ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd14l09
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1SAP_CHICK (O13035) Proactivator polypeptide precursor [Contains:... 45 1e-04
2SAP_HUMAN (P07602) Proactivator polypeptide precursor [Contains:... 43 7e-04
3SAP_BOVIN (P26779) Proactivator polypeptide precursor [Contains:... 41 0.003
4SAP_RAT (P10960) Sulfated glycoprotein 1 precursor (SGP-1) (Pros... 38 0.023
5SAP_MOUSE (Q61207) Sulfated glycoprotein 1 precursor (SGP-1) (Pr... 37 0.068
6OXAA_DEIRA (Q9RSH5) Membrane protein oxaA 34 0.44
7NF1_MOUSE (Q04690) Neurofibromin (Neurofibromatosis-related prot... 32 1.3
8NF1_HUMAN (P21359) Neurofibromin (Neurofibromatosis-related prot... 32 1.3
9NF1_RAT (P97526) Neurofibromin (Neurofibromatosis-related protei... 32 1.3
10SYP32_ARATH (Q9LK09) Syntaxin-32 (AtSYP32) 30 8.3
11SEN54_SCHPO (O74908) Probable tRNA-splicing endonuclease subunit... 30 8.3
12YGEZ_ECOLI (Q46806) Hypothetical hydrolase ygeZ (EC 3.-.-.-) 30 8.3
13GLRX1_YEAST (P25373) Glutaredoxin-1 (Glutathione-dependent oxido... 30 8.3

>SAP_CHICK (O13035) Proactivator polypeptide precursor [Contains: Saposin A;|
           Saposin B; Saposin C; Saposin D]
          Length = 518

 Score = 45.4 bits (106), Expect = 1e-04
 Identities = 31/123 (25%), Positives = 58/123 (47%), Gaps = 3/123 (2%)
 Frame = -2

Query: 604 CRDNALLSGVRSESTCVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKI--EGHEQQCK 431
           C+ N       +   C  C  L+  V   LKD   E EI   L K C+ +  +G   +CK
Sbjct: 49  CQQNVWSKPAVNSIPCDLCKELVTVVGKVLKDNGTEDEIRSYLEKRCEFLPDQGLASECK 108

Query: 430 RLVLQYIPLIL-VNGEKFLEKNDICTIVQACDAGKKTVLGSFSEEGLLRDE*RQKDYTEP 254
            +V  Y+P+I+ +  E+F +   +C+ +  C + +K +     ++ L  ++  + D++E 
Sbjct: 109 EIVDSYLPVIMDMIKEEFDKPEVVCSALSLCQSLQKHLAAMKLQKQLQSNKIPELDFSEL 168

Query: 253 RKP 245
             P
Sbjct: 169 TSP 171



 Score = 37.0 bits (84), Expect = 0.052
 Identities = 23/83 (27%), Positives = 39/83 (46%), Gaps = 1/83 (1%)
 Frame = -2

Query: 559 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKI-EGHEQQCKRLVLQYIPLILVNGEK 383
           C  C  ++     +L+      EI  +L K C  + E    QC + V QY P+++    +
Sbjct: 403 CDICKMIVAYADKELEKNATTTEIEALLEKVCHFLPESVSDQCVQFVEQYEPVVVQLLAE 462

Query: 382 FLEKNDICTIVQACDAGKKTVLG 314
            ++   +CT +  C A KK +LG
Sbjct: 463 MMDPTFVCTKLGVCGAAKKPLLG 485



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>SAP_HUMAN (P07602) Proactivator polypeptide precursor [Contains: Saposin A|
           (Protein A); Saposin B-Val; Saposin B (Sphingolipid
           activator protein 1) (SAP-1) (Cerebroside sulfate
           activator) (CSAct) (Dispersin) (Sulfatide/GM1
           activator); Saposin C (Co-beta-
          Length = 524

 Score = 43.1 bits (100), Expect = 7e-04
 Identities = 27/120 (22%), Positives = 56/120 (46%), Gaps = 7/120 (5%)
 Frame = -2

Query: 649 IAEIKPEEFCKRNGLCRDNAL------LSGVRSESTCVFCHHLMDEVLSKLKDPDAEFEI 488
           + E+ PE  C    LC    L      ++  +    C  C  L+  +   L+    + EI
Sbjct: 373 LEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEI 432

Query: 487 IQILLKECKKI-EGHEQQCKRLVLQYIPLILVNGEKFLEKNDICTIVQACDAGKKTVLGS 311
           +  L K C  + + +++QC + V +Y P+++    + ++ + +C  + AC +  K +LG+
Sbjct: 433 LAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGT 492



 Score = 36.2 bits (82), Expect = 0.089
 Identities = 28/109 (25%), Positives = 49/109 (44%), Gaps = 4/109 (3%)
 Frame = -2

Query: 559 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKI--EGHEQQCKRLVLQYIPLIL--VN 392
           C  C  ++      LKD   E EI+  L K C  +        CK +V  Y+P+IL  + 
Sbjct: 63  CDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIK 122

Query: 391 GEKFLEKNDICTIVQACDAGKKTVLGSFSEEGLLRDE*RQKDYTEPRKP 245
           GE      ++C+ +  C++ +K +     ++ L  ++  + D TE   P
Sbjct: 123 GE-MSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIPELDMTEVVAP 170



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>SAP_BOVIN (P26779) Proactivator polypeptide precursor [Contains: Saposin A|
           (Protein A); Saposin B (Sphingolipid activator protein
           1) (SAP-1) (Cerebroside sulfate activator) (CSAct)
           (Dispersin) (Sulfatide/GM1 activator); Saposin C
           (Co-beta-glucosidase) (A
          Length = 525

 Score = 41.2 bits (95), Expect = 0.003
 Identities = 27/118 (22%), Positives = 52/118 (44%), Gaps = 7/118 (5%)
 Frame = -2

Query: 643 EIKPEEFCKRNGLCRDNALLSGV------RSESTCVFCHHLMDEVLSKLKDPDAEFEIIQ 482
           E  PE  C    LC    L +        +    C  C  L+  +   L+    + +I+ 
Sbjct: 376 EASPELVCSMLHLCSSRGLPAATVRVMPRKDGGFCEVCKKLVGYLDRNLEKNSTKEQILA 435

Query: 481 ILLKECKKI-EGHEQQCKRLVLQYIPLILVNGEKFLEKNDICTIVQACDAGKKTVLGS 311
            L K C  + + + +QC + V +Y P+++    + ++ + +C  + AC A  K +LG+
Sbjct: 436 ALEKGCSFLPDQYRKQCDQFVTEYEPVLIEILVEVMDPSFVCLKIGACPAAHKPLLGA 493



 Score = 33.5 bits (75), Expect = 0.58
 Identities = 21/82 (25%), Positives = 39/82 (47%), Gaps = 4/82 (4%)
 Frame = -2

Query: 559 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKIE--GHEQQCKRLVLQYIPLIL--VN 392
           C  C  ++    + LKD   E EI+  L + C  +        CK +V  Y+P+IL  + 
Sbjct: 63  CDICKDVITAAGNLLKDNATEQEILMYLERTCDWLPKPNMSASCKEIVDSYLPVILDMIK 122

Query: 391 GEKFLEKNDICTIVQACDAGKK 326
           G+      ++C+ +  C++ +K
Sbjct: 123 GQ-MSHPGEVCSALNLCESLQK 143



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>SAP_RAT (P10960) Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)|
          Length = 554

 Score = 38.1 bits (87), Expect = 0.023
 Identities = 21/84 (25%), Positives = 43/84 (51%), Gaps = 1/84 (1%)
 Frame = -2

Query: 559 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKI-EGHEQQCKRLVLQYIPLILVNGEK 383
           C  C  L+  +   L+    + EI+  L K C  + + +++QC   V +Y PL+L    +
Sbjct: 439 CEVCKKLVIYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEPLLLEILVE 498

Query: 382 FLEKNDICTIVQACDAGKKTVLGS 311
            ++ + +C+ +  C +  K +LG+
Sbjct: 499 VMDPSFVCSKIGVCPSAYKLLLGT 522



 Score = 38.1 bits (87), Expect = 0.023
 Identities = 27/107 (25%), Positives = 44/107 (41%), Gaps = 4/107 (3%)
 Frame = -2

Query: 643 EIKPEEFCKRNGLCRDNALLSGVRSESTCVFCHHLMDEVLSKLKDPDAEFEIIQILLKEC 464
           ++K    C+    C+             C  C  ++ E  + LKD   E EI+  L K C
Sbjct: 35  DVKTAVDCRAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATEEEILHYLEKTC 94

Query: 463 KKIEGH--EQQCKRLVLQYIPLIL--VNGEKFLEKNDICTIVQACDA 335
             I        CK +V  Y+P+IL  + GE      ++C+ +  C +
Sbjct: 95  AWIHDSSLSASCKEVVDSYLPVILDMIKGE-MSNPGEVCSALNLCQS 140



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>SAP_MOUSE (Q61207) Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)|
          Length = 557

 Score = 36.6 bits (83), Expect = 0.068
 Identities = 21/84 (25%), Positives = 42/84 (50%), Gaps = 1/84 (1%)
 Frame = -2

Query: 559 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKI-EGHEQQCKRLVLQYIPLILVNGEK 383
           C  C  L+  +   L+    + EI+  L K C  + + +++QC   V +Y PL+L    +
Sbjct: 442 CEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVE 501

Query: 382 FLEKNDICTIVQACDAGKKTVLGS 311
            ++   +C+ +  C +  K +LG+
Sbjct: 502 VMDPGFVCSKIGVCPSAYKLLLGT 525



 Score = 36.6 bits (83), Expect = 0.068
 Identities = 23/79 (29%), Positives = 38/79 (48%), Gaps = 4/79 (5%)
 Frame = -2

Query: 559 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKIEGH--EQQCKRLVLQYIPLIL--VN 392
           C  C  ++ E  + LKD   + EI+  L K C+ I        CK +V  Y+P+IL  + 
Sbjct: 63  CDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIK 122

Query: 391 GEKFLEKNDICTIVQACDA 335
           GE      ++C+ +  C +
Sbjct: 123 GE-MSNPGEVCSALNLCQS 140



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>OXAA_DEIRA (Q9RSH5) Membrane protein oxaA|
          Length = 428

 Score = 33.9 bits (76), Expect = 0.44
 Identities = 16/40 (40%), Positives = 24/40 (60%)
 Frame = +2

Query: 185 ASRILDTYVQQEELVHYVSSGFTRLGVVFLPSFIAQQTLL 304
           A+  L+ Y  + EL+H   SG+T L  +F P+F  Q +LL
Sbjct: 159 ATSSLEVYGGKNELIHLYQSGYTELPGLFQPNFFGQISLL 198



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>NF1_MOUSE (Q04690) Neurofibromin (Neurofibromatosis-related protein NF-1)|
          Length = 2841

 Score = 32.3 bits (72), Expect = 1.3
 Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 5/64 (7%)
 Frame = -2

Query: 544 HLMDEVLSKLKDPDAEFEII-----QILLKECKKIEGHEQQCKRLVLQYIPLILVNGEKF 380
           H +D +   L +PDA  E       Q+L   CKK+  H+      +L+++  IL+   KF
Sbjct: 553 HQLDSI--DLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKF 610

Query: 379 LEKN 368
           L KN
Sbjct: 611 LLKN 614



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>NF1_HUMAN (P21359) Neurofibromin (Neurofibromatosis-related protein NF-1)|
           [Contains: Neurofibromin truncated]
          Length = 2839

 Score = 32.3 bits (72), Expect = 1.3
 Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 5/64 (7%)
 Frame = -2

Query: 544 HLMDEVLSKLKDPDAEFEII-----QILLKECKKIEGHEQQCKRLVLQYIPLILVNGEKF 380
           H +D +   L +PDA  E       Q+L   CKK+  H+      +L+++  IL+   KF
Sbjct: 553 HQLDSI--DLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKF 610

Query: 379 LEKN 368
           L KN
Sbjct: 611 LLKN 614



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>NF1_RAT (P97526) Neurofibromin (Neurofibromatosis-related protein NF-1)|
          Length = 2820

 Score = 32.3 bits (72), Expect = 1.3
 Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 5/64 (7%)
 Frame = -2

Query: 544 HLMDEVLSKLKDPDAEFEII-----QILLKECKKIEGHEQQCKRLVLQYIPLILVNGEKF 380
           H +D +   L +PDA  E       Q+L   CKK+  H+      +L+++  IL+   KF
Sbjct: 553 HQLDSI--DLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKF 610

Query: 379 LEKN 368
           L KN
Sbjct: 611 LLKN 614



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>SYP32_ARATH (Q9LK09) Syntaxin-32 (AtSYP32)|
          Length = 347

 Score = 29.6 bits (65), Expect = 8.3
 Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
 Frame = +2

Query: 140 IIQSIQRMQQQHAAKASRILDTYVQ-QEELVHYVSSGFTRLGVVF--LPSFIAQQ 295
           ++Q  Q+ QQQ   +   + DTY+Q + E +H V S    L  +F  L + ++QQ
Sbjct: 235 LLQQSQQQQQQQQQQMVPLQDTYMQGRAEALHTVESTIHELSSIFTQLATMVSQQ 289



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>SEN54_SCHPO (O74908) Probable tRNA-splicing endonuclease subunit sen54|
           (tRNA-intron endonuclease sen54)
          Length = 384

 Score = 29.6 bits (65), Expect = 8.3
 Identities = 11/32 (34%), Positives = 19/32 (59%)
 Frame = -1

Query: 383 VPREERYMHHRSSMRCWQKDGARVILRGGFAA 288
           +P E  Y+  R SM CW ++G  + L+  ++A
Sbjct: 120 LPEETLYLVERGSMECWSEEGLPMSLQAVYSA 151



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>YGEZ_ECOLI (Q46806) Hypothetical hydrolase ygeZ (EC 3.-.-.-)|
          Length = 461

 Score = 29.6 bits (65), Expect = 8.3
 Identities = 23/78 (29%), Positives = 31/78 (39%)
 Frame = +1

Query: 1   LLARQTETRFSIYSLFRYHYKLNTDQVISTTRLQVDIRNCSSYRSLYNTKHPENAAAARS 180
           ++  +  + F +Y    Y YKLN D+V+   R   +            T HPEN AA  S
Sbjct: 141 MIVEEGLSSFKLY--LTYQYKLNDDEVLQALRRLHE-------SGALTTVHPENDAAIAS 191

Query: 181 KSQSNFGHLCTTRRTSAL 234
           K         T  R  AL
Sbjct: 192 KRAEFIAAGLTAPRYHAL 209



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>GLRX1_YEAST (P25373) Glutaredoxin-1 (Glutathione-dependent oxidoreductase 1)|
          Length = 110

 Score = 29.6 bits (65), Expect = 8.3
 Identities = 21/75 (28%), Positives = 40/75 (53%), Gaps = 3/75 (4%)
 Frame = -2

Query: 577 VRSESTCVFCHHLMDEVLSKLKDPDAEFEIIQIL-LKECKKIEG--HEQQCKRLVLQYIP 407
           V S++ C +CH  ++ +  KLK P ++  ++Q+  +KE   I+   +E   +R V    P
Sbjct: 21  VASKTYCPYCHAALNTLFEKLKVPRSKVLVLQLNDMKEGADIQAALYEINGQRTV----P 76

Query: 406 LILVNGEKFLEKNDI 362
            I +NG+     +D+
Sbjct: 77  NIYINGKHIGGNDDL 91


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 93,578,660
Number of Sequences: 219361
Number of extensions: 1910053
Number of successful extensions: 5436
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 5258
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5432
length of database: 80,573,946
effective HSP length: 108
effective length of database: 56,882,958
effective search space used: 6314008338
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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