ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bags9c23
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1MD2L2_HUMAN (Q9UI95) Mitotic spindle assembly checkpoint protein... 80 5e-15
2MD2L2_MOUSE (Q9D752) Mitotic spindle assembly checkpoint protein... 80 7e-15
3MD2L1_MOUSE (Q9Z1B5) Mitotic spindle assembly checkpoint protein... 46 1e-04
4MAD2_YEAST (P40958) Mitotic spindle checkpoint component MAD2 (M... 40 0.006
5NPHP1_CAEEL (O17972) Nephrocystin-1-like protein (Nephronophthis... 32 1.2
6DMAW2_CLAPU (Q9C141) Tryptophan dimethylallyltransferase 2 (EC 2... 32 1.6
7Y330_MYCPN (P75455) Hypothetical protein MG237 homolog (F10_orf294) 31 2.7
8CP3AP_MOUSE (O09158) Cytochrome P450 3A25 (EC 1.14.14.1) (CYPIII... 31 3.6
9PUB2_SCHPO (Q9UTG2) E3 ubiquitin--protein ligase pub2 (EC 6.3.2.-) 30 4.7
10ADEC1_AGRT5 (Q7CXF0) Adenine deaminase 1 (EC 3.5.4.2) (Adenase 1... 30 6.1
11TGM2_PAGMA (P52181) Protein-glutamine gamma-glutamyltransferase ... 30 8.0
12DMAW1_CLAPU (Q6X2E0) Tryptophan dimethylallyltransferase 1 (EC 2... 30 8.0

>MD2L2_HUMAN (Q9UI95) Mitotic spindle assembly checkpoint protein MAD2B|
           (MAD2-like 2) (hREV7)
          Length = 211

 Score = 80.1 bits (196), Expect = 5e-15
 Identities = 37/88 (42%), Positives = 59/88 (67%)
 Frame = +3

Query: 210 IAQVIVEFLEVAVSCIVFLKGFYPARAFERRRYMNIVVQKAVHPQLADYIHSVTTGLLPF 389
           +A V+ EFLEVAV  I++++  YP   F++R+  N+ VQ + HP+L  YI      + P 
Sbjct: 15  VADVLCEFLEVAVHLILYVREVYPVGIFQKRKKYNVPVQMSCHPELNQYIQDTLHCVKPL 74

Query: 390 IQKGLVERVVVIFHDKDNVPLEKFVFKL 473
           ++K  VE+VVV+  DK++ P+EKFVF++
Sbjct: 75  LEKNDVEKVVVVILDKEHRPVEKFVFEI 102



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>MD2L2_MOUSE (Q9D752) Mitotic spindle assembly checkpoint protein MAD2B|
           (MAD2-like 2)
          Length = 211

 Score = 79.7 bits (195), Expect = 7e-15
 Identities = 37/88 (42%), Positives = 59/88 (67%)
 Frame = +3

Query: 210 IAQVIVEFLEVAVSCIVFLKGFYPARAFERRRYMNIVVQKAVHPQLADYIHSVTTGLLPF 389
           +A V+ EFLEVAV  I++++  YP   F++R+  N+ VQ + HP+L  YI      + P 
Sbjct: 15  VADVLSEFLEVAVHLILYVREVYPVGIFQKRKKYNVPVQMSCHPELNQYIQDTLHCVKPL 74

Query: 390 IQKGLVERVVVIFHDKDNVPLEKFVFKL 473
           ++K  VE+VVV+  DK++ P+EKFVF++
Sbjct: 75  LEKNDVEKVVVVILDKEHRPVEKFVFEI 102



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>MD2L1_MOUSE (Q9Z1B5) Mitotic spindle assembly checkpoint protein MAD2A|
           (MAD2-like 1)
          Length = 205

 Score = 45.8 bits (107), Expect = 1e-04
 Identities = 23/93 (24%), Positives = 51/93 (54%), Gaps = 1/93 (1%)
 Frame = +3

Query: 213 AQVIVEFLEVAVSCIVFLKGFYPARAFERRRYMNIVVQKAVHPQLADYIHSVTTGLLPFI 392
           A+++ EF    ++ I++ +G YP+  F R +   + +     P+L  Y+++V   L  ++
Sbjct: 17  AEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLTTTDPELIKYLNNVVEQLKEWL 76

Query: 393 QKGLVER-VVVIFHDKDNVPLEKFVFKLAVNQS 488
            K  V++ VVVI + +    LE++ F +  +++
Sbjct: 77  YKCSVQKLVVVISNIESGEVLERWQFDIECDKT 109



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>MAD2_YEAST (P40958) Mitotic spindle checkpoint component MAD2 (Mitotic MAD2|
           protein)
          Length = 196

 Score = 40.0 bits (92), Expect = 0.006
 Identities = 19/75 (25%), Positives = 39/75 (52%)
 Frame = +3

Query: 216 QVIVEFLEVAVSCIVFLKGFYPARAFERRRYMNIVVQKAVHPQLADYIHSVTTGLLPFIQ 395
           + + EF E +++ I++ +G YPA  F   +  ++ + K    +L DYI  +   +  ++ 
Sbjct: 12  RTVTEFFEYSINSILYQRGVYPAEDFVTVKKYDLTLLKTHDDELKDYIRKILLQVHRWLL 71

Query: 396 KGLVERVVVIFHDKD 440
            G   ++V+   DKD
Sbjct: 72  GGKCNQLVLCIVDKD 86



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>NPHP1_CAEEL (O17972) Nephrocystin-1-like protein (Nephronophthisis homolog)|
          Length = 682

 Score = 32.3 bits (72), Expect = 1.2
 Identities = 27/113 (23%), Positives = 53/113 (46%), Gaps = 12/113 (10%)
 Frame = +3

Query: 156 VIYLNWAMDRKNQTPQGQIAQVIVEFLEVAVS----------CIVFLKGFYPARAFERRR 305
           V+YL+  +  K + P+    +V+V+ ++V              IVF   + P   F RRR
Sbjct: 487 VLYLDDEVMTKMKLPEASKRRVLVQVMDVPKDKVSYVDSLPDVIVFNALYLPFFHFYRRR 546

Query: 306 YMNIVVQKAVHPQLADYIHSVTTGLLPFI--QKGLVERVVVIFHDKDNVPLEK 458
              I+++   +P  A++I      + PF+  Q  +++ ++ I+  K  V  +K
Sbjct: 547 AGTILIRDNRNPLSAEFISDPLLSVFPFVCDQHDIMDIMLKIWKTKKKVLAKK 599



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>DMAW2_CLAPU (Q9C141) Tryptophan dimethylallyltransferase 2 (EC 2.5.1.34) (DMATS|
           2) (All-trans-hexaprenyl-diphosphate synthase 2)
           (L-tryptophan dimethylallyl transferase 2)
          Length = 448

 Score = 32.0 bits (71), Expect = 1.6
 Identities = 18/57 (31%), Positives = 29/57 (50%), Gaps = 3/57 (5%)
 Frame = +3

Query: 231 FLEVAVSCIVFLKGFYPARAFERRRYMNIVVQ---KAVHPQLADYIHSVTTGLLPFI 392
           F ++A    VFL+  YP   FE   Y++ +V    +   P L+ Y+H+  TG  P +
Sbjct: 374 FADMAKKYRVFLQDSYPYHDFESLNYLHSLVSFSYRRNKPYLSVYLHTFETGRWPVV 430



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>Y330_MYCPN (P75455) Hypothetical protein MG237 homolog (F10_orf294)|
          Length = 294

 Score = 31.2 bits (69), Expect = 2.7
 Identities = 16/60 (26%), Positives = 32/60 (53%)
 Frame = +3

Query: 336 HPQLADYIHSVTTGLLPFIQKGLVERVVVIFHDKDNVPLEKFVFKLAVNQSYGFRVRGVQ 515
           +P   + IH +      F++  L++R+  +  D + + L++F F  A+NQ  GF+   V+
Sbjct: 119 YPNKLNMIHYLLPKTKAFVKPHLLQRLQFVLTDSELLELKRFSFFQALNQIPGFQGEQVE 178



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>CP3AP_MOUSE (O09158) Cytochrome P450 3A25 (EC 1.14.14.1) (CYPIIIA25)|
          Length = 503

 Score = 30.8 bits (68), Expect = 3.6
 Identities = 23/89 (25%), Positives = 42/89 (47%)
 Frame = +3

Query: 120 KQQPESSKLQNLVIYLNWAMDRKNQTPQGQIAQVIVEFLEVAVSCIVFLKGFYPARAFER 299
           K++  +S  +N V +L   M+ +N   +GQ +Q  +  LE+A   ++F+ G Y A +   
Sbjct: 257 KKERLASNQKNRVDFLQLMMNTQNS--KGQESQKALSDLEMAAQAVIFIFGGYDATS--- 311

Query: 300 RRYMNIVVQKAVHPQLADYIHSVTTGLLP 386
                I+ + A HP +   +       LP
Sbjct: 312 TSISLIMYELATHPDVQKKLQDEIDRTLP 340



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>PUB2_SCHPO (Q9UTG2) E3 ubiquitin--protein ligase pub2 (EC 6.3.2.-)|
          Length = 671

 Score = 30.4 bits (67), Expect = 4.7
 Identities = 13/24 (54%), Positives = 16/24 (66%)
 Frame = -1

Query: 469 LNTNFSSGTLSLSWKMTTTLSTSP 398
           + TN +SGTL LSW  T  L+ SP
Sbjct: 41  IKTNVASGTLRLSWGFTQKLTVSP 64



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>ADEC1_AGRT5 (Q7CXF0) Adenine deaminase 1 (EC 3.5.4.2) (Adenase 1) (Adenine|
           aminase 1)
          Length = 575

 Score = 30.0 bits (66), Expect = 6.1
 Identities = 11/25 (44%), Positives = 17/25 (68%)
 Frame = -3

Query: 368 HRVDIVSKLGVHGFLDNYIHVPSPL 294
           H +DI  K+ V GF+D ++H+ S L
Sbjct: 69  HEIDISGKIVVPGFIDTHLHIESSL 93



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>TGM2_PAGMA (P52181) Protein-glutamine gamma-glutamyltransferase 2 (EC|
           2.3.2.13) (Tissue transglutaminase) (TGase C) (TGC)
           (TG(C)) (Tranglutaminase-2)
          Length = 695

 Score = 29.6 bits (65), Expect = 8.0
 Identities = 12/41 (29%), Positives = 22/41 (53%)
 Frame = -1

Query: 481 LTASLNTNFSSGTLSLSWKMTTTLSTSPFCMKGRRPVVTEW 359
           +TA +N+N   G L+  W+   T   +P+   G  P++ +W
Sbjct: 219 ITAMVNSNGDRGVLTGRWEEPYTDGVAPYRWTGSVPILQQW 259



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>DMAW1_CLAPU (Q6X2E0) Tryptophan dimethylallyltransferase 1 (EC 2.5.1.34) (DMATS|
           1) (All-trans-hexaprenyl-diphosphate synthase 1)
           (L-tryptophan dimethylallyl transferase 1)
          Length = 448

 Score = 29.6 bits (65), Expect = 8.0
 Identities = 17/55 (30%), Positives = 28/55 (50%), Gaps = 3/55 (5%)
 Frame = +3

Query: 231 FLEVAVSCIVFLKGFYPARAFERRRYMNIVVQ---KAVHPQLADYIHSVTTGLLP 386
           F ++A +  VFL+  YP   FE   Y++  +    +   P L+ Y+H+  TG  P
Sbjct: 374 FDDMAKNYRVFLQDSYPYHDFESLNYLHAYISFSYRRNKPYLSVYLHTFETGRWP 428


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 97,533,976
Number of Sequences: 219361
Number of extensions: 2053261
Number of successful extensions: 4767
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 4619
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4766
length of database: 80,573,946
effective HSP length: 108
effective length of database: 56,882,958
effective search space used: 6029593548
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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