query: 24  SKNTQRHSQNMNLSSQMKPAPRRHPTVPDGWSSQFSNDAIAHRNTQSNHQSQLQVT 191
           ++ TQ+HS  ++  + M P     P+ P  W++  +++ + H +TQ  +Q   Q +
Sbjct: 226 ARQTQQHSGWVSQFNPMNPQQAYQPSQPGPWTTYPASNPLPHTSTQQPNQQGHQTS 281



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>APX1_PEA (P48534) L-ascorbate peroxidase, cytosolic (EC 1.11.1.11) (AP)|
          Length = 249

 Score = 31.2 bits (69), Expect = 3.2
 Identities = 16/44 (36%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
 Frame = -2

Query: 533 GRDDVQEPDPEGRVPQWSTASVQEMKDKF-ISVGLGPRQVAVMS 405
           GR+D  EP PEGR+P  +T     ++D F  ++GL  + +  +S
Sbjct: 117 GREDKPEPPPEGRLPD-ATKGSDHLRDVFGKAMGLSDQDIVALS 159



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>PP71_HCMVA (P06726) 71 kDa upper matrix phosphoprotein (PP71) (Tegument|
           protein UL82)
          Length = 559

 Score = 30.8 bits (68), Expect = 4.2
 Identities = 14/38 (36%), Positives = 17/38 (44%), Gaps = 5/38 (13%)
 Frame = +2

Query: 491 APCPPDQALAH-----HLARIFCRRNPTAHLNRMPSTG 589
           +P PP     H     H A +  R NP  HL R+P  G
Sbjct: 245 SPHPPTSVFLHFSVYTHRAEVVARHNPYPHLRRLPDNG 282



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>C13A2_CAEEL (Q27518) Putative cytochrome P450 CYP13A2 (EC 1.14.-.-)|
          Length = 515

 Score = 30.8 bits (68), Expect = 4.2
 Identities = 17/43 (39%), Positives = 23/43 (53%)
 Frame = -2

Query: 548 FDKIFGRDDVQEPDPEGRVPQWSTASVQEMKDKFISVGLGPRQ 420
           +D     +DV+E  PE    +WST    E K  ++  GLGPRQ
Sbjct: 421 YDPELWGEDVKEFKPE----RWSTDEPLEHKGAYLPFGLGPRQ 459



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>EUTP_ECOLI (P76556) Ethanolamine utilization protein eutP|
          Length = 159

 Score = 30.4 bits (67), Expect = 5.4
 Identities = 26/96 (27%), Positives = 42/96 (43%), Gaps = 2/96 (2%)
 Frame = -2

Query: 509 DPEGRVPQWSTASVQEMKDKFISVGLGPRQVAVMSAFFGPDQ--AATEAKLIVDPDCRPW 336
           DPE R+P              + +G+  RQ+AV+S    PD   AAT   L+      P 
Sbjct: 76  DPESRLPAG-----------LLDIGVSKRQIAVISKTDMPDADVAATRKLLLETGFEEPM 124

Query: 335 VEKYQRSRETVSRTDYEVDLITTVTKLSYLGQKINY 228
            E      ++V +    VD + ++TK    G+K ++
Sbjct: 125 FELNSHDPQSVQQL---VDYLASLTKQEEAGEKTHH 157



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>GPMI_PROMA (Q7VA78) 2,3-bisphosphoglycerate-independent phosphoglycerate|
           mutase (EC 5.4.2.1) (Phosphoglyceromutase)
           (BPG-independent PGAM) (iPGM)
          Length = 540

 Score = 30.4 bits (67), Expect = 5.4
 Identities = 17/45 (37%), Positives = 22/45 (48%)
 Frame = -2

Query: 398 FGPDQAATEAKLIVDPDCRPWVEKYQRSRETVSRTDYEVDLITTV 264
           F PD+A    K I  P    +  KYQ     V+ T YE+DL  +V
Sbjct: 267 FRPDRARQLIKSITLPSFSEFKRKYQPQLNVVTLTQYEMDLPVSV 311



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>BTUB_YERPE (Q8ZAA1) Vitamin B12 transporter btuB precursor (Cobalamin|
           receptor) (Outer membrane cobalamin translocator)
          Length = 625

 Score = 30.4 bits (67), Expect = 5.4
 Identities = 26/91 (28%), Positives = 43/91 (47%), Gaps = 2/91 (2%)
 Frame = -2

Query: 443 SVGLGPRQVAVMSAFFGPDQAATEAKLIVDPDCRPWVEKYQRSRETVS-RTDYEV-DLIT 270
           S   GP    V   +  P  A T   L+            +R+++ V  + D++V DL  
Sbjct: 500 SFDTGPLSHQVTLEYLDPRNADTHEILV------------RRAKQQVKYQLDWQVADLDW 547

Query: 269 TVTKLSYLGQKINYEAYTYPKQKINLGNLKL 177
           +VT   YLGQ+ + +  TYP++ + LG + L
Sbjct: 548 SVT-YQYLGQRYDKDYSTYPEETVELGGVSL 577



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>CCPR2_CRYNE (Q5KGE6) Putative heme-binding peroxidase (EC 1.11.1.-)|
          Length = 315

 Score = 30.0 bits (66), Expect = 7.1
 Identities = 12/18 (66%), Positives = 13/18 (72%)
 Frame = -2

Query: 377 TEAKLIVDPDCRPWVEKY 324
           T+  LI DP  RPWVEKY
Sbjct: 211 TDMALIEDPSFRPWVEKY 228



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>TAB2_RAT (Q5U303) Mitogen-activated protein kinase kinase kinase|
           7-interacting protein 2
          Length = 693

 Score = 29.6 bits (65), Expect = 9.3
 Identities = 13/56 (23%), Positives = 30/56 (53%)
 Frame = +3

Query: 24  SKNTQRHSQNMNLSSQMKPAPRRHPTVPDGWSSQFSNDAIAHRNTQSNHQSQLQVT 191
           ++ TQ+HS  ++  + + P     P+ P  W++  +++ + H +TQ  +Q   Q +
Sbjct: 226 ARQTQQHSGWVSQFNPVNPQQAYQPSQPGPWTTYPASNPLPHTSTQQPNQQGHQTS 281


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 111,014,689
Number of Sequences: 219361
Number of extensions: 2495847
Number of successful extensions: 7262
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 6978
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7260
length of database: 80,573,946
effective HSP length: 109
effective length of database: 56,663,597
effective search space used: 7026286028
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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