ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasdk05
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1ERF2_CANMA (Q9HGI7) Eukaryotic peptide chain release factor GTP-... 33 0.68
2MYO9B_HUMAN (Q13459) Myosin-9B (Myosin IXb) (Unconventional myos... 32 1.5
3STAU2_MOUSE (Q8CJ67) Double-stranded RNA-binding protein Staufen... 32 2.0
4SAC3_YEAST (P46674) Leucine permease transcriptional regulator 32 2.6
5TAOK2_RAT (Q9JLS3) Serine/threonine-protein kinase TAO2 (EC 2.7.... 30 7.5
6TAOK2_HUMAN (Q9UL54) Serine/threonine-protein kinase TAO2 (EC 2.... 30 7.5
7V70K_EPMV (P20129) 70 kDa protein 30 7.5
8BCL6_MOUSE (P41183) B-cell lymphoma 6 protein homolog 30 9.8
9ARRC_RANCA (P51481) Arrestin-C (Cone arrestin) 30 9.8

>ERF2_CANMA (Q9HGI7) Eukaryotic peptide chain release factor GTP-binding|
           subunit (ERF2) (Translation release factor 3)
           (Polypeptide release factor 3) (ERF3) (ERF-3)
          Length = 712

 Score = 33.5 bits (75), Expect = 0.68
 Identities = 25/88 (28%), Positives = 37/88 (42%), Gaps = 6/88 (6%)
 Frame = -3

Query: 596 DQEMSAEPDAHGAVSPQKPQDAPGVGQNGLSPTSLSSRTY------LKSASISASKCVVV 435
           D+E SAEP+A    +P     +P       +P  +   +        KSA +SAS  VV 
Sbjct: 204 DEEASAEPEAKKESTPVPASSSPAPAAADSTPAPVKKESTPTPSVASKSAPVSASASVVT 263

Query: 434 NPNKQPEYDDAISEETEGAVDGLSEKVS 351
                 E +D + EE    + G  + VS
Sbjct: 264 ADALAKEQEDEVDEEVVKDMFGGKDHVS 291



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>MYO9B_HUMAN (Q13459) Myosin-9B (Myosin IXb) (Unconventional myosin-9b)|
          Length = 2158

 Score = 32.3 bits (72), Expect = 1.5
 Identities = 31/99 (31%), Positives = 45/99 (45%), Gaps = 1/99 (1%)
 Frame = -3

Query: 599  SDQEMSAEPDAHGAVSPQKPQDAPGVGQNGLSPTSLSSRTYLKSASISASKCVVVNPNKQ 420
            +D E SA+  A   V  +KP DA  +   GLSP S       +  S S  K + ++  K 
Sbjct: 1377 TDGERSAKKPA---VQKKKPGDASSLPDAGLSPGS-------QVDSKSTFKRLFLHKTKD 1426

Query: 419  PEYDDAISEETEGAVDG-LSEKVSKMHA*CSADDGSRHR 306
             +Y    +EE E AV G +  + + M     A  G +HR
Sbjct: 1427 KKYSLEGAEELENAVSGHVVLEATTMKKGLEAPSGQQHR 1465



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>STAU2_MOUSE (Q8CJ67) Double-stranded RNA-binding protein Staufen homolog 2|
          Length = 570

 Score = 32.0 bits (71), Expect = 2.0
 Identities = 26/78 (33%), Positives = 38/78 (48%), Gaps = 4/78 (5%)
 Frame = -3

Query: 632 HR-TTGNNEQHISDQEMSAEPDAHGAVSPQKPQDAPGVGQ---NGLSPTSLSSRTYLKSA 465
           HR T+G    ++S ++M+    +  +VSP     A    +   NG SPT+      LK +
Sbjct: 428 HRVTSGTTLSYLSPKDMNQPSSSFFSVSPSSTSSATVARELLMNGTSPTA--EAIGLKGS 485

Query: 464 SISASKCVVVNPNKQPEY 411
           S   S C  V P+KQ EY
Sbjct: 486 S-PTSPCSSVQPSKQLEY 502



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>SAC3_YEAST (P46674) Leucine permease transcriptional regulator|
          Length = 1301

 Score = 31.6 bits (70), Expect = 2.6
 Identities = 20/77 (25%), Positives = 33/77 (42%)
 Frame = -3

Query: 614 NEQHISDQEMSAEPDAHGAVSPQKPQDAPGVGQNGLSPTSLSSRTYLKSASISASKCVVV 435
           ++ H         P  HG +S QK Q    V   G SP         +++++ ASK  ++
Sbjct: 624 SQSHTLSTNPLLTPQVHGDLSEQKQQQIKTVTDGG-SPFVFDQSA--QNSTVEASKAHMI 680

Query: 434 NPNKQPEYDDAISEETE 384
           +      YD+ +S E E
Sbjct: 681 STTSNGAYDEKLSSEQE 697



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>TAOK2_RAT (Q9JLS3) Serine/threonine-protein kinase TAO2 (EC 2.7.11.1)|
           (Thousand and one amino acid protein 2)
          Length = 1235

 Score = 30.0 bits (66), Expect = 7.5
 Identities = 27/107 (25%), Positives = 46/107 (42%), Gaps = 7/107 (6%)
 Frame = -3

Query: 575 PDAHGAVSPQKPQDAPGVGQNGLSPTSLSSRTYLKSASISAS-------KCVVVNPNKQP 417
           P+  GA +P++ ++A        + TSL S   + S SISAS            + N++ 
Sbjct: 321 PNGPGAEAPEEEEEAEPYMHRAGTLTSLESSHSVPSMSISASSQSSSVNSLADASDNEEE 380

Query: 416 EYDDAISEETEGAVDGLSEKVSKMHA*CSADDGSRHRISHFRQVHRM 276
           E ++   EE E      S +++ M       +G     SH   +HR+
Sbjct: 381 EEEEEEEEEEEEEEGPESREMAMM------QEGEHTVTSHSSIIHRL 421



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>TAOK2_HUMAN (Q9UL54) Serine/threonine-protein kinase TAO2 (EC 2.7.11.1)|
           (Thousand and one amino acid protein 2)
           (Prostate-derived STE20-like kinase 1) (PSK-1) (Kinase
           from chicken homolog C) (hKFC-C)
          Length = 1235

 Score = 30.0 bits (66), Expect = 7.5
 Identities = 26/100 (26%), Positives = 42/100 (42%)
 Frame = -3

Query: 575 PDAHGAVSPQKPQDAPGVGQNGLSPTSLSSRTYLKSASISASKCVVVNPNKQPEYDDAIS 396
           P+  GA +P++ ++A        + TSL S   + S SISAS     + N   +  D   
Sbjct: 321 PNGPGAEAPEEEEEAEPYMHRAGTLTSLESSHSVPSMSISASS-QSSSVNSLADASDNEE 379

Query: 395 EETEGAVDGLSEKVSKMHA*CSADDGSRHRISHFRQVHRM 276
           EE E   +   E+  +        +G     SH   +HR+
Sbjct: 380 EEEEEEEEEEEEEGPEAREMAMMQEGEHTVTSHSSIIHRL 419



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>V70K_EPMV (P20129) 70 kDa protein|
          Length = 649

 Score = 30.0 bits (66), Expect = 7.5
 Identities = 15/30 (50%), Positives = 19/30 (63%)
 Frame = -3

Query: 506 SPTSLSSRTYLKSASISASKCVVVNPNKQP 417
           SP+SLS RT+L SA IS     V+ P+  P
Sbjct: 546 SPSSLSLRTFLDSAVISCDSSPVLPPSPSP 575



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>BCL6_MOUSE (P41183) B-cell lymphoma 6 protein homolog|
          Length = 707

 Score = 29.6 bits (65), Expect = 9.8
 Identities = 34/129 (26%), Positives = 54/129 (41%), Gaps = 16/129 (12%)
 Frame = -1

Query: 460 LVLPSVSLSTRINSQSMTMRSLRKRKGPS--TDYPRRSPRCMPSAAP-----MTEVGTAF 302
           LV  S++ S R +S+S +   +   K  S  +  P+ +  C+ +A P     M E  + +
Sbjct: 450 LVNRSLAGSPRSSSESHSPLYMHPPKCTSCGSQSPQHTEMCLHTAGPTFPEEMGETQSEY 509

Query: 301 LISVRYTG--------C**QSSAVLGKHCIQV-GDCLASV*SCQASLLHAGTVALHGLMY 149
             S    G        C     A L +H +Q   D       CQAS  + G +A H  ++
Sbjct: 510 SDSSCENGTFFCNECDCRFSEEASLKRHTLQTHSDKPYKCDRCQASFRYKGNLASHKTVH 569

Query: 148 FYTQDYRCN 122
              + YRCN
Sbjct: 570 TGEKPYRCN 578



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>ARRC_RANCA (P51481) Arrestin-C (Cone arrestin)|
          Length = 389

 Score = 29.6 bits (65), Expect = 9.8
 Identities = 25/102 (24%), Positives = 43/102 (42%), Gaps = 5/102 (4%)
 Frame = +2

Query: 29  ECSTAKQDSVQMSCSVRYNHA*MICVGTNPGVTSVILSVKIHQPMQGNSSC---VQQRCL 199
           E    K+  V M+C+ RY    M  +G +      + S ++H P+ G       +Q++  
Sbjct: 45  EYQKEKKVFVMMTCAFRYGRDDMELIGLSFRKDIYVQSCQVHPPLPGEKKALTPLQEKLK 104

Query: 200 A--GSHTR*TISNLYAVLP*DRTALLSASGVPDGNEKCGADF 319
           A  G ++     N+   LP    ++    G  D  + CG DF
Sbjct: 105 AKLGGNSFPFSFNMATNLP---CSVTLQPGPEDSGKACGVDF 143


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 106,736,500
Number of Sequences: 219361
Number of extensions: 2284214
Number of successful extensions: 6502
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 6100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6498
length of database: 80,573,946
effective HSP length: 109
effective length of database: 56,663,597
effective search space used: 7422931207
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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