ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd3n06
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1RKIN1_SECCE (Q02723) Carbon catabolite derepressing protein kina... 286 6e-77
2KIN10_ARATH (Q38997) SNF1-related protein kinase catalytic alpha... 211 2e-54
3KIN11_ARATH (P92958) SNF1-related protein kinase catalytic alpha... 187 2e-47
4AAPK2_CAEEL (Q95ZQ4) 5'-AMP-activated protein kinase catalytic s... 45 2e-04
5SNF1_CANGA (Q00372) Carbon catabolite derepressing protein kinas... 45 3e-04
6SNF1_YEAST (P06782) Carbon catabolite derepressing protein kinas... 44 4e-04
7AAPK2_RAT (Q09137) 5'-AMP-activated protein kinase catalytic sub... 42 0.002
8AAPK2_PONPY (Q5RD00) 5'-AMP-activated protein kinase catalytic s... 42 0.002
9AAPK2_PIG (Q28948) 5'-AMP-activated protein kinase catalytic sub... 42 0.002
10AAPK2_HUMAN (P54646) 5'-AMP-activated protein kinase catalytic s... 42 0.002
11SNF1_CANAL (P52497) Carbon catabolite derepressing protein kinas... 42 0.002
12AAPK1_PONPY (Q5RDH5) 5'-AMP-activated protein kinase catalytic s... 42 0.002
13AAPK1_HUMAN (Q13131) 5'-AMP-activated protein kinase catalytic s... 42 0.002
14AAPK1_RAT (P54645) 5'-AMP-activated protein kinase catalytic sub... 42 0.002
15AAPK1_MOUSE (Q5EG47) 5'-AMP-activated protein kinase catalytic s... 42 0.002
16SNF1_CANTR (O94168) Carbon catabolite derepressing protein kinas... 40 0.007
17AHPF_PSEPU (P0A156) Alkyl hydroperoxide reductase subunit F (EC ... 31 4.2
18AHPF_PSEPK (P0A155) Alkyl hydroperoxide reductase subunit F (EC ... 31 4.2
19CDC23_YEAST (P16522) Anaphase-promoting complex subunit CDC23 (C... 31 4.2
20SYS_FUSNN (Q8RH11) Seryl-tRNA synthetase (EC 6.1.1.11) (Serine--... 30 5.5
21K0329_HUMAN (O15040) Protein KIAA0329/KIAA0297 30 9.4

>RKIN1_SECCE (Q02723) Carbon catabolite derepressing protein kinase (EC|
           2.7.11.1)
          Length = 502

 Score =  286 bits (731), Expect = 6e-77
 Identities = 149/179 (83%), Positives = 154/179 (86%), Gaps = 3/179 (1%)
 Frame = -3

Query: 702 RATSGYLGADYQQSMGRSLNQFTSSESASPSTRQYLPGTNDSQGSGLRPYYPVERKWALG 523
           RATSGYLGA YQQ M          ESASPSTR YLPG+NDSQGSGLRPYY VERKWALG
Sbjct: 334 RATSGYLGAHYQQPM----------ESASPSTRSYLPGSNDSQGSGLRPYYRVERKWALG 383

Query: 522 LQ-SRAHPREIMIEVLKALQELNVSWKKNGH-YNMKCRWCPGFPQVSDML-DANHSFVDD 352
           LQ SRA PR IMIEVLKAL+ELNV WKKNG  YNMKCRWCPGFP+VSDML DANHSFVDD
Sbjct: 384 LQQSRAPPRAIMIEVLKALKELNVCWKKNGDCYNMKCRWCPGFPRVSDMLLDANHSFVDD 443

Query: 351 STIMDNGDANGRLPAVIKFEIQLYKTKDDKYLLDMQRLTGPQLLFLDFCAAFLTNLRVL 175
             I DNGDAN RLPAVIKFEIQLYKTKDDKYLLDMQR+TGPQLLFL+FCAAFLTNLRVL
Sbjct: 444 CAIKDNGDANSRLPAVIKFEIQLYKTKDDKYLLDMQRVTGPQLLFLEFCAAFLTNLRVL 502



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>KIN10_ARATH (Q38997) SNF1-related protein kinase catalytic alpha subunit KIN10|
           (EC 2.7.11.1) (AKIN10) (AKIN alpha2)
          Length = 535

 Score =  211 bits (536), Expect = 2e-54
 Identities = 109/179 (60%), Positives = 134/179 (74%), Gaps = 3/179 (1%)
 Frame = -3

Query: 702 RATSGYLGADYQQSMGRSLNQFTSSESASPSTRQYLPGTNDSQGSGLRPYYPVERKWALG 523
           RA+SGYLGA++Q++M  +     +   ASP + + LPG  + QG GLR  YPVERKWALG
Sbjct: 359 RASSGYLGAEFQETMEGTPRMHPAESVASPVSHR-LPGLMEYQGVGLRSQYPVERKWALG 417

Query: 522 LQSRAHPREIMIEVLKALQELNVSWKKNGHYNMKCRWCPGFP---QVSDMLDANHSFVDD 352
           LQSRAHPREIM EVLKALQ+LNV WKK GHYNMKCRW P       +S+ +  N+ F D+
Sbjct: 418 LQSRAHPREIMTEVLKALQDLNVCWKKIGHYNMKCRWVPNSSADGMLSNSMHDNNYFGDE 477

Query: 351 STIMDNGDANGRLPAVIKFEIQLYKTKDDKYLLDMQRLTGPQLLFLDFCAAFLTNLRVL 175
           S+I++N +A  + P V+KFEIQLYKT+DDKYLLD+QR+ GPQ LFLD CAAFL  LRVL
Sbjct: 478 SSIIEN-EAAVKSPNVVKFEIQLYKTRDDKYLLDLQRVQGPQFLFLDLCAAFLAQLRVL 535



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>KIN11_ARATH (P92958) SNF1-related protein kinase catalytic alpha subunit KIN11|
           (EC 2.7.11.1) (AKIN11) (AKIN alpha1)
          Length = 512

 Score =  187 bits (476), Expect = 2e-47
 Identities = 95/177 (53%), Positives = 121/177 (68%), Gaps = 1/177 (0%)
 Frame = -3

Query: 702 RATSGYLGADYQQSMGRSLNQFTSSESASPSTRQYLPGTNDSQGSGLRPYYPVERKWALG 523
           R  SGYL +++Q++     N   + E+ +     ++P   D  G G R   PV+RKWALG
Sbjct: 337 RVPSGYLESEFQETTDSGSNPMRTPEAGASPVGHWIPAHVDHYGLGARSQVPVDRKWALG 396

Query: 522 LQSRAHPREIMIEVLKALQELNVSWKKNGHYNMKCRWCPGFPQ-VSDMLDANHSFVDDST 346
           LQS AHPREIM EVLKALQELNV WKK GHYNMKCRW PG     + M++    F D+S+
Sbjct: 397 LQSHAHPREIMNEVLKALQELNVCWKKIGHYNMKCRWVPGLADGQNTMVNNQLHFRDESS 456

Query: 345 IMDNGDANGRLPAVIKFEIQLYKTKDDKYLLDMQRLTGPQLLFLDFCAAFLTNLRVL 175
           I+++ D     P VIKFE+QLYK +++KYLLD+QR+ GPQ LFLD CAAFLT LRV+
Sbjct: 457 IIED-DCAMTSPTVIKFELQLYKAREEKYLLDIQRVNGPQFLFLDLCAAFLTELRVI 512



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>AAPK2_CAEEL (Q95ZQ4) 5'-AMP-activated protein kinase catalytic subunit alpha-2|
           (EC 2.7.11.1) (AMP-activated kinase 2)
          Length = 626

 Score = 45.1 bits (105), Expect = 2e-04
 Identities = 27/100 (27%), Positives = 43/100 (43%)
 Frame = -3

Query: 537 KWALGLQSRAHPREIMIEVLKALQELNVSWKKNGHYNMKCRWCPGFPQVSDMLDANHSFV 358
           KW LG++S++ P +IM EV +A+++L++ WK    Y++  R  P  P             
Sbjct: 464 KWHLGIRSQSRPEDIMFEVFRAMKQLDMEWKVLNPYHVIVRRKPDAPAADPP-------- 515

Query: 357 DDSTIMDNGDANGRLPAVIKFEIQLYKTKDDKYLLDMQRL 238
                              K  +QLY+     YLLD + L
Sbjct: 516 -------------------KMSLQLYQVDQRSYLLDFKSL 536



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>SNF1_CANGA (Q00372) Carbon catabolite derepressing protein kinase (EC|
           2.7.11.1)
          Length = 612

 Score = 44.7 bits (104), Expect = 3e-04
 Identities = 26/101 (25%), Positives = 54/101 (53%), Gaps = 3/101 (2%)
 Frame = -3

Query: 537 KWALGLQSRAHPREIMIEVLKALQELNVSWKKNGH---YNMKCRWCPGFPQVSDMLDANH 367
           +W  G++SR++P ++M E+  AL+ L   W K      + ++ RW           D+  
Sbjct: 480 RWHFGIRSRSYPLDVMGEIYIALKNLGAEWAKPSEEDLWTIRVRW---------KYDS-- 528

Query: 366 SFVDDSTIMDNGDANGRLPAVIKFEIQLYKTKDDKYLLDMQ 244
              D+S ++++G    ++P ++K  IQL++ + + YL+D +
Sbjct: 529 ---DESRLIEDGVK--KIPNLMKIVIQLFQIETNNYLVDFK 564



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>SNF1_YEAST (P06782) Carbon catabolite derepressing protein kinase (EC|
           2.7.11.1)
          Length = 633

 Score = 44.3 bits (103), Expect = 4e-04
 Identities = 25/101 (24%), Positives = 48/101 (47%), Gaps = 3/101 (2%)
 Frame = -3

Query: 537 KWALGLQSRAHPREIMIEVLKALQELNVSWKKNGH---YNMKCRWCPGFPQVSDMLDANH 367
           +W  G++SR++P ++M E+  AL+ L   W K      + +K RW               
Sbjct: 504 RWHFGIRSRSYPLDVMGEIYIALKNLGAEWAKPSEEDLWTIKLRW--------------- 548

Query: 366 SFVDDSTIMDNGDANGRLPAVIKFEIQLYKTKDDKYLLDMQ 244
                  I +  + N ++P ++K  IQL++ + + YL+D +
Sbjct: 549 ----KYDIGNKTNTNEKIPDLMKMVIQLFQIETNNYLVDFK 585



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>AAPK2_RAT (Q09137) 5'-AMP-activated protein kinase catalytic subunit alpha-2|
           (EC 2.7.11.1) (AMPK alpha-2 chain)
          Length = 552

 Score = 42.0 bits (97), Expect = 0.002
 Identities = 22/68 (32%), Positives = 38/68 (55%)
 Frame = -3

Query: 618 SPSTRQYLPGTNDSQGSGLRPYYPVERKWALGLQSRAHPREIMIEVLKALQELNVSWKKN 439
           SP  R  L   N ++   L      + KW LG++S++ P +IM EV +A+++L+  WK  
Sbjct: 377 SPKARCPLDALNTTKPKSLAVK---KAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVV 433

Query: 438 GHYNMKCR 415
             Y+++ R
Sbjct: 434 NAYHLRVR 441



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>AAPK2_PONPY (Q5RD00) 5'-AMP-activated protein kinase catalytic subunit alpha-2|
           (EC 2.7.11.1) (AMPK alpha-2 chain)
          Length = 552

 Score = 42.0 bits (97), Expect = 0.002
 Identities = 22/68 (32%), Positives = 38/68 (55%)
 Frame = -3

Query: 618 SPSTRQYLPGTNDSQGSGLRPYYPVERKWALGLQSRAHPREIMIEVLKALQELNVSWKKN 439
           SP  R  L   N ++   L      + KW LG++S++ P +IM EV +A+++L+  WK  
Sbjct: 377 SPKARCPLDALNTTKPKSLAVK---KAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVV 433

Query: 438 GHYNMKCR 415
             Y+++ R
Sbjct: 434 NAYHLRVR 441



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>AAPK2_PIG (Q28948) 5'-AMP-activated protein kinase catalytic subunit alpha-2|
           (EC 2.7.11.1) (AMPK alpha-2 chain)
          Length = 552

 Score = 42.0 bits (97), Expect = 0.002
 Identities = 22/68 (32%), Positives = 38/68 (55%)
 Frame = -3

Query: 618 SPSTRQYLPGTNDSQGSGLRPYYPVERKWALGLQSRAHPREIMIEVLKALQELNVSWKKN 439
           SP  R  L   N ++   L      + KW LG++S++ P +IM EV +A+++L+  WK  
Sbjct: 377 SPKARCPLDALNTTKPKSLAVK---KAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVV 433

Query: 438 GHYNMKCR 415
             Y+++ R
Sbjct: 434 NAYHLRVR 441



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>AAPK2_HUMAN (P54646) 5'-AMP-activated protein kinase catalytic subunit alpha-2|
           (EC 2.7.11.1) (AMPK alpha-2 chain)
          Length = 552

 Score = 42.0 bits (97), Expect = 0.002
 Identities = 22/68 (32%), Positives = 38/68 (55%)
 Frame = -3

Query: 618 SPSTRQYLPGTNDSQGSGLRPYYPVERKWALGLQSRAHPREIMIEVLKALQELNVSWKKN 439
           SP  R  L   N ++   L      + KW LG++S++ P +IM EV +A+++L+  WK  
Sbjct: 377 SPKARCPLDALNTTKPKSLAVK---KAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVV 433

Query: 438 GHYNMKCR 415
             Y+++ R
Sbjct: 434 NAYHLRVR 441



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>SNF1_CANAL (P52497) Carbon catabolite derepressing protein kinase (EC|
           2.7.11.1)
          Length = 620

 Score = 41.6 bits (96), Expect = 0.002
 Identities = 28/115 (24%), Positives = 52/115 (45%), Gaps = 3/115 (2%)
 Frame = -3

Query: 579 SQGSGLRPYYPVERKWALGLQSRAHPREIMIEVLKALQELNVSWKKNGH---YNMKCRWC 409
           SQ     P    + +W  G++SR++P ++M E+ +AL+ L   W K      + ++ RW 
Sbjct: 473 SQQHAPPPTKKSKTRWHFGIRSRSYPLDVMGEIYRALKNLGAEWAKPTEEELWTIRVRW- 531

Query: 408 PGFPQVSDMLDANHSFVDDSTIMDNGDANGRLPAVIKFEIQLYKTKDDKYLLDMQ 244
                            D S   + G A    P ++K +IQL++ + + YL+  +
Sbjct: 532 ---------------KYDTSAQFECGSA----PNLMKMQIQLFQLEPNNYLVSFK 567



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>AAPK1_PONPY (Q5RDH5) 5'-AMP-activated protein kinase catalytic subunit alpha-1|
           (EC 2.7.11.1) (AMPK alpha-1 chain)
          Length = 550

 Score = 41.6 bits (96), Expect = 0.002
 Identities = 23/70 (32%), Positives = 39/70 (55%), Gaps = 2/70 (2%)
 Frame = -3

Query: 618 SPSTRQYLPGTND--SQGSGLRPYYPVERKWALGLQSRAHPREIMIEVLKALQELNVSWK 445
           +P  R  L   N   S+  G+R     + KW LG++S++ P +IM EV +A+++L+  WK
Sbjct: 373 TPRARHTLDELNPQKSKHQGVR-----KAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWK 427

Query: 444 KNGHYNMKCR 415
               Y ++ R
Sbjct: 428 VVNPYYLRVR 437



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>AAPK1_HUMAN (Q13131) 5'-AMP-activated protein kinase catalytic subunit alpha-1|
           (EC 2.7.11.1) (AMPK alpha-1 chain)
          Length = 550

 Score = 41.6 bits (96), Expect = 0.002
 Identities = 23/70 (32%), Positives = 39/70 (55%), Gaps = 2/70 (2%)
 Frame = -3

Query: 618 SPSTRQYLPGTND--SQGSGLRPYYPVERKWALGLQSRAHPREIMIEVLKALQELNVSWK 445
           +P  R  L   N   S+  G+R     + KW LG++S++ P +IM EV +A+++L+  WK
Sbjct: 373 TPRARHTLDELNPQKSKHQGVR-----KAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWK 427

Query: 444 KNGHYNMKCR 415
               Y ++ R
Sbjct: 428 VVNPYYLRVR 437



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>AAPK1_RAT (P54645) 5'-AMP-activated protein kinase catalytic subunit alpha-1|
           (EC 2.7.11.1) (AMPK alpha-1 chain)
          Length = 548

 Score = 41.6 bits (96), Expect = 0.002
 Identities = 23/70 (32%), Positives = 39/70 (55%), Gaps = 2/70 (2%)
 Frame = -3

Query: 618 SPSTRQYLPGTND--SQGSGLRPYYPVERKWALGLQSRAHPREIMIEVLKALQELNVSWK 445
           +P  R  L   N   S+  G+R     + KW LG++S++ P +IM EV +A+++L+  WK
Sbjct: 371 TPRARHTLDELNPQKSKHQGVR-----KAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWK 425

Query: 444 KNGHYNMKCR 415
               Y ++ R
Sbjct: 426 VVNPYYLRVR 435



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>AAPK1_MOUSE (Q5EG47) 5'-AMP-activated protein kinase catalytic subunit alpha-1|
           (EC 2.7.11.1) (AMPK alpha-1 chain)
          Length = 548

 Score = 41.6 bits (96), Expect = 0.002
 Identities = 23/70 (32%), Positives = 39/70 (55%), Gaps = 2/70 (2%)
 Frame = -3

Query: 618 SPSTRQYLPGTND--SQGSGLRPYYPVERKWALGLQSRAHPREIMIEVLKALQELNVSWK 445
           +P  R  L   N   S+  G+R     + KW LG++S++ P +IM EV +A+++L+  WK
Sbjct: 371 TPRARHTLDELNPQKSKHQGVR-----KAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWK 425

Query: 444 KNGHYNMKCR 415
               Y ++ R
Sbjct: 426 VVNPYYLRVR 435



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>SNF1_CANTR (O94168) Carbon catabolite derepressing protein kinase (EC|
           2.7.11.1)
          Length = 619

 Score = 40.0 bits (92), Expect = 0.007
 Identities = 25/110 (22%), Positives = 51/110 (46%), Gaps = 4/110 (3%)
 Frame = -3

Query: 561 RPYYPVERKWALGLQSRAHPREIMIEVLKALQELNVSWKKNGH---YNMKCRW-CPGFPQ 394
           +P   ++ +W  G++SR++P ++M E+ +AL+ L   W K      + ++ RW     PQ
Sbjct: 478 QPTKKLKTRWHFGIRSRSYPLDVMGEIYRALKNLGAEWAKPTEEELWTIRVRWKYDSTPQ 537

Query: 393 VSDMLDANHSFVDDSTIMDNGDANGRLPAVIKFEIQLYKTKDDKYLLDMQ 244
           +      N                     ++K +IQL++ + + YL+D +
Sbjct: 538 LRVWQRTN---------------------LMKMQIQLFQLEPNNYLVDFK 566



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>AHPF_PSEPU (P0A156) Alkyl hydroperoxide reductase subunit F (EC 1.6.4.-)|
          Length = 520

 Score = 30.8 bits (68), Expect = 4.2
 Identities = 15/67 (22%), Positives = 31/67 (46%), Gaps = 5/67 (7%)
 Frame = -3

Query: 522 LQSRAHPREIMIEVLKALQELNVSWKKNGHYNMKCRWCPGFPQVSDML-----DANHSFV 358
           LQ   HP +   EV++ +Q L   +    ++++ C+ CP   Q  +++     +  H  +
Sbjct: 94  LQVGGHPSKASAEVIEQIQALEGEFNFETYFSLSCQNCPDVVQALNLMAVLNPNVRHVAI 153

Query: 357 DDSTIMD 337
           D +   D
Sbjct: 154 DGALFQD 160



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>AHPF_PSEPK (P0A155) Alkyl hydroperoxide reductase subunit F (EC 1.6.4.-)|
          Length = 520

 Score = 30.8 bits (68), Expect = 4.2
 Identities = 15/67 (22%), Positives = 31/67 (46%), Gaps = 5/67 (7%)
 Frame = -3

Query: 522 LQSRAHPREIMIEVLKALQELNVSWKKNGHYNMKCRWCPGFPQVSDML-----DANHSFV 358
           LQ   HP +   EV++ +Q L   +    ++++ C+ CP   Q  +++     +  H  +
Sbjct: 94  LQVGGHPSKASAEVIEQIQALEGEFNFETYFSLSCQNCPDVVQALNLMAVLNPNVRHVAI 153

Query: 357 DDSTIMD 337
           D +   D
Sbjct: 154 DGALFQD 160



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>CDC23_YEAST (P16522) Anaphase-promoting complex subunit CDC23 (Cell division|
           control protein 23)
          Length = 626

 Score = 30.8 bits (68), Expect = 4.2
 Identities = 29/118 (24%), Positives = 49/118 (41%), Gaps = 15/118 (12%)
 Frame = -3

Query: 498 EIMIEVLKALQELNVSWKKNGHYNMKCRWCPGFPQVSDMLDANHSFVDDSTIMDNGDANG 319
           +I I++ KA  EL+  WK  G          G  +  D+ D  HS  D+S + +      
Sbjct: 12  DIRIQLRKAATELS-RWKLYGSSKWAAEALAGLAEAIDV-DQTHSLADESPLRNKQGVPK 69

Query: 318 RLPAVIKFEIQLYKTKDDKYLLD---------------MQRLTGPQLLFLDFCAAFLT 190
           ++  + +    L +T+ D YLL                ++ +T P L FL   + FL+
Sbjct: 70  QMFEIPQNGFGLSETEYDLYLLGSTLFDAKEFDRCVFFLKDVTNPYLKFLKLYSKFLS 127



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>SYS_FUSNN (Q8RH11) Seryl-tRNA synthetase (EC 6.1.1.11) (Serine--tRNA ligase)|
           (SerRS)
          Length = 421

 Score = 30.4 bits (67), Expect = 5.5
 Identities = 14/32 (43%), Positives = 19/32 (59%)
 Frame = -3

Query: 330 DANGRLPAVIKFEIQLYKTKDDKYLLDMQRLT 235
           +  G+LP   KFE  +YKT DD YL+    +T
Sbjct: 205 EGTGQLP---KFEEDMYKTTDDMYLISTSEIT 233



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>K0329_HUMAN (O15040) Protein KIAA0329/KIAA0297|
          Length = 1411

 Score = 29.6 bits (65), Expect = 9.4
 Identities = 16/33 (48%), Positives = 20/33 (60%), Gaps = 2/33 (6%)
 Frame = -3

Query: 648 LNQFT--SSESASPSTRQYLPGTNDSQGSGLRP 556
           LN  T  SS S +PS  Q+LPGT   +GS + P
Sbjct: 645 LNSLTVPSSLSWAPSAEQWLPGTRADEGSPVEP 677


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 105,187,472
Number of Sequences: 219361
Number of extensions: 2248710
Number of successful extensions: 5463
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 5282
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5454
length of database: 80,573,946
effective HSP length: 109
effective length of database: 56,663,597
effective search space used: 7082949625
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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