ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name baak4c20
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1CYT2_ORYSA (P20907) Cysteine proteinase inhibitor 2 (Oryzacystat... 125 3e-30
2CYT1_ORYSA (P09229) Cysteine proteinase inhibitor 1 (Oryzacystat... 120 1e-27
3CYT1_MAIZE (P31726) Cystatin-1 precursor (Cystatin I) (Corn kern... 117 9e-27
4CYTA_HELAN (Q10992) Cysteine proteinase inhibitor A (Cystatin A)... 105 6e-23
5CYTI_VIGUN (Q06445) Cysteine proteinase inhibitor (Cystatin) 100 1e-21
6CYTM_SOLTU (P37842) Multicystatin (MC) 83 3e-16
7CYT_SOLTU (Q03196) Cysteine proteinase inhibitor (Fragment) 71 9e-13
8CYTB_HELAN (Q10993) Cysteine proteinase inhibitor B (Cystatin B)... 47 2e-05
9CYT_SOYBN (P25973) Cystatin (Low molecular mass protein inhibito... 42 5e-04
10CYT_ONCKE (Q98967) Cystatin precursor 37 0.020
11CYT_ONCMY (Q91195) Cystatin precursor 37 0.026
12CYT_CYPCA (P35481) Cystatin precursor (Ovarian cystatin) (P12) 37 0.026
13CYTX_ONCVO (P22085) Onchocystatin precursor (Cysteine proteinase... 35 0.058
14CYTL_TACTR (Q7M429) L-cystatin precursor 34 0.13
15CYTL_DROME (P23779) Cystatin-like protein 33 0.37
16TRA_CORDI (P35879) Probable transposase for insertion sequence e... 31 1.1
17CRLF2_RAT (Q8R4S8) Cytokine receptor-like factor 2 precursor (Th... 30 1.9
18CYTN_HUMAN (P01037) Cystatin SN precursor (Salivary cystatin SA-... 29 4.1

>CYT2_ORYSA (P20907) Cysteine proteinase inhibitor 2 (Oryzacystatin-2)|
           (Oryzacystatin II)
          Length = 108

 Score =  125 bits (314), Expect(2) = 3e-30
 Identities = 60/73 (82%), Positives = 67/73 (91%), Gaps = 1/73 (1%)
 Frame = +3

Query: 39  IELARFAVAEHNAKANALLEFERLVKVRQQVVAGCMHYFTIEVKE-GGAKKLYEAKVWEK 215
           +ELARFAVAEHN+KANA+LE ER+VKVRQQVV G MHY T+EVKE GGA KLYEAKVWE+
Sbjct: 30  VELARFAVAEHNSKANAMLELERVVKVRQQVVGGFMHYLTVEVKEPGGANKLYEAKVWER 89

Query: 216 AWENFKQLQEFKP 254
           AWENFKQLQ+FKP
Sbjct: 90  AWENFKQLQDFKP 102



 Score = 25.0 bits (53), Expect(2) = 3e-30
 Identities = 10/10 (100%), Positives = 10/10 (100%)
 Frame = +2

Query: 2  DAPAGRENDL 31
          DAPAGRENDL
Sbjct: 18 DAPAGRENDL 27



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>CYT1_ORYSA (P09229) Cysteine proteinase inhibitor 1 (Oryzacystatin-1)|
           (Oryzacystatin I)
          Length = 102

 Score =  120 bits (301), Expect = 1e-27
 Identities = 56/72 (77%), Positives = 65/72 (90%)
 Frame = +3

Query: 39  IELARFAVAEHNAKANALLEFERLVKVRQQVVAGCMHYFTIEVKEGGAKKLYEAKVWEKA 218
           ++LARFAV EHN KAN+LLEFE+LV V+QQVVAG ++YFTIEVKEG AKKLYEAKVWEK 
Sbjct: 24  VDLARFAVTEHNKKANSLLEFEKLVSVKQQVVAGTLYYFTIEVKEGDAKKLYEAKVWEKP 83

Query: 219 WENFKQLQEFKP 254
           W +FK+LQEFKP
Sbjct: 84  WMDFKELQEFKP 95



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>CYT1_MAIZE (P31726) Cystatin-1 precursor (Cystatin I) (Corn kernel cysteine|
           proteinase inhibitor)
          Length = 135

 Score =  117 bits (294), Expect = 9e-27
 Identities = 57/71 (80%), Positives = 61/71 (85%)
 Frame = +3

Query: 42  ELARFAVAEHNAKANALLEFERLVKVRQQVVAGCMHYFTIEVKEGGAKKLYEAKVWEKAW 221
           ELARFAV EHN KANALL FE+LVK + QVVAG M+Y TIEVK+G  KKLYEAKVWEK W
Sbjct: 58  ELARFAVNEHNQKANALLGFEKLVKAKTQVVAGTMYYLTIEVKDGEVKKLYEAKVWEKPW 117

Query: 222 ENFKQLQEFKP 254
           ENFKQLQEFKP
Sbjct: 118 ENFKQLQEFKP 128



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>CYTA_HELAN (Q10992) Cysteine proteinase inhibitor A (Cystatin A) (SCA)|
          Length = 82

 Score =  105 bits (261), Expect = 6e-23
 Identities = 49/71 (69%), Positives = 56/71 (78%)
 Frame = +3

Query: 42  ELARFAVAEHNAKANALLEFERLVKVRQQVVAGCMHYFTIEVKEGGAKKLYEAKVWEKAW 221
           ELARFAV EHN K NALLEF ++V  ++QVVAG M+Y T+E   GG KK YEAKVW K W
Sbjct: 6   ELARFAVDEHNKKQNALLEFGKVVNTKEQVVAGKMYYITLEATNGGVKKTYEAKVWVKPW 65

Query: 222 ENFKQLQEFKP 254
           ENFK+LQEFKP
Sbjct: 66  ENFKELQEFKP 76



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>CYTI_VIGUN (Q06445) Cysteine proteinase inhibitor (Cystatin)|
          Length = 97

 Score =  100 bits (250), Expect = 1e-21
 Identities = 47/69 (68%), Positives = 56/69 (81%)
 Frame = +3

Query: 45  LARFAVAEHNAKANALLEFERLVKVRQQVVAGCMHYFTIEVKEGGAKKLYEAKVWEKAWE 224
           LARFAV EHN K NALLEF R+V  +QQVV+G ++  T+E K+GG KK+YEAKVWEK W 
Sbjct: 22  LARFAVEEHNKKQNALLEFGRVVSAQQQVVSGTLYTITLEAKDGGQKKVYEAKVWEKPWL 81

Query: 225 NFKQLQEFK 251
           NFK+LQEFK
Sbjct: 82  NFKELQEFK 90



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>CYTM_SOLTU (P37842) Multicystatin (MC)|
          Length = 756

 Score = 82.8 bits (203), Expect = 3e-16
 Identities = 37/70 (52%), Positives = 52/70 (74%)
 Frame = +3

Query: 42  ELARFAVAEHNAKANALLEFERLVKVRQQVVAGCMHYFTIEVKEGGAKKLYEAKVWEKAW 221
           +LARFAV ++N K NA LEF   + V++Q+VAG ++Y T+   + G KK+YEAK+W K W
Sbjct: 492 DLARFAVQDYNKKENAHLEFVENLNVKEQLVAGMLYYITLVAIDAGKKKIYEAKIWVKEW 551

Query: 222 ENFKQLQEFK 251
           ENFK++ EFK
Sbjct: 552 ENFKKVIEFK 561



 Score = 81.3 bits (199), Expect = 9e-16
 Identities = 36/70 (51%), Positives = 49/70 (70%)
 Frame = +3

Query: 42  ELARFAVAEHNAKANALLEFERLVKVRQQVVAGCMHYFTIEVKEGGAKKLYEAKVWEKAW 221
           +L RFAV ++N   NA LEF   + V++QVVAG ++Y T    +GG KK+YE K+W K W
Sbjct: 398 DLTRFAVHQYNKDQNAHLEFVENLNVKKQVVAGMLYYITFAATDGGKKKIYETKIWVKVW 457

Query: 222 ENFKQLQEFK 251
           ENFK++ EFK
Sbjct: 458 ENFKKVVEFK 467



 Score = 80.5 bits (197), Expect = 2e-15
 Identities = 35/70 (50%), Positives = 52/70 (74%)
 Frame = +3

Query: 42  ELARFAVAEHNAKANALLEFERLVKVRQQVVAGCMHYFTIEVKEGGAKKLYEAKVWEKAW 221
           +LARFAV ++N K NA LE+   + V++Q+VAG ++Y T+   + G KK+YEAK+W K W
Sbjct: 586 DLARFAVQDYNKKENAHLEYVENLNVKEQLVAGMIYYITLVATDAGKKKIYEAKIWVKEW 645

Query: 222 ENFKQLQEFK 251
           E+FK++ EFK
Sbjct: 646 EDFKKVVEFK 655



 Score = 80.1 bits (196), Expect = 2e-15
 Identities = 36/70 (51%), Positives = 49/70 (70%)
 Frame = +3

Query: 42  ELARFAVAEHNAKANALLEFERLVKVRQQVVAGCMHYFTIEVKEGGAKKLYEAKVWEKAW 221
           +LARFA+  +N K N  LEF   + V+QQVVAG M+Y T+   + G KK+YE K+W K W
Sbjct: 209 DLARFAIQVYNKKENVHLEFVENLNVKQQVVAGMMYYITLAAIDAGKKKIYETKIWVKEW 268

Query: 222 ENFKQLQEFK 251
           E+FK++ EFK
Sbjct: 269 EDFKKVVEFK 278



 Score = 79.3 bits (194), Expect = 3e-15
 Identities = 37/71 (52%), Positives = 52/71 (73%), Gaps = 1/71 (1%)
 Frame = +3

Query: 42  ELARFAVAEHNAKANALLEFERLVKVRQQVVAGCMHYFTI-EVKEGGAKKLYEAKVWEKA 218
           ELARFA+ ++N K NA LEF   + V++QVVAG M+Y T+    + G KK+Y+AK+W K 
Sbjct: 114 ELARFAIQDYNKKQNAHLEFVENLNVKEQVVAGIMYYITLAATDDAGKKKIYKAKIWVKE 173

Query: 219 WENFKQLQEFK 251
           WE+FK++ EFK
Sbjct: 174 WEDFKKVVEFK 184



 Score = 77.0 bits (188), Expect = 2e-14
 Identities = 35/70 (50%), Positives = 52/70 (74%)
 Frame = +3

Query: 42  ELARFAVAEHNAKANALLEFERLVKVRQQVVAGCMHYFTIEVKEGGAKKLYEAKVWEKAW 221
           +LARFAV ++N K ++ LEF++++ V+QQ+VAG M+Y T E  EGG KK YEAK+  + W
Sbjct: 20  DLARFAVQDYNQKNDSSLEFKKVLNVKQQIVAGIMYYITFEATEGGNKKEYEAKILLRKW 79

Query: 222 ENFKQLQEFK 251
           E+ K++  FK
Sbjct: 80  EDLKKVVGFK 89



 Score = 75.9 bits (185), Expect = 4e-14
 Identities = 35/70 (50%), Positives = 48/70 (68%)
 Frame = +3

Query: 42  ELARFAVAEHNAKANALLEFERLVKVRQQVVAGCMHYFTIEVKEGGAKKLYEAKVWEKAW 221
           +LARFAV + N K N  LEF   + V++QVVAG M+Y T+   +   K++YE K+  K W
Sbjct: 680 DLARFAVQDFNKKENGHLEFVENLNVKEQVVAGMMYYITLAATDARKKEIYETKILVKEW 739

Query: 222 ENFKQLQEFK 251
           ENFK++QEFK
Sbjct: 740 ENFKEVQEFK 749



 Score = 75.9 bits (185), Expect = 4e-14
 Identities = 34/70 (48%), Positives = 50/70 (71%)
 Frame = +3

Query: 42  ELARFAVAEHNAKANALLEFERLVKVRQQVVAGCMHYFTIEVKEGGAKKLYEAKVWEKAW 221
           +LARFAV ++N   NA LEF   + V++Q+V+G M+Y T+   + G KK YEAK+W K W
Sbjct: 303 DLARFAVQDYNNTQNAHLEFVENLNVKEQLVSGMMYYITLAATDAGNKKEYEAKIWVKEW 362

Query: 222 ENFKQLQEFK 251
           E+FK++ +FK
Sbjct: 363 EDFKKVIDFK 372



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>CYT_SOLTU (Q03196) Cysteine proteinase inhibitor (Fragment)|
          Length = 66

 Score = 71.2 bits (173), Expect = 9e-13
 Identities = 30/56 (53%), Positives = 42/56 (75%)
 Frame = +3

Query: 84  NALLEFERLVKVRQQVVAGCMHYFTIEVKEGGAKKLYEAKVWEKAWENFKQLQEFK 251
           NA LEF  ++ V++QVVAG M+Y T+   + G KK+Y+ K+W K WENFK++QEFK
Sbjct: 4   NAHLEFVEVLNVKEQVVAGMMYYITLVATDDGYKKIYKTKIWVKEWENFKEVQEFK 59



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>CYTB_HELAN (Q10993) Cysteine proteinase inhibitor B (Cystatin B) (SCB)|
          Length = 101

 Score = 47.0 bits (110), Expect = 2e-05
 Identities = 26/79 (32%), Positives = 46/79 (58%), Gaps = 7/79 (8%)
 Frame = +3

Query: 42  ELARFAVAEHNAKANAL------LEFERLVKVRQQVVAGCMHYFTIE-VKEGGAKKLYEA 200
           +L  ++V E+N            L+F +++    QVVAG  +Y  IE + +GG  K+++A
Sbjct: 19  QLGSYSVDEYNRLQRTKKTGAGDLKFSQVIAAETQVVAGTKYYLKIEAITKGGKMKVFDA 78

Query: 201 KVWEKAWENFKQLQEFKPA 257
           +V  ++W++ K+L  FKPA
Sbjct: 79  EVVVQSWKHSKKLLGFKPA 97



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>CYT_SOYBN (P25973) Cystatin (Low molecular mass protein inhibitor of cysteine|
           proteinases) (Fragment)
          Length = 48

 Score = 42.4 bits (98), Expect = 5e-04
 Identities = 20/32 (62%), Positives = 25/32 (78%)
 Frame = +3

Query: 45  LARFAVAEHNAKANALLEFERLVKVRQQVVAG 140
           LARFAV EHN K NA+LEF R+   ++QVV+G
Sbjct: 17  LARFAVDEHNKKENAVLEFVRVKSAKKQVVSG 48



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>CYT_ONCKE (Q98967) Cystatin precursor|
          Length = 130

 Score = 37.0 bits (84), Expect = 0.020
 Identities = 21/78 (26%), Positives = 39/78 (50%), Gaps = 21/78 (26%)
 Frame = +3

Query: 51  RFAVAEHNAKANALL--EFERLVKVRQQVVAGCMHYFTIEV-----KEGGAKKLYEA--- 200
           +FAV EHN K N +   +  ++V  ++QVV+G  + FT+++     ++GG +K+      
Sbjct: 40  QFAVVEHNKKTNDMFVRQVAKVVNAQKQVVSGMKYIFTVQMGRTPCRKGGVEKICSVHKD 99

Query: 201 -----------KVWEKAW 221
                      +VW + W
Sbjct: 100 PQMAVPYKCTFEVWSRPW 117



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>CYT_ONCMY (Q91195) Cystatin precursor|
          Length = 130

 Score = 36.6 bits (83), Expect = 0.026
 Identities = 21/78 (26%), Positives = 39/78 (50%), Gaps = 21/78 (26%)
 Frame = +3

Query: 51  RFAVAEHNAKANALL--EFERLVKVRQQVVAGCMHYFTIEV-----KEGGAKKLYEA--- 200
           +FAV EHN K N +   +  ++V  ++QVV+G  + FT+++     ++GG +K+      
Sbjct: 40  QFAVVEHNKKTNDMFVRQVAKVVNAQKQVVSGMKYIFTVQMGRTPCRKGGVEKVCSVHKD 99

Query: 201 -----------KVWEKAW 221
                      +VW + W
Sbjct: 100 PQMAVPYKCTFEVWSRPW 117



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>CYT_CYPCA (P35481) Cystatin precursor (Ovarian cystatin) (P12)|
          Length = 129

 Score = 36.6 bits (83), Expect = 0.026
 Identities = 24/86 (27%), Positives = 42/86 (48%), Gaps = 21/86 (24%)
 Frame = +3

Query: 51  RFAVAEHNAKANALL--EFERLVKVRQQVVAGCMHYFTIEV-----KEGGAKKL------ 191
           RFAV  +N ++N     +  +++KV+QQV AG  + FT+++     K+GG K +      
Sbjct: 40  RFAVDHYNGQSNDAFVRKVSKVIKVQQQVAAGMKYIFTVKMEVASCKKGGVKTMCAVPKN 99

Query: 192 --------YEAKVWEKAWENFKQLQE 245
                    +  VW + W N  ++ E
Sbjct: 100 PSIEQVIQCKITVWSQPWLNSLKVTE 125



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>CYTX_ONCVO (P22085) Onchocystatin precursor (Cysteine proteinase inhibitor|
           OV7)
          Length = 162

 Score = 35.4 bits (80), Expect = 0.058
 Identities = 20/66 (30%), Positives = 31/66 (46%), Gaps = 20/66 (30%)
 Frame = +3

Query: 105 RLVKVRQQVVAGCMHYFTIEVK--------------------EGGAKKLYEAKVWEKAWE 224
           +L+KV  QVVAG  +   ++V                     EG  +K+   +VWEK WE
Sbjct: 90  KLLKVSSQVVAGVKYKMDVQVARSQCKKSSNEKVDLTKCKKLEGHPEKVMTLEVWEKPWE 149

Query: 225 NFKQLQ 242
           NF +++
Sbjct: 150 NFMRVE 155



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>CYTL_TACTR (Q7M429) L-cystatin precursor|
          Length = 133

 Score = 34.3 bits (77), Expect = 0.13
 Identities = 25/82 (30%), Positives = 37/82 (45%), Gaps = 22/82 (26%)
 Frame = +3

Query: 42  ELARFAVAEHNAKANALLEFE--RLVKVRQQVVAGCMHYFTIE----------------- 164
           E ARFA    ++++N+L   +  ++ K R QVV+G  +   IE                 
Sbjct: 37  EAARFATEAQSSRSNSLYHHKLLKIHKARTQVVSGINYEVFIETGTTTCKKSEVPLEDLK 96

Query: 165 ---VKEGGAKKLYEAKVWEKAW 221
              V E G K L +A VW +AW
Sbjct: 97  RCAVPENGVKHLCQAIVWVQAW 118



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>CYTL_DROME (P23779) Cystatin-like protein|
          Length = 126

 Score = 32.7 bits (73), Expect = 0.37
 Identities = 13/38 (34%), Positives = 21/38 (55%)
 Frame = +3

Query: 108 LVKVRQQVVAGCMHYFTIEVKEGGAKKLYEAKVWEKAW 221
           +  V  QVVAG ++ + +E+  G  KK    K+W + W
Sbjct: 66  VTSVTGQVVAGSLNTYEVELDNGSDKKQCTVKIWTQPW 103



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>TRA_CORDI (P35879) Probable transposase for insertion sequence element|
          Length = 343

 Score = 31.2 bits (69), Expect = 1.1
 Identities = 14/33 (42%), Positives = 16/33 (48%)
 Frame = +3

Query: 162 EVKEGGAKKLYEAKVWEKAWENFKQLQEFKPAA 260
           E  E G K     KVW  AW+ F    +F PAA
Sbjct: 218 EASELGRKYPQSVKVWRDAWDRFVPFLQFPPAA 250



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>CRLF2_RAT (Q8R4S8) Cytokine receptor-like factor 2 precursor (Thymic stromal|
           lymphopoietin protein receptor) (TSLPR)
          Length = 360

 Score = 30.4 bits (67), Expect = 1.9
 Identities = 21/71 (29%), Positives = 27/71 (38%), Gaps = 18/71 (25%)
 Frame = +3

Query: 63  AEHNAKANALLEFERLVKVRQQVVAGCMHYFTIE------------------VKEGGAKK 188
           A H   AN  LEF    +   QV   C HYF ++                  ++EGG  K
Sbjct: 55  AHHGLSANLSLEF----RYGNQVPQPCPHYFLLDSVRAGCVLPMGKGLLEVVLREGGGAK 110

Query: 189 LYEAKVWEKAW 221
           L+  K    AW
Sbjct: 111 LFSRKKKASAW 121



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>CYTN_HUMAN (P01037) Cystatin SN precursor (Salivary cystatin SA-1) (Cystain|
           SA-I)
          Length = 141

 Score = 29.3 bits (64), Expect = 4.1
 Identities = 22/84 (26%), Positives = 37/84 (44%), Gaps = 22/84 (26%)
 Frame = +3

Query: 54  FAVAEHN--AKANALLEFERLVKVRQQVVAGCMHYFTIEV-------------------- 167
           FA++E+N   K +      R+++ RQQ V G  ++F +EV                    
Sbjct: 50  FAISEYNKATKDDYYRRPLRVLRARQQTVGGVNYFFDVEVGRTICTKSQPNLDTCAFHEQ 109

Query: 168 KEGGAKKLYEAKVWEKAWENFKQL 239
            E   K+L   +++E  WEN + L
Sbjct: 110 PELQKKQLCSFEIYEVPWENRRSL 133


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,862,601
Number of Sequences: 219361
Number of extensions: 560200
Number of successful extensions: 1278
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1253
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1274
length of database: 80,573,946
effective HSP length: 101
effective length of database: 58,418,485
effective search space used: 2395157885
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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