ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd13o15
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1ICIW_WHEAT (P82977) Subtilisin-chymotrypsin inhibitor WSCI 52 4e-08
2ICI3_HORVU (P08626) Subtilisin-chymotrypsin inhibitor-2B (CI-2B)... 55 1e-07
3ICI2_HORVU (P01053) Subtilisin-chymotrypsin inhibitor-2A (CI-2A) 55 1e-07
4ICIB_HORVU (P16063) Subtilisin-chymotrypsin inhibitor CI-1B 48 1e-05
5ICIC_HORVU (P01054) Subtilisin-chymotrypsin inhibitor CI-1C 47 3e-05
6ICIA_HORVU (P16062) Subtilisin-chymotrypsin inhibitor CI-1A 46 6e-05
7ICI_LUMTE (P83472) Chymotrypsin inhibitor precursor (LTCI) 45 1e-04
8ICI1_CANLI (P81712) Subtilisin inhibitor CLSI-I 44 2e-04
9ICI1_PHAAN (P16064) Subtilisin inhibitor 1 (Subtilisin inhibitor... 43 5e-04
10ICIS_VICFA (P08820) Subtilisin inhibitor (Fragment) 42 7e-04
11ITH5_CUCMA (P19873) Inhibitor of trypsin and hageman factor (CMT... 35 0.003
12BGIA_MOMCH (P24076) Glu S.griseus protease inhibitor (BGIA) 39 0.006
13ICI_EISHO (P83830) Chymotrypsin inhibitor (EHCI) (Fragment) 35 0.080
14ICI1_LYCES (P05118) Wound-induced proteinase inhibitor 1 precurs... 34 0.18
15ITR1_NICSY (Q02214) Trypsin inhibitor 1 precursor (Trypsin inhib... 32 1.2
16Y541_CHLPN (Q9Z811) Putative periplasmic metal-binding protein C... 31 1.5
17ICIA_SOLTU (P01052) Chymotrypsin inhibitor I, A, B and C subunits 31 2.0
18ICI_LINUS (P82381) Proteinase inhibitor (LUTI) 30 2.6
19RF1_MYCGA (Q7NBX3) Peptide chain release factor 1 (RF-1) 30 3.3
20LAMA5_MOUSE (Q61001) Laminin alpha-5 chain precursor 30 4.4
21YJ85_YEAST (P47152) Hypothetical 19.0 kDa protein in NNF1-STE24 ... 30 4.4
22CHS2_PHAEX (P30591) Chitin synthase 2 (EC 2.4.1.16) (Chitin-UDP ... 30 4.4
23VG42_HAEIN (P44236) Mu-like prophage FluMu protein gp42 29 5.7
24HEMH_HELHP (Q7VHH1) Ferrochelatase (EC 4.99.1.1) (Protoheme ferr... 29 7.5
25COLQ_RAT (O35167) Acetylcholinesterase collagenic tail peptide p... 29 7.5
26PHLA1_MOUSE (Q62392) Pleckstrin homology-like domain family A me... 29 7.5
27NEP1_MOUSE (O35130) Probable ribosome biogenesis protein NEP1 (N... 28 9.7
28PYRC_AQUAE (O66990) Dihydroorotase (EC 3.5.2.3) (DHOase) 28 9.7
29SO1C1_MACFA (Q9GMU6) Solute carrier organic anion transporter fa... 28 9.7

>ICIW_WHEAT (P82977) Subtilisin-chymotrypsin inhibitor WSCI|
          Length = 72

 Score = 52.4 bits (124), Expect(2) = 4e-08
 Identities = 22/40 (55%), Positives = 33/40 (82%)
 Frame = -3

Query: 388 KTEWPELVGCTIKEAKEKIKADRPDLKVVIVPVGSIVTQE 269
           KTEWPELVG +++EAK+ I  D+ + ++V++PVG+IVT E
Sbjct: 10  KTEWPELVGKSVEEAKKVILQDKSEAQIVVLPVGTIVTME 49



 Score = 23.9 bits (50), Expect(2) = 4e-08
 Identities = 10/21 (47%), Positives = 18/21 (85%), Gaps = 3/21 (14%)
 Frame = -1

Query: 261 LNRVRVWVD---KVAKVPKIG 208
           ++RVR++VD   K+A+VP++G
Sbjct: 52  IDRVRLFVDSLDKIAQVPRVG 72



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>ICI3_HORVU (P08626) Subtilisin-chymotrypsin inhibitor-2B (CI-2B) (Fragment)|
          Length = 72

 Score = 55.1 bits (131), Expect = 1e-07
 Identities = 22/44 (50%), Positives = 34/44 (77%)
 Frame = -3

Query: 400 CDGAKTEWPELVGCTIKEAKEKIKADRPDLKVVIVPVGSIVTQE 269
           C   KTEWPELV  +++EAK+ I  D+P+ +++++PVG+IVT E
Sbjct: 6   CQNQKTEWPELVEKSVEEAKKVILQDKPEAQIIVLPVGTIVTME 49



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>ICI2_HORVU (P01053) Subtilisin-chymotrypsin inhibitor-2A (CI-2A)|
          Length = 83

 Score = 55.1 bits (131), Expect = 1e-07
 Identities = 22/40 (55%), Positives = 34/40 (85%)
 Frame = -3

Query: 388 KTEWPELVGCTIKEAKEKIKADRPDLKVVIVPVGSIVTQE 269
           KTEWPELVG +++EAK+ I  D+P+ +++++PVG+IVT E
Sbjct: 21  KTEWPELVGKSVEEAKKVILQDKPEAQIIVLPVGTIVTME 60



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>ICIB_HORVU (P16063) Subtilisin-chymotrypsin inhibitor CI-1B|
          Length = 83

 Score = 48.1 bits (113), Expect = 1e-05
 Identities = 22/52 (42%), Positives = 34/52 (65%)
 Frame = -3

Query: 409 SKSCDGAKTEWPELVGCTIKEAKEKIKADRPDLKVVIVPVGSIVTQEIDFEP 254
           S    GAK  WPE+VG + ++AKE I  D+PD ++ ++PV ++V   +DF P
Sbjct: 18  SIGASGAKRSWPEVVGMSAEKAKEIILRDKPDAQIEVIPVDAMV--PLDFNP 67



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>ICIC_HORVU (P01054) Subtilisin-chymotrypsin inhibitor CI-1C|
          Length = 77

 Score = 46.6 bits (109), Expect = 3e-05
 Identities = 21/52 (40%), Positives = 35/52 (67%)
 Frame = -3

Query: 409 SKSCDGAKTEWPELVGCTIKEAKEKIKADRPDLKVVIVPVGSIVTQEIDFEP 254
           S    GAKT WPE+VG + ++AKE I  D+P+ ++ ++PV ++V   ++F P
Sbjct: 8   SIGASGAKTSWPEVVGMSAEKAKEIILRDKPNAQIEVIPVDAMV--PLNFNP 57



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>ICIA_HORVU (P16062) Subtilisin-chymotrypsin inhibitor CI-1A|
          Length = 83

 Score = 45.8 bits (107), Expect = 6e-05
 Identities = 21/52 (40%), Positives = 35/52 (67%)
 Frame = -3

Query: 409 SKSCDGAKTEWPELVGCTIKEAKEKIKADRPDLKVVIVPVGSIVTQEIDFEP 254
           S     AKT WPE+VG + ++AKE I  D+P+ +V ++PV ++V   ++F+P
Sbjct: 18  SIGASSAKTSWPEVVGMSAEKAKEIILRDKPNAQVEVIPVDAMV--HLNFDP 67



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>ICI_LUMTE (P83472) Chymotrypsin inhibitor precursor (LTCI)|
          Length = 86

 Score = 44.7 bits (104), Expect = 1e-04
 Identities = 23/45 (51%), Positives = 29/45 (64%)
 Frame = -3

Query: 388 KTEWPELVGCTIKEAKEKIKADRPDLKVVIVPVGSIVTQEIDFEP 254
           KT WPELVG T++EAK +I  DRPD  + + P  S VT   D+ P
Sbjct: 24  KTSWPELVGETLEEAKAQILEDRPDAVIKVQPEHSPVT--YDYRP 66



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>ICI1_CANLI (P81712) Subtilisin inhibitor CLSI-I|
          Length = 65

 Score = 44.3 bits (103), Expect = 2e-04
 Identities = 23/46 (50%), Positives = 32/46 (69%)
 Frame = -3

Query: 388 KTEWPELVGCTIKEAKEKIKADRPDLKVVIVPVGSIVTQEIDFEPR 251
           KT WPELVG T +EA EKIK +   +++ +VP GS VT   D++P+
Sbjct: 4   KTSWPELVGVTAEEA-EKIKEEMSGVEIQVVPPGSFVT--ADYKPQ 46



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>ICI1_PHAAN (P16064) Subtilisin inhibitor 1 (Subtilisin inhibitor I) (ASI-I)|
           [Contains: Subtilisin inhibitor 2 (Subtilisin inhibitor
           II) (ASI-II)]
          Length = 92

 Score = 42.7 bits (99), Expect = 5e-04
 Identities = 20/46 (43%), Positives = 30/46 (65%)
 Frame = -3

Query: 388 KTEWPELVGCTIKEAKEKIKADRPDLKVVIVPVGSIVTQEIDFEPR 251
           KT WPELVG T ++A+ KIK +  D+++ + P  S VT   D+ P+
Sbjct: 30  KTSWPELVGVTAEQAETKIKEEMVDVQIQVSPHDSFVT--ADYNPK 73



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>ICIS_VICFA (P08820) Subtilisin inhibitor (Fragment)|
          Length = 62

 Score = 42.4 bits (98), Expect = 7e-04
 Identities = 19/44 (43%), Positives = 30/44 (68%)
 Frame = -3

Query: 388 KTEWPELVGCTIKEAKEKIKADRPDLKVVIVPVGSIVTQEIDFE 257
           +T WPELVG + +EA+ KIK + P+ ++ +VP  S VT +  F+
Sbjct: 1   RTSWPELVGVSAEEAR-KIKEEMPEAEIQVVPQDSFVTADYKFQ 43



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>ITH5_CUCMA (P19873) Inhibitor of trypsin and hageman factor (CMTI-V)|
          Length = 68

 Score = 35.4 bits (80), Expect(2) = 0.003
 Identities = 19/45 (42%), Positives = 27/45 (60%)
 Frame = -3

Query: 403 SCDGAKTEWPELVGCTIKEAKEKIKADRPDLKVVIVPVGSIVTQE 269
           SC G K+ WP LVG     AK  I+   P++K VI+  G+ VT++
Sbjct: 2   SCPG-KSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKD 45



 Score = 24.3 bits (51), Expect(2) = 0.003
 Identities = 11/20 (55%), Positives = 14/20 (70%), Gaps = 3/20 (15%)
 Frame = -1

Query: 258 NRVRVWVDK---VAKVPKIG 208
           NRVR+WV+K   V   P+IG
Sbjct: 49  NRVRIWVNKRGLVVSPPRIG 68



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>BGIA_MOMCH (P24076) Glu S.griseus protease inhibitor (BGIA)|
          Length = 68

 Score = 39.3 bits (90), Expect = 0.006
 Identities = 21/44 (47%), Positives = 27/44 (61%)
 Frame = -3

Query: 400 CDGAKTEWPELVGCTIKEAKEKIKADRPDLKVVIVPVGSIVTQE 269
           C G K  WP+LVG T   AK  I+ + P ++ VIV VGS VT +
Sbjct: 3   CQG-KRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTAD 45



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>ICI_EISHO (P83830) Chymotrypsin inhibitor (EHCI) (Fragment)|
          Length = 41

 Score = 35.4 bits (80), Expect = 0.080
 Identities = 15/31 (48%), Positives = 22/31 (70%)
 Frame = -3

Query: 388 KTEWPELVGCTIKEAKEKIKADRPDLKVVIV 296
           K  WPELVG T +EA+ +I  D+P+  VV++
Sbjct: 7   KAAWPELVGKTPEEARAQILLDKPNANVVVL 37



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>ICI1_LYCES (P05118) Wound-induced proteinase inhibitor 1 precursor|
           (Wound-induced proteinase inhibitor I)
          Length = 111

 Score = 34.3 bits (77), Expect = 0.18
 Identities = 22/54 (40%), Positives = 31/54 (57%), Gaps = 1/54 (1%)
 Frame = -3

Query: 433 LSHFVSNMSKSCDGAKTEWPELVGCTIKEAKEKIKADRPDL-KVVIVPVGSIVT 275
           L  F SN+   C+G K  WPEL+G   K AKE I+ + P +  + I+  GS +T
Sbjct: 36  LKEFDSNLM--CEG-KQMWPELIGVPTKLAKEIIEKENPSITNIPILLSGSPIT 86



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>ITR1_NICSY (Q02214) Trypsin inhibitor 1 precursor (Trypsin inhibitor I)|
           (NSTI-I)
          Length = 94

 Score = 31.6 bits (70), Expect = 1.2
 Identities = 22/51 (43%), Positives = 28/51 (54%), Gaps = 2/51 (3%)
 Frame = -3

Query: 403 SCDGAKTE-WPELVGCTIKEAKEKIKADRPDL-KVVIVPVGSIVTQEIDFE 257
           SC G K E WPEL+G   K A+E I+ +   L  V  V  GS VT++   E
Sbjct: 25  SCPGNKKETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCE 75



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>Y541_CHLPN (Q9Z811) Putative periplasmic metal-binding protein|
           CPn_0541/CP0211/CPj0541/CpB0562 precursor
          Length = 278

 Score = 31.2 bits (69), Expect = 1.5
 Identities = 16/51 (31%), Positives = 26/51 (50%), Gaps = 2/51 (3%)
 Frame = +1

Query: 25  HYITHPYFPAKN--THTHTHIHTFSAKHPQHSCYVSPKGTLLIYSVD*LDD 171
           +Y TH +   KN      T + T S K+PQH+      G  L+ ++D L++
Sbjct: 119 NYDTHTWLSPKNLKVQVETIVTTLSKKYPQHATLYQSNGEKLLLALDQLNE 169



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>ICIA_SOLTU (P01052) Chymotrypsin inhibitor I, A, B and C subunits|
          Length = 71

 Score = 30.8 bits (68), Expect = 2.0
 Identities = 20/45 (44%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
 Frame = -3

Query: 400 CDGAKTEWPELVGCTIKEAKEKIKADRPDL-KVVIVPVGSIVTQE 269
           CDG K +WPEL+G   K AKE I+     +  V I+  GS VT +
Sbjct: 5   CDG-KLQWPELIGVPTKLAKEIIEKQNSLISNVHILLNGSPVTMD 48



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>ICI_LINUS (P82381) Proteinase inhibitor (LUTI)|
          Length = 69

 Score = 30.4 bits (67), Expect = 2.6
 Identities = 14/47 (29%), Positives = 27/47 (57%)
 Frame = -3

Query: 409 SKSCDGAKTEWPELVGCTIKEAKEKIKADRPDLKVVIVPVGSIVTQE 269
           S+ C G K  WPELVG +   A   ++ +  ++  +++  GS +T++
Sbjct: 1   SRRCPG-KNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKD 46



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>RF1_MYCGA (Q7NBX3) Peptide chain release factor 1 (RF-1)|
          Length = 360

 Score = 30.0 bits (66), Expect = 3.3
 Identities = 21/60 (35%), Positives = 29/60 (48%)
 Frame = -3

Query: 397 DGAKTEWPELVGCTIKEAKEKIKADRPDLKVVIVPVGSIVTQEIDFEPRPCLG*QGGEGS 218
           D A  E+ EL    +  AKE+I      LKV+++P      +E+  E RP  G  G E S
Sbjct: 71  DNAMKEFHELAILDLDAAKEQIPDLEEKLKVLLLPADPNDDKEVIVEMRPAAG--GDESS 128



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>LAMA5_MOUSE (Q61001) Laminin alpha-5 chain precursor|
          Length = 3718

 Score = 29.6 bits (65), Expect = 4.4
 Identities = 19/45 (42%), Positives = 20/45 (44%), Gaps = 1/45 (2%)
 Frame = -2

Query: 425  FRFQHEQVMRRCQDGVA*AG-RVHDQGGQRKDQGGPAGPQSRHCA 294
            F F+  Q  R C  G A  G   H Q GQ   Q G  GPQ   CA
Sbjct: 2061 FGFEQCQGCRPCACGPAAKGSECHPQSGQCHCQPGTTGPQCLECA 2105



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>YJ85_YEAST (P47152) Hypothetical 19.0 kDa protein in NNF1-STE24 intergenic|
           region
          Length = 169

 Score = 29.6 bits (65), Expect = 4.4
 Identities = 14/29 (48%), Positives = 17/29 (58%)
 Frame = +1

Query: 58  NTHTHTHIHTFSAKHPQHSCYVSPKGTLL 144
           NTHTHTH HT +  +      VS +G LL
Sbjct: 17  NTHTHTHTHTHTHIYIYTGDQVSVRGRLL 45



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>CHS2_PHAEX (P30591) Chitin synthase 2 (EC 2.4.1.16) (Chitin-UDP|
           acetyl-glucosaminyl transferase 2) (Class-II chitin
           synthase 2) (Fragment)
          Length = 189

 Score = 29.6 bits (65), Expect = 4.4
 Identities = 13/34 (38%), Positives = 21/34 (61%)
 Frame = -3

Query: 433 LSHFVSNMSKSCDGAKTEWPELVGCTIKEAKEKI 332
           +SHF S  +KS    K  WP++V C I + ++K+
Sbjct: 15  ISHFCSR-TKSRTWGKDGWPKIVVCVIADGRQKV 47



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>VG42_HAEIN (P44236) Mu-like prophage FluMu protein gp42|
          Length = 631

 Score = 29.3 bits (64), Expect = 5.7
 Identities = 13/41 (31%), Positives = 25/41 (60%)
 Frame = -3

Query: 397 DGAKTEWPELVGCTIKEAKEKIKADRPDLKVVIVPVGSIVT 275
           DG   ++P+ V  T+ EA + +K    D+K V+  +GS+++
Sbjct: 286 DGTLDDFPKTVSDTLIEALKNLKEMAKDVKPVLESIGSVMS 326



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>HEMH_HELHP (Q7VHH1) Ferrochelatase (EC 4.99.1.1) (Protoheme ferro-lyase) (Heme|
           synthetase)
          Length = 311

 Score = 28.9 bits (63), Expect = 7.5
 Identities = 12/38 (31%), Positives = 21/38 (55%)
 Frame = -3

Query: 376 PELVGCTIKEAKEKIKADRPDLKVVIVPVGSIVTQEID 263
           P+ + C I+  KE + AD P+  V+++   S+    ID
Sbjct: 150 PDYISCIIERIKESLGADNPNEFVLLLSAHSLPQSRID 187



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>COLQ_RAT (O35167) Acetylcholinesterase collagenic tail peptide precursor|
           (AChE Q subunit) (Acetylcholinesterase-associated
           collagen)
          Length = 458

 Score = 28.9 bits (63), Expect = 7.5
 Identities = 19/62 (30%), Positives = 22/62 (35%)
 Frame = -2

Query: 449 IELVAFVPFRFQHEQVMRRCQDGVA*AGRVHDQGGQRKDQGGPAGPQSRHCAGRKYCDTG 270
           I+L  F P    H+     C DGV        Q G+  D G P          R YC  G
Sbjct: 359 IQLTPFYPVGLHHKAAWHLCGDGVL-------QPGEECDDGNPDVSDGCIDCHRAYCGDG 411

Query: 269 DR 264
            R
Sbjct: 412 YR 413



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>PHLA1_MOUSE (Q62392) Pleckstrin homology-like domain family A member 1 (T-cell|
           death-associated gene 51 protein) (Proline- and
           glutamine-rich protein) (PQR protein)
          Length = 261

 Score = 28.9 bits (63), Expect = 7.5
 Identities = 13/46 (28%), Positives = 21/46 (45%), Gaps = 3/46 (6%)
 Frame = +1

Query: 25  HYITHPY---FPAKNTHTHTHIHTFSAKHPQHSCYVSPKGTLLIYS 153
           H  +HP+    P  + H H H H +  +H     +  P+G  L+ S
Sbjct: 211 HPYSHPHQHPHPHPHPHPHPHPHPYQLQHAHQPLHSQPQGHRLLRS 256



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>NEP1_MOUSE (O35130) Probable ribosome biogenesis protein NEP1 (Nucleolar|
           protein EMG1 homolog) (Protein C2f)
          Length = 244

 Score = 28.5 bits (62), Expect = 9.7
 Identities = 15/46 (32%), Positives = 25/46 (54%), Gaps = 2/46 (4%)
 Frame = +3

Query: 195 FS*LSQSWEPSPPCQPRHGRGSK--SISCVTILPTGTMTTLRSGRS 326
           FS   Q WE +PP + R G GSK      + +L   ++ T++ G++
Sbjct: 15  FSVQEQDWETTPPKKLRLGAGSKCGGRRLIVVLEGASLETVKVGKT 60



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>PYRC_AQUAE (O66990) Dihydroorotase (EC 3.5.2.3) (DHOase)|
          Length = 422

 Score = 28.5 bits (62), Expect = 9.7
 Identities = 24/71 (33%), Positives = 34/71 (47%), Gaps = 2/71 (2%)
 Frame = +3

Query: 258 SKSISCVTILPTGTMTTLRSGRS-ALIFSLASL-IVHPTSSGHSVLAPSHDLLMLETKWD 431
           SKS+    +LPTGT+T  R G+  A  +SL     V  T  G    +P  D  ++    +
Sbjct: 113 SKSVGLCRVLPTGTITKGRKGKEIADFYSLKEAGCVAFTDDG----SPVMDSSVMRKALE 168

Query: 432 KRHQLYVPLCD 464
              QL VP+ D
Sbjct: 169 LASQLGVPIMD 179



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>SO1C1_MACFA (Q9GMU6) Solute carrier organic anion transporter family member 1C1|
           (Solute carrier family 21 member 14)
          Length = 602

 Score = 28.5 bits (62), Expect = 9.7
 Identities = 16/56 (28%), Positives = 28/56 (50%)
 Frame = +3

Query: 216 WEPSPPCQPRHGRGSKSISCVTILPTGTMTTLRSGRSALIFSLASLIVHPTSSGHS 383
           WEP   C      G   I+ V+  P G  T+ RSG++ + ++   + +  + SG+S
Sbjct: 377 WEPM--C------GENGITYVSACPAGCQTSNRSGKNIIFYNCTCVGIAASKSGNS 424


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 73,713,505
Number of Sequences: 219361
Number of extensions: 1552039
Number of successful extensions: 4403
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 4149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4355
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3304846491
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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