ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd14f03
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1NOS_ORYSA (Q6YPG5) Putative nitric-oxide synthase (EC 1.14.13.39) 181 1e-57
2NOS1_ARATH (Q66GP9) Nitric-oxide synthase 1 (EC 1.14.13.39) (AtN... 115 5e-32
3CBP_MOUSE (P45481) CREB-binding protein (EC 2.3.1.48) 30 4.2
4CUBN_HUMAN (O60494) Cubilin precursor (Intrinsic factor-cobalami... 30 5.6
5CBP_HUMAN (Q92793) CREB-binding protein (EC 2.3.1.48) 30 7.2
6ADA15_MOUSE (O88839) ADAM 15 precursor (EC 3.4.24.-) (A disinteg... 29 9.5

>NOS_ORYSA (Q6YPG5) Putative nitric-oxide synthase (EC 1.14.13.39)|
          Length = 547

 Score =  181 bits (459), Expect(2) = 1e-57
 Identities = 87/114 (76%), Positives = 91/114 (79%)
 Frame = -2

Query: 528 PPTGQERAEGWCGLQGVRELQIKYEELDRPASDIAISGLGWIAVEPLGVXXXXXXXXXXX 349
           PP G+E+AEGW GLQGVRELQIKYEE DRPA DIAISGLGW+AVEPLGV           
Sbjct: 434 PPAGKEKAEGWVGLQGVRELQIKYEESDRPACDIAISGLGWVAVEPLGVPSSNPDESAEE 493

Query: 348 XXXXXXELHLRVHVPKPVEVFVRAPLPVGKAASQWYRYQELTEVEEELRPKWHY 187
                 ELHLRVHVPKPVE+FVR PLPVGKAASQWYRYQELTE EEELRPKWHY
Sbjct: 494 EDNESGELHLRVHVPKPVEIFVRPPLPVGKAASQWYRYQELTEEEEELRPKWHY 547



 Score = 62.0 bits (149), Expect(2) = 1e-57
 Identities = 28/30 (93%), Positives = 29/30 (96%)
 Frame = -1

Query: 613 GPKKLSINMVPTTEADEFYKREVGVTLTPP 524
           GPKKL INMVPTTEADEFY+REVGVTLTPP
Sbjct: 406 GPKKLKINMVPTTEADEFYEREVGVTLTPP 435



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>NOS1_ARATH (Q66GP9) Nitric-oxide synthase 1 (EC 1.14.13.39) (AtNOS1)|
          Length = 561

 Score =  115 bits (289), Expect(2) = 5e-32
 Identities = 54/114 (47%), Positives = 77/114 (67%)
 Frame = -2

Query: 528 PPTGQERAEGWCGLQGVRELQIKYEELDRPASDIAISGLGWIAVEPLGVXXXXXXXXXXX 349
           PP+G+ + + W GLQ  R LQI+  +  RPASD+AISGLGWI++EP+             
Sbjct: 451 PPSGKNQMQEWKGLQSHRLLQIEINDAKRPASDVAISGLGWISIEPI---RKTRGTEPRD 507

Query: 348 XXXXXXELHLRVHVPKPVEVFVRAPLPVGKAASQWYRYQELTEVEEELRPKWHY 187
                 E+H+ V VPKPVEVF+R  LP+G + ++WY+Y+ELT+ EEE+RPKW++
Sbjct: 508 LNEAEHEIHICVSVPKPVEVFLRPTLPIGTSGTEWYQYRELTDKEEEVRPKWYF 561



 Score = 41.6 bits (96), Expect(2) = 5e-32
 Identities = 18/31 (58%), Positives = 23/31 (74%)
 Frame = -1

Query: 613 GPKKLSINMVPTTEADEFYKREVGVTLTPPN 521
           GPK L I+ VPT  A  FY++E+GV LTPP+
Sbjct: 423 GPKALEIHAVPTKTATAFYEKELGVLLTPPS 453



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>CBP_MOUSE (P45481) CREB-binding protein (EC 2.3.1.48)|
          Length = 2441

 Score = 30.4 bits (67), Expect = 4.2
 Identities = 16/50 (32%), Positives = 23/50 (46%)
 Frame = +2

Query: 386 PSGSTAIHPSPDIAMSLAGLSSSSYFICNSRTP*SPHHPSALSWPVGGSQ 535
           P   + +HP+P  A + AG+ S  +      TP  P  P+  S PV   Q
Sbjct: 849 PVTQSPLHPTPPPASTAAGMPSLQHPTAPGMTPPQPAAPTQPSTPVSSGQ 898



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>CUBN_HUMAN (O60494) Cubilin precursor (Intrinsic factor-cobalamin receptor)|
            (Intrinsic factor-vitamin B12 receptor) (460 kDa
            receptor) (Intestinal intrinsic factor receptor)
          Length = 3623

 Score = 30.0 bits (66), Expect = 5.6
 Identities = 15/44 (34%), Positives = 25/44 (56%), Gaps = 4/44 (9%)
 Frame = +1

Query: 4    RGFLNRFHQL----ILS*NYEFISLSDHPRNIMSGRNCNWITQS 123
            RGF  +F Q     IL+ +++ +S    P N  + +NC+WI Q+
Sbjct: 1610 RGFRAQFRQACGGHILTSSFDTVSSPRFPANYPNNQNCSWIIQA 1653



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>CBP_HUMAN (Q92793) CREB-binding protein (EC 2.3.1.48)|
          Length = 2442

 Score = 29.6 bits (65), Expect = 7.2
 Identities = 16/49 (32%), Positives = 23/49 (46%)
 Frame = +2

Query: 386 PSGSTAIHPSPDIAMSLAGLSSSSYFICNSRTP*SPHHPSALSWPVGGS 532
           P   + +HP+P  A + AG+ S  +      TP  P  P+  S PV  S
Sbjct: 847 PVTQSPLHPTPPPASTAAGMPSLQHTTPPGMTPPQPAAPTQPSTPVSSS 895



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>ADA15_MOUSE (O88839) ADAM 15 precursor (EC 3.4.24.-) (A disintegrin and|
           metalloproteinase domain 15) (Metalloproteinase-like,
           disintegrin-like, and cysteine-rich protein 15) (MDC-15)
           (Metalloprotease RGD disintegrin protein) (Metargidin)
           (AD56)
          Length = 864

 Score = 29.3 bits (64), Expect = 9.5
 Identities = 28/81 (34%), Positives = 34/81 (41%), Gaps = 2/81 (2%)
 Frame = +3

Query: 288 RPRLVWVHVLLNATRHCRR--LLPQCCYQGR*MAHQVVQPRSIRVQISQCH*QAYQALHI 461
           RP LVW     + TR       L  CCY+GR   H    P S    +S C     + L +
Sbjct: 95  RPTLVWYQP--DGTRMVSEGYSLENCCYRGRVQGH----PSS---WVSLCACSGIRGLIV 145

Query: 462 LSAIRERPEAHTILQLSPGRL 524
           LS     PE    L+L PG L
Sbjct: 146 LS-----PERGYTLELGPGDL 161


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,933,874
Number of Sequences: 219361
Number of extensions: 1653697
Number of successful extensions: 4101
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 3970
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4095
length of database: 80,573,946
effective HSP length: 107
effective length of database: 57,102,319
effective search space used: 5481822624
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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