ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd14f04
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1NOS_ORYSA (Q6YPG5) Putative nitric-oxide synthase (EC 1.14.13.39) 168 7e-42
2NOS1_ARATH (Q66GP9) Nitric-oxide synthase 1 (EC 1.14.13.39) (AtN... 107 2e-23
3METH_MYCLE (Q49775) Methionine synthase (EC 2.1.1.13) (5-methylt... 29 8.9

>NOS_ORYSA (Q6YPG5) Putative nitric-oxide synthase (EC 1.14.13.39)|
          Length = 547

 Score =  168 bits (426), Expect = 7e-42
 Identities = 82/111 (73%), Positives = 86/111 (77%)
 Frame = -3

Query: 517 GQXRAEXWCGLQGVRELQIKYEELDRPASDIAISGLGWIAVEPLGVXXXXXXXXXXXXXX 338
           G+ +AE W GLQGVRELQIKYEE DRPA DIAISGLGW+AVEPLGV              
Sbjct: 437 GKEKAEGWVGLQGVRELQIKYEESDRPACDIAISGLGWVAVEPLGVPSSNPDESAEEEDN 496

Query: 337 XXXELHLRVHVPKPVXVFVRAPLPVGKAASQWYRYQELTEVEEELRPKWHY 185
              ELHLRVHVPKPV +FVR PLPVGKAASQWYRYQELTE EEELRPKWHY
Sbjct: 497 ESGELHLRVHVPKPVEIFVRPPLPVGKAASQWYRYQELTEEEEELRPKWHY 547



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>NOS1_ARATH (Q66GP9) Nitric-oxide synthase 1 (EC 1.14.13.39) (AtNOS1)|
          Length = 561

 Score =  107 bits (268), Expect = 2e-23
 Identities = 51/111 (45%), Positives = 73/111 (65%)
 Frame = -3

Query: 517 GQXRAEXWCGLQGVRELQIKYEELDRPASDIAISGLGWIAVEPLGVXXXXXXXXXXXXXX 338
           G+ + + W GLQ  R LQI+  +  RPASD+AISGLGWI++EP+                
Sbjct: 454 GKNQMQEWKGLQSHRLLQIEINDAKRPASDVAISGLGWISIEPI---RKTRGTEPRDLNE 510

Query: 337 XXXELHLRVHVPKPVXVFVRAPLPVGKAASQWYRYQELTEVEEELRPKWHY 185
              E+H+ V VPKPV VF+R  LP+G + ++WY+Y+ELT+ EEE+RPKW++
Sbjct: 511 AEHEIHICVSVPKPVEVFLRPTLPIGTSGTEWYQYRELTDKEEEVRPKWYF 561



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>METH_MYCLE (Q49775) Methionine synthase (EC 2.1.1.13)|
           (5-methyltetrahydrofolate--homocysteine
           methyltransferase) (Methionine synthase, vitamin-B12
           dependent isozyme) (MS)
          Length = 1206

 Score = 28.9 bits (63), Expect = 8.9
 Identities = 24/68 (35%), Positives = 24/68 (35%), Gaps = 2/68 (2%)
 Frame = -3

Query: 415 GLGWIAVEPLGVXXXXXXXXXXXXXXXXXELHLRVHVPKPVXVFVRAPLPVGKAASQWYR 236
           G    AVEPLGV                   HL  H   PV V   A LPV  A    Y 
Sbjct: 215 GAALAAVEPLGVDMIGLNCATGPAEMSEHLRHLSKHARIPVSVMPNAGLPVLGAKGAEYP 274

Query: 235 YQ--ELTE 218
            Q  EL E
Sbjct: 275 LQPDELAE 282


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,049,163
Number of Sequences: 219361
Number of extensions: 1126538
Number of successful extensions: 2766
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 2715
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2765
length of database: 80,573,946
effective HSP length: 104
effective length of database: 57,760,402
effective search space used: 3927707336
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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