ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name baalf14
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1HKT6_ORYSA (Q6H501) Probable cation transporter HKT6 (OsHKT6) 188 9e-48
2HKT4_ORYSA (Q7XPF8) Cation transporter HKT4 (OsHKT4) 103 3e-22
3HKT8_ORYSA (Q3YAF4) Cation transporter HKT8 (OsHKT8) 75 1e-13
4HKT9_ORYSA (Q8L4K5) Probable cation transporter HKT9 (OsHKT9) 71 2e-12
5HKT3_ORYSA (Q8L481) Probable cation transporter HKT3 (OsHKT3) 65 1e-10
6HKT2_ORYSA (Q93XI5) Cation transporter HKT2 (OsHKT2) (Po-OsHKT2) 61 2e-09
7HKT1_ORYSA (Q6TPH3) Cation transporter HKT1 (OsHKT1) (Ni-OsHKT1)... 60 4e-09
8HKT7_ORYSA (Q7XPF7) Probable cation transporter HKT7 (OsHKT7) 60 5e-09
9HKT1_ARATH (Q84TI7) Sodium transporter HKT1 (AtHKT1) 53 6e-07
10RK35_SPIOL (P23326) 50S ribosomal protein L35, chloroplast precu... 30 5.5
11VPH1_YEAST (P32563) Vacuolar ATP synthase subunit a, vacuolar is... 29 9.3
12RPOC1_CYACA (O19897) DNA-directed RNA polymerase beta' chain (EC... 29 9.3

>HKT6_ORYSA (Q6H501) Probable cation transporter HKT6 (OsHKT6)|
          Length = 530

 Score =  188 bits (477), Expect = 9e-48
 Identities = 101/176 (57%), Positives = 119/176 (67%)
 Frame = +1

Query: 1   MNNSPVVHLKSLRTFXAFVATKLSYFAKSAQQSMKYSCQFICHTNPLFIQVTYFALISFA 180
           MN+  VV  K L+TF  F A+K S F KSAQ+S+KYS QFI   NPLF+ V YFALISFA
Sbjct: 1   MNHCLVVSHKKLQTFRTFAASKFSSFTKSAQKSIKYSFQFIYQNNPLFVHVAYFALISFA 60

Query: 181 GYEALKVLNSQDKSNTLKDLXVLFXXXXXXXXXXXXXXEMEEFSSKQLWILAILMLIGXE 360
           GY +LKVL  +DKSNTLKDL VLF              EME+FSS QLW+L ILMLIG E
Sbjct: 61  GYGSLKVLKPRDKSNTLKDLDVLFTSVSASTVSSMATVEMEDFSSAQLWVLTILMLIGGE 120

Query: 361 VFTSILGLHFXRAKFNSXNSFNRRDQISHVDIESINVTNFDPTASHGTKDGASFSE 528
           VFTS+LG+HF RA+F +  S + RD    +DIESI  T F P ++ GTK   SFSE
Sbjct: 121 VFTSMLGIHFMRAEFGTKESVSTRDHSPCIDIESITSTKFGP-STQGTKVTVSFSE 175



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>HKT4_ORYSA (Q7XPF8) Cation transporter HKT4 (OsHKT4)|
          Length = 552

 Score =  103 bits (257), Expect = 3e-22
 Identities = 59/150 (39%), Positives = 83/150 (55%), Gaps = 1/150 (0%)
 Frame = +1

Query: 13  PVVHLKSLRTFXAFVATKLSYFAKSAQQSMKYSCQF-ICHTNPLFIQVTYFALISFAGYE 189
           P ++ +  +    F++ KL   A+    S+K S  F +C +NPL +Q+ YF +ISFAG+ 
Sbjct: 30  PRIYWEKAQHLCGFLSMKLISRARCVASSVKQSYSFLVCKSNPLVVQLVYFVIISFAGFL 89

Query: 190 ALKVLNSQDKSNTLKDLXVLFXXXXXXXXXXXXXXEMEEFSSKQLWILAILMLIGXEVFT 369
           ALK L  Q K    KDL +LF              EME+ S +QLW+L +LML+G EVFT
Sbjct: 90  ALKNLKPQGKPGP-KDLDLLFTSVSTLTVSSMATVEMEDLSDRQLWVLILLMLMGGEVFT 148

Query: 370 SILGLHFXRAKFNSXNSFNRRDQISHVDIE 459
           S+LGL+F  A  N   +  R      +DIE
Sbjct: 149 SMLGLYFNNANANRNENSQRSLPSISLDIE 178



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>HKT8_ORYSA (Q3YAF4) Cation transporter HKT8 (OsHKT8)|
          Length = 554

 Score = 75.1 bits (183), Expect = 1e-13
 Identities = 39/99 (39%), Positives = 56/99 (56%), Gaps = 1/99 (1%)
 Frame = +1

Query: 118 FICHTNPLFIQVTYFALISFAGYEALKVLNSQDKSNTLK-DLXVLFXXXXXXXXXXXXXX 294
           F+ H +P ++Q+ YF  IS  G+  LK L  +        DL ++F              
Sbjct: 21  FLFHAHPFWLQLLYFLFISLLGFLMLKALPMKTSMVPRPMDLDLIFTSVSATTVSSMVAV 80

Query: 295 EMEEFSSKQLWILAILMLIGXEVFTSILGLHFXRAKFNS 411
           EME FS+ QL ++ +LML+G EVFTSILGL+F  AK++S
Sbjct: 81  EMESFSNSQLLLITLLMLLGGEVFTSILGLYFTNAKYSS 119



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>HKT9_ORYSA (Q8L4K5) Probable cation transporter HKT9 (OsHKT9)|
          Length = 509

 Score = 70.9 bits (172), Expect = 2e-12
 Identities = 41/127 (32%), Positives = 64/127 (50%), Gaps = 4/127 (3%)
 Frame = +1

Query: 64  KLSYFAKSAQQSMKYS---CQFIC-HTNPLFIQVTYFALISFAGYEALKVLNSQDKSNTL 231
           +L  F  SA+ ++  S   C+FI  H +PL I ++YF +I   G+ AL VL   +     
Sbjct: 4   RLHIFVSSARHAINSSALICRFIAFHLSPLLIHLSYFLIIDVLGFVALVVLRPSNHKYNP 63

Query: 232 KDLXVLFXXXXXXXXXXXXXXEMEEFSSKQLWILAILMLIGXEVFTSILGLHFXRAKFNS 411
           + + + F              +ME+ SS Q+ +L +LM +G E+F S LGL    +K N 
Sbjct: 64  RYIDMFFLSTSAVTVTGLATTQMEDLSSSQIAVLTLLMFLGSEMFLSFLGLVLESSKQNK 123

Query: 412 XNSFNRR 432
            +  NRR
Sbjct: 124 HDPENRR 130



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>HKT3_ORYSA (Q8L481) Probable cation transporter HKT3 (OsHKT3)|
          Length = 509

 Score = 65.1 bits (157), Expect = 1e-10
 Identities = 37/119 (31%), Positives = 59/119 (49%), Gaps = 1/119 (0%)
 Frame = +1

Query: 79  AKSAQQSMKYSCQFIC-HTNPLFIQVTYFALISFAGYEALKVLNSQDKSNTLKDLXVLFX 255
           A+ A  S  + C+FI  H +PL I ++YF +I   G+ +L VL   +     + + + F 
Sbjct: 12  ARHAINSSAFICRFIAYHLSPLLIHLSYFLIIDILGFVSLVVLRPSNHKYNPRYVDMFFL 71

Query: 256 XXXXXXXXXXXXXEMEEFSSKQLWILAILMLIGXEVFTSILGLHFXRAKFNSXNSFNRR 432
                        +ME+ SS Q+ IL +LM +  ++F S LGL    +K N  +  NRR
Sbjct: 72  STSAVTVIGLATIQMEDLSSSQIAILTLLMFLDSKMFLSFLGLVLESSKQNKHDPENRR 130



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>HKT2_ORYSA (Q93XI5) Cation transporter HKT2 (OsHKT2) (Po-OsHKT2)|
          Length = 530

 Score = 61.2 bits (147), Expect = 2e-09
 Identities = 40/111 (36%), Positives = 53/111 (47%)
 Frame = +1

Query: 127 HTNPLFIQVTYFALISFAGYEALKVLNSQDKSNTLKDLXVLFXXXXXXXXXXXXXXEMEE 306
           H +P +IQ++YF LIS  G   L  L           + +LF              EME 
Sbjct: 37  HVHPFWIQLSYFLLISILGSVLLMFLKPSSPEFKPGYIDMLFLSTSAMTVSGLSTIEMEV 96

Query: 307 FSSKQLWILAILMLIGXEVFTSILGLHFXRAKFNSXNSFNRRDQISHVDIE 459
            SS Q+ +L +LML+G EVF S LGL   R K      F+  D++S V IE
Sbjct: 97  LSSSQIVVLTLLMLVGGEVFVSFLGL-MLRLKHKHNPEFS-GDRVSSVPIE 145



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>HKT1_ORYSA (Q6TPH3) Cation transporter HKT1 (OsHKT1) (Ni-OsHKT1) (Po-OsHKT1)|
          Length = 530

 Score = 60.1 bits (144), Expect = 4e-09
 Identities = 44/140 (31%), Positives = 66/140 (47%), Gaps = 4/140 (2%)
 Frame = +1

Query: 52  FVATKLSYFAKSAQQSMKY---SCQFIC-HTNPLFIQVTYFALISFAGYEALKVLNSQDK 219
           F+  KL  F +  +  + +   + +FI  H +P +IQ++YF LIS  G   L  L   + 
Sbjct: 8   FIHNKLQSFGRIGRYFVNFVVLAHRFIALHIHPFWIQLSYFLLISILGSVLLMFLKPSNP 67

Query: 220 SNTLKDLXVLFXXXXXXXXXXXXXXEMEEFSSKQLWILAILMLIGXEVFTSILGLHFXRA 399
                 + +LF              EME  SS Q+ ++ +LML+G EVF S LGL   R 
Sbjct: 68  EFRPGYIDMLFLSTSALTLSSLITIEMEVLSSSQIVVITLLMLLGGEVFVSFLGL-MLRL 126

Query: 400 KFNSXNSFNRRDQISHVDIE 459
                  F+  D++S V IE
Sbjct: 127 NHKHNPEFS-GDKVSSVPIE 145



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>HKT7_ORYSA (Q7XPF7) Probable cation transporter HKT7 (OsHKT7)|
          Length = 500

 Score = 59.7 bits (143), Expect = 5e-09
 Identities = 40/122 (32%), Positives = 56/122 (45%), Gaps = 3/122 (2%)
 Frame = +1

Query: 139 LFIQVTYFALISFAGYEALKVLNSQDKSNTLKDLXVLFXXXXXXXXXXXXXXEMEEFSSK 318
           L + V YF  IS  GY  L VL  ++     + +   F              EME FS+ 
Sbjct: 13  LSMHVAYFLAISCLGYGLLGVLKVREPGAAPRRIDRFFTAVSAATVSSMSTVEMEVFSNG 72

Query: 319 QLWILAILMLIGXEVFTSILGLHFXRAKFNSXNSFNRRDQISHVDIESINVTNFD---PT 489
           QL +L +LML+G EVF S++GL    +K  S      R   SH D+   ++   D   PT
Sbjct: 73  QLVVLTVLMLLGGEVFVSLVGLASKWSKLRSDAMDRSRRVESHGDVALADIDGGDVENPT 132

Query: 490 AS 495
           +S
Sbjct: 133 SS 134



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>HKT1_ARATH (Q84TI7) Sodium transporter HKT1 (AtHKT1)|
          Length = 506

 Score = 52.8 bits (125), Expect = 6e-07
 Identities = 29/82 (35%), Positives = 39/82 (47%)
 Frame = +1

Query: 142 FIQVTYFALISFAGYEALKVLNSQDKSNTLKDLXVLFXXXXXXXXXXXXXXEMEEFSSKQ 321
           F+   YF   SF G+ ALK+   +  S    D  + F              +ME FS+ Q
Sbjct: 23  FLYFIYFLFFSFLGFLALKITKPRTTSRP-HDFDLFFTSVSAITVSSMSTVDMEVFSNTQ 81

Query: 322 LWILAILMLIGXEVFTSILGLH 387
           L  L ILM +G E+FTS L L+
Sbjct: 82  LIFLTILMFLGGEIFTSFLNLY 103



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>RK35_SPIOL (P23326) 50S ribosomal protein L35, chloroplast precursor (CL35)|
          Length = 159

 Score = 29.6 bits (65), Expect = 5.5
 Identities = 20/62 (32%), Positives = 34/62 (54%), Gaps = 1/62 (1%)
 Frame = -3

Query: 368 VNTSLPISIKIAKIQSCLLENSSISTVAMLETVDAETEVNNTXKSFRVFDL-SWELRTFR 192
           +N S   SI  +K+Q  +L+N  ISTV        ++ V+ +  SF VF    ++++T +
Sbjct: 45  LNLSSSSSISSSKVQPIVLKNKRISTV--------DSSVSTSSPSFTVFAAKGYKMKTHK 96

Query: 191 AS 186
           AS
Sbjct: 97  AS 98



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>VPH1_YEAST (P32563) Vacuolar ATP synthase subunit a, vacuolar isoform (EC|
           3.6.3.14) (V-ATPase a subunit) (Vacuolar proton pump a
           subunit) (V-ATPase 95 kDa subunit) (Vacuolar pH protein
           1)
          Length = 839

 Score = 28.9 bits (63), Expect = 9.3
 Identities = 22/100 (22%), Positives = 47/100 (47%), Gaps = 12/100 (12%)
 Frame = -3

Query: 371 EVNTSLPISIKIAKIQSCL---LENSSISTVAMLETVDAETEVNNTXKSFRVFDLSWELR 201
           E+N  LP  +    + + +   + +  + T+A L  V  E ++N   +   +FD+++  R
Sbjct: 403 EINAGLPTIVTFPFMFAIMFGDMGHGFLMTLAALSLVLNEKKINKMKRG-EIFDMAFTGR 461

Query: 200 ---------TFRASYPANEIKAK*VTWIKSGFVWQMNWQE 108
                    +    +  N+I +K +T  KSG+ W  +W++
Sbjct: 462 YIILLMGVFSMYTGFLYNDIFSKTMTIFKSGWKWPDHWKK 501



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>RPOC1_CYACA (O19897) DNA-directed RNA polymerase beta' chain (EC 2.7.7.6) (PEP)|
           (Plastid-encoded RNA polymerase beta' subunit) (RNA
           polymerase beta' subunit)
          Length = 633

 Score = 28.9 bits (63), Expect = 9.3
 Identities = 14/56 (25%), Positives = 30/56 (53%)
 Frame = +1

Query: 7   NSPVVHLKSLRTFXAFVATKLSYFAKSAQQSMKYSCQFICHTNPLFIQVTYFALIS 174
           ++PVVH+  L+   ++++  L   AK  +Q + ++   I H+     Q+ Y  L++
Sbjct: 110 SAPVVHIWYLKGMPSYISVFLDMAAKEIEQVVYFNTYIITHSTAENKQIKYKQLLT 165


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 67,007,308
Number of Sequences: 219361
Number of extensions: 1174691
Number of successful extensions: 2546
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 2497
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2544
length of database: 80,573,946
effective HSP length: 105
effective length of database: 57,541,041
effective search space used: 4142954952
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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