ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | rbasdl21 |
|---|---|
| Clone Library Name | barley_pub |
>VPE_CITSI (P49043) Vacuolar processing enzyme precursor (EC 3.4.22.-) (VPE)| Length = 494 Score = 172 bits (435), Expect = 1e-42 Identities = 83/137 (60%), Positives = 104/137 (75%), Gaps = 1/137 (0%) Frame = -3 Query: 636 NATFVQSSSSSRQLPGAR-VNQRDADLVHFWHKYRRSAEGSAEKVEARRRLVETMARRSR 460 N TFV +S P ++ VNQRDADL+HFW KYR++ EG+ K EA+++ E M+ R Sbjct: 336 NYTFVDENSLR---PASKAVNQRDADLLHFWDKYRKAPEGTPRKAEAQKQFFEAMSHRMH 392 Query: 459 VDSSVELIGGLLFGSEEGAKVLGTVRPAGQPVVDDWGCLKSVVRRFEERCGPLTQYGMKH 280 VD S++LIG LLFG E+G ++L TVRPAGQP+VDDWGCLKS+VR FE CG L+QYGMKH Sbjct: 393 VDHSIKLIGKLLFGIEKGPEILNTVRPAGQPLVDDWGCLKSLVRTFESHCGALSQYGMKH 452 Query: 279 MRSLANICNAGVREEVM 229 MRSLANICN G+ +E M Sbjct: 453 MRSLANICNTGIGKEKM 469
>VPE_VICSA (P49044) Vacuolar processing enzyme precursor (EC 3.4.22.-) (VPE)| (Proteinase B) Length = 493 Score = 169 bits (429), Expect = 5e-42 Identities = 81/149 (54%), Positives = 111/149 (74%), Gaps = 6/149 (4%) Frame = -3 Query: 657 HQFLPTT----NATFVQSSSSSRQL--PGARVNQRDADLVHFWHKYRRSAEGSAEKVEAR 496 +Q+L T N +FV + +S +L P A VNQRDADL+HFW K+R++ EGS++K EA Sbjct: 320 YQYLGTNPANDNNSFVDETENSLKLRTPSAAVNQRDADLIHFWEKFRKAPEGSSQKNEAE 379 Query: 495 RRLVETMARRSRVDSSVELIGGLLFGSEEGAKVLGTVRPAGQPVVDDWGCLKSVVRRFEE 316 ++++E M+ R +D+SV+LIG LLFG E+G ++L VRPAG P+VD+W CLK++V+ FE Sbjct: 380 KQVLEAMSHRKHIDNSVKLIGQLLFGIEKGTELLDVVRPAGSPLVDNWDCLKTMVKTFET 439 Query: 315 RCGPLTQYGMKHMRSLANICNAGVREEVM 229 CG L+QYGMKHMRS ANICNAG+ E M Sbjct: 440 HCGSLSQYGMKHMRSFANICNAGIPNEPM 468
>VPEG_ARATH (Q39119) Vacuolar processing enzyme, gamma-isozyme precursor (EC| 3.4.22.-) (Gamma-VPE) Length = 490 Score = 164 bits (416), Expect = 2e-40 Identities = 76/138 (55%), Positives = 101/138 (73%) Frame = -3 Query: 636 NATFVQSSSSSRQLPGARVNQRDADLVHFWHKYRRSAEGSAEKVEARRRLVETMARRSRV 457 N F + ++S + P NQRDADLVHFW KYR++ EGSA K EA+++++E M+ R + Sbjct: 330 NDNFTFADANSLKPPSRVTNQRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHI 389 Query: 456 DSSVELIGGLLFGSEEGAKVLGTVRPAGQPVVDDWGCLKSVVRRFEERCGPLTQYGMKHM 277 D+SV L+G +LFG G +VL VR AGQP+VDDW CLK+ VR FE CG L+QYG+KHM Sbjct: 390 DNSVILVGKILFGISRGPEVLNKVRSAGQPLVDDWNCLKNQVRAFERHCGSLSQYGIKHM 449 Query: 276 RSLANICNAGVREEVMDK 223 RS ANICNAG++ E M++ Sbjct: 450 RSFANICNAGIQMEQMEE 467
>VPEA_ARATH (P49047) Vacuolar processing enzyme, alpha-isozyme precursor (EC| 3.4.22.-) (Alpha-VPE) Length = 478 Score = 163 bits (413), Expect = 4e-40 Identities = 82/139 (58%), Positives = 107/139 (76%), Gaps = 1/139 (0%) Frame = -3 Query: 636 NATFVQSSSSSRQLPGARV-NQRDADLVHFWHKYRRSAEGSAEKVEARRRLVETMARRSR 460 N TFV +S P +RV NQRDADLVHFWHKY+++ EGSA KVEA+++++E M+ R Sbjct: 321 NFTFVNENSIR---PPSRVTNQRDADLVHFWHKYQKAPEGSARKVEAQKQVLEAMSHRLH 377 Query: 459 VDSSVELIGGLLFGSEEGAKVLGTVRPAGQPVVDDWGCLKSVVRRFEERCGPLTQYGMKH 280 VD+S+ LIG LLFG E G VL VRP+G+P+VDDW CLKS+VR FE CG L+QYG+KH Sbjct: 378 VDNSILLIGILLFGLE-GHAVLNKVRPSGEPLVDDWDCLKSLVRAFERHCGSLSQYGIKH 436 Query: 279 MRSLANICNAGVREEVMDK 223 MRS+AN+CNAG++ M++ Sbjct: 437 MRSIANMCNAGIQMRQMEE 455
>VPE1_PHAVU (O24325) Vacuolar processing enzyme precursor (EC 3.4.22.-) (VPE)| (Legumain-like proteinase) (LLP) Length = 484 Score = 160 bits (405), Expect = 3e-39 Identities = 76/136 (55%), Positives = 99/136 (72%) Frame = -3 Query: 636 NATFVQSSSSSRQLPGARVNQRDADLVHFWHKYRRSAEGSAEKVEARRRLVETMARRSRV 457 N TFV +S VNQRDADLVHFW K+R++ EGS +K EAR++++E M+ R + Sbjct: 326 NLTFVDENSLWSS--SKAVNQRDADLVHFWDKFRKAPEGSPKKNEARKQVLEVMSHRMHI 383 Query: 456 DSSVELIGGLLFGSEEGAKVLGTVRPAGQPVVDDWGCLKSVVRRFEERCGPLTQYGMKHM 277 D SVEL+G LLFG E+ ++L VRPAG +VDDW CLK++VR FE CG L+QYGMKHM Sbjct: 384 DDSVELVGKLLFGIEKAPELLNAVRPAGSALVDDWDCLKTMVRTFETHCGSLSQYGMKHM 443 Query: 276 RSLANICNAGVREEVM 229 RS AN+CN G+++E M Sbjct: 444 RSFANMCNVGIKKEQM 459
>VPE_RICCO (P49042) Vacuolar processing enzyme precursor (EC 3.4.22.-) (VPE)| Length = 497 Score = 139 bits (351), Expect = 6e-33 Identities = 66/120 (55%), Positives = 87/120 (72%) Frame = -3 Query: 582 VNQRDADLVHFWHKYRRSAEGSAEKVEARRRLVETMARRSRVDSSVELIGGLLFGSEEGA 403 VNQRDA+L W Y+RS GS +K E +++ + + RS +DSS++LIG LLFG ++ + Sbjct: 355 VNQRDAELHFMWQLYKRSENGSEKKKEILQQIKDAIKHRSHLDSSMQLIGDLLFGPKKAS 414 Query: 402 KVLGTVRPAGQPVVDDWGCLKSVVRRFEERCGPLTQYGMKHMRSLANICNAGVREEVMDK 223 +L +VR G P+VDDWGCLKS+VR FE CG LTQYGMKHMR+ ANICNAGV M++ Sbjct: 415 AILKSVREPGSPLVDDWGCLKSMVRVFETCCGSLTQYGMKHMRTFANICNAGVSHTSMEE 474
>VPEB_ARATH (Q39044) Vacuolar processing enzyme, beta-isozyme precursor (EC| 3.4.22.-) (Beta-VPE) Length = 486 Score = 139 bits (350), Expect = 8e-33 Identities = 68/120 (56%), Positives = 84/120 (70%) Frame = -3 Query: 582 VNQRDADLVHFWHKYRRSAEGSAEKVEARRRLVETMARRSRVDSSVELIGGLLFGSEEGA 403 VNQRDADL+ WH YR S +GS +K + + L ET R +D+SVELI +LFG Sbjct: 344 VNQRDADLLFLWHMYRTSEDGSRKKDDTLKELTETTRHRKHLDASVELIATILFGPT--M 401 Query: 402 KVLGTVRPAGQPVVDDWGCLKSVVRRFEERCGPLTQYGMKHMRSLANICNAGVREEVMDK 223 VL VR G P+VDDW CLKS+VR FEE CG LTQYGMKHMR+ AN+CN GV +E+M++ Sbjct: 402 NVLNLVREPGLPLVDDWECLKSMVRVFEEHCGSLTQYGMKHMRAFANVCNNGVSKELMEE 461
>VPE_SOYBN (P49045) Vacuolar processing enzyme precursor (EC 3.4.22.-) (VPE)| Length = 495 Score = 135 bits (340), Expect = 1e-31 Identities = 65/120 (54%), Positives = 83/120 (69%) Frame = -3 Query: 582 VNQRDADLVHFWHKYRRSAEGSAEKVEARRRLVETMARRSRVDSSVELIGGLLFGSEEGA 403 VNQRDA+L W Y+RS S K + +++ ET+ R +D SVELIG LL+G +G+ Sbjct: 353 VNQRDAELFLLWQMYQRSNHQSENKTDILKQIAETVKHRKHIDGSVELIGVLLYGPGKGS 412 Query: 402 KVLGTVRPAGQPVVDDWGCLKSVVRRFEERCGPLTQYGMKHMRSLANICNAGVREEVMDK 223 VL +VR G +VDDW CLKS+VR FE CG LTQYGMKHMR+ ANICN+GV E M++ Sbjct: 413 SVLQSVRAPGSSLVDDWTCLKSMVRVFETHCGTLTQYGMKHMRAFANICNSGVSEASMEE 472
>VPE2_PHAVU (O24326) Vacuolar processing enzyme precursor (EC 3.4.22.-)| (Pv-VPE) Length = 493 Score = 134 bits (336), Expect = 3e-31 Identities = 64/120 (53%), Positives = 84/120 (70%) Frame = -3 Query: 582 VNQRDADLVHFWHKYRRSAEGSAEKVEARRRLVETMARRSRVDSSVELIGGLLFGSEEGA 403 VNQRDA+L+ W Y+RS +K + +++ E + R +D SVELIG LL+G E+ + Sbjct: 351 VNQRDAELLFMWQVYQRSNHLPEKKTDILKQIEEIVKHRKHLDGSVELIGVLLYGPEKAS 410 Query: 402 KVLGTVRPAGQPVVDDWGCLKSVVRRFEERCGPLTQYGMKHMRSLANICNAGVREEVMDK 223 VL +VR G P+VDDW CLKS+VR +E CG LTQYGMKHMR+ ANICN+GV E M+K Sbjct: 411 SVLRSVRTTGLPLVDDWTCLKSMVRVYETHCGSLTQYGMKHMRAFANICNSGVSETSMEK 470
>LEGU_CANEN (P49046) Legumain precursor (EC 3.4.22.34) (Asparaginyl| endopeptidase) Length = 475 Score = 130 bits (326), Expect = 5e-30 Identities = 61/120 (50%), Positives = 82/120 (68%) Frame = -3 Query: 582 VNQRDADLVHFWHKYRRSAEGSAEKVEARRRLVETMARRSRVDSSVELIGGLLFGSEEGA 403 VNQRDA+L+ W Y+RS +K ++ ET+ R+ +D SVELIG LL+G + + Sbjct: 333 VNQRDAELLFMWQMYQRSNHQPEKKTHILEQITETVKHRNHLDGSVELIGVLLYGPGKSS 392 Query: 402 KVLGTVRPAGQPVVDDWGCLKSVVRRFEERCGPLTQYGMKHMRSLANICNAGVREEVMDK 223 VL +VR G P+VDDW CLKS+VR FE CG LTQYGMKHMR+ N+CN+GV + M++ Sbjct: 393 SVLHSVRAPGLPLVDDWTCLKSMVRVFETHCGSLTQYGMKHMRAFGNVCNSGVSKASMEE 452
>HM18_CAEEL (P41934) Homeobox protein ceh-18| Length = 542 Score = 33.5 bits (75), Expect = 0.58 Identities = 18/49 (36%), Positives = 27/49 (55%) Frame = +2 Query: 398 TLAPSSEPNRRPPISSTLLSTRDRRAIVSTSRLRASTFSADPSADLRYL 544 +L P NR P +S TL +++ RR TSR+ A++ S D D+ L Sbjct: 251 SLTPKKAENRPPVVSQTLKASK-RRLFDDTSRIEAASMSGDDRIDMNEL 298
>AGRN_HUMAN (O00468) Agrin precursor| Length = 2045 Score = 31.6 bits (70), Expect = 2.2 Identities = 16/30 (53%), Positives = 17/30 (56%) Frame = +2 Query: 347 QPQSSTTGCPAGRTVPRTLAPSSEPNRRPP 436 QP + TT P R P T APS P RRPP Sbjct: 1296 QPVAKTTAAPTTRRPPTT-APSRVPGRRPP 1324
>MUC5B_HUMAN (Q9HC84) Mucin-5B precursor (Mucin 5 subtype B, tracheobronchial)| (High molecular weight salivary mucin MG1) (Sublingual gland mucin) Length = 5703 Score = 31.6 bits (70), Expect = 2.2 Identities = 33/124 (26%), Positives = 44/124 (35%), Gaps = 14/124 (11%) Frame = +2 Query: 275 RMCFI----PYCVSGPHRSSNLRTTDLRQPQSS----------TTGCPAGRTVPRTLAPS 412 +MCF +C + H S T+ P S+ TT T T PS Sbjct: 2926 KMCFNYEIRVFCCNYGHCPSTPATSSTATPSSTPGTTWILTEQTTAATTTATTGSTAIPS 2985 Query: 413 SEPNRRPPISSTLLSTRDRRAIVSTSRLRASTFSADPSADLRYLCQKWTRSASRWFTLAP 592 S P PP + T +TS S+ S + L L T+S + FT P Sbjct: 2986 STPGTAPP---PKVLTSQATTPTATSSKATSSSSPRTATTLPVLTSTATKSTATSFTPIP 3042 Query: 593 GSCL 604 S L Sbjct: 3043 SSTL 3046
>NIRA_EMENI (P28348) Nitrogen assimilation transcription factor nirA| Length = 892 Score = 30.8 bits (68), Expect = 3.8 Identities = 22/66 (33%), Positives = 29/66 (43%) Frame = +2 Query: 320 SNLRTTDLRQPQSSTTGCPAGRTVPRTLAPSSEPNRRPPISSTLLSTRDRRAIVSTSRLR 499 S + TTDL QP S + G P + + PN P++S ST VS R Sbjct: 668 SVVATTDLSQPVSQSAG-----NQPANPSMGTSPNLTQPVASQYSSTPSGPVSVSAMRAV 722 Query: 500 ASTFSA 517 +FSA Sbjct: 723 QRSFSA 728
>DAN4_YEAST (P47179) Cell wall protein DAN4 precursor| Length = 1161 Score = 30.4 bits (67), Expect = 4.9 Identities = 25/99 (25%), Positives = 36/99 (36%) Frame = +2 Query: 233 TSSRTPALQMLASDRMCFIPYCVSGPHRSSNLRTTDLRQPQSSTTGCPAGRTVPRTLAPS 412 T+S TP ++ S +S T SST+ P T P T S Sbjct: 211 TTSTTPTTSTTSTTPTTSTTPTTSTTSTTSQTSTKSTTPTTSSTSTTPTTSTTPTTSTTS 270 Query: 413 SEPNRRPPISSTLLSTRDRRAIVSTSRLRASTFSADPSA 529 + P +++ ST ST+ ST SA S+ Sbjct: 271 TAPTTSTTSTTSTTSTISTAPTTSTTSSTFSTSSASASS 309
>PI5PA_HUMAN (Q15735) Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A (EC| 3.1.3.56) Length = 1006 Score = 30.4 bits (67), Expect = 4.9 Identities = 30/91 (32%), Positives = 39/91 (42%), Gaps = 16/91 (17%) Frame = +2 Query: 218 AALSMTSSRTPALQMLASDRMCFIPYCV------SGPHRSSNLRTTDLRQ---------- 349 AA+S SS P L LAS R P C + HRSS+L T + Q Sbjct: 66 AAMS-ASSEGPRLA-LASPRPILAPLCTPEGQKTATAHRSSSLAPTSVGQLVMSASAGPK 123 Query: 350 PQSSTTGCPAGRTVPRTLAPSSEPNRRPPIS 442 P +TTG T + P+S R PP++ Sbjct: 124 PPPATTGSVLAPTSLGLVMPASAGPRSPPVT 154
>SAMD6_MOUSE (Q6GQX6) Sterile alpha motif domain-containing protein 6| Length = 883 Score = 30.4 bits (67), Expect = 4.9 Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 2/34 (5%) Frame = +3 Query: 345 GSPSHPPQAALPAARCREPWHLP--PSRTGGRRS 440 G PSH P LP ++ R+PW +P P GG +S Sbjct: 417 GRPSHRPP--LPHSKARQPWSIPVLPDDKGGLKS 448
>ISPH2_BURMA (Q62HM8) 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 2 (EC| 1.17.1.2) Length = 326 Score = 30.4 bits (67), Expect = 4.9 Identities = 18/73 (24%), Positives = 35/73 (47%), Gaps = 4/73 (5%) Frame = -3 Query: 588 ARVNQRDADLVHFWHKYRRSAEGSAEKVEARRRLVETMARRSRVD----SSVELIGGLLF 421 A++ Q D+V HK EG+ +VE LVE++ R++ V L+ Sbjct: 122 AKMRQEGVDIVMIGHKGHPEVEGTMGQVERGMHLVESVEDVRRLELPDPERVALVTQTTL 181 Query: 420 GSEEGAKVLGTVR 382 ++ A+++G ++ Sbjct: 182 SVDDAAEIIGALK 194
>ISPH1_BURPS (Q63WH0) 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1 (EC| 1.17.1.2) Length = 326 Score = 30.4 bits (67), Expect = 4.9 Identities = 18/73 (24%), Positives = 35/73 (47%), Gaps = 4/73 (5%) Frame = -3 Query: 588 ARVNQRDADLVHFWHKYRRSAEGSAEKVEARRRLVETMARRSRVD----SSVELIGGLLF 421 A++ Q D+V HK EG+ +VE LVE++ R++ V L+ Sbjct: 122 AKMRQEGVDIVMIGHKGHPEVEGTMGQVERGMHLVESVEDVRRLELPDPERVALVTQTTL 181 Query: 420 GSEEGAKVLGTVR 382 ++ A+++G ++ Sbjct: 182 SVDDAAEIIGALK 194
>SAMD6_RAT (P0C0T2) Sterile alpha motif domain-containing protein 6| (Polycystic kidney disease protein 1) (SamCystin) Length = 871 Score = 30.4 bits (67), Expect = 4.9 Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 2/34 (5%) Frame = +3 Query: 345 GSPSHPPQAALPAARCREPWHLP--PSRTGGRRS 440 G PSH P LP ++ R+PW +P P GG +S Sbjct: 418 GRPSHRPP--LPHSKARQPWSIPMLPDDKGGLKS 449
>SAMD6_HUMAN (Q68DC2) Sterile alpha motif domain-containing protein 6 (Ankyrin| repeat domain-containing protein 14) Length = 871 Score = 30.4 bits (67), Expect = 4.9 Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 2/34 (5%) Frame = +3 Query: 345 GSPSHPPQAALPAARCREPWHLP--PSRTGGRRS 440 G PSH P LP ++ R+PW +P P GG +S Sbjct: 426 GRPSHQPP--LPHSKVRQPWSIPVLPDDKGGLKS 457
>LGMN_HUMAN (Q99538) Legumain precursor (EC 3.4.22.34) (Asparaginyl| endopeptidase) (Protease, cysteine 1) Length = 433 Score = 30.0 bits (66), Expect = 6.5 Identities = 24/92 (26%), Positives = 38/92 (41%), Gaps = 8/92 (8%) Frame = -3 Query: 507 VEARRRLVETMAR----RSRVDSSVELIGGLLFGSEEGAKVLGTVRPAGQPVVDDWGCLK 340 +E R+L E + R R ++ SV I LL SE + L + R + C Sbjct: 325 LEESRQLTEEIQRHLDARHLIEKSVRKIVSLLAASEAEVEQLLSERAP----LTGHSCYP 380 Query: 339 SVVRRFEERC----GPLTQYGMKHMRSLANIC 256 + F C P +Y ++H+ L N+C Sbjct: 381 EALLHFRTHCFNWHSPTYEYALRHLYVLVNLC 412 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 83,518,437 Number of Sequences: 219361 Number of extensions: 1594303 Number of successful extensions: 6074 Number of sequences better than 10.0: 22 Number of HSP's better than 10.0 without gapping: 5785 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 6065 length of database: 80,573,946 effective HSP length: 108 effective length of database: 56,882,958 effective search space used: 6370891296 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
