ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd11e21
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1NEP2_NEPGR (Q766C2) Aspartic proteinase nepenthesin-2 precursor ... 89 2e-17
2NEP1_NEPGR (Q766C3) Aspartic proteinase nepenthesin-1 precursor ... 83 9e-16
3NEP2_NEPDI (P69477) Aspartic proteinase nepenthesin-2 (EC 3.4.23... 43 8e-04
4AROA_CORGL (Q9Z470) 3-phosphoshikimate 1-carboxyvinyltransferase... 36 0.096
5YPS3_YEAST (Q12303) Aspartic proteinase yapsin-3 precursor (EC 3... 34 0.47
6SGS3_DROME (P02840) Salivary glue protein Sgs-3 precursor 33 1.1
7CARP1_RHINI (P10602) Rhizopuspepsin-1 precursor (EC 3.4.23.21) (... 32 2.4
8BACE2_HUMAN (Q9Y5Z0) Beta-secretase 2 precursor (EC 3.4.23.45) (... 30 5.2
9YHU3_YEAST (P38844) Protein YHR143W precursor 30 9.0

>NEP2_NEPGR (Q766C2) Aspartic proteinase nepenthesin-2 precursor (EC 3.4.23.-)|
           (Nepenthesin-II)
          Length = 438

 Score = 88.6 bits (218), Expect = 2e-17
 Identities = 57/167 (34%), Positives = 82/167 (49%), Gaps = 12/167 (7%)
 Frame = -1

Query: 651 MLRSQQSPTFYGVLLQGIKVGGEQLSVAPSVF------SAGSVMDSGTIITRLPPTAYTA 490
           ++ S  +PT+Y + LQGI VGG+ L +  S F      + G ++DSGT +T LP  AY A
Sbjct: 271 LIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNA 330

Query: 489 LKTAFKDGMKQYPTAPARSILDTCFDF-SGQDNVTIPTVALVFDGGKVVDLDANGIIFGS 313
           +  AF D +       + S L TCF   S    V +P +++ FDGG V++L    I+   
Sbjct: 331 VAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGG-VLNLGEQNILISP 389

Query: 312 -----CLAFXXXXXXXXXGIIGNVQQRTLEVLYDVGQSVFGFKSNAC 187
                CLA           I GN+QQ+  +VLYD+      F    C
Sbjct: 390 AEGVICLAM-GSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435



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>NEP1_NEPGR (Q766C3) Aspartic proteinase nepenthesin-1 precursor (EC 3.4.23.-)|
           (Nepenthesin-I)
          Length = 437

 Score = 82.8 bits (203), Expect = 9e-16
 Identities = 56/167 (33%), Positives = 79/167 (47%), Gaps = 12/167 (7%)
 Frame = -1

Query: 651 MLRSQQSPTFYGVLLQGIKVGGEQLSVAPSVFS-------AGSVMDSGTIITRLPPTAYT 493
           +++S Q PTFY + L G+ VG  +L + PS F+        G ++DSGT +T     AY 
Sbjct: 270 LIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQ 329

Query: 492 ALKTAFKDGMKQYPTAPARSILDTCFDF-SGQDNVTIPTVALVFDGGKVVDLDANGIIFG 316
           +++  F   +       + S  D CF   S   N+ IPT  + FDGG +     N  I  
Sbjct: 330 SVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDLELPSENYFISP 389

Query: 315 S----CLAFXXXXXXXXXGIIGNVQQRTLEVLYDVGQSVFGFKSNAC 187
           S    CLA           I GN+QQ+ + V+YD G SV  F S  C
Sbjct: 390 SNGLICLAMGSSSQGMS--IFGNIQQQNMLVVYDTGNSVVSFASAQC 434



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>NEP2_NEPDI (P69477) Aspartic proteinase nepenthesin-2 (EC 3.4.23.-)|
           (Nepenthesin-II) (Fragments)
          Length = 178

 Score = 43.1 bits (100), Expect = 8e-04
 Identities = 34/103 (33%), Positives = 47/103 (45%), Gaps = 3/103 (2%)
 Frame = -1

Query: 618 GVLLQGIKVGGEQLSVAPSVFSAGSVM--DSGTIITRLPPTAYTALKTAFKDGMKQYPTA 445
           G   QG     +  SV    F  G  +  DSGT +T LP  AY A+  AF D +      
Sbjct: 69  GSSTQGYMAXEDGSSVPNIAFGCGDNLQIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVD 128

Query: 444 PARSILDTCF-DFSGQDNVTIPTVALVFDGGKVVDLDANGIIF 319
            + S L TCF + S    V +P +++  DGG + DL    + F
Sbjct: 129 ESSSGLSTCFQEPSDGSTVQVPEISMQ-DGGVLNDLQNLAVSF 170



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>AROA_CORGL (Q9Z470) 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19)|
           (5-enolpyruvylshikimate-3-phosphate synthase) (EPSP
           synthase) (EPSPS)
          Length = 430

 Score = 36.2 bits (82), Expect = 0.096
 Identities = 33/92 (35%), Positives = 44/92 (47%), Gaps = 6/92 (6%)
 Frame = -1

Query: 585 EQLSVAPSVFSAGSVMD--SGTIITRLPPTAYTALKTAFKDGMKQYPTAPARSILDTCFD 412
           ++  V P   SAGSV    +GT++  LPP A  A      DG  Q    P  SILD    
Sbjct: 81  DRYRVEPGQLSAGSVECGLAGTVMRFLPPVAAFADGPVHFDGDPQARVRPMTSILDALRS 140

Query: 411 FSGQ-DNVTIP---TVALVFDGGKVVDLDANG 328
              + DN  +P       V +GG VV++DA+G
Sbjct: 141 LGVEVDNNNLPFTVNAGEVPEGG-VVEIDASG 171



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>YPS3_YEAST (Q12303) Aspartic proteinase yapsin-3 precursor (EC 3.4.23.-)|
          Length = 508

 Score = 33.9 bits (76), Expect = 0.47
 Identities = 21/84 (25%), Positives = 37/84 (44%), Gaps = 6/84 (7%)
 Frame = -1

Query: 636 QSPTFYGVLLQGIKVGGEQLSVAPSVFSAGSVMDSGTIITRLPPTAYTALKTAFKD---- 469
           Q P  + V LQG+ +  ++ ++  +     +++DSGT +T LP  A   L  +       
Sbjct: 255 QHPVAFDVTLQGLGLQTDKRNITLTTTKLPALLDSGTTLTYLPSQAVALLAKSLNASYSK 314

Query: 468 --GMKQYPTAPARSILDTCFDFSG 403
             G  +Y    + +     FDF G
Sbjct: 315 TLGYYEYTCPSSDNKTSVAFDFGG 338



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>SGS3_DROME (P02840) Salivary glue protein Sgs-3 precursor|
          Length = 307

 Score = 32.7 bits (73), Expect = 1.1
 Identities = 21/72 (29%), Positives = 30/72 (41%)
 Frame = +2

Query: 221 PTS*STSRVLCCTXXXXXXXXXXXXXXNARQLPNMIPLASRSTTLPPSNTKATVGMVTLS 400
           PT  ST++  C T                 QLP   P  +++TT  P+ TKAT    T +
Sbjct: 58  PTQQSTTQPPCTTSKPTTPKQTTT------QLPCTTPTTTKATTTKPTTTKATTTKATTT 111

Query: 401 WPLKSKHVSRML 436
            P  +K  +  L
Sbjct: 112 KPTTTKQTTTQL 123



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>CARP1_RHINI (P10602) Rhizopuspepsin-1 precursor (EC 3.4.23.21) (Aspartate|
           protease)
          Length = 389

 Score = 31.6 bits (70), Expect = 2.4
 Identities = 25/101 (24%), Positives = 44/101 (43%), Gaps = 5/101 (4%)
 Frame = -1

Query: 633 SPTFYGVLLQGIKVGGEQLSVAPSVFSAGSVMDSGTIITRLPPTAYTALKTAF---KDGM 463
           S  F+GV ++  K+GG  +S      S  +++D+GT +  LP      +  ++    +G 
Sbjct: 256 SEGFWGVTVKSTKIGGTTVSA-----SFDAILDTGTTLLLLPDDVAAKVARSYGASDNGD 310

Query: 462 KQYPTAPARSILDTCFDFSGQDNVTIPTVALVF--DGGKVV 346
             Y      S L       G     +P+ +L+F  DG K +
Sbjct: 311 GTYSITCDTSKLQPLVFTLGSSTFEVPSDSLIFEKDGNKCI 351



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>BACE2_HUMAN (Q9Y5Z0) Beta-secretase 2 precursor (EC 3.4.23.45) (Beta-site|
           APP-cleaving enzyme 2) (Aspartyl protease 1) (Asp 1)
           (ASP1) (Membrane-associated aspartic protease 1)
           (Memapsin-1) (Down region aspartic protease)
          Length = 518

 Score = 30.4 bits (67), Expect = 5.2
 Identities = 14/50 (28%), Positives = 31/50 (62%), Gaps = 1/50 (2%)
 Frame = -1

Query: 624 FYGVLLQGIKVGGEQLSVAPSVFSAG-SVMDSGTIITRLPPTAYTALKTA 478
           +Y + +  +++GG+ L++    ++A  +++DSGT + RLP   + A+  A
Sbjct: 273 YYQIEILKLEIGGQSLNLDCREYNADKAIVDSGTTLLRLPQKVFDAVVEA 322



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>YHU3_YEAST (P38844) Protein YHR143W precursor|
          Length = 325

 Score = 29.6 bits (65), Expect = 9.0
 Identities = 22/90 (24%), Positives = 41/90 (45%), Gaps = 1/90 (1%)
 Frame = +2

Query: 332 LASRSTTLPPSNTKATVGMVTLSWPLKSKHVSRMLLAGAVGYCFM-PSLNAVLSAVYAVG 508
           +A  ++TL PS++  T    T S PL+S     + ++ + G C++    +   S VY   
Sbjct: 243 IARTTSTLVPSSSVDTTSRATTSMPLESSSTQSISVSSSDGTCYVFYDDDDYYSTVY--- 299

Query: 509 GSRVMMVPESITDPAEKTEGATLSCSPPTL 598
                     +T+P++  + AT   S  T+
Sbjct: 300 ----------LTNPSQSVDAATTITSTNTI 319


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 75,084,406
Number of Sequences: 219361
Number of extensions: 1238439
Number of successful extensions: 4215
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 4106
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4211
length of database: 80,573,946
effective HSP length: 108
effective length of database: 56,882,958
effective search space used: 6769072002
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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