Clone Name | rbasd13k05 |
---|---|
Clone Library Name | barley_pub |
>MDAR_PEA (Q40977) Monodehydroascorbate reductase (EC 1.6.5.4) (MDAR)| (Ascorbate free radical reductase) (AFR reductase) Length = 433 Score = 199 bits (506), Expect(2) = 2e-58 Identities = 93/119 (78%), Positives = 107/119 (89%) Frame = -3 Query: 503 DHARKSAEQAVKAIKAKESGESVAEYDYLPYFYSRSFDVAWQFYGDNVGETVLFGDNDPA 324 DHARKSAEQA KAI A + G+SV EYDYLPYFYSRSFD++WQFYGDNVGETVLFGDNDPA Sbjct: 315 DHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPA 374 Query: 323 AAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKEGLDFATKI 147 ++KPKFGTYW+K+GKV+G FLEGG+ DEN+AIAKVARA+P V DV LA+EGL FA+KI Sbjct: 375 SSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 433 Score = 46.2 bits (108), Expect(2) = 2e-58 Identities = 20/27 (74%), Positives = 24/27 (88%) Frame = -2 Query: 585 VAGVYAIGDVATFPMKLYDEPRRVEHV 505 V VYA+GDVATFP+KLY++ RRVEHV Sbjct: 288 VPDVYAVGDVATFPLKLYNDVRRVEHV 314
>MDAR4_ARATH (Q93WJ8) Probable monodehydroascorbate reductase, cytoplasmic| isoform 4 (EC 1.6.5.4) (MDAR 4) Length = 435 Score = 193 bits (491), Expect(2) = 3e-57 Identities = 89/119 (74%), Positives = 103/119 (86%) Frame = -3 Query: 503 DHARKSAEQAVKAIKAKESGESVAEYDYLPYFYSRSFDVAWQFYGDNVGETVLFGDNDPA 324 DHARKSAEQAVKAIKA E G S+ EYDYLPYFYSR+FD++WQFYGDNVGE+VLFGDNDP Sbjct: 317 DHARKSAEQAVKAIKAAEEGNSIPEYDYLPYFYSRAFDLSWQFYGDNVGESVLFGDNDPE 376 Query: 323 AAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKEGLDFATKI 147 + KPKFG+YW+K+ KV+G FLEGGS +EN AIAK+ARAQP V +E L+KEGL FAT I Sbjct: 377 SPKPKFGSYWIKERKVVGAFLEGGSPEENNAIAKLARAQPSVESLEVLSKEGLSFATNI 435 Score = 48.1 bits (113), Expect(2) = 3e-57 Identities = 22/24 (91%), Positives = 23/24 (95%) Frame = -2 Query: 576 VYAIGDVATFPMKLYDEPRRVEHV 505 VYAIGDVATFPMKLY+E RRVEHV Sbjct: 293 VYAIGDVATFPMKLYNEMRRVEHV 316
>MDARS_CUCSA (Q42711) Monodehydroascorbate reductase, seedling isozyme (EC| 1.6.5.4) (MDAR seedling) (Ascorbate free radical reductase seedling) (AFR reductase seedling) Length = 434 Score = 182 bits (463), Expect(2) = 1e-53 Identities = 83/119 (69%), Positives = 100/119 (84%) Frame = -3 Query: 503 DHARKSAEQAVKAIKAKESGESVAEYDYLPYFYSRSFDVAWQFYGDNVGETVLFGDNDPA 324 DH+RKSAEQAVKAIKA E G+++ EYDYLPYFYSRSFD++WQFYGDNVG+ VLFGDN P Sbjct: 316 DHSRKSAEQAVKAIKASEEGKAIEEYDYLPYFYSRSFDLSWQFYGDNVGDAVLFGDNSPD 375 Query: 323 AAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKEGLDFATKI 147 +A KFG+YW+KDGKV+G FLE GS +EN+AIAKVAR QP V + L KEG+ FA+K+ Sbjct: 376 SATHKFGSYWIKDGKVVGAFLESGSPEENKAIAKVARIQPSVESSDLLLKEGISFASKV 434 Score = 47.0 bits (110), Expect(2) = 1e-53 Identities = 21/27 (77%), Positives = 24/27 (88%) Frame = -2 Query: 585 VAGVYAIGDVATFPMKLYDEPRRVEHV 505 V VYA+GDVATFP+KLY+E RRVEHV Sbjct: 289 VPDVYAVGDVATFPLKLYNELRRVEHV 315
>MDAR1_ARATH (Q9SR59) Probable monodehydroascorbate reductase, cytoplasmic| isoform 1 (EC 1.6.5.4) (MDAR 1) Length = 441 Score = 184 bits (467), Expect(2) = 8e-53 Identities = 84/119 (70%), Positives = 105/119 (88%) Frame = -3 Query: 506 SDHARKSAEQAVKAIKAKESGESVAEYDYLPYFYSRSFDVAWQFYGDNVGETVLFGDNDP 327 +D+ARKSA QAVKAIKA E G+++ +YDYLPYFYSR F ++W+FYG+NVGE+VLFGDNDP Sbjct: 317 ADNARKSAAQAVKAIKAGEEGKTIPDYDYLPYFYSRFFKLSWEFYGENVGESVLFGDNDP 376 Query: 326 AAAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKEGLDFATK 150 + KPKFGTYWVKDGKV+GVFLEGG+ +E++AIAKVARAQP V ++ L++EGL FATK Sbjct: 377 KSPKPKFGTYWVKDGKVVGVFLEGGTQEEHKAIAKVARAQPSVESLDVLSEEGLSFATK 435 Score = 42.7 bits (99), Expect(2) = 8e-53 Identities = 19/26 (73%), Positives = 21/26 (80%) Frame = -2 Query: 585 VAGVYAIGDVATFPMKLYDEPRRVEH 508 V VYA+GDVATFPMK+Y RRVEH Sbjct: 291 VPDVYALGDVATFPMKMYGGTRRVEH 316
>MDAR_LYCES (Q43497) Monodehydroascorbate reductase (EC 1.6.5.4) (MDAR)| (Ascorbate free radical reductase) (AFR reductase) Length = 433 Score = 180 bits (457), Expect(2) = 1e-52 Identities = 85/119 (71%), Positives = 102/119 (85%) Frame = -3 Query: 503 DHARKSAEQAVKAIKAKESGESVAEYDYLPYFYSRSFDVAWQFYGDNVGETVLFGDNDPA 324 DH+RKSAEQAVKAI A E G+SV EYDYLPYFYSR+FD++WQFYGDNVGETVLFGD DP Sbjct: 316 DHSRKSAEQAVKAIFASEQGKSVDEYDYLPYFYSRAFDLSWQFYGDNVGETVLFGDADPN 375 Query: 323 AAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKEGLDFATKI 147 +A KFG YW+KDGK++G FLE GS +EN+AIAKVA+ QPP A ++ LA+EG+ FA+KI Sbjct: 376 SATHKFGQYWIKDGKIVGAFLESGSPEENKAIAKVAKVQPP-ATLDQLAQEGISFASKI 433 Score = 46.2 bits (108), Expect(2) = 1e-52 Identities = 20/27 (74%), Positives = 24/27 (88%) Frame = -2 Query: 585 VAGVYAIGDVATFPMKLYDEPRRVEHV 505 V VYA+GDVATFP+K+Y+E RRVEHV Sbjct: 289 VPDVYAVGDVATFPLKMYNEIRRVEHV 315
>MDAR3_ARATH (Q9LFA3) Probable monodehydroascorbate reductase, cytoplasmic| isoform 3 (EC 1.6.5.4) (MDAR 3) Length = 434 Score = 178 bits (452), Expect(2) = 1e-51 Identities = 79/119 (66%), Positives = 101/119 (84%) Frame = -3 Query: 503 DHARKSAEQAVKAIKAKESGESVAEYDYLPYFYSRSFDVAWQFYGDNVGETVLFGDNDPA 324 DH+RKSAEQAVKAIKA E G +V EYDYLP+FYSRSFD++WQFYGDNVG++VLFGD++P+ Sbjct: 316 DHSRKSAEQAVKAIKAAEGGAAVEEYDYLPFFYSRSFDLSWQFYGDNVGDSVLFGDSNPS 375 Query: 323 AAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKEGLDFATKI 147 KP+FG YWV+ GKV+G F+EGGS DEN+A+AKVA+A+P ++ L K+G+ FA KI Sbjct: 376 NPKPRFGAYWVQGGKVVGAFMEGGSGDENKALAKVAKARPSAESLDELVKQGISFAAKI 434 Score = 44.7 bits (104), Expect(2) = 1e-51 Identities = 19/27 (70%), Positives = 23/27 (85%) Frame = -2 Query: 585 VAGVYAIGDVATFPMKLYDEPRRVEHV 505 V VYA+GDVATFP+K+Y + RRVEHV Sbjct: 289 VPDVYAVGDVATFPLKMYGDVRRVEHV 315
>MDARF_CUCSA (P83966) Monodehydroascorbate reductase, fruit isozyme (EC 1.6.5.4)| (MDAR fruit) (Ascorbate free radical reductase fruit) (AFR reductase fruit) (Fragments) Length = 166 Score = 111 bits (278), Expect(2) = 6e-32 Identities = 57/89 (64%), Positives = 66/89 (74%) Frame = -3 Query: 440 SVAEYDYLPYFYSRSFDVAWQFYGDNVGETVLFGDNDPAAAKPKFGTYWVKDGKVIGVFL 261 S+ EYDYLPYFYSR+F++AWQFYGDNVGETVLF DN FGTYW+ KV+GVFL Sbjct: 93 SIEEYDYLPYFYSRTFNLAWQFYGDNVGETVLFPDN--------FGTYWI---KVVGVFL 141 Query: 260 EGGSADENQAIAKVARAQPPVADVEALAK 174 EGG+ DE KVAR QPPV ++ LAK Sbjct: 142 EGGTPDE----YKVARVQPPVESLDQLAK 166 Score = 45.4 bits (106), Expect(2) = 6e-32 Identities = 19/27 (70%), Positives = 24/27 (88%) Frame = -2 Query: 585 VAGVYAIGDVATFPMKLYDEPRRVEHV 505 + VYA+GDVAT+P+KLY+E RRVEHV Sbjct: 61 IPDVYAVGDVATYPLKLYNELRRVEHV 87
>MDAR2_ARATH (Q9LK94) Probable monodehydroascorbate reductase, cytoplasmic| isoform 2 (EC 1.6.5.4) (MDAR 2) Length = 488 Score = 104 bits (260), Expect(2) = 2e-29 Identities = 55/117 (47%), Positives = 73/117 (62%), Gaps = 1/117 (0%) Frame = -3 Query: 503 DHARKSAEQAVKAIKAKESGESVAEYDYLPYFYSRSFDVAWQFYGDNVGETVLFGDNDPA 324 D ARKSA AV AI ++DYLP+FYSR F +WQFYGD G+ V FG+ + Sbjct: 315 DSARKSARHAVSAIM---DPIKTGDFDYLPFFYSRVFAFSWQFYGDPTGDVVHFGEYEDG 371 Query: 323 AAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVA-DVEALAKEGLDFA 156 + FG YWVK G ++G FLEGG+ +E + I+K + +P V D+E L +EGL FA Sbjct: 372 KS---FGAYWVKKGHLVGSFLEGGTKEEYETISKATQLKPAVTIDLEELEREGLGFA 425 Score = 43.9 bits (102), Expect(2) = 2e-29 Identities = 20/34 (58%), Positives = 25/34 (73%) Frame = -2 Query: 582 AGVYAIGDVATFPMKLYDEPRRVEHVGPCSEIGR 481 + VYAIGDVATFP+KL+ E RR+EHV + R Sbjct: 289 SSVYAIGDVATFPVKLFGEMRRLEHVDSARKSAR 322
>MDARP_ARATH (P92947) Monodehydroascorbate reductase, chloroplast precursor (EC| 1.6.5.4) (MDAR) Length = 493 Score = 97.8 bits (242), Expect(2) = 7e-27 Identities = 53/126 (42%), Positives = 76/126 (60%), Gaps = 7/126 (5%) Frame = -3 Query: 503 DHARKSAEQAVKAIKAKESGESVAEYDYLPYFYSRSFD-------VAWQFYGDNVGETVL 345 DHAR+SA+ VK++ + YDYLPYFYSR F+ V WQF+GDNVGETV Sbjct: 370 DHARRSAQHCVKSLLTAHTDT----YDYLPYFYSRVFEYEGSPRKVWWQFFGDNVGETVE 425 Query: 344 FGDNDPAAAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKEGL 165 G+ DP K T+W++ G++ GV +E GS +E Q + K+AR+QP V + + + Sbjct: 426 VGNFDP-----KIATFWIESGRLKGVLVESGSPEEFQLLPKLARSQPLVDKAKLASASSV 480 Query: 164 DFATKI 147 + A +I Sbjct: 481 EEALEI 486 Score = 42.4 bits (98), Expect(2) = 7e-27 Identities = 17/25 (68%), Positives = 21/25 (84%) Frame = -2 Query: 579 GVYAIGDVATFPMKLYDEPRRVEHV 505 G++AIGDVA FP+K+YD RVEHV Sbjct: 345 GIFAIGDVAAFPLKIYDRMTRVEHV 369
>ALG14_ASPFU (Q4WNB5) UDP-N-acetylglucosamine transferase subunit alg14 (EC| 2.4.1.-) (Asparagine linked glycosylation protein 14) Length = 228 Score = 30.4 bits (67), Expect = 3.9 Identities = 15/45 (33%), Positives = 23/45 (51%) Frame = -3 Query: 341 GDNDPAAAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQ 207 GDN AA +F T W+K + G ++ E+ A+ V RA+ Sbjct: 25 GDNFSAARAVEFETEWLKQSPKLSFPANGSNSTESYAVVTVPRAR 69
>K6PP_MOUSE (Q9WUA3) 6-phosphofructokinase type C (EC 2.7.1.11)| (Phosphofructokinase 1) (Phosphohexokinase) (Phosphofructo-1-kinase isozyme C) (PFK-C) Length = 784 Score = 30.4 bits (67), Expect = 3.9 Identities = 17/43 (39%), Positives = 23/43 (53%), Gaps = 1/43 (2%) Frame = -3 Query: 338 DNDPAAAKPKFGTYWVKDGKVIGVFLEGGSAD-ENQAIAKVAR 213 D+ ++A PK+ + DGK IGV GG A N A+ V R Sbjct: 5 DSSSSSAYPKYLEHLSGDGKAIGVLTSGGDAQGMNAAVRAVVR 47
>EFTU_MYCPE (Q8EX18) Elongation factor Tu (EF-Tu)| Length = 394 Score = 29.3 bits (64), Expect = 8.7 Identities = 19/54 (35%), Positives = 25/54 (46%), Gaps = 1/54 (1%) Frame = -3 Query: 491 KSAEQAVKAIKAKESGESVAEYD-YLPYFYSRSFDVAWQFYGDNVGETVLFGDN 333 K E + A+K +E G + Y P FY R+ DV Q D E + GDN Sbjct: 303 KQFEAEIYALKKEEGGRHTPVLNGYRPQFYFRTTDVTGQITLDKGVEMINPGDN 356
>6PGL_PSEAE (Q9X2N2) 6-phosphogluconolactonase (EC 3.1.1.31) (6PGL)| Length = 238 Score = 29.3 bits (64), Expect = 8.7 Identities = 14/36 (38%), Positives = 24/36 (66%) Frame = -3 Query: 263 LEGGSADENQAIAKVARAQPPVADVEALAKEGLDFA 156 L A++ QA+ V+ + PVA +EAL++E LD++ Sbjct: 35 LRSALAEQGQALLVVSGGRSPVAFLEALSEEPLDWS 70 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 67,660,961 Number of Sequences: 219361 Number of extensions: 1152809 Number of successful extensions: 4522 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 4380 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 4514 length of database: 80,573,946 effective HSP length: 106 effective length of database: 57,321,680 effective search space used: 5044307840 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)