>SAP_HUMAN (P07602) Proactivator polypeptide precursor [Contains: Saposin A|
(Protein A); Saposin B-Val; Saposin B (Sphingolipid
activator protein 1) (SAP-1) (Cerebroside sulfate
activator) (CSAct) (Dispersin) (Sulfatide/GM1
activator); Saposin C (Co-beta-
Length = 524
Score = 52.4 bits (124), Expect = 1e-06
Identities = 33/138 (23%), Positives = 69/138 (50%), Gaps = 8/138 (5%)
Frame = -3
Query: 635 KKCVELVDSY-ATLLFAKIAEIXPEEFCKRNGLCRXNAL------LSGVRSESTCVFCHH 477
++C E+VD+Y +++L + E+ PE C LC L ++ + C C
Sbjct: 355 EECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGGFCEVCKK 414
Query: 476 LMDEVLSKLKDPDAEFEIIQILLKECXKI-EGHEQQCKRLVLQYIPLILVNGXKFLEKND 300
L+ + L+ + EI+ L K C + + +++QC + V +Y P+++ + ++ +
Sbjct: 415 LVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSF 474
Query: 299 ICTIVQACDAGKKXVLGS 246
+C + AC + K +LG+
Sbjct: 475 VCLKIGACPSAHKPLLGT 492
Score = 35.8 bits (81), Expect = 0.11
Identities = 26/108 (24%), Positives = 48/108 (44%), Gaps = 3/108 (2%)
Frame = -3
Query: 494 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECXKI--EGHEQQCKRLVLQYIPLIL-VNG 324
C C ++ LKD E EI+ L K C + CK +V Y+P+IL +
Sbjct: 63 CDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIK 122
Query: 323 XKFLEKNDICTIVQACDAGKKXVLGSFSEEGLLXDE*RQKDYTEPRKP 180
+ ++C+ + C++ +K + ++ L ++ + D TE P
Sbjct: 123 GEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIPELDMTEVVAP 170
>SAP_BOVIN (P26779) Proactivator polypeptide precursor [Contains: Saposin A|
(Protein A); Saposin B (Sphingolipid activator protein
1) (SAP-1) (Cerebroside sulfate activator) (CSAct)
(Dispersin) (Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A
Length = 525
Score = 50.1 bits (118), Expect = 6e-06
Identities = 33/138 (23%), Positives = 65/138 (47%), Gaps = 8/138 (5%)
Frame = -3
Query: 635 KKCVELVDSYA-TLLFAKIAEIXPEEFCKRNGLCRXNALLSGV------RSESTCVFCHH 477
++C E+VD+Y ++L + E PE C LC L + + C C
Sbjct: 356 EQCQEVVDTYGRSILSILLDEASPELVCSMLHLCSSRGLPAATVRVMPRKDGGFCEVCKK 415
Query: 476 LMDEVLSKLKDPDAEFEIIQILLKECXKI-EGHEQQCKRLVLQYIPLILVNGXKFLEKND 300
L+ + L+ + +I+ L K C + + + +QC + V +Y P+++ + ++ +
Sbjct: 416 LVGYLDRNLEKNSTKEQILAALEKGCSFLPDQYRKQCDQFVTEYEPVLIEILVEVMDPSF 475
Query: 299 ICTIVQACDAGKKXVLGS 246
+C + AC A K +LG+
Sbjct: 476 VCLKIGACPAAHKPLLGA 493
Score = 33.5 bits (75), Expect = 0.54
Identities = 25/109 (22%), Positives = 49/109 (44%), Gaps = 4/109 (3%)
Frame = -3
Query: 494 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECXKIE--GHEQQCKRLVLQYIPLIL--VN 327
C C ++ + LKD E EI+ L + C + CK +V Y+P+IL +
Sbjct: 63 CDICKDVITAAGNLLKDNATEQEILMYLERTCDWLPKPNMSASCKEIVDSYLPVILDMIK 122
Query: 326 GXKFLEKNDICTIVQACDAGKKXVLGSFSEEGLLXDE*RQKDYTEPRKP 180
G + ++C+ + C++ +K + ++ L ++ + D E P
Sbjct: 123 G-QMSHPGEVCSALNLCESLQKHLAELNHQKQLESNQIPELDMAEVVAP 170
>SAP_CHICK (O13035) Proactivator polypeptide precursor [Contains: Saposin A;|
Saposin B; Saposin C; Saposin D]
Length = 518
Score = 42.7 bits (99), Expect = 9e-04
Identities = 30/123 (24%), Positives = 56/123 (45%), Gaps = 3/123 (2%)
Frame = -3
Query: 539 CRXNALLSGVRSESTCVFCHHLMDEVLSKLKDPDAEFEIIQILLKECXKI--EGHEQQCK 366
C+ N + C C L+ V LKD E EI L K C + +G +CK
Sbjct: 49 CQQNVWSKPAVNSIPCDLCKELVTVVGKVLKDNGTEDEIRSYLEKRCEFLPDQGLASECK 108
Query: 365 RLVLQYIPLIL-VNGXKFLEKNDICTIVQACDAGKKXVLGSFSEEGLLXDE*RQKDYTEP 189
+V Y+P+I+ + +F + +C+ + C + +K + ++ L ++ + D++E
Sbjct: 109 EIVDSYLPVIMDMIKEEFDKPEVVCSALSLCQSLQKHLAAMKLQKQLQSNKIPELDFSEL 168
Query: 188 RKP 180
P
Sbjct: 169 TSP 171
Score = 39.3 bits (90), Expect = 0.010
Identities = 32/134 (23%), Positives = 55/134 (41%), Gaps = 6/134 (4%)
Frame = -3
Query: 632 KCVELVDSYATLLFAKIAEIX-PEEFCKRNGLCRXNAL----LSGVRSESTCVFCHHLMD 468
+C + ++ Y L + E PE C C N + + C C ++
Sbjct: 352 QCKDFIEVYGQALIDMLLEATNPEAVCVMLKCCAANKPPQQPVVVKPAGGFCDICKMIVA 411
Query: 467 EVLSKLKDPDAEFEIIQILLKECXKI-EGHEQQCKRLVLQYIPLILVNGXKFLEKNDICT 291
+L+ EI +L K C + E QC + V QY P+++ + ++ +CT
Sbjct: 412 YADKELEKNATTTEIEALLEKVCHFLPESVSDQCVQFVEQYEPVVVQLLAEMMDPTFVCT 471
Query: 290 IVQACDAGKKXVLG 249
+ C A KK +LG
Sbjct: 472 KLGVCGAAKKPLLG 485
Score = 29.6 bits (65), Expect = 7.9
Identities = 18/80 (22%), Positives = 33/80 (41%), Gaps = 1/80 (1%)
Frame = -3
Query: 500 STCVFCHHLMDEVLSKLKDPDAEFEIIQILLKECXKIEGH-EQQCKRLVLQYIPLILVNG 324
S C C ++ EV L+ E EI+ + C + + QCK + Y ++
Sbjct: 309 SVCEICETMVKEVTGLLESNKTEEEIVHEMEVVCYLLPASVKDQCKDFIEVYGQALIDML 368
Query: 323 XKFLEKNDICTIVQACDAGK 264
+ +C +++ C A K
Sbjct: 369 LEATNPEAVCVMLKCCAANK 388
>SAP_MOUSE (Q61207) Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)|
Length = 557
Score = 40.8 bits (94), Expect = 0.003
Identities = 38/168 (22%), Positives = 67/168 (39%), Gaps = 39/168 (23%)
Frame = -3
Query: 632 KCVELVDSYA-TLLFAKIAEIXPEEFCKRNGLCRXN------------ALLSGVRSEST- 495
KC E+V ++ +LL I E+ P C GLC A++S + E T
Sbjct: 358 KCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTP 417
Query: 494 ------------------------CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECXKI- 390
C C L+ + L+ + EI+ L K C +
Sbjct: 418 PKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLP 477
Query: 389 EGHEQQCKRLVLQYIPLILVNGXKFLEKNDICTIVQACDAGKKXVLGS 246
+ +++QC V +Y PL+L + ++ +C+ + C + K +LG+
Sbjct: 478 DPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGT 525
Score = 34.3 bits (77), Expect = 0.32
Identities = 22/79 (27%), Positives = 37/79 (46%), Gaps = 4/79 (5%)
Frame = -3
Query: 494 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECXKIEGH--EQQCKRLVLQYIPLIL--VN 327
C C ++ E + LKD + EI+ L K C I CK +V Y+P+IL +
Sbjct: 63 CDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIK 122
Query: 326 GXKFLEKNDICTIVQACDA 270
G + ++C+ + C +
Sbjct: 123 G-EMSNPGEVCSALNLCQS 140
>SAP_RAT (P10960) Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)|
Length = 554
Score = 38.5 bits (88), Expect = 0.017
Identities = 21/84 (25%), Positives = 43/84 (51%), Gaps = 1/84 (1%)
Frame = -3
Query: 494 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECXKI-EGHEQQCKRLVLQYIPLILVNGXK 318
C C L+ + L+ + EI+ L K C + + +++QC V +Y PL+L +
Sbjct: 439 CEVCKKLVIYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEPLLLEILVE 498
Query: 317 FLEKNDICTIVQACDAGKKXVLGS 246
++ + +C+ + C + K +LG+
Sbjct: 499 VMDPSFVCSKIGVCPSAYKLLLGT 522
Score = 35.8 bits (81), Expect = 0.11
Identities = 23/79 (29%), Positives = 37/79 (46%), Gaps = 4/79 (5%)
Frame = -3
Query: 494 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECXKIEGH--EQQCKRLVLQYIPLIL--VN 327
C C ++ E + LKD E EI+ L K C I CK +V Y+P+IL +
Sbjct: 63 CDICKTVVTEAGNLLKDNATEEEILHYLEKTCAWIHDSSLSASCKEVVDSYLPVILDMIK 122
Query: 326 GXKFLEKNDICTIVQACDA 270
G + ++C+ + C +
Sbjct: 123 G-EMSNPGEVCSALNLCQS 140
>NF1_RAT (P97526) Neurofibromin (Neurofibromatosis-related protein NF-1)|
Length = 2820
Score = 29.6 bits (65), Expect = 7.9
Identities = 20/64 (31%), Positives = 31/64 (48%), Gaps = 5/64 (7%)
Frame = -3
Query: 479 HLMDEVLSKLKDPDAEFEII-----QILLKECXKIEGHEQQCKRLVLQYIPLILVNGXKF 315
H +D + L +PDA E Q+L C K+ H+ +L+++ IL+ KF
Sbjct: 553 HQLDSI--DLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKF 610
Query: 314 LEKN 303
L KN
Sbjct: 611 LLKN 614
Database: uniprot_sprot.fasta
Posted date: May 25, 2006 5:36 PM
Number of letters in database: 80,573,946
Number of sequences in database: 219,361
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,463,934
Number of Sequences: 219361
Number of extensions: 1609151
Number of successful extensions: 4134
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 3992
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4131
length of database: 80,573,946
effective HSP length: 107
effective length of database: 57,102,319
effective search space used: 5938641176
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)