ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasdb10
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1IM30_ORYSA (Q8S0J7) Probable membrane-associated 30 kDa protein,... 107 9e-38
2IM30_PEA (Q03943) Membrane-associated 30 kDa protein, chloroplas... 112 8e-35
3IM30_ARATH (O80796) Probable membrane-associated 30 kDa protein,... 95 2e-32
4Y617_SYNY3 (Q55707) Hypothetical protein sll0617 61 3e-09
5PSPA_DEIRA (Q9RUB7) Phage shock protein A homolog 34 0.45
6PSPA_BACSU (P54617) Phage shock protein A homolog 33 0.76
7STCK_EMENI (Q00706) Putative sterigmatocystin biosynthesis fatty... 31 3.8
8EKC1_SCHPO (O74511) Extragenic suppressor of kinetochore protein 1 30 4.9
9SMG5_HUMAN (Q9UPR3) Protein SMG5 (SMG-5 homolog) (EST1-like prot... 30 4.9
10TRUB_PSEF5 (Q4KIF4) tRNA pseudouridine synthase B (EC 5.4.99.-) ... 30 8.4
11POL_MLVAV (P03356) Pol polyprotein [Contains: Protease (EC 3.4.2... 30 8.4

>IM30_ORYSA (Q8S0J7) Probable membrane-associated 30 kDa protein, chloroplast|
           precursor
          Length = 317

 Score =  107 bits (266), Expect(2) = 9e-38
 Identities = 55/62 (88%), Positives = 58/62 (93%)
 Frame = -3

Query: 661 AQSAKTATKVSEMLGNVNTSSALSAFEKMEEKVMTMESQAEALGQLGADDLEGKFAMLET 482
           AQSAKT+TKVSEMLGNVNTS ALSAFEKMEEKVM MESQAEALGQL  DDLEGKFA+LET
Sbjct: 200 AQSAKTSTKVSEMLGNVNTSGALSAFEKMEEKVMAMESQAEALGQLATDDLEGKFALLET 259

Query: 481 TS 476
           +S
Sbjct: 260 SS 261



 Score = 70.1 bits (170), Expect(2) = 9e-38
 Identities = 38/61 (62%), Positives = 43/61 (70%), Gaps = 8/61 (13%)
 Frame = -1

Query: 489 LRLHRVDDDLAQMRKELSGSSLKGELPQGRTATP--------RDRDIERELNELREKAND 334
           L    VDDDLAQM+KE+SGSS KGELP GRTA          RD +IE ELNELR+KAN+
Sbjct: 257 LETSSVDDDLAQMKKEISGSSSKGELPPGRTAVSNSGAARPFRDIEIENELNELRKKANE 316

Query: 333 Y 331
           Y
Sbjct: 317 Y 317



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>IM30_PEA (Q03943) Membrane-associated 30 kDa protein, chloroplast precursor|
           (M30)
          Length = 323

 Score =  112 bits (279), Expect(2) = 8e-35
 Identities = 59/62 (95%), Positives = 60/62 (96%)
 Frame = -3

Query: 661 AQSAKTATKVSEMLGNVNTSSALSAFEKMEEKVMTMESQAEALGQLGADDLEGKFAMLET 482
           AQSAKTATKVSEMLGNVNTSSALSAFEKMEEKVMTMESQAEALGQL  DDLEGKFAMLET
Sbjct: 203 AQSAKTATKVSEMLGNVNTSSALSAFEKMEEKVMTMESQAEALGQLTTDDLEGKFAMLET 262

Query: 481 TS 476
           +S
Sbjct: 263 SS 264



 Score = 55.1 bits (131), Expect(2) = 8e-35
 Identities = 30/63 (47%), Positives = 40/63 (63%), Gaps = 11/63 (17%)
 Frame = -1

Query: 489 LRLHRVDDDLAQMRKELSGSSLKGELPQGRTATP-----------RDRDIERELNELREK 343
           L    VDDDLA ++KEL+GSS KGELP GR++T            RD DIE EL +LR++
Sbjct: 260 LETSSVDDDLANLKKELAGSSKKGELPPGRSSTTSTTSTKTGNPFRDADIEIELEQLRKR 319

Query: 342 AND 334
           + +
Sbjct: 320 SKE 322



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>IM30_ARATH (O80796) Probable membrane-associated 30 kDa protein, chloroplast|
           precursor
          Length = 330

 Score = 94.7 bits (234), Expect(2) = 2e-32
 Identities = 47/62 (75%), Positives = 55/62 (88%)
 Frame = -3

Query: 661 AQSAKTATKVSEMLGNVNTSSALSAFEKMEEKVMTMESQAEALGQLGADDLEGKFAMLET 482
           A++AKTATKV EM+G VNTS ALSAFEKMEEKVM MES+A+AL Q+G D+LEGKF MLET
Sbjct: 214 ARTAKTATKVQEMIGTVNTSGALSAFEKMEEKVMAMESEADALTQIGTDELEGKFQMLET 273

Query: 481 TS 476
           +S
Sbjct: 274 SS 275



 Score = 64.3 bits (155), Expect(2) = 2e-32
 Identities = 34/60 (56%), Positives = 40/60 (66%), Gaps = 7/60 (11%)
 Frame = -1

Query: 489 LRLHRVDDDLAQMRKELSGSSLKGELPQGRTATP-------RDRDIERELNELREKANDY 331
           L    VDDDLA ++KELSGSS KGELP GR+          +D +IE ELNELR KAND+
Sbjct: 271 LETSSVDDDLADLKKELSGSSKKGELPPGRSTVAASTRYPFKDSEIENELNELRRKANDF 330



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>Y617_SYNY3 (Q55707) Hypothetical protein sll0617|
          Length = 267

 Score = 60.8 bits (146), Expect = 3e-09
 Identities = 29/63 (46%), Positives = 44/63 (69%)
 Frame = -3

Query: 661 AQSAKTATKVSEMLGNVNTSSALSAFEKMEEKVMTMESQAEALGQLGADDLEGKFAMLET 482
           A++AK   ++ + LG + TSSA SAFE+ME KV+ ME+ ++A G+L    +E +FA LE 
Sbjct: 143 AKAAKANAELQQTLGGLGTSSATSAFERMENKVLDMEATSQAAGELAGFGIENQFAQLEA 202

Query: 481 TSG 473
           +SG
Sbjct: 203 SSG 205



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>PSPA_DEIRA (Q9RUB7) Phage shock protein A homolog|
          Length = 223

 Score = 33.9 bits (76), Expect = 0.45
 Identities = 19/49 (38%), Positives = 29/49 (59%)
 Frame = -3

Query: 661 AQSAKTATKVSEMLGNVNTSSALSAFEKMEEKVMTMESQAEALGQLGAD 515
           AQ+ +T  +VS   G      A+ AF +ME+KV  ME + +A+G+L  D
Sbjct: 146 AQAGETLDRVS---GFSKAGGAMDAFNEMEQKVAGMEDRNKAMGELRND 191



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>PSPA_BACSU (P54617) Phage shock protein A homolog|
          Length = 226

 Score = 33.1 bits (74), Expect = 0.76
 Identities = 21/61 (34%), Positives = 37/61 (60%), Gaps = 4/61 (6%)
 Frame = -3

Query: 652 AKTATKVSEMLGNVN-TSSALSAFEKMEEKVMTMESQAEALGQLGA---DDLEGKFAMLE 485
           AKT  +++++  +V+ TS ++SAF +ME+KV     QA A+ +L +   DD+    A  +
Sbjct: 145 AKTQERMNKLGASVSSTSQSMSAFGRMEDKVNKALDQANAMAELNSAPQDDMADLSAKYD 204

Query: 484 T 482
           T
Sbjct: 205 T 205



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>STCK_EMENI (Q00706) Putative sterigmatocystin biosynthesis fatty acid synthase|
            beta subunit
          Length = 1914

 Score = 30.8 bits (68), Expect = 3.8
 Identities = 19/65 (29%), Positives = 31/65 (47%), Gaps = 2/65 (3%)
 Frame = -1

Query: 405  GRTATPRDRDIERELNEL--REKANDY*ILQGMPVYRAGRDLLSQRTPEKRIACRFVQCA 232
            G T T   R  ER +  +  R + +   + +G PV R     + QR PE+ ++ +  +C 
Sbjct: 1239 GDTVTTSSRITERTITTIGQRVEISAELLREGKPVVRLQTTFIIQRRPEESVSQQQFRCV 1298

Query: 231  FEPSM 217
             EP M
Sbjct: 1299 EEPDM 1303



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>EKC1_SCHPO (O74511) Extragenic suppressor of kinetochore protein 1|
          Length = 838

 Score = 30.4 bits (67), Expect = 4.9
 Identities = 16/46 (34%), Positives = 25/46 (54%)
 Frame = -1

Query: 471 DDDLAQMRKELSGSSLKGELPQGRTATPRDRDIERELNELREKAND 334
           DDD+ ++ +ELS +S   +   G    P  R++ R L+   E AND
Sbjct: 697 DDDVVEVHRELSHNSSSNDEDDGNDEDPLSREMSRRLS--FESAND 740



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>SMG5_HUMAN (Q9UPR3) Protein SMG5 (SMG-5 homolog) (EST1-like protein B)|
            (LPTS-interacting protein) (LPTS-RP1)
          Length = 1016

 Score = 30.4 bits (67), Expect = 4.9
 Identities = 26/103 (25%), Positives = 45/103 (43%), Gaps = 8/103 (7%)
 Frame = -1

Query: 486  RLHRVDDDLAQMRKELSGSSLKGELPQGRTAT-------PRDRDIERELNELREKANDY* 328
            R +R+  D+AQ+R +L  S L+G L Q +  +       P  + +   L  +R+ A    
Sbjct: 820  RRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGR 879

Query: 327  ILQGMP-VYRAGRDLLSQRTPEKRIACRFVQCAFEPSMHVFFC 202
             +  +P     G DLL +  P  R   R+++  F+       C
Sbjct: 880  FIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRC 922



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>TRUB_PSEF5 (Q4KIF4) tRNA pseudouridine synthase B (EC 5.4.99.-) (tRNA|
           pseudouridine 55 synthase) (Psi55 synthase)
           (tRNA-uridine isomerase) (tRNA pseudouridylate synthase)
          Length = 305

 Score = 29.6 bits (65), Expect = 8.4
 Identities = 27/88 (30%), Positives = 39/88 (44%), Gaps = 7/88 (7%)
 Frame = -1

Query: 468 DDLAQMRKELSGSSLKGELPQGRTATPRDRDIERELNELREKAND----Y*ILQ--GMPV 307
           + LAQ+ K  + +  +GE+ Q R  T    DIE  L E R +       Y  L+  G P+
Sbjct: 77  ETLAQLGKTTTTADAEGEVLQTREVTVGRADIEAVLPEFRGEIKQIPPMYSALKRDGQPL 136

Query: 306 YRAGR-DLLSQRTPEKRIACRFVQCAFE 226
           Y+  R   + +R P      R    AFE
Sbjct: 137 YKLARAGEVVEREPRSVTIARLELLAFE 164



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>POL_MLVAV (P03356) Pol polyprotein [Contains: Protease (EC 3.4.23.-); Reverse|
           transcriptase/ribonuclease H (EC 2.7.7.49) (EC 3.1.26.4)
           (RT); Integrase (IN)]
          Length = 1196

 Score = 29.6 bits (65), Expect = 8.4
 Identities = 21/70 (30%), Positives = 31/70 (44%), Gaps = 7/70 (10%)
 Frame = -1

Query: 507 KESLLCLRLHRVDDDLAQMRKELSGSSLKGE-----LPQGRTATPR--DRDIERELNELR 349
           K++  CLRLH     L        G  + G+     LPQG   +P   D  + R+L + R
Sbjct: 272 KDAFFCLRLHPTSQPLFAFEWRDPGMGISGQLTWTRLPQGFKNSPTLFDEALHRDLADFR 331

Query: 348 EKANDY*ILQ 319
            +  D  +LQ
Sbjct: 332 IQHPDLILLQ 341


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 93,929,820
Number of Sequences: 219361
Number of extensions: 1919338
Number of successful extensions: 4636
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 4490
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4634
length of database: 80,573,946
effective HSP length: 108
effective length of database: 56,882,958
effective search space used: 6370891296
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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