ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd3f18
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1TIM22_YEAST (Q12328) Mitochondrial import inner membrane translo... 45 3e-04
2TIM22_CRYNE (Q5KKL8) Mitochondrial import inner membrane translo... 40 0.008
3TIM17_CAEEL (O44477) Probable mitochondrial import inner membran... 38 0.024
4TIM22_KLULA (Q6CRJ6) Mitochondrial import inner membrane translo... 37 0.041
5TIM22_YARLI (Q6BZY4) Mitochondrial import inner membrane translo... 37 0.041
6TIM22_ASHGO (Q75E80) Mitochondrial import inner membrane translo... 37 0.054
7TIM22_CAEEL (Q9NAQ9) Mitochondrial import inner membrane translo... 35 0.20
8TIM22_NEUCR (Q9C1E8) Mitochondrial import inner membrane translo... 35 0.27
9EMAL_CAEEL (O45487) Echinoderm microtubule-associated protein-li... 34 0.35
10TIM22_DEBHA (Q6BT35) Mitochondrial import inner membrane translo... 34 0.35
11NUJM_NEUCR (P25710) NADH-ubiquinone oxidoreductase 21.3 kDa subu... 33 0.78
12CLCN6_RABIT (Q9TT16) Chloride channel protein 6 (ClC-6) 30 5.0
13RL25_BACSK (Q5WAD7) 50S ribosomal protein L25 (General stress pr... 30 8.6

>TIM22_YEAST (Q12328) Mitochondrial import inner membrane translocase subunit|
           TIM22
          Length = 207

 Score = 44.7 bits (104), Expect = 3e-04
 Identities = 24/65 (36%), Positives = 37/65 (56%)
 Frame = -1

Query: 473 FAAVGGLYIGVEQIVQSQRKKRDFVNGAIGAFVSGATVYGYRGKSIKSALIGGSSLAFTS 294
           F  +G +Y GVE +++S R K D  NG    F +GA +    G   ++AL+GG+  A  S
Sbjct: 129 FGYIGMIYAGVECVIESLRAKNDIYNGVTAGFFTGAGLAYKAGP--QAALMGGAGFAAFS 186

Query: 293 AILDV 279
           A +D+
Sbjct: 187 AAIDL 191



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>TIM22_CRYNE (Q5KKL8) Mitochondrial import inner membrane translocase subunit|
           TIM22
          Length = 187

 Score = 39.7 bits (91), Expect = 0.008
 Identities = 26/69 (37%), Positives = 37/69 (53%), Gaps = 2/69 (2%)
 Frame = -1

Query: 488 SYGVTFAAVGGLYIGVEQIVQSQRKKRDFVNGAIGAFVSGATVYGYRGKSIKSALIGG-- 315
           S G  FA VG +Y GVE  ++  R K D  NG    F++GA +    G    +A++GG  
Sbjct: 110 SSGRGFAKVGMVYSGVECCIEGYRAKNDIYNGVSAGFLTGAILARNAG---PTAMLGGGV 166

Query: 314 SSLAFTSAI 288
           +  AF+ AI
Sbjct: 167 AFAAFSGAI 175



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>TIM17_CAEEL (O44477) Probable mitochondrial import inner membrane translocase|
           subunit Tim17
          Length = 181

 Score = 38.1 bits (87), Expect = 0.024
 Identities = 30/91 (32%), Positives = 49/91 (53%), Gaps = 3/91 (3%)
 Frame = -1

Query: 524 LPGLVRTLKMCGSY-GVTFAAVGGLYIGVEQIVQSQRKKRDFVNGAIGAFVSGATVYGYR 348
           L G++R ++M  +  GV FAA GG++  ++  + + RKK D +N  +   ++GA +    
Sbjct: 46  LVGMMREVRMRSTLTGVQFAAWGGMFSTIDCCLVAIRKKEDPINSIVSGGLTGALLAIRS 105

Query: 347 GKSIK--SALIGGSSLAFTSAILDVGGNTTR 261
           G  +   SA++G   LA    I  VG  TTR
Sbjct: 106 GPKVMAGSAILGSVILAM---IEGVGLVTTR 133



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>TIM22_KLULA (Q6CRJ6) Mitochondrial import inner membrane translocase subunit|
           TIM22
          Length = 196

 Score = 37.4 bits (85), Expect = 0.041
 Identities = 23/66 (34%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
 Frame = -1

Query: 473 FAAVGGLYIGVEQIVQSQRKKRDFVNG-AIGAFVSGATVYGYRGKSIKSALIGGSSLAFT 297
           F  +G +Y GVE +V+S R K D  NG A G    G   Y       ++AL+G +  A  
Sbjct: 118 FGYIGMIYSGVECVVESLRAKNDIYNGVAAGCLTGGGLAY---KSGPQAALVGCAGFAAF 174

Query: 296 SAILDV 279
           S  +D+
Sbjct: 175 STAIDL 180



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>TIM22_YARLI (Q6BZY4) Mitochondrial import inner membrane translocase subunit|
           TIM22
          Length = 189

 Score = 37.4 bits (85), Expect = 0.041
 Identities = 23/74 (31%), Positives = 36/74 (48%)
 Frame = -1

Query: 500 KMCGSYGVTFAAVGGLYIGVEQIVQSQRKKRDFVNGAIGAFVSGATVYGYRGKSIKSALI 321
           K   S    F  +GG++ G E  ++S R K D  NG     ++G  +    G   ++AL+
Sbjct: 102 KRAWSSAKNFGFIGGVFSGTECCIESLRAKNDIWNGVAAGCLTGGGLAVKAGP--QAALV 159

Query: 320 GGSSLAFTSAILDV 279
           G +  A  SA +DV
Sbjct: 160 GCAGFAAFSAAIDV 173



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>TIM22_ASHGO (Q75E80) Mitochondrial import inner membrane translocase subunit|
           TIM22
          Length = 201

 Score = 37.0 bits (84), Expect = 0.054
 Identities = 25/76 (32%), Positives = 38/76 (50%)
 Frame = -1

Query: 473 FAAVGGLYIGVEQIVQSQRKKRDFVNGAIGAFVSGATVYGYRGKSIKSALIGGSSLAFTS 294
           F  +G +Y GVE  ++S R K D  NG     ++G  +    G S  +ALIG +  A  S
Sbjct: 123 FGYIGMIYSGVECTIESLRAKNDLYNGVAAGCLTGGGLAYKSGPS--AALIGCAGFAAFS 180

Query: 293 AILDVGGNTTRVDNGK 246
             +D+     R +NG+
Sbjct: 181 TAIDL---YMRSENGR 193



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>TIM22_CAEEL (Q9NAQ9) Mitochondrial import inner membrane translocase subunit|
           tim-22
          Length = 213

 Score = 35.0 bits (79), Expect = 0.20
 Identities = 24/71 (33%), Positives = 35/71 (49%), Gaps = 2/71 (2%)
 Frame = -1

Query: 488 SYGVTFAAVGGLYIGVEQIVQSQRKKRDFVNGAIGAFVSGATVYGYRG--KSIKSALIGG 315
           SYG  F ++G ++ G E  +++ R K D+ NG      SG  V G  G    I  A+ G 
Sbjct: 142 SYGKNFGSIGLMFSGTECALETIRAKSDWRNGT----YSGGIVGGLLGLRAGIMPAVWGA 197

Query: 314 SSLAFTSAILD 282
           +  A  S I+D
Sbjct: 198 AGFAVFSTIID 208



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>TIM22_NEUCR (Q9C1E8) Mitochondrial import inner membrane translocase subunit|
           tim-22
          Length = 194

 Score = 34.7 bits (78), Expect = 0.27
 Identities = 21/64 (32%), Positives = 33/64 (51%)
 Frame = -1

Query: 473 FAAVGGLYIGVEQIVQSQRKKRDFVNGAIGAFVSGATVYGYRGKSIKSALIGGSSLAFTS 294
           FA VG L+ G+E  ++  R K D  NG     ++GA +    G   ++A +G +  A  S
Sbjct: 123 FAKVGALFSGIECGIEGLRAKNDLGNGVAAGCLTGAILAKNGGP--QAAAVGCAGFAAFS 180

Query: 293 AILD 282
           A +D
Sbjct: 181 AAID 184



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>EMAL_CAEEL (O45487) Echinoderm microtubule-associated protein-like elp-1|
          Length = 891

 Score = 34.3 bits (77), Expect = 0.35
 Identities = 24/76 (31%), Positives = 36/76 (47%), Gaps = 3/76 (3%)
 Frame = -1

Query: 362 VYGYRGKSIKS--ALIGGSSLAFTSAILDVGGNTTRVDNG-KAYHAYTMEKKPEPAH*PR 192
           VY YRGK ++S   ++    L F SA L V  N T  D   + YH +T + K    H  +
Sbjct: 285 VYSYRGKDVRSNIEMLPTGELVFFSANLVVLMNITGEDRSQRIYHGHTCDVKCITLHPNK 344

Query: 191 LCTCSTNHANHQLGEF 144
           +   S   + H + +F
Sbjct: 345 ILVASGQSSCHSVEKF 360



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>TIM22_DEBHA (Q6BT35) Mitochondrial import inner membrane translocase subunit|
           TIM22
          Length = 182

 Score = 34.3 bits (77), Expect = 0.35
 Identities = 20/65 (30%), Positives = 33/65 (50%)
 Frame = -1

Query: 473 FAAVGGLYIGVEQIVQSQRKKRDFVNGAIGAFVSGATVYGYRGKSIKSALIGGSSLAFTS 294
           F  +G +Y GVE  ++S R K D  NG     ++G  +    G   ++AL+G +  A  S
Sbjct: 104 FGYIGMVYSGVECAIESLRAKHDIYNGVSAGCITGGGLAIRAGP--QAALVGCAGFAAFS 161

Query: 293 AILDV 279
             +D+
Sbjct: 162 TAIDL 166



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>NUJM_NEUCR (P25710) NADH-ubiquinone oxidoreductase 21.3 kDa subunit (EC|
           1.6.5.3) (EC 1.6.99.3)
          Length = 200

 Score = 33.1 bits (74), Expect = 0.78
 Identities = 15/40 (37%), Positives = 24/40 (60%)
 Frame = -1

Query: 479 VTFAAVGGLYIGVEQIVQSQRKKRDFVNGAIGAFVSGATV 360
           ++F   GG+Y   +Q   + R+K D  N AIGAF+ G+ +
Sbjct: 59  ISFTLAGGIYRFAQQASANLREKEDGWNHAIGAFLGGSVM 98



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>CLCN6_RABIT (Q9TT16) Chloride channel protein 6 (ClC-6)|
          Length = 869

 Score = 30.4 bits (67), Expect = 5.0
 Identities = 22/83 (26%), Positives = 33/83 (39%), Gaps = 30/83 (36%)
 Frame = -1

Query: 524 LPGLVRTLKM-CGSYGVTFAAVGGLYIGVE-----------------------------Q 435
           +PG+VR   + C  +GV F+  GGL++G E                              
Sbjct: 171 VPGIVRLRTLLCKVFGVLFSVAGGLFVGKEGPMIHSGAVVGAGLPQFQSISLRKIQFNFP 230

Query: 434 IVQSQRKKRDFVNGAIGAFVSGA 366
             +S R KRDFV+    A ++ A
Sbjct: 231 YFRSDRDKRDFVSAGAAAGIAAA 253



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>RL25_BACSK (Q5WAD7) 50S ribosomal protein L25 (General stress protein CTC)|
          Length = 218

 Score = 29.6 bits (65), Expect = 8.6
 Identities = 15/50 (30%), Positives = 25/50 (50%), Gaps = 3/50 (6%)
 Frame = -1

Query: 347 GKSIKSALIGGSSLAFTSAILDVGGN---TTRVDNGKAYHAYTMEKKPEP 207
           GK +KS  +   ++AF     DVG N     +++NG  +H    E + +P
Sbjct: 32  GKKVKSTPVSVEAVAFLKTFRDVGQNGLFDLQLENGGKHHVMVQEVQIDP 81


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 78,929,108
Number of Sequences: 219361
Number of extensions: 1451832
Number of successful extensions: 4721
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 4540
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4719
length of database: 80,573,946
effective HSP length: 108
effective length of database: 56,882,958
effective search space used: 6484657212
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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