ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd3n09
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1SAP_CHICK (O13035) Proactivator polypeptide precursor [Contains:... 45 1e-04
2SAP_RAT (P10960) Sulfated glycoprotein 1 precursor (SGP-1) (Pros... 38 0.021
3SAP_HUMAN (P07602) Proactivator polypeptide precursor [Contains:... 38 0.021
4SAP_BOVIN (P26779) Proactivator polypeptide precursor [Contains:... 37 0.036
5SAP_MOUSE (Q61207) Sulfated glycoprotein 1 precursor (SGP-1) (Pr... 37 0.061
6OXAA_DEIRA (Q9RSH5) Membrane protein oxaA 34 0.40
7NF1_RAT (P97526) Neurofibromin (Neurofibromatosis-related protei... 32 1.2
8NF1_HUMAN (P21359) Neurofibromin (Neurofibromatosis-related prot... 32 1.2
9NF1_MOUSE (Q04690) Neurofibromin (Neurofibromatosis-related prot... 32 1.2
10SYP32_ARATH (Q9LK09) Syntaxin-32 (AtSYP32) 30 7.5
11SEN54_SCHPO (O74908) Probable tRNA-splicing endonuclease subunit... 30 7.5
12GLRX1_YEAST (P25373) Glutaredoxin-1 (Glutathione-dependent oxido... 30 7.5
13YGEZ_ECOLI (Q46806) Hypothetical hydrolase ygeZ (EC 3.-.-.-) 29 9.8
14FDHD_BACLD (Q65DY4) Protein fdhD homolog 29 9.8
15DSG3_MOUSE (O35902) Desmoglein-3 precursor (130 kDa pemphigus vu... 29 9.8

>SAP_CHICK (O13035) Proactivator polypeptide precursor [Contains: Saposin A;|
           Saposin B; Saposin C; Saposin D]
          Length = 518

 Score = 45.4 bits (106), Expect = 1e-04
 Identities = 31/123 (25%), Positives = 58/123 (47%), Gaps = 3/123 (2%)
 Frame = -3

Query: 603 CRDNALLSGVRSESTCVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKI--EGHEQQCK 430
           C+ N       +   C  C  L+  V   LKD   E EI   L K C+ +  +G   +CK
Sbjct: 49  CQQNVWSKPAVNSIPCDLCKELVTVVGKVLKDNGTEDEIRSYLEKRCEFLPDQGLASECK 108

Query: 429 RLVLQYIPLIL-VNGEKFLEKNDICTIVQACDAGKKTVLGSFSEEGLLRDE*RQKDYTEP 253
            +V  Y+P+I+ +  E+F +   +C+ +  C + +K +     ++ L  ++  + D++E 
Sbjct: 109 EIVDSYLPVIMDMIKEEFDKPEVVCSALSLCQSLQKHLAAMKLQKQLQSNKIPELDFSEL 168

Query: 252 RKP 244
             P
Sbjct: 169 TSP 171



 Score = 37.0 bits (84), Expect = 0.047
 Identities = 23/83 (27%), Positives = 39/83 (46%), Gaps = 1/83 (1%)
 Frame = -3

Query: 558 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKI-EGHEQQCKRLVLQYIPLILVNGEK 382
           C  C  ++     +L+      EI  +L K C  + E    QC + V QY P+++    +
Sbjct: 403 CDICKMIVAYADKELEKNATTTEIEALLEKVCHFLPESVSDQCVQFVEQYEPVVVQLLAE 462

Query: 381 FLEKNDICTIVQACDAGKKTVLG 313
            ++   +CT +  C A KK +LG
Sbjct: 463 MMDPTFVCTKLGVCGAAKKPLLG 485



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>SAP_RAT (P10960) Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)|
          Length = 554

 Score = 38.1 bits (87), Expect = 0.021
 Identities = 21/84 (25%), Positives = 43/84 (51%), Gaps = 1/84 (1%)
 Frame = -3

Query: 558 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKI-EGHEQQCKRLVLQYIPLILVNGEK 382
           C  C  L+  +   L+    + EI+  L K C  + + +++QC   V +Y PL+L    +
Sbjct: 439 CEVCKKLVIYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEPLLLEILVE 498

Query: 381 FLEKNDICTIVQACDAGKKTVLGS 310
            ++ + +C+ +  C +  K +LG+
Sbjct: 499 VMDPSFVCSKIGVCPSAYKLLLGT 522



 Score = 37.0 bits (84), Expect = 0.047
 Identities = 24/79 (30%), Positives = 37/79 (46%), Gaps = 4/79 (5%)
 Frame = -3

Query: 558 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKIEGH--EQQCKRLVLQYIPLIL--VN 391
           C  C  ++ E  + LKD   E EI+  L K C  I        CK +V  Y+P+IL  + 
Sbjct: 63  CDICKTVVTEAGNLLKDNATEEEILHYLEKTCAWIHDSSLSASCKEVVDSYLPVILDMIK 122

Query: 390 GEKFLEKNDICTIVQACDA 334
           GE      ++C+ +  C +
Sbjct: 123 GE-MSNPGEVCSALNLCQS 140



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>SAP_HUMAN (P07602) Proactivator polypeptide precursor [Contains: Saposin A|
           (Protein A); Saposin B-Val; Saposin B (Sphingolipid
           activator protein 1) (SAP-1) (Cerebroside sulfate
           activator) (CSAct) (Dispersin) (Sulfatide/GM1
           activator); Saposin C (Co-beta-
          Length = 524

 Score = 38.1 bits (87), Expect = 0.021
 Identities = 20/84 (23%), Positives = 44/84 (52%), Gaps = 1/84 (1%)
 Frame = -3

Query: 558 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKI-EGHEQQCKRLVLQYIPLILVNGEK 382
           C  C  L+  +   L+    + EI+  L K C  + + +++QC + V +Y P+++    +
Sbjct: 409 CEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVE 468

Query: 381 FLEKNDICTIVQACDAGKKTVLGS 310
            ++ + +C  + AC +  K +LG+
Sbjct: 469 VMDPSFVCLKIGACPSAHKPLLGT 492



 Score = 36.2 bits (82), Expect = 0.080
 Identities = 28/109 (25%), Positives = 49/109 (44%), Gaps = 4/109 (3%)
 Frame = -3

Query: 558 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKI--EGHEQQCKRLVLQYIPLIL--VN 391
           C  C  ++      LKD   E EI+  L K C  +        CK +V  Y+P+IL  + 
Sbjct: 63  CDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIK 122

Query: 390 GEKFLEKNDICTIVQACDAGKKTVLGSFSEEGLLRDE*RQKDYTEPRKP 244
           GE      ++C+ +  C++ +K +     ++ L  ++  + D TE   P
Sbjct: 123 GE-MSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIPELDMTEVVAP 170



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>SAP_BOVIN (P26779) Proactivator polypeptide precursor [Contains: Saposin A|
           (Protein A); Saposin B (Sphingolipid activator protein
           1) (SAP-1) (Cerebroside sulfate activator) (CSAct)
           (Dispersin) (Sulfatide/GM1 activator); Saposin C
           (Co-beta-glucosidase) (A
          Length = 525

 Score = 37.4 bits (85), Expect = 0.036
 Identities = 20/84 (23%), Positives = 43/84 (51%), Gaps = 1/84 (1%)
 Frame = -3

Query: 558 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKI-EGHEQQCKRLVLQYIPLILVNGEK 382
           C  C  L+  +   L+    + +I+  L K C  + + + +QC + V +Y P+++    +
Sbjct: 410 CEVCKKLVGYLDRNLEKNSTKEQILAALEKGCSFLPDQYRKQCDQFVTEYEPVLIEILVE 469

Query: 381 FLEKNDICTIVQACDAGKKTVLGS 310
            ++ + +C  + AC A  K +LG+
Sbjct: 470 VMDPSFVCLKIGACPAAHKPLLGA 493



 Score = 33.5 bits (75), Expect = 0.52
 Identities = 21/82 (25%), Positives = 39/82 (47%), Gaps = 4/82 (4%)
 Frame = -3

Query: 558 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKIE--GHEQQCKRLVLQYIPLIL--VN 391
           C  C  ++    + LKD   E EI+  L + C  +        CK +V  Y+P+IL  + 
Sbjct: 63  CDICKDVITAAGNLLKDNATEQEILMYLERTCDWLPKPNMSASCKEIVDSYLPVILDMIK 122

Query: 390 GEKFLEKNDICTIVQACDAGKK 325
           G+      ++C+ +  C++ +K
Sbjct: 123 GQ-MSHPGEVCSALNLCESLQK 143



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>SAP_MOUSE (Q61207) Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)|
          Length = 557

 Score = 36.6 bits (83), Expect = 0.061
 Identities = 21/84 (25%), Positives = 42/84 (50%), Gaps = 1/84 (1%)
 Frame = -3

Query: 558 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKI-EGHEQQCKRLVLQYIPLILVNGEK 382
           C  C  L+  +   L+    + EI+  L K C  + + +++QC   V +Y PL+L    +
Sbjct: 442 CEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVE 501

Query: 381 FLEKNDICTIVQACDAGKKTVLGS 310
            ++   +C+ +  C +  K +LG+
Sbjct: 502 VMDPGFVCSKIGVCPSAYKLLLGT 525



 Score = 36.6 bits (83), Expect = 0.061
 Identities = 23/79 (29%), Positives = 38/79 (48%), Gaps = 4/79 (5%)
 Frame = -3

Query: 558 CVFCHHLMDEVLSKLKDPDAEFEIIQILLKECKKIEGH--EQQCKRLVLQYIPLIL--VN 391
           C  C  ++ E  + LKD   + EI+  L K C+ I        CK +V  Y+P+IL  + 
Sbjct: 63  CDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIK 122

Query: 390 GEKFLEKNDICTIVQACDA 334
           GE      ++C+ +  C +
Sbjct: 123 GE-MSNPGEVCSALNLCQS 140



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>OXAA_DEIRA (Q9RSH5) Membrane protein oxaA|
          Length = 428

 Score = 33.9 bits (76), Expect = 0.40
 Identities = 16/40 (40%), Positives = 24/40 (60%)
 Frame = +1

Query: 184 ASRILDTYVQQEELVHYVSSGFTRLGVVFLPSFIAQQTLL 303
           A+  L+ Y  + EL+H   SG+T L  +F P+F  Q +LL
Sbjct: 159 ATSSLEVYGGKNELIHLYQSGYTELPGLFQPNFFGQISLL 198



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>NF1_RAT (P97526) Neurofibromin (Neurofibromatosis-related protein NF-1)|
          Length = 2820

 Score = 32.3 bits (72), Expect = 1.2
 Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 5/64 (7%)
 Frame = -3

Query: 543 HLMDEVLSKLKDPDAEFEII-----QILLKECKKIEGHEQQCKRLVLQYIPLILVNGEKF 379
           H +D +   L +PDA  E       Q+L   CKK+  H+      +L+++  IL+   KF
Sbjct: 553 HQLDSI--DLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKF 610

Query: 378 LEKN 367
           L KN
Sbjct: 611 LLKN 614



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>NF1_HUMAN (P21359) Neurofibromin (Neurofibromatosis-related protein NF-1)|
           [Contains: Neurofibromin truncated]
          Length = 2839

 Score = 32.3 bits (72), Expect = 1.2
 Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 5/64 (7%)
 Frame = -3

Query: 543 HLMDEVLSKLKDPDAEFEII-----QILLKECKKIEGHEQQCKRLVLQYIPLILVNGEKF 379
           H +D +   L +PDA  E       Q+L   CKK+  H+      +L+++  IL+   KF
Sbjct: 553 HQLDSI--DLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKF 610

Query: 378 LEKN 367
           L KN
Sbjct: 611 LLKN 614



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>NF1_MOUSE (Q04690) Neurofibromin (Neurofibromatosis-related protein NF-1)|
          Length = 2841

 Score = 32.3 bits (72), Expect = 1.2
 Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 5/64 (7%)
 Frame = -3

Query: 543 HLMDEVLSKLKDPDAEFEII-----QILLKECKKIEGHEQQCKRLVLQYIPLILVNGEKF 379
           H +D +   L +PDA  E       Q+L   CKK+  H+      +L+++  IL+   KF
Sbjct: 553 HQLDSI--DLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKF 610

Query: 378 LEKN 367
           L KN
Sbjct: 611 LLKN 614



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>SYP32_ARATH (Q9LK09) Syntaxin-32 (AtSYP32)|
          Length = 347

 Score = 29.6 bits (65), Expect = 7.5
 Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
 Frame = +1

Query: 139 IIQSIQRMQQQHAAKASRILDTYVQ-QEELVHYVSSGFTRLGVVF--LPSFIAQQ 294
           ++Q  Q+ QQQ   +   + DTY+Q + E +H V S    L  +F  L + ++QQ
Sbjct: 235 LLQQSQQQQQQQQQQMVPLQDTYMQGRAEALHTVESTIHELSSIFTQLATMVSQQ 289



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>SEN54_SCHPO (O74908) Probable tRNA-splicing endonuclease subunit sen54|
           (tRNA-intron endonuclease sen54)
          Length = 384

 Score = 29.6 bits (65), Expect = 7.5
 Identities = 11/32 (34%), Positives = 19/32 (59%)
 Frame = -2

Query: 382 VPREERYMHHRSSMRCWQKDGARVILRGGFAA 287
           +P E  Y+  R SM CW ++G  + L+  ++A
Sbjct: 120 LPEETLYLVERGSMECWSEEGLPMSLQAVYSA 151



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>GLRX1_YEAST (P25373) Glutaredoxin-1 (Glutathione-dependent oxidoreductase 1)|
          Length = 110

 Score = 29.6 bits (65), Expect = 7.5
 Identities = 21/75 (28%), Positives = 40/75 (53%), Gaps = 3/75 (4%)
 Frame = -3

Query: 576 VRSESTCVFCHHLMDEVLSKLKDPDAEFEIIQIL-LKECKKIEG--HEQQCKRLVLQYIP 406
           V S++ C +CH  ++ +  KLK P ++  ++Q+  +KE   I+   +E   +R V    P
Sbjct: 21  VASKTYCPYCHAALNTLFEKLKVPRSKVLVLQLNDMKEGADIQAALYEINGQRTV----P 76

Query: 405 LILVNGEKFLEKNDI 361
            I +NG+     +D+
Sbjct: 77  NIYINGKHIGGNDDL 91



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>YGEZ_ECOLI (Q46806) Hypothetical hydrolase ygeZ (EC 3.-.-.-)|
          Length = 461

 Score = 29.3 bits (64), Expect = 9.8
 Identities = 23/69 (33%), Positives = 27/69 (39%)
 Frame = +3

Query: 27  FSIYSLFRYHYKLNTDQVISTTRLQVDIRNCSSYRSLYNTKHPENAAAARSKSQSNFGHL 206
           F +Y    Y YKLN D+V+   R   +            T HPEN AA  SK        
Sbjct: 150 FKLY--LTYQYKLNDDEVLQALRRLHE-------SGALTTVHPENDAAIASKRAEFIAAG 200

Query: 207 CTTRRTSAL 233
            T  R  AL
Sbjct: 201 LTAPRYHAL 209



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>FDHD_BACLD (Q65DY4) Protein fdhD homolog|
          Length = 263

 Score = 29.3 bits (64), Expect = 9.8
 Identities = 17/61 (27%), Positives = 36/61 (59%)
 Frame = -3

Query: 441 QQCKRLVLQYIPLILVNGEKFLEKNDICTIVQACDAGKKTVLGSFSEEGLLRDE*RQKDY 262
           QQ  ++  ++   ++VNGE+F+      T+V + D+ ++ V+G  + EG++R +   K +
Sbjct: 21  QQEDQMATEFPLTVMVNGEEFV------TLVCSPDSLEELVIGFLASEGVIRFKNEIKRF 74

Query: 261 T 259
           T
Sbjct: 75  T 75



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>DSG3_MOUSE (O35902) Desmoglein-3 precursor (130 kDa pemphigus vulgaris antigen|
           homolog)
          Length = 993

 Score = 29.3 bits (64), Expect = 9.8
 Identities = 11/34 (32%), Positives = 20/34 (58%)
 Frame = -3

Query: 456 IEGHEQQCKRLVLQYIPLILVNGEKFLEKNDICT 355
           IEG + + K +    +P +  NG  F+E +++CT
Sbjct: 671 IEGAQPEDKEITNICVPPVTTNGADFMESSEVCT 704


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 87,570,431
Number of Sequences: 219361
Number of extensions: 1761568
Number of successful extensions: 4992
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 4831
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4989
length of database: 80,573,946
effective HSP length: 107
effective length of database: 57,102,319
effective search space used: 5653129581
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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