ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd13k05
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1MDAR_PEA (Q40977) Monodehydroascorbate reductase (EC 1.6.5.4) (M... 199 2e-58
2MDAR4_ARATH (Q93WJ8) Probable monodehydroascorbate reductase, cy... 193 3e-57
3MDARS_CUCSA (Q42711) Monodehydroascorbate reductase, seedling is... 182 1e-53
4MDAR1_ARATH (Q9SR59) Probable monodehydroascorbate reductase, cy... 184 8e-53
5MDAR_LYCES (Q43497) Monodehydroascorbate reductase (EC 1.6.5.4) ... 180 1e-52
6MDAR3_ARATH (Q9LFA3) Probable monodehydroascorbate reductase, cy... 178 1e-51
7MDARF_CUCSA (P83966) Monodehydroascorbate reductase, fruit isozy... 111 6e-32
8MDAR2_ARATH (Q9LK94) Probable monodehydroascorbate reductase, cy... 104 2e-29
9MDARP_ARATH (P92947) Monodehydroascorbate reductase, chloroplast... 98 7e-27
10ALG14_ASPFU (Q4WNB5) UDP-N-acetylglucosamine transferase subunit... 30 3.9
11K6PP_MOUSE (Q9WUA3) 6-phosphofructokinase type C (EC 2.7.1.11) (... 30 3.9
12EFTU_MYCPE (Q8EX18) Elongation factor Tu (EF-Tu) 29 8.7
136PGL_PSEAE (Q9X2N2) 6-phosphogluconolactonase (EC 3.1.1.31) (6PGL) 29 8.7

>MDAR_PEA (Q40977) Monodehydroascorbate reductase (EC 1.6.5.4) (MDAR)|
           (Ascorbate free radical reductase) (AFR reductase)
          Length = 433

 Score =  199 bits (506), Expect(2) = 2e-58
 Identities = 93/119 (78%), Positives = 107/119 (89%)
 Frame = -3

Query: 503 DHARKSAEQAVKAIKAKESGESVAEYDYLPYFYSRSFDVAWQFYGDNVGETVLFGDNDPA 324
           DHARKSAEQA KAI A + G+SV EYDYLPYFYSRSFD++WQFYGDNVGETVLFGDNDPA
Sbjct: 315 DHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPA 374

Query: 323 AAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKEGLDFATKI 147
           ++KPKFGTYW+K+GKV+G FLEGG+ DEN+AIAKVARA+P V DV  LA+EGL FA+KI
Sbjct: 375 SSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 433



 Score = 46.2 bits (108), Expect(2) = 2e-58
 Identities = 20/27 (74%), Positives = 24/27 (88%)
 Frame = -2

Query: 585 VAGVYAIGDVATFPMKLYDEPRRVEHV 505
           V  VYA+GDVATFP+KLY++ RRVEHV
Sbjct: 288 VPDVYAVGDVATFPLKLYNDVRRVEHV 314



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>MDAR4_ARATH (Q93WJ8) Probable monodehydroascorbate reductase, cytoplasmic|
           isoform 4 (EC 1.6.5.4) (MDAR 4)
          Length = 435

 Score =  193 bits (491), Expect(2) = 3e-57
 Identities = 89/119 (74%), Positives = 103/119 (86%)
 Frame = -3

Query: 503 DHARKSAEQAVKAIKAKESGESVAEYDYLPYFYSRSFDVAWQFYGDNVGETVLFGDNDPA 324
           DHARKSAEQAVKAIKA E G S+ EYDYLPYFYSR+FD++WQFYGDNVGE+VLFGDNDP 
Sbjct: 317 DHARKSAEQAVKAIKAAEEGNSIPEYDYLPYFYSRAFDLSWQFYGDNVGESVLFGDNDPE 376

Query: 323 AAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKEGLDFATKI 147
           + KPKFG+YW+K+ KV+G FLEGGS +EN AIAK+ARAQP V  +E L+KEGL FAT I
Sbjct: 377 SPKPKFGSYWIKERKVVGAFLEGGSPEENNAIAKLARAQPSVESLEVLSKEGLSFATNI 435



 Score = 48.1 bits (113), Expect(2) = 3e-57
 Identities = 22/24 (91%), Positives = 23/24 (95%)
 Frame = -2

Query: 576 VYAIGDVATFPMKLYDEPRRVEHV 505
           VYAIGDVATFPMKLY+E RRVEHV
Sbjct: 293 VYAIGDVATFPMKLYNEMRRVEHV 316



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>MDARS_CUCSA (Q42711) Monodehydroascorbate reductase, seedling isozyme (EC|
           1.6.5.4) (MDAR seedling) (Ascorbate free radical
           reductase seedling) (AFR reductase seedling)
          Length = 434

 Score =  182 bits (463), Expect(2) = 1e-53
 Identities = 83/119 (69%), Positives = 100/119 (84%)
 Frame = -3

Query: 503 DHARKSAEQAVKAIKAKESGESVAEYDYLPYFYSRSFDVAWQFYGDNVGETVLFGDNDPA 324
           DH+RKSAEQAVKAIKA E G+++ EYDYLPYFYSRSFD++WQFYGDNVG+ VLFGDN P 
Sbjct: 316 DHSRKSAEQAVKAIKASEEGKAIEEYDYLPYFYSRSFDLSWQFYGDNVGDAVLFGDNSPD 375

Query: 323 AAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKEGLDFATKI 147
           +A  KFG+YW+KDGKV+G FLE GS +EN+AIAKVAR QP V   + L KEG+ FA+K+
Sbjct: 376 SATHKFGSYWIKDGKVVGAFLESGSPEENKAIAKVARIQPSVESSDLLLKEGISFASKV 434



 Score = 47.0 bits (110), Expect(2) = 1e-53
 Identities = 21/27 (77%), Positives = 24/27 (88%)
 Frame = -2

Query: 585 VAGVYAIGDVATFPMKLYDEPRRVEHV 505
           V  VYA+GDVATFP+KLY+E RRVEHV
Sbjct: 289 VPDVYAVGDVATFPLKLYNELRRVEHV 315



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>MDAR1_ARATH (Q9SR59) Probable monodehydroascorbate reductase, cytoplasmic|
           isoform 1 (EC 1.6.5.4) (MDAR 1)
          Length = 441

 Score =  184 bits (467), Expect(2) = 8e-53
 Identities = 84/119 (70%), Positives = 105/119 (88%)
 Frame = -3

Query: 506 SDHARKSAEQAVKAIKAKESGESVAEYDYLPYFYSRSFDVAWQFYGDNVGETVLFGDNDP 327
           +D+ARKSA QAVKAIKA E G+++ +YDYLPYFYSR F ++W+FYG+NVGE+VLFGDNDP
Sbjct: 317 ADNARKSAAQAVKAIKAGEEGKTIPDYDYLPYFYSRFFKLSWEFYGENVGESVLFGDNDP 376

Query: 326 AAAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKEGLDFATK 150
            + KPKFGTYWVKDGKV+GVFLEGG+ +E++AIAKVARAQP V  ++ L++EGL FATK
Sbjct: 377 KSPKPKFGTYWVKDGKVVGVFLEGGTQEEHKAIAKVARAQPSVESLDVLSEEGLSFATK 435



 Score = 42.7 bits (99), Expect(2) = 8e-53
 Identities = 19/26 (73%), Positives = 21/26 (80%)
 Frame = -2

Query: 585 VAGVYAIGDVATFPMKLYDEPRRVEH 508
           V  VYA+GDVATFPMK+Y   RRVEH
Sbjct: 291 VPDVYALGDVATFPMKMYGGTRRVEH 316



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>MDAR_LYCES (Q43497) Monodehydroascorbate reductase (EC 1.6.5.4) (MDAR)|
           (Ascorbate free radical reductase) (AFR reductase)
          Length = 433

 Score =  180 bits (457), Expect(2) = 1e-52
 Identities = 85/119 (71%), Positives = 102/119 (85%)
 Frame = -3

Query: 503 DHARKSAEQAVKAIKAKESGESVAEYDYLPYFYSRSFDVAWQFYGDNVGETVLFGDNDPA 324
           DH+RKSAEQAVKAI A E G+SV EYDYLPYFYSR+FD++WQFYGDNVGETVLFGD DP 
Sbjct: 316 DHSRKSAEQAVKAIFASEQGKSVDEYDYLPYFYSRAFDLSWQFYGDNVGETVLFGDADPN 375

Query: 323 AAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKEGLDFATKI 147
           +A  KFG YW+KDGK++G FLE GS +EN+AIAKVA+ QPP A ++ LA+EG+ FA+KI
Sbjct: 376 SATHKFGQYWIKDGKIVGAFLESGSPEENKAIAKVAKVQPP-ATLDQLAQEGISFASKI 433



 Score = 46.2 bits (108), Expect(2) = 1e-52
 Identities = 20/27 (74%), Positives = 24/27 (88%)
 Frame = -2

Query: 585 VAGVYAIGDVATFPMKLYDEPRRVEHV 505
           V  VYA+GDVATFP+K+Y+E RRVEHV
Sbjct: 289 VPDVYAVGDVATFPLKMYNEIRRVEHV 315



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>MDAR3_ARATH (Q9LFA3) Probable monodehydroascorbate reductase, cytoplasmic|
           isoform 3 (EC 1.6.5.4) (MDAR 3)
          Length = 434

 Score =  178 bits (452), Expect(2) = 1e-51
 Identities = 79/119 (66%), Positives = 101/119 (84%)
 Frame = -3

Query: 503 DHARKSAEQAVKAIKAKESGESVAEYDYLPYFYSRSFDVAWQFYGDNVGETVLFGDNDPA 324
           DH+RKSAEQAVKAIKA E G +V EYDYLP+FYSRSFD++WQFYGDNVG++VLFGD++P+
Sbjct: 316 DHSRKSAEQAVKAIKAAEGGAAVEEYDYLPFFYSRSFDLSWQFYGDNVGDSVLFGDSNPS 375

Query: 323 AAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKEGLDFATKI 147
             KP+FG YWV+ GKV+G F+EGGS DEN+A+AKVA+A+P    ++ L K+G+ FA KI
Sbjct: 376 NPKPRFGAYWVQGGKVVGAFMEGGSGDENKALAKVAKARPSAESLDELVKQGISFAAKI 434



 Score = 44.7 bits (104), Expect(2) = 1e-51
 Identities = 19/27 (70%), Positives = 23/27 (85%)
 Frame = -2

Query: 585 VAGVYAIGDVATFPMKLYDEPRRVEHV 505
           V  VYA+GDVATFP+K+Y + RRVEHV
Sbjct: 289 VPDVYAVGDVATFPLKMYGDVRRVEHV 315



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>MDARF_CUCSA (P83966) Monodehydroascorbate reductase, fruit isozyme (EC 1.6.5.4)|
           (MDAR fruit) (Ascorbate free radical reductase fruit)
           (AFR reductase fruit) (Fragments)
          Length = 166

 Score =  111 bits (278), Expect(2) = 6e-32
 Identities = 57/89 (64%), Positives = 66/89 (74%)
 Frame = -3

Query: 440 SVAEYDYLPYFYSRSFDVAWQFYGDNVGETVLFGDNDPAAAKPKFGTYWVKDGKVIGVFL 261
           S+ EYDYLPYFYSR+F++AWQFYGDNVGETVLF DN        FGTYW+   KV+GVFL
Sbjct: 93  SIEEYDYLPYFYSRTFNLAWQFYGDNVGETVLFPDN--------FGTYWI---KVVGVFL 141

Query: 260 EGGSADENQAIAKVARAQPPVADVEALAK 174
           EGG+ DE     KVAR QPPV  ++ LAK
Sbjct: 142 EGGTPDE----YKVARVQPPVESLDQLAK 166



 Score = 45.4 bits (106), Expect(2) = 6e-32
 Identities = 19/27 (70%), Positives = 24/27 (88%)
 Frame = -2

Query: 585 VAGVYAIGDVATFPMKLYDEPRRVEHV 505
           +  VYA+GDVAT+P+KLY+E RRVEHV
Sbjct: 61  IPDVYAVGDVATYPLKLYNELRRVEHV 87



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>MDAR2_ARATH (Q9LK94) Probable monodehydroascorbate reductase, cytoplasmic|
           isoform 2 (EC 1.6.5.4) (MDAR 2)
          Length = 488

 Score =  104 bits (260), Expect(2) = 2e-29
 Identities = 55/117 (47%), Positives = 73/117 (62%), Gaps = 1/117 (0%)
 Frame = -3

Query: 503 DHARKSAEQAVKAIKAKESGESVAEYDYLPYFYSRSFDVAWQFYGDNVGETVLFGDNDPA 324
           D ARKSA  AV AI          ++DYLP+FYSR F  +WQFYGD  G+ V FG+ +  
Sbjct: 315 DSARKSARHAVSAIM---DPIKTGDFDYLPFFYSRVFAFSWQFYGDPTGDVVHFGEYEDG 371

Query: 323 AAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVA-DVEALAKEGLDFA 156
            +   FG YWVK G ++G FLEGG+ +E + I+K  + +P V  D+E L +EGL FA
Sbjct: 372 KS---FGAYWVKKGHLVGSFLEGGTKEEYETISKATQLKPAVTIDLEELEREGLGFA 425



 Score = 43.9 bits (102), Expect(2) = 2e-29
 Identities = 20/34 (58%), Positives = 25/34 (73%)
 Frame = -2

Query: 582 AGVYAIGDVATFPMKLYDEPRRVEHVGPCSEIGR 481
           + VYAIGDVATFP+KL+ E RR+EHV    +  R
Sbjct: 289 SSVYAIGDVATFPVKLFGEMRRLEHVDSARKSAR 322



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>MDARP_ARATH (P92947) Monodehydroascorbate reductase, chloroplast precursor (EC|
           1.6.5.4) (MDAR)
          Length = 493

 Score = 97.8 bits (242), Expect(2) = 7e-27
 Identities = 53/126 (42%), Positives = 76/126 (60%), Gaps = 7/126 (5%)
 Frame = -3

Query: 503 DHARKSAEQAVKAIKAKESGESVAEYDYLPYFYSRSFD-------VAWQFYGDNVGETVL 345
           DHAR+SA+  VK++    +      YDYLPYFYSR F+       V WQF+GDNVGETV 
Sbjct: 370 DHARRSAQHCVKSLLTAHTDT----YDYLPYFYSRVFEYEGSPRKVWWQFFGDNVGETVE 425

Query: 344 FGDNDPAAAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKEGL 165
            G+ DP     K  T+W++ G++ GV +E GS +E Q + K+AR+QP V   +  +   +
Sbjct: 426 VGNFDP-----KIATFWIESGRLKGVLVESGSPEEFQLLPKLARSQPLVDKAKLASASSV 480

Query: 164 DFATKI 147
           + A +I
Sbjct: 481 EEALEI 486



 Score = 42.4 bits (98), Expect(2) = 7e-27
 Identities = 17/25 (68%), Positives = 21/25 (84%)
 Frame = -2

Query: 579 GVYAIGDVATFPMKLYDEPRRVEHV 505
           G++AIGDVA FP+K+YD   RVEHV
Sbjct: 345 GIFAIGDVAAFPLKIYDRMTRVEHV 369



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>ALG14_ASPFU (Q4WNB5) UDP-N-acetylglucosamine transferase subunit alg14 (EC|
           2.4.1.-) (Asparagine linked glycosylation protein 14)
          Length = 228

 Score = 30.4 bits (67), Expect = 3.9
 Identities = 15/45 (33%), Positives = 23/45 (51%)
 Frame = -3

Query: 341 GDNDPAAAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQ 207
           GDN  AA   +F T W+K    +     G ++ E+ A+  V RA+
Sbjct: 25  GDNFSAARAVEFETEWLKQSPKLSFPANGSNSTESYAVVTVPRAR 69



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>K6PP_MOUSE (Q9WUA3) 6-phosphofructokinase type C (EC 2.7.1.11)|
           (Phosphofructokinase 1) (Phosphohexokinase)
           (Phosphofructo-1-kinase isozyme C) (PFK-C)
          Length = 784

 Score = 30.4 bits (67), Expect = 3.9
 Identities = 17/43 (39%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
 Frame = -3

Query: 338 DNDPAAAKPKFGTYWVKDGKVIGVFLEGGSAD-ENQAIAKVAR 213
           D+  ++A PK+  +   DGK IGV   GG A   N A+  V R
Sbjct: 5   DSSSSSAYPKYLEHLSGDGKAIGVLTSGGDAQGMNAAVRAVVR 47



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>EFTU_MYCPE (Q8EX18) Elongation factor Tu (EF-Tu)|
          Length = 394

 Score = 29.3 bits (64), Expect = 8.7
 Identities = 19/54 (35%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
 Frame = -3

Query: 491 KSAEQAVKAIKAKESGESVAEYD-YLPYFYSRSFDVAWQFYGDNVGETVLFGDN 333
           K  E  + A+K +E G      + Y P FY R+ DV  Q   D   E +  GDN
Sbjct: 303 KQFEAEIYALKKEEGGRHTPVLNGYRPQFYFRTTDVTGQITLDKGVEMINPGDN 356



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>6PGL_PSEAE (Q9X2N2) 6-phosphogluconolactonase (EC 3.1.1.31) (6PGL)|
          Length = 238

 Score = 29.3 bits (64), Expect = 8.7
 Identities = 14/36 (38%), Positives = 24/36 (66%)
 Frame = -3

Query: 263 LEGGSADENQAIAKVARAQPPVADVEALAKEGLDFA 156
           L    A++ QA+  V+  + PVA +EAL++E LD++
Sbjct: 35  LRSALAEQGQALLVVSGGRSPVAFLEALSEEPLDWS 70


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 67,660,961
Number of Sequences: 219361
Number of extensions: 1152809
Number of successful extensions: 4522
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 4380
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4514
length of database: 80,573,946
effective HSP length: 106
effective length of database: 57,321,680
effective search space used: 5044307840
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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