ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bah63i24
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1UVRD_ECOLI (P03018) DNA helicase II (EC 3.6.1.-) 35 0.24
2YG1F_YEAST (P53214) Hypothetical 57.5 kDa protein in VMA7-RPS25A... 34 0.32
3UVRD_SALTY (Q05311) DNA helicase II (EC 3.6.1.-) 34 0.41
4UVRD_HAEIN (Q02322) DNA helicase II (EC 3.6.1.-) 33 0.92
5YM96_YEAST (Q04893) Hypothetical 113.1 kDa protein in PRE5-FET4 ... 32 1.6
6CH60_LACZE (O32847) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 32 1.6
7SRS2_YEAST (P12954) ATP-dependent DNA helicase SRS2 (EC 3.6.1.-) 32 1.6
8PCRA_MYCTU (P0A5A3) ATP-dependent DNA helicase pcrA (EC 3.6.1.-) 32 2.0
9PCRA_MYCBO (P0A5A4) ATP-dependent DNA helicase pcrA (EC 3.6.1.-) 32 2.0
10PCRA_MYCLE (Q9CD72) ATP-dependent DNA helicase pcrA (EC 3.6.1.-) 31 2.7
11NOTC2_HUMAN (Q04721) Neurogenic locus notch homolog protein 2 pr... 31 2.7
12NOTC1_RAT (Q07008) Neurogenic locus notch homolog protein 1 prec... 31 3.5
13GPMA2_LACPL (Q88T35) 2,3-bisphosphoglycerate-dependent phosphogl... 31 3.5
14UBR1_YEAST (P19812) Ubiquitin-protein ligase E3 component N-reco... 30 4.6
15UVRD_RICPR (Q9ZD95) Probable DNA helicase II homolog (EC 3.6.1.-) 30 4.6
16YHU3_YEAST (P38844) Protein YHR143W precursor 30 6.0
17NOTC2_RAT (Q9QW30) Neurogenic locus notch homolog protein 2 prec... 30 6.0
18NOTC2_MOUSE (O35516) Neurogenic locus notch homolog protein 2 pr... 30 6.0
19NOTCH_XENLA (P21783) Neurogenic locus notch protein homolog prec... 30 7.8
20AGA1_YEAST (P32323) A-agglutinin attachment subunit precursor 30 7.8
21YLU2_PICAN (P34735) Hypothetical protein in LEU2 3'region (Fragm... 30 7.8

>UVRD_ECOLI (P03018) DNA helicase II (EC 3.6.1.-)|
          Length = 720

 Score = 34.7 bits (78), Expect = 0.24
 Identities = 21/34 (61%), Positives = 24/34 (70%), Gaps = 3/34 (8%)
 Frame = +2

Query: 542 LHSLNDKQREASCSDVAVP---LMIVAGPGSGKT 634
           L SLNDKQREA    VA P   L+++AG GSGKT
Sbjct: 7   LDSLNDKQREA----VAAPRSNLLVLAGAGSGKT 36



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>YG1F_YEAST (P53214) Hypothetical 57.5 kDa protein in VMA7-RPS25A intergenic|
           region
          Length = 551

 Score = 34.3 bits (77), Expect = 0.32
 Identities = 38/145 (26%), Positives = 59/145 (40%), Gaps = 6/145 (4%)
 Frame = -2

Query: 453 MASTSPKNASTTSPPSSAMEITGGKSTYNATEPXXXXXXXXXSWLIAEDVA*CSWFI--- 283
           +ASTS   AS+T   SS++ I+   ST+  +              I+  V+  S ++   
Sbjct: 152 VASTSSTVASSTLSTSSSLVISTSSSTFTFSSESSSSLISSS---ISTSVSTSSVYVPSS 208

Query: 282 --SEDMAWCSLLMALTSQKVSSNLTLSSAMDIAGRSESSPQDSTKLXXXXXXXXXXSWRL 109
             S   +  S L + +    SS+ TL S       S SS   S+             + L
Sbjct: 209 STSSPPSSSSELTSSSYSSSSSSSTLFSYSSSFSSSSSSSSSSSSSSSSSSSSSSSYFTL 268

Query: 108 AAG*ASH-SSKMSYASFQNASSSVP 37
           +   +S   S  SY SF ++SSS P
Sbjct: 269 STSSSSSIYSSSSYPSFSSSSSSNP 293



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>UVRD_SALTY (Q05311) DNA helicase II (EC 3.6.1.-)|
          Length = 720

 Score = 33.9 bits (76), Expect = 0.41
 Identities = 20/34 (58%), Positives = 24/34 (70%), Gaps = 3/34 (8%)
 Frame = +2

Query: 542 LHSLNDKQREASCSDVAVP---LMIVAGPGSGKT 634
           L SLNDKQREA    VA P   ++++AG GSGKT
Sbjct: 7   LDSLNDKQREA----VAAPRSNMLVLAGAGSGKT 36



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>UVRD_HAEIN (Q02322) DNA helicase II (EC 3.6.1.-)|
          Length = 727

 Score = 32.7 bits (73), Expect = 0.92
 Identities = 20/36 (55%), Positives = 23/36 (63%), Gaps = 3/36 (8%)
 Frame = +2

Query: 536 EYLHSLNDKQREASCSDVAVPL---MIVAGPGSGKT 634
           E L  LNDKQRE     VA PL   +++AG GSGKT
Sbjct: 6   ELLDGLNDKQRER----VAAPLGNHLVLAGAGSGKT 37



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>YM96_YEAST (Q04893) Hypothetical 113.1 kDa protein in PRE5-FET4 intergenic|
           region
          Length = 1140

 Score = 32.0 bits (71), Expect = 1.6
 Identities = 24/96 (25%), Positives = 47/96 (48%)
 Frame = -2

Query: 447 STSPKNASTTSPPSSAMEITGGKSTYNATEPXXXXXXXXXSWLIAEDVA*CSWFISEDMA 268
           S+SPK +S+ S       +T   +T +A++          S L++   +  S  ISE + 
Sbjct: 194 SSSPKISSSGSA------VTSVGTTSDASKEVFSSSTSDVSSLLSSTSSPASSTISETLP 247

Query: 267 WCSLLMALTSQKVSSNLTLSSAMDIAGRSESSPQDS 160
           + S ++++TS  VSS    +++  ++  + SS   S
Sbjct: 248 FSSTILSITSSPVSSEAPSATSSSVSSEASSSTSSS 283



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>CH60_LACZE (O32847) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (HSP60)|
          Length = 544

 Score = 32.0 bits (71), Expect = 1.6
 Identities = 19/57 (33%), Positives = 27/57 (47%), Gaps = 2/57 (3%)
 Frame = +2

Query: 248 AISKEHHAISSEMNQEHYATSSAINQDE--IEEVYVEDGSVALYVDFPPVISIAEEG 412
           A+ K   A  +E+ +  Y    A+N     +EE YV  G  AL    P V ++ EEG
Sbjct: 375 AVVKVGAATETELKERKYRIEDALNATRAAVEEGYVAGGGTALVDVLPAVAALKEEG 431



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>SRS2_YEAST (P12954) ATP-dependent DNA helicase SRS2 (EC 3.6.1.-)|
          Length = 1174

 Score = 32.0 bits (71), Expect = 1.6
 Identities = 14/28 (50%), Positives = 19/28 (67%)
 Frame = +2

Query: 551 LNDKQREASCSDVAVPLMIVAGPGSGKT 634
           LN +QR A+  D    L ++AGPG+GKT
Sbjct: 15  LNTQQRAAALFDYTRGLQVIAGPGTGKT 42



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>PCRA_MYCTU (P0A5A3) ATP-dependent DNA helicase pcrA (EC 3.6.1.-)|
          Length = 771

 Score = 31.6 bits (70), Expect = 2.0
 Identities = 17/33 (51%), Positives = 23/33 (69%)
 Frame = +2

Query: 536 EYLHSLNDKQREASCSDVAVPLMIVAGPGSGKT 634
           + L  LN +QR+A   + + PL+IVAG GSGKT
Sbjct: 18  QLLDGLNPQQRQAVVHEGS-PLLIVAGAGSGKT 49



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>PCRA_MYCBO (P0A5A4) ATP-dependent DNA helicase pcrA (EC 3.6.1.-)|
          Length = 771

 Score = 31.6 bits (70), Expect = 2.0
 Identities = 17/33 (51%), Positives = 23/33 (69%)
 Frame = +2

Query: 536 EYLHSLNDKQREASCSDVAVPLMIVAGPGSGKT 634
           + L  LN +QR+A   + + PL+IVAG GSGKT
Sbjct: 18  QLLDGLNPQQRQAVVHEGS-PLLIVAGAGSGKT 49



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>PCRA_MYCLE (Q9CD72) ATP-dependent DNA helicase pcrA (EC 3.6.1.-)|
          Length = 778

 Score = 31.2 bits (69), Expect = 2.7
 Identities = 17/31 (54%), Positives = 22/31 (70%)
 Frame = +2

Query: 542 LHSLNDKQREASCSDVAVPLMIVAGPGSGKT 634
           L  LN +QR+A   + + PL+IVAG GSGKT
Sbjct: 18  LDGLNPQQRQAVVHEGS-PLLIVAGAGSGKT 47



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>NOTC2_HUMAN (Q04721) Neurogenic locus notch homolog protein 2 precursor (Notch 2)|
            (hN2) [Contains: Notch 2 extracellular truncation; Notch
            2 intracellular domain]
          Length = 2471

 Score = 31.2 bits (69), Expect = 2.7
 Identities = 15/43 (34%), Positives = 21/43 (48%), Gaps = 3/43 (6%)
 Frame = -3

Query: 581  YMKPPSACHLGSANTHSTSLGHTLHFP---PFLVPSPQTPCPW 462
            Y  PPS     S+N    +  H+ H     P+L PSP++P  W
Sbjct: 2383 YPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQW 2425



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>NOTC1_RAT (Q07008) Neurogenic locus notch homolog protein 1 precursor (Notch 1)|
            [Contains: Notch 1 extracellular truncation; Notch 1
            intracellular domain]
          Length = 2531

 Score = 30.8 bits (68), Expect = 3.5
 Identities = 16/42 (38%), Positives = 23/42 (54%), Gaps = 2/42 (4%)
 Frame = -3

Query: 581  YMKPPSACHLGSANTHSTSLGHTLHFP--PFLVPSPQTPCPW 462
            ++ PPS  H  S++    +  H L  P  PFL PSP++P  W
Sbjct: 2456 FLTPPSQ-HSYSSSPVDNTPSHQLQVPEHPFLTPSPESPDQW 2496



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>GPMA2_LACPL (Q88T35) 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase|
           2 (EC 5.4.2.1) (Phosphoglyceromutase 2) (PGAM 2)
           (BPG-dependent PGAM 2) (dPGM 2)
          Length = 230

 Score = 30.8 bits (68), Expect = 3.5
 Identities = 18/59 (30%), Positives = 29/59 (49%), Gaps = 4/59 (6%)
 Frame = +2

Query: 284 MNQEHYATSSAINQDEIEEVYVEDGSVAL----YVDFPPVISIAEEGGEVVDAFLGEVD 448
           +N+ HY     +N+ E  E Y +D  V +    Y   PP++S  +EG  V D    ++D
Sbjct: 85  LNERHYGALQGLNKKETAEKYGDD-QVHIWRRSYDVLPPLLSADDEGSAVNDRRYADLD 142



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>UBR1_YEAST (P19812) Ubiquitin-protein ligase E3 component N-recognin-1 (EC|
            6.-.-.-) (N-end-recognizing protein)
          Length = 1950

 Score = 30.4 bits (67), Expect = 4.6
 Identities = 27/115 (23%), Positives = 46/115 (40%), Gaps = 2/115 (1%)
 Frame = +2

Query: 233  FWEVNAISKEHHAISSEMNQEHYATSSAINQDEIEEVYVEDGSVALYVDFPPVISIAEEG 412
            FW  N +S  H A   + N E  + S  I+ +++  ++  D       D P +I    + 
Sbjct: 809  FWVRNGMSVLHQASYYKNNPELGSYSRDIHLNQLAILWERD-------DIPRIIYNILDR 861

Query: 413  GEVVDAFLGEVDAMSTA--XXXXXXXXXXXXXXXXXCAPRKYYEYLHSLNDKQRE 571
             E++D F GEVD   T                       R+Y++   SL D++ +
Sbjct: 862  WELLDWFTGEVDYQHTVYEDKISFIIQQFIAFIYQILTERQYFKTFSSLKDRRMD 916



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>UVRD_RICPR (Q9ZD95) Probable DNA helicase II homolog (EC 3.6.1.-)|
          Length = 658

 Score = 30.4 bits (67), Expect = 4.6
 Identities = 13/32 (40%), Positives = 24/32 (75%)
 Frame = +2

Query: 539 YLHSLNDKQREASCSDVAVPLMIVAGPGSGKT 634
           ++H+LN +Q++A+      PL+++AG G+GKT
Sbjct: 10  FMHTLNAEQKKAALHTEG-PLLLLAGAGTGKT 40



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>YHU3_YEAST (P38844) Protein YHR143W precursor|
          Length = 325

 Score = 30.0 bits (66), Expect = 6.0
 Identities = 35/133 (26%), Positives = 50/133 (37%)
 Frame = -2

Query: 441 SPKNASTTSPPSSAMEITGGKSTYNATEPXXXXXXXXXSWLIAEDVA*CSWFISEDMAWC 262
           S    ST SP  S+   T   STY+  E          S   AE  A   + ++   A+ 
Sbjct: 103 STSTVSTISPTISSGSSTSSSSTYDI-ESSQSIESSGTSSATAEPSASSGFRLTSSSAFV 161

Query: 261 SLLMALTSQKVSSNLTLSSAMDIAGRSESSPQDSTKLXXXXXXXXXXSWRLAAG*ASHSS 82
           S     ++   SS L+ SS+ + +  S S+   S  L          S+      A  SS
Sbjct: 162 S-----STAPFSSQLSSSSSSETSSSSFSTSSSSAPLSLTSSSSSSSSFATIITLAPSSS 216

Query: 81  KMSYASFQNASSS 43
           K   +    ASSS
Sbjct: 217 KSGNSQLTLASSS 229



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>NOTC2_RAT (Q9QW30) Neurogenic locus notch homolog protein 2 precursor (Notch 2)|
            [Contains: Notch 2 extracellular truncation; Notch 2
            intracellular domain]
          Length = 2471

 Score = 30.0 bits (66), Expect = 6.0
 Identities = 15/43 (34%), Positives = 20/43 (46%), Gaps = 3/43 (6%)
 Frame = -3

Query: 581  YMKPPSACHLGSANTHSTSLGHTLHFP---PFLVPSPQTPCPW 462
            Y  PPS     S+N    +  H  H     P+L PSP++P  W
Sbjct: 2383 YPTPPSQHSYASSNAAERTPNHGGHLQGEHPYLTPSPESPDQW 2425



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>NOTC2_MOUSE (O35516) Neurogenic locus notch homolog protein 2 precursor (Notch 2)|
            (Motch B) [Contains: Notch 2 extracellular truncation;
            Notch 2 intracellular domain]
          Length = 2470

 Score = 30.0 bits (66), Expect = 6.0
 Identities = 15/43 (34%), Positives = 20/43 (46%), Gaps = 3/43 (6%)
 Frame = -3

Query: 581  YMKPPSACHLGSANTHSTSLGHTLHFP---PFLVPSPQTPCPW 462
            Y  PPS     S+N    +  H  H     P+L PSP++P  W
Sbjct: 2382 YPTPPSQHSYASSNAAERTPSHGGHLQGEHPYLTPSPESPDQW 2424



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>NOTCH_XENLA (P21783) Neurogenic locus notch protein homolog precursor (XOTCH|
            protein)
          Length = 2524

 Score = 29.6 bits (65), Expect = 7.8
 Identities = 19/55 (34%), Positives = 28/55 (50%), Gaps = 4/55 (7%)
 Frame = -3

Query: 614  LQRSLTEQLH--HYMKPPSACHLGSANTHSTSLGHTLHFP--PFLVPSPQTPCPW 462
            L  +LT+ +    ++ PPS     S   ++ S  H L  P  PFL PSP++P  W
Sbjct: 2437 LPSNLTQSMTTAQFLTPPSQHSYSSPMDNTPS--HQLQVPDHPFLTPSPESPDQW 2489



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>AGA1_YEAST (P32323) A-agglutinin attachment subunit precursor|
          Length = 725

 Score = 29.6 bits (65), Expect = 7.8
 Identities = 35/137 (25%), Positives = 52/137 (37%)
 Frame = -2

Query: 453 MASTSPKNASTTSPPSSAMEITGGKSTYNATEPXXXXXXXXXSWLIAEDVA*CSWFISED 274
           ++ST+  N +TTS  S++   T   ST  +T P         +   +   +  S   S  
Sbjct: 173 LSSTTSSNPTTTSLSSTS---TSPSST--STSPSSTSTSSSSTSTSSSSTSTSSSSTSTS 227

Query: 273 MAWCSLLMALTSQKVSSNLTLSSAMDIAGRSESSPQDSTKLXXXXXXXXXXSWRLAAG*A 94
            +  S   +LTS   SS  T  S+   +  S S+   ST            S   +A   
Sbjct: 228 PSSTSTSSSLTSTSSSSTSTSQSSTSTSSSSTSTSPSSTSTSSSSTSTSPSSKSTSASST 287

Query: 93  SHSSKMSYASFQNASSS 43
           S SS  +  S    SSS
Sbjct: 288 STSSYSTSTSPSLTSSS 304



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>YLU2_PICAN (P34735) Hypothetical protein in LEU2 3'region (Fragment)|
          Length = 373

 Score = 29.6 bits (65), Expect = 7.8
 Identities = 36/138 (26%), Positives = 55/138 (39%)
 Frame = -2

Query: 456 DMASTSPKNASTTSPPSSAMEITGGKSTYNATEPXXXXXXXXXSWLIAEDVA*CSWFISE 277
           D+ S +P  + TTS     ME  GG  +Y+                  +  +  S   S 
Sbjct: 67  DVPSDNPDGSCTTSCTGYPMEKCGGSDSYSVYVDESEENDD-------DSSSAQSSHSST 119

Query: 276 DMAWCSLLMALTSQKVSSNLTLSSAMDIAGRSESSPQDSTKLXXXXXXXXXXSWRLAAG* 97
           D A  +   + TS   SS+   SS+   + +  SSPQ ST               L+A  
Sbjct: 120 DDATSTSSTSTTSS--SSSSLSSSSTSSSSKQSSSPQSSTMSSTDSSPTSSS---LSASS 174

Query: 96  ASHSSKMSYASFQNASSS 43
            + SS  S++  Q++SSS
Sbjct: 175 TTTSSISSFSFSQSSSSS 192


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,972,918
Number of Sequences: 219361
Number of extensions: 1570114
Number of successful extensions: 5107
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 4890
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5102
length of database: 80,573,946
effective HSP length: 107
effective length of database: 57,102,319
effective search space used: 5881538857
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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