ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd2o15
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1RPE65_HUMAN (Q16518) Retinal pigment epithelium-specific 65 kDa ... 47 3e-05
2RPE65_CERAE (Q9XT71) Retinal pigment epithelium-specific 65 kDa ... 47 3e-05
3RPE65_BOVIN (Q28175) Retinal pigment epithelium-specific 65 kDa ... 46 6e-05
4RPE65_CHICK (Q9YGX2) Retinal pigment epithelium-specific 65 kDa ... 45 1e-04
5RPE65_MOUSE (Q91ZQ5) Retinal pigment epithelium-specific 65 kDa ... 45 1e-04
6RPE65_RAT (O70276) Retinal pigment epithelium-specific 65 kDa pr... 45 2e-04
7RPE65_CANFA (Q9TVB8) Retinal pigment epithelium-specific 65 kDa ... 44 4e-04
8RPE65_AMBTI (Q9YI25) Retinal pigment epithelium-specific 65 kDa ... 43 5e-04
9RPE65_CYNPY (Q8AXN9) Retinal pigment epithelium-specific 65 kDa ... 41 0.002
10BCDO2_HUMAN (Q9BYV7) Beta,beta-carotene 9',10'-dioxygenase (EC 1... 40 0.004
11BCDO2_MACFA (Q8HXG8) Beta,beta-carotene 9',10'-dioxygenase (EC 1... 40 0.005
12BCDO2_MOUSE (Q99NF1) Beta,beta-carotene 9',10'-dioxygenase (EC 1... 37 0.035
13PACC_CANAL (Q9UW14) pH-response transcription factor pacC/RIM101... 31 1.9
14ERFB_YARLI (Q6C890) Palmitoyltransferase ERF2 (EC 2.3.1.-) (DHHC... 31 2.5
15RF3_XANCP (Q8P6V6) Peptide chain release factor 3 (RF-3) 30 4.3
16AROC_VIBAN (P39198) Chorismate synthase (EC 4.2.3.5) (5-enolpyru... 30 4.3
17RSE1_DEBHA (Q6BYK1) Pre-mRNA-splicing factor RSE1 30 5.6
18PYRC_NEIMB (Q9K0D1) Dihydroorotase (EC 3.5.2.3) (DHOase) 30 5.6
19PYRC_NEIMA (Q9JVD6) Dihydroorotase (EC 3.5.2.3) (DHOase) 30 5.6
20K0226_MOUSE (Q80U62) Protein KIAA0226 29 7.3
21PYRC_ARATH (O04904) Dihydroorotase, mitochondrial precursor (EC ... 29 7.3
22PYRC_SYNY3 (P74438) Dihydroorotase (EC 3.5.2.3) (DHOase) 29 7.3
23UVRA_STRMU (P72481) UvrABC system protein A (UvrA protein) (Exci... 29 9.5

>RPE65_HUMAN (Q16518) Retinal pigment epithelium-specific 65 kDa protein|
          Length = 532

 Score = 47.0 bits (110), Expect = 3e-05
 Identities = 41/142 (28%), Positives = 59/142 (41%), Gaps = 1/142 (0%)
 Frame = -2

Query: 535 GTDVAMEFPVIN-DKYVGLHHRYAYAQVVNVEGSMAGGCGTVRPKFGGFAKLYLDQEIKE 359
           G   A EFP IN  KY G  + YAY   +N               F       L+ + KE
Sbjct: 410 GPRQAFEFPQINYQKYCGKPYTYAYGLGLN--------------HFVPDRLCKLNVKTKE 455

Query: 358 SCGDSIHVEYHHLETNQFCSGAIFVAKEDALHEDDGWIISFVHDEGTNISQAHIIDARRF 179
           +        +   E + + S  IFV+  DAL EDDG ++S V   G     A+++     
Sbjct: 456 T--------WVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPAYLLILNAK 507

Query: 178 ESGPVAKITLPQRVPYGFHGAF 113
           +   VA+  +   +P  FHG F
Sbjct: 508 DLSEVARAEVEINIPVTFHGLF 529



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>RPE65_CERAE (Q9XT71) Retinal pigment epithelium-specific 65 kDa protein|
          Length = 532

 Score = 47.0 bits (110), Expect = 3e-05
 Identities = 41/142 (28%), Positives = 59/142 (41%), Gaps = 1/142 (0%)
 Frame = -2

Query: 535 GTDVAMEFPVIN-DKYVGLHHRYAYAQVVNVEGSMAGGCGTVRPKFGGFAKLYLDQEIKE 359
           G   A EFP IN  KY G  + YAY   +N               F       L+ + KE
Sbjct: 410 GPRQAFEFPQINYQKYCGKPYTYAYGLGLN--------------HFVPDRLCKLNVKTKE 455

Query: 358 SCGDSIHVEYHHLETNQFCSGAIFVAKEDALHEDDGWIISFVHDEGTNISQAHIIDARRF 179
           +        +   E + + S  IFV+  DAL EDDG ++S V   G     A+++     
Sbjct: 456 T--------WVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPAYLLILNAK 507

Query: 178 ESGPVAKITLPQRVPYGFHGAF 113
           +   VA+  +   +P  FHG F
Sbjct: 508 DLSEVARAEVEINIPVTFHGLF 529



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>RPE65_BOVIN (Q28175) Retinal pigment epithelium-specific 65 kDa protein|
           (Membrane receptor p63)
          Length = 532

 Score = 46.2 bits (108), Expect = 6e-05
 Identities = 41/142 (28%), Positives = 59/142 (41%), Gaps = 1/142 (0%)
 Frame = -2

Query: 535 GTDVAMEFPVIN-DKYVGLHHRYAYAQVVNVEGSMAGGCGTVRPKFGGFAKLYLDQEIKE 359
           G   A EFP IN  KY G  + YAY   +N               F       L+ + KE
Sbjct: 410 GPRQAFEFPQINYQKYGGKPYTYAYGLGLN--------------HFVPDRLCKLNVKTKE 455

Query: 358 SCGDSIHVEYHHLETNQFCSGAIFVAKEDALHEDDGWIISFVHDEGTNISQAHIIDARRF 179
           +        +   E + + S  IFV+  DAL EDDG ++S V   G     A+++     
Sbjct: 456 T--------WVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPAYLLILNAK 507

Query: 178 ESGPVAKITLPQRVPYGFHGAF 113
           +   VA+  +   +P  FHG F
Sbjct: 508 DLSEVARAEVEINIPVTFHGLF 529



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>RPE65_CHICK (Q9YGX2) Retinal pigment epithelium-specific 65 kDa protein|
          Length = 533

 Score = 45.4 bits (106), Expect = 1e-04
 Identities = 39/138 (28%), Positives = 58/138 (42%), Gaps = 1/138 (0%)
 Frame = -2

Query: 523 AMEFPVIN-DKYVGLHHRYAYAQVVNVEGSMAGGCGTVRPKFGGFAKLYLDQEIKESCGD 347
           A EFP IN  KY G  + Y Y   +N               F       L+ + KE+   
Sbjct: 415 AFEFPQINYKKYGGKPYTYTYGLGLN--------------HFVPDRLCKLNVKTKET--- 457

Query: 346 SIHVEYHHLETNQFCSGAIFVAKEDALHEDDGWIISFVHDEGTNISQAHIIDARRFESGP 167
                +   E + + S  IFV+  DAL EDDG ++S V   G+    A+++     +   
Sbjct: 458 -----WVWQEPDSYPSEPIFVSHPDALEEDDGVVLSIVISPGSGPKPAYLLILNAKDMSE 512

Query: 166 VAKITLPQRVPYGFHGAF 113
           VA+  +   +P  FHG F
Sbjct: 513 VARAEVEVNIPVTFHGLF 530



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>RPE65_MOUSE (Q91ZQ5) Retinal pigment epithelium-specific 65 kDa protein|
          Length = 532

 Score = 45.1 bits (105), Expect = 1e-04
 Identities = 39/142 (27%), Positives = 59/142 (41%), Gaps = 1/142 (0%)
 Frame = -2

Query: 535 GTDVAMEFPVIN-DKYVGLHHRYAYAQVVNVEGSMAGGCGTVRPKFGGFAKLYLDQEIKE 359
           G   A EFP IN  K+ G  + YAY   +N               F       L+ + KE
Sbjct: 410 GPRQAFEFPQINYQKFGGKPYTYAYGLGLN--------------HFVPDKLCKLNVKTKE 455

Query: 358 SCGDSIHVEYHHLETNQFCSGAIFVAKEDALHEDDGWIISFVHDEGTNISQAHIIDARRF 179
                    +   E + + S  IFV++ DAL EDDG ++S V   G     A+++     
Sbjct: 456 I--------WMWQEPDSYPSEPIFVSQPDALEEDDGVVLSVVVSPGAGQKPAYLLVLNAK 507

Query: 178 ESGPVAKITLPQRVPYGFHGAF 113
           +   +A+  +   +P  FHG F
Sbjct: 508 DLSEIARAEVETNIPVTFHGLF 529



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>RPE65_RAT (O70276) Retinal pigment epithelium-specific 65 kDa protein|
          Length = 532

 Score = 44.7 bits (104), Expect = 2e-04
 Identities = 22/69 (31%), Positives = 36/69 (52%)
 Frame = -2

Query: 319 ETNQFCSGAIFVAKEDALHEDDGWIISFVHDEGTNISQAHIIDARRFESGPVAKITLPQR 140
           E + + S  IFV++ DAL EDDG ++S V   G     A+++     +   +A+  +   
Sbjct: 461 EPDSYPSEPIFVSQPDALEEDDGVVLSVVVSPGAGQKPAYLLVLNAKDLSEIARAEVETN 520

Query: 139 VPYGFHGAF 113
           +P  FHG F
Sbjct: 521 IPVTFHGLF 529



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>RPE65_CANFA (Q9TVB8) Retinal pigment epithelium-specific 65 kDa protein|
          Length = 532

 Score = 43.5 bits (101), Expect = 4e-04
 Identities = 23/69 (33%), Positives = 35/69 (50%)
 Frame = -2

Query: 319 ETNQFCSGAIFVAKEDALHEDDGWIISFVHDEGTNISQAHIIDARRFESGPVAKITLPQR 140
           E + + S  IFV+  DAL EDDG ++S V   G     A+++     +   VA+  +   
Sbjct: 461 EPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPAYLLILNAKDLSEVARAEVEIN 520

Query: 139 VPYGFHGAF 113
           +P  FHG F
Sbjct: 521 IPVTFHGLF 529



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>RPE65_AMBTI (Q9YI25) Retinal pigment epithelium-specific 65 kDa protein|
          Length = 532

 Score = 43.1 bits (100), Expect = 5e-04
 Identities = 38/142 (26%), Positives = 59/142 (41%), Gaps = 1/142 (0%)
 Frame = -2

Query: 535 GTDVAMEFPVIN-DKYVGLHHRYAYAQVVNVEGSMAGGCGTVRPKFGGFAKLYLDQEIKE 359
           G   A EFP IN  K+ G  + YAY   +N               F       L+ + KE
Sbjct: 410 GPRQAFEFPQINYKKHGGKDYTYAYGVGLN--------------HFVPDRLSKLNVKTKE 455

Query: 358 SCGDSIHVEYHHLETNQFCSGAIFVAKEDALHEDDGWIISFVHDEGTNISQAHIIDARRF 179
           +        +   E + + S  IFV++ DA+ EDDG ++S V   G     A ++     
Sbjct: 456 T--------WVWQEPDTYPSEPIFVSQPDAIEEDDGVVLSVVISPGEGQKPAFLLILNAK 507

Query: 178 ESGPVAKITLPQRVPYGFHGAF 113
           +   +A+  +   +P  FHG F
Sbjct: 508 DMSEIARAEVDSNIPVTFHGMF 529



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>RPE65_CYNPY (Q8AXN9) Retinal pigment epithelium-specific 65 kDa protein|
          Length = 532

 Score = 40.8 bits (94), Expect = 0.002
 Identities = 19/69 (27%), Positives = 36/69 (52%)
 Frame = -2

Query: 319 ETNQFCSGAIFVAKEDALHEDDGWIISFVHDEGTNISQAHIIDARRFESGPVAKITLPQR 140
           E + + S  IFV++ DA+ EDDG ++S + + G     A ++     +   +A+  +   
Sbjct: 461 EPDSYPSEPIFVSQPDAMEEDDGVVLSVIVNPGPGQKPAFLLILNAKDMSEIARAEVDIN 520

Query: 139 VPYGFHGAF 113
           +P  FHG +
Sbjct: 521 IPVTFHGMY 529



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>BCDO2_HUMAN (Q9BYV7) Beta,beta-carotene 9',10'-dioxygenase (EC 1.14.99.-)|
           (Beta-carotene dioxygenase 2) (B-diox-II)
          Length = 556

 Score = 40.0 bits (92), Expect = 0.004
 Identities = 24/71 (33%), Positives = 36/71 (50%), Gaps = 1/71 (1%)
 Frame = -2

Query: 319 ETNQFCSGAIFVAKEDALHEDDGWIISFVHDEGTNISQ-AHIIDARRFESGPVAKITLPQ 143
           E   + S  +FV       ED G I+S V     N S    ++DA+ FE   + +  +P 
Sbjct: 486 EDGFYPSEPVFVPAPGTNEEDGGVILSVVITPNQNESNFLLVLDAKNFEE--LGRAEVPV 543

Query: 142 RVPYGFHGAFV 110
           ++PYGFHG F+
Sbjct: 544 QMPYGFHGTFI 554



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>BCDO2_MACFA (Q8HXG8) Beta,beta-carotene 9',10'-dioxygenase (EC 1.14.99.-)|
           (Beta-carotene dioxygenase 2) (B-diox-II)
          Length = 556

 Score = 39.7 bits (91), Expect = 0.005
 Identities = 24/71 (33%), Positives = 36/71 (50%), Gaps = 1/71 (1%)
 Frame = -2

Query: 319 ETNQFCSGAIFVAKEDALHEDDGWIISFVHDEGTNISQ-AHIIDARRFESGPVAKITLPQ 143
           E   + S  +FV       ED G I+S V     N S    ++DA+ FE   + +  +P 
Sbjct: 486 EDGFYPSEPVFVPVPGTNEEDGGVILSVVITPNQNESNFLLVLDAKNFEE--LGRAEVPV 543

Query: 142 RVPYGFHGAFV 110
           ++PYGFHG F+
Sbjct: 544 QMPYGFHGTFI 554



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>BCDO2_MOUSE (Q99NF1) Beta,beta-carotene 9',10'-dioxygenase (EC 1.14.99.-)|
           (Beta-carotene dioxygenase 2) (B-diox-II)
          Length = 532

 Score = 37.0 bits (84), Expect = 0.035
 Identities = 24/71 (33%), Positives = 36/71 (50%), Gaps = 1/71 (1%)
 Frame = -2

Query: 319 ETNQFCSGAIFVAKEDALHEDDGWIISFVHDEGTNISQ-AHIIDARRFESGPVAKITLPQ 143
           E   + S  +FV    A  ED G I+S V     + S    ++DA+ F    + +  +P 
Sbjct: 462 EEGFYPSEPVFVPVPGADEEDSGVILSVVITPNQSESNFLLVLDAKSFTE--LGRAEVPV 519

Query: 142 RVPYGFHGAFV 110
           ++PYGFHG FV
Sbjct: 520 QMPYGFHGTFV 530



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>PACC_CANAL (Q9UW14) pH-response transcription factor pacC/RIM101 (pH-response|
           regulator protein 2)
          Length = 661

 Score = 31.2 bits (69), Expect = 1.9
 Identities = 16/44 (36%), Positives = 20/44 (45%)
 Frame = +2

Query: 404 LWSHCSTTTSHTPFHVHHLCVCVPVMQANILVIDNREFHSDICP 535
           LW +C TTT        HL V VP+          + FH D+CP
Sbjct: 247 LWENCGTTTVKRDHITSHLRVHVPL----------KPFHCDLCP 280



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>ERFB_YARLI (Q6C890) Palmitoyltransferase ERF2 (EC 2.3.1.-) (DHHC cysteine-rich|
           domain-containing protein ERF2) (Ras protein
           acyltransferase)
          Length = 408

 Score = 30.8 bits (68), Expect = 2.5
 Identities = 14/34 (41%), Positives = 18/34 (52%), Gaps = 5/34 (14%)
 Frame = +2

Query: 371 LVKI*FCKTSKLW-----SHCSTTTSHTPFHVHH 457
           LV + +C T K+W     SHCS   +   FH HH
Sbjct: 205 LVYLKYCSTCKIWRPPRASHCSDCDNCVDFHDHH 238



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>RF3_XANCP (Q8P6V6) Peptide chain release factor 3 (RF-3)|
          Length = 534

 Score = 30.0 bits (66), Expect = 4.3
 Identities = 20/69 (28%), Positives = 31/69 (44%), Gaps = 6/69 (8%)
 Frame = +1

Query: 247 SIHHPHVAHPLSPQKL--LLSRIDLFQGGGTPHGLNLHK----TP*FLGQDIVLQNLQTL 408
           S+  P +A  +  Q L  L   ++L QG   P  L+ ++    TP F G  +    +Q L
Sbjct: 207 SLDAPGLAEKIGTQMLDELREELELVQGASNPFDLDAYRAGQQTPVFFGSGVNNFGVQPL 266

Query: 409 VSLFHNHQP 435
           +  F  H P
Sbjct: 267 LDFFVEHAP 275



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>AROC_VIBAN (P39198) Chorismate synthase (EC 4.2.3.5)|
           (5-enolpyruvylshikimate-3-phosphate phospholyase)
          Length = 361

 Score = 30.0 bits (66), Expect = 4.3
 Identities = 30/104 (28%), Positives = 42/104 (40%), Gaps = 17/104 (16%)
 Frame = -2

Query: 517 EFPVINDKYVGLHHRYAYAQVVNVEGSMAGGCGT-----VRPKFGGFAKLYLDQE----- 368
           ++  I DK+   H  Y Y Q   +     GG  +     +R   G  AK YL QE     
Sbjct: 94  DYSDIKDKFRPGHADYTYHQKYGIRDYRGGGRSSARETAMRVAAGAIAKKYLKQEFGVEI 153

Query: 367 ------IKESCGDSIHVEYHHLETNQ-FCSGAIFVAKEDALHED 257
                 + + C D   V+++ +E N  FC  A  VA  D L  D
Sbjct: 154 RAYLSQMGDVCIDK--VDWNEIENNAFFCPDADKVAAFDQLIRD 195



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>RSE1_DEBHA (Q6BYK1) Pre-mRNA-splicing factor RSE1|
          Length = 1256

 Score = 29.6 bits (65), Expect = 5.6
 Identities = 13/34 (38%), Positives = 20/34 (58%)
 Frame = +3

Query: 342 IESPQDSLISWSRYSFAKPPNFGLTVPQPPAILP 443
           ++SP D+ IS S Y +     +G+T P P  +LP
Sbjct: 813 VDSPGDNEISSSSYIYIIESEYGITSPFPVTVLP 846



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>PYRC_NEIMB (Q9K0D1) Dihydroorotase (EC 3.5.2.3) (DHOase)|
          Length = 344

 Score = 29.6 bits (65), Expect = 5.6
 Identities = 14/26 (53%), Positives = 18/26 (69%)
 Frame = +1

Query: 262 HVAHPLSPQKLLLSRIDLFQGGGTPH 339
           +VA  ++PQ LLL+R DL  GG  PH
Sbjct: 189 NVAATVTPQHLLLNRNDLLVGGVRPH 214



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>PYRC_NEIMA (Q9JVD6) Dihydroorotase (EC 3.5.2.3) (DHOase)|
          Length = 344

 Score = 29.6 bits (65), Expect = 5.6
 Identities = 14/26 (53%), Positives = 18/26 (69%)
 Frame = +1

Query: 262 HVAHPLSPQKLLLSRIDLFQGGGTPH 339
           +VA  ++PQ LLL+R DL  GG  PH
Sbjct: 189 NVAATVTPQHLLLNRNDLLVGGVRPH 214



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>K0226_MOUSE (Q80U62) Protein KIAA0226|
          Length = 956

 Score = 29.3 bits (64), Expect = 7.3
 Identities = 23/83 (27%), Positives = 32/83 (38%), Gaps = 18/83 (21%)
 Frame = -2

Query: 448 VEGSMAGGCGTVRPKFGGFAKLYLDQEIKESCGDSIHVEYHHLETNQFC----------- 302
           VEG ++  C  V  K+GG  +L         C D  ++ YH L  +Q C           
Sbjct: 37  VEGLVSANCPNVWSKYGGLERL---------CRDMQNILYHGLIHDQVCCRQADYWQFVK 87

Query: 301 -------SGAIFVAKEDALHEDD 254
                    A+ V K  +LHE D
Sbjct: 88  DIRWLSPHSALHVEKFISLHESD 110



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>PYRC_ARATH (O04904) Dihydroorotase, mitochondrial precursor (EC 3.5.2.3)|
           (DHOase)
          Length = 377

 Score = 29.3 bits (64), Expect = 7.3
 Identities = 14/25 (56%), Positives = 17/25 (68%)
 Frame = +1

Query: 265 VAHPLSPQKLLLSRIDLFQGGGTPH 339
           V   ++PQ LLL+R  LFQGG  PH
Sbjct: 224 VGATVTPQHLLLNRNALFQGGLQPH 248



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>PYRC_SYNY3 (P74438) Dihydroorotase (EC 3.5.2.3) (DHOase)|
          Length = 342

 Score = 29.3 bits (64), Expect = 7.3
 Identities = 13/26 (50%), Positives = 18/26 (69%)
 Frame = +1

Query: 262 HVAHPLSPQKLLLSRIDLFQGGGTPH 339
           ++A  ++PQ LL SR  LF+GG  PH
Sbjct: 189 NIAATITPQHLLFSRNALFKGGICPH 214



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>UVRA_STRMU (P72481) UvrABC system protein A (UvrA protein) (Excinuclease ABC|
           subunit A)
          Length = 943

 Score = 28.9 bits (63), Expect = 9.5
 Identities = 25/90 (27%), Positives = 38/90 (42%), Gaps = 2/90 (2%)
 Frame = -2

Query: 370 EIKESCGDSIHVEYHHLETNQFCSGAIFVAKEDALHEDDGWIISFVHDEGTNISQAHIID 191
           E+ ++   +I V    L       G +F + E ALH  DG++I    D+   I   H   
Sbjct: 192 ELSKNKMHNIEVVVDRLVQKDGIRGRLFDSIEAALHLGDGYVIIDTMDDHELIFSEHY-- 249

Query: 190 ARRFESGPVAKITLPQRVP--YGFHGAFVS 107
                S PV   T+P+  P  + F+  F S
Sbjct: 250 -----SCPVCGFTVPELEPRLFSFNAPFGS 274


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,170,841
Number of Sequences: 219361
Number of extensions: 1913325
Number of successful extensions: 5543
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 5358
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5543
length of database: 80,573,946
effective HSP length: 105
effective length of database: 57,541,041
effective search space used: 4200495993
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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